Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 735,234 Δ1 bp 2.4% intergenic (‑140/‑345) bchF ← / → ppaA 2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034735,2340C→. 2.4% 7.2 0.0 ‑0.1212intergenic (‑140/‑345)bchF/ppaA2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (115/91):  total (118/94)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.56e-01

Only 100 of 243 total aligned reads displayed.

AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGC  >  NC_014034/735134‑735332
                                                                                                    |                                                                                                    
 aaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcc                                                                                                     >  2:3334114/1‑101 (MQ=255)
  aaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccc                                                                                                    <  2:2565873/101‑1 (MQ=255)
       gTCTTGTCACGAGAAAGTGTCAATGAAAACTTATACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcccc..cccc                                                                                             <  1:3803469/101‑1 (MQ=255)
          ttGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCgg                                                                                          >  1:898253/1‑101 (MQ=255)
          ttGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCgg                                                                                          >  1:3700734/1‑101 (MQ=255)
           tGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGc                                                                                         >  2:2411475/1‑101 (MQ=255)
           tGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGc                                                                                         >  1:1607295/1‑101 (MQ=255)
            gTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCt                                                                                        <  1:1570552/101‑1 (MQ=255)
              cACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTaa                                                                                      >  2:220879/1‑101 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAAc                                                                                    >  1:80032/1‑101 (MQ=255)
                  agaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACcg                                                                                  >  1:835248/1‑101 (MQ=255)
                   gaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACcgc                                                                                 <  2:876026/101‑1 (MQ=255)
                   gaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACcgc                                                                                 >  1:1045499/1‑101 (MQ=255)
                    aaaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACcgcg                                                                                >  1:3503036/1‑101 (MQ=255)
                     aaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCgg                                                                               >  2:3936367/1‑101 (MQ=255)
                     aaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCCGCCAAACCGCgg                                                                               >  2:1579845/1‑101 (MQ=255)
                      aGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGt                                                                              >  2:2744411/1‑101 (MQ=255)
                      aGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGt                                                                              <  2:59139/101‑1 (MQ=255)
                      aGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCC..CCCCCGGCTAAACCGCGGTc                                                                             >  1:2991333/1‑101 (MQ=255)
                      aGTGTCAAAGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCTGt                                                                              >  2:3691694/1‑101 (MQ=255)
                       gtgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTc                                                                             <  1:1741519/101‑1 (MQ=255)
                       gtgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCAC..CCCCCGGCTAAACCGCGGTc                                                                             <  1:3288534/101‑1 (MQ=255)
                        tgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCa                                                                            >  1:1070630/1‑101 (MQ=255)
                        tgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCa                                                                            <  2:338692/101‑1 (MQ=255)
                        tgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCa                                                                            <  2:3741392/101‑1 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCAc                                                                           >  2:1296777/1‑101 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACt                                                                       >  1:4357586/1‑101 (MQ=255)
                              tGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTc                                                                      <  1:19953/101‑1 (MQ=255)
                              ngANANNTTACACGCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTc                                                                      <  1:1822380/100‑1 (MQ=255)
                               gAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCg                                                                     >  2:94930/1‑101 (MQ=255)
                                aaaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCgc                                                                    >  2:3641346/1‑100 (MQ=255)
                                aaaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGt                                                                    <  1:2612163/101‑1 (MQ=255)
                                aaaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGt                                                                    >  1:3435214/1‑101 (MQ=255)
                                 aaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTc                                                                   >  1:2345257/1‑101 (MQ=255)
                                  aaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTcc                                                                  <  1:220906/101‑1 (MQ=255)
                                  aaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCCGCTAAACCGCGGGCACCACTCGAcc                                                                  >  1:4400281/1‑101 (MQ=255)
                                   aCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCg                                                                 >  2:1012606/1‑101 (MQ=255)
                                    cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCgg                                                                >  2:451184/1‑101 (MQ=255)
                                     ttACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCggg                                                               >  1:2012637/1‑101 (MQ=255)
                                      tACACTCAGGGGAGGGACGAAAAGCCGCTGCCCTTGCGCGCAAGGGTGCCTTGATACATCTGCCAT..AACCCTGCTTAACAGCGGTCACGACTAGTACGGGc                                                              >  2:1801734/1‑101 (MQ=255)
                                       acacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCa                                                             >  2:1678330/1‑101 (MQ=255)
                                        cacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGa                                                           >  1:1441850/1‑101 (MQ=255)
                                         acTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCCCGCCTCGCCCGGGCAGa                                                           >  2:2896247/1‑101 (MQ=255)
                                          cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGCCCCGTCCGGGCAGaa                                                          >  1:3135502/1‑101 (MQ=255)
                                          cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                          >  2:113531/1‑101 (MQ=255)
                                           tCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaaa                                                         <  2:2548893/101‑1 (MQ=255)
                                           tCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACCCGTCCGGGCAGaaa                                                         >  1:1910204/1‑101 (MQ=255)
                                           tCAGGGGCGGGACCAAACGCCGCTGCCCTTGCGCGCAAGGGCTCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaaa                                                         <  2:416669/101‑1 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                       >  2:3191501/1‑101 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                       >  2:795372/1‑101 (MQ=255)
