Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,003,050 +G 1.9% coding (356/738 nt) pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,003,0501.→G 1.9% 2.2 ‑2.9 ‑0.8158coding (356/738 nt)pssACDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (57/98):  total (60/98)
Fisher's exact test strand distribution p-value = 5.31e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.75e-01

Only 100 of 188 total aligned reads displayed.

GTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCGGTC  >  NC_014034/1002950‑1003150
                                                                                                     |                                                                                                        
gTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCt                                                                                                           >  1:2067106/1‑101 (MQ=255)
gTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCt                                                                                                           <  2:3341242/101‑1 (MQ=255)
    cgcCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGc.c                                                                                                     <  2:2411890/101‑1 (MQ=255)
     gcCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggc                                                                                                     >  1:4433927/1‑98 (MQ=255)
      cccGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcg                                                                                                    >  1:358323/1‑97 (MQ=255)
      cccGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcg                                                                                                    >  2:2612964/1‑97 (MQ=255)
       ccGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcgg                                                                                                   >  2:1461900/1‑96 (MQ=255)
       ccGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggcgg                                                                                                   >  2:2280969/1‑96 (MQ=255)
       ccGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggccg                                                                                                   >  2:1291463/1‑96 (MQ=255)
        cGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCggg                                                                                                 >  1:921076/1‑100 (MQ=255)
            gTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.ggggggggtgca                                                                                              >  2:4034679/1‑91 (MQ=255)
            gTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCaa                                                                                             <  1:782245/101‑1 (MQ=255)
             tGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAc                                                                                            <  2:1048334/101‑1 (MQ=255)
             tGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAc                                                                                            <  2:1635345/101‑1 (MQ=255)
              gCTCGTCTATCGCTTCGCGATGGGCTAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.g                                                                                          <  2:4426147/101‑1 (MQ=255)
              gCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCgggccgaaa                                                                                           >  1:3525945/1‑94 (MQ=255)
                tCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCgggccgaccgg                                                                                         >  1:2080110/1‑92 (MQ=255)
                tCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTg                                                                                        <  1:1046537/101‑1 (MQ=255)
                 cGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCTGGGG.CCGGTTcccgcgc                                                                                         >  1:2752166/1‑95 (MQ=255)
                  gTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCTAA.GTggc                                                                                      >  1:1290906/1‑99 (MQ=255)
                   tCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGGCAAccgggcc                                                                                      >  1:283032/1‑95 (MQ=255)
                   tCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGAcc                                                                                     <  1:47498/101‑1 (MQ=255)
                    cTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.GCGGGTCAAC.CTGAccc                                                                                    >  1:255762/1‑101 (MQ=255)
                    cTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGAccc                                                                                    <  2:2132033/101‑1 (MQ=255)
                    cTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGAccc                                                                                    <  1:3254213/101‑1 (MQ=255)
                         gCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCCACGGGGACCCgggg                                                                                >  1:3613440/1‑101 (MQ=255)
                          cTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCCGGTCGAC.GGGAGCCCGGGCg                                                                              >  2:3711625/1‑101 (MQ=255)
                          cTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCg                                                                              <  1:914602/101‑1 (MQ=255)
                           ttCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCCAC.GTGGCCCcgggggg                                                                             >  1:1250869/1‑98 (MQ=255)
                             cgcgATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCgagg                                                                           <  1:2523121/101‑1 (MQ=255)
                             cgcgATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCgagg                                                                           <  1:1529401/101‑1 (MQ=255)
                              gcgATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCTTC.GGGACCCcggggggggg                                                                          >  1:113174/1‑95 (MQ=255)
                               cgATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTTCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCgaggag                                                                         <  2:3870065/101‑1 (MQ=255)
                                gATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGaggagg                                                                        <  2:1921450/101‑1 (MQ=255)
                                  tGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGgc                                                                      <  1:423268/101‑1 (MQ=255)
                                  tGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGgc                                                                      <  1:2441050/101‑1 (MQ=255)
                                       gAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAAC.CGGACCCCGGGGGGGGGGGGGGCggg                                                                 >  2:3483268/1‑101 (MQ=255)
                                          gggAATGCTCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCCGGTCGAC.CTGACCCCGGGGGCGGAGGGGGCggggga                                                              >  1:3057824/1‑98 (MQ=255)
                                          gggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CGGGGTCAAC.GGGACCCCGGGGGGGGGGGGGGGGGGcga                                                              >  1:3423391/1‑99 (MQ=255)
                                          gggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCaa                                                              <  2:3285843/101‑1 (MQ=255)
                                          gggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCaa                                                              <  1:11564/101‑1 (MQ=255)
                                           ggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGTGAGGAGGGCGCGGGCAA.g                                                            <  2:3553691/101‑1 (MQ=255)
                                           ggAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.g                                                            <  2:2451294/101‑1 (MQ=255)
                                            gAATGGGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAAC.GCGACCCCGGGGGGGGGGGGGGGgggggaga                                                            >  1:1568865/1‑96 (MQ=255)
                                            gAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCGAC.GTGACCCCGGGCGGGGGGGGGGCggggaggg                                                            >  1:3782626/1‑96 (MQ=255)
                                             aaTGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAAA.GGGGCCCCGGGGGGGGGGGGGGCgggggagaa                                                           >  2:1567872/1‑95 (MQ=255)
                                              aTGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGCGCCGGGT.GAC.GGGGCCCCGGGGGAGCACGGCGCggggcggccc                                                          >  2:3347095/1‑94 (MQ=255)
                                               tGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAAC.GTGACCCCGGGCGGGGGGGGGGGGGGGAA.GG.ccc                                                       >  2:204014/1‑101 (MQ=255)
                                               tGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.ccc                                                       <  2:2534984/101‑1 (MQ=255)
                                               tGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.ccc                                                       <  2:3190174/101‑1 (MQ=255)
                                                gcgcTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCa                                                      <  1:2028056/101‑1 (MQ=255)
                                                gcgcTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCa                                                      <  2:3814223/101‑1 (MQ=255)
                                                   ctgggctgggTTTTCGTGCTGGTCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCCAC.CTGACCCCGGGCGGGGGGGGGGGGGGGGA.GA.ACCAttt                                                   >  1:3287985/1‑101 (MQ=255)
                                                   ctgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAAC.GCGACCCCGGGGGGGGGGGGGGCGGGGGA.GA.ccccctt                                                   >  2:2479266/1‑97 (MQ=255)
                                                   ctgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCAttt                                                   <  1:3713297/101‑1 (MQ=255)
                                                    tgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGTTTCAC.GTGACCCCGGGCGGGGGGGGGGGGGGGGA.GA.ccccattc                                                  >  2:409110/1‑96 (MQ=255)
                                                    tgggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTc                                                  <  2:743114/101‑1 (MQ=255)
                                                     ggtctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCa                                                 <  2:535351/98‑1 (MQ=255)
                                                     gggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAAC.GTGGCCCCGGGCGGGGGGGGGGCGGGGAAGGA.CCCAttta                                                  >  2:4384611/1‑100 (MQ=255)
                                                      ggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCAc                                                <  1:805293/101‑1 (MQ=255)
                                                      ggctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGGTCACC.CTGACACGGCGGGGGGGGGGGGCGGGCAC.GA.CGCAtatgac                                                >  2:4315371/1‑96 (MQ=255)
                                                       gctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGTTCAAC.GGGACCCGGGGGGGGGGGGGGGGGGGGGA.AA.ACCAtttttcc                                               >  2:2812477/1‑97 (MQ=255)
                                                        ctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCg                                              <  1:2281114/101‑1 (MQ=255)
                                                        ctgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCAGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCg                                              <  1:2957602/101‑1 (MQ=255)
                                                         tgggTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCCAC.GTGACCCGGGGGGGGGGGGGGGGGGGGAA.GA.CCCAttttccccg                                             >  2:3741645/1‑95 (MQ=255)
                                                         tgggTTTTCGAGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAAC.GGGACCCCGGGCGGGGGGGGGGGGGGGAA.GACCCCATTTCACCg                                              >  2:2246439/1‑101 (MQ=255)
                                                             ttttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCCGGTCAAC.GTGACCCCGGGCGGGGGGGGGGCGGGGGA.GA.CCCCCTTTACCCGCggt                                         >  1:4036743/1‑99 (MQ=255)
                                                             ttttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGtt                                         <  1:2747093/101‑1 (MQ=255)
                                                              tttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCCAC.GCGGACCGGGGCGGGGGGGGGGGGGGGCA.GA.CCCCATTCACCCGCGCTc                                        >  1:2159558/1‑101 (MQ=255)
                                                              tttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTc                                        <  1:3390956/101‑1 (MQ=255)
                                                              tttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTc                                        <  2:3525697/101‑1 (MQ=255)
                                                              tttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTc                                        <  1:1733312/101‑1 (MQ=255)
                                                               ttCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCCAC.GTGACCCCGGGGGGGGAGGGGGCGGGGGA.GA.ACCCCTTTACCCGCGGTcc                                       >  1:1648713/1‑101 (MQ=255)
                                                                tCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCg                                      <  2:2440831/101‑1 (MQ=255)
                                                                   tGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCa                                   <  1:2240766/101‑1 (MQ=255)
                                                                      tGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAACGGGGACCCGGGGGGGGGGGGGGGGGGGC.A.GA.CCCCTTTTACCGGCGGTCCCGCCCcg                                >  1:677264/1‑101 (MQ=255)
                                                                      tGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACcg                                <  1:713686/101‑1 (MQ=255)
                                                                        aTCTATGCCGCCTCGGCCTGTCTGCGGCT.GGG.CCGGGTCCAC.GTGGCCCCGGGGGGGGGGGGGGCGGGGCA.GA.CCCCTCTCAACCGCGTTCCCGCACcggc                              >  2:2732380/1‑101 (MQ=255)
                                                                          ttATACCGCCTCGGCCTGTCTGCTGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCCAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACcggcgg                            <  2:2555182/100‑1 (MQ=255)
                                                                          cTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACcggcgg                            <  1:1462006/101‑1 (MQ=255)
                                                                           tATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCggg                           <  1:1778831/101‑1 (MQ=255)
                                                                            aTGCCGCCTCGGCCTGTCTTCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCgggg                          <  1:3994880/101‑1 (MQ=255)
                                                                             tGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCCGTTCAAC.GTGGCCCGGGGCGAGGAGGGCGTGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCggggg                         <  2:732237/101‑1 (MQ=255)
                                                                              gccgccTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGc                        <  2:564515/101‑1 (MQ=255)
                                                                              gccgccTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGc                        <  2:4206423/101‑1 (MQ=255)
                                                                                cgccTCGGCCTGTCTGCGGCT.GGG.CCGGGTCAAC.ATGACCCCGGGCGAGGAGGGGGCGGGGAA.GA.CCCATTTTACCGGCGTTCCCGCCACGGGgggggggg                      >  1:2815176/1‑98 (MQ=255)
                                                                                 gcgtCGGCCTGTCTGCGGCT.GGC.CAGGTTCAAC.GTGCCCCGGGACGAGGAGGGCGCGGGCAA.GA.CCCATTTCTCCGGCGTTCCGGCACGGGCGGGGGCGAt                     <  2:2936508/98‑1 (MQ=255)
                                                                                   cTCGGCCTGTCTGCGGCT.GGC.CCGGGTCAAC.GTGACCCGGGGGGGGGAGGGGGCGGGGAA.GA.CCCATTTTACCCGCGTTCCGGCACCGGCgggggggggtt                   >  2:917266/1‑95 (MQ=255)
                                                                                       gCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGgc               <  1:1567977/101‑1 (MQ=255)
                                                                                       gCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGgc               <  1:1021599/101‑1 (MQ=255)
                                                                                             cTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCgctgct         <  2:1699143/101‑1 (MQ=255)
                                                                                               gCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCCCCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCtgctgc       <  1:2504609/101‑1 (MQ=255)
                                                                                               gCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGAGATTCTGGCGCtgctgc       <  1:409150/101‑1 (MQ=255)
                                                                                                cGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGcc      <  2:1547278/101‑1 (MQ=255)
                                                                                                  gCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGGGCTGCTGCCgg    >  2:1208828/1‑101 (MQ=255