                                              ggggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcg                                                      <  2:2853852/101‑1 (MQ=255)
                                              ggggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcg                                                      <  1:3901194/101‑1 (MQ=255)
                                                  cGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcg                                                  >  1:1209660/1‑101 (MQ=255)
                                                  cGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcg                                                  >  2:1098963/1‑101 (MQ=255)
                                                   gggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCATGACTCGTCCGGGCAGAAACcgggcgg                                                 >  1:3732712/1‑101 (MQ=255)
                                                    ggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcggg                                                <  2:2320082/101‑1 (MQ=255)
                                                    ggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcggg                                                <  2:1283279/101‑1 (MQ=255)
                                                    ggACGAAACGCCGCGGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcggg                                                <  2:3292619/101‑1 (MQ=255)
                                                     gACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCgggcaggg                                               <  2:979151/101‑1 (MQ=255)
                                                       cGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCgggcggggcg                                             <  1:451224/101‑1 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                            >  1:4049611/1‑101 (MQ=255)
                                                         aaaCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcggg                                           >  2:1318644/1‑101 (MQ=255)
                                                          accgccggTTCCCTTGCTTGCAAGGGCGCCTTGATGCGGCGGCCCC..CCCCCGGCTAAACCGGGGTCACGGCGTGTCCGGGCAGAAACCGGGCGGGGCGGGa                                          <  2:1990220/99‑1 (MQ=255)
                                                             gccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgc                                       <  1:565969/101‑1 (MQ=255)
                                                               cgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgcgc                                     <  1:3307449/101‑1 (MQ=255)
                                                                gcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCACCCCCGGCTAAACCGCGGTCACGACTCGCCCGGGCCGAAACCGGGCGGGGCGGGGTgcg                                      >  1:4244924/1‑101 (MQ=255)
                                                                gcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGcc                                    >  1:2409226/1‑101 (MQ=255)
                                                                gcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGcc                                    <  1:3179463/101‑1 (MQ=255)
                                                                 cTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGGGCGCCt                                   >  1:3359656/1‑101 (MQ=255)
                                                                 cTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCt                                   >  2:2655523/1‑101 (MQ=255)
                                                                      cgtGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtct                              <  2:4362569/99‑1 (MQ=255)
                                                                       ttGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTtctc                             >  2:440909/1‑101 (MQ=255)
                                                                            cgcAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCt                        <  1:3209929/101‑1 (MQ=255)
                                                                             gcAGGGTCGCCTTGGTGCAGCGGCCCT..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGAGGCGCCTTTTCTCAACCTg                       <  2:736208/101‑1 (MQ=255)
                                                                             gcAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTg                       <  2:3135278/101‑1 (MQ=255)
                                                                              cAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTgg                      <  2:2346034/101‑1 (MQ=255)
                                                                               aaGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGc                     <  1:2879531/101‑1 (MQ=255)
                                                                               aaGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGc                     >  2:1496430/1‑101 (MQ=255)
                                                                                aGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGcc                    <  2:3027504/101‑1 (MQ=255)
                                                                                 gggCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGccc                   <  1:1175592/101‑1 (MQ=255)
                                                                                 gggCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGccc                   <  1:4295632/101‑1 (MQ=255)
                                                                                  ggggcCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCg                  <  2:1406561/98‑1 (MQ=255)
                                                                                   gcgcCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGGGCCTTTTCTCAACCCGGCCCGt                 >  1:1346845/1‑101 (MQ=255)
                                                                                    cgcCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTa                <  1:1278356/101‑1 (MQ=255)
                                                                                    cgcCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCCTa                >  1:4005659/1‑101 (MQ=255)
                                                                                       tttatgccggCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCt             <  1:736101/92‑1 (MQ=255)
                                                                                       cTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCt             <  1:3011500/101‑1 (MQ=255)
                                                                                       cTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCt             >  1:3641446/1‑101 (MQ=255)
                                                                                       cTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCt             <  1:3863232/101‑1 (MQ=255)
                                                                                        ttGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTTCTCAACCTGGCCCGTATCtt            >  1:2080886/1‑101 (MQ=255)
                                                                                        ttGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCtt            >  2:3454025/1‑101 (MQ=255)
                                                                                          gATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGc          <  1:1678441/101‑1 (MQ=255)
                                                                                           aTGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcg         <  2:2028020/101‑1 (MQ=255)
                                                                                           aTGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCCTTTCTCAACCTGGCCCCTCTCTTGcg         >  2:4437381/1‑101 (MQ=255)
                                                                                            tGCAGCGG.CCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtc       <  1:224572/101‑1 (MQ=255)
                                                                                              cAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcg      >  2:423701/1‑101 (MQ=255)
                                                                                               aGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGGCTCTTGcgtcgg     >  2:1816347/1‑100 (MQ=255)
                                                                                                gCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTg    <  1:2788346/101‑1 (MQ=255)
                                                                                                  ggCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTGGTGGc  >  2:1151907/1‑101 (MQ=255)
                                                                                                  ggCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGc  <  1:1780832/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCC..CCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGC  >  NC_014034/735134‑735332

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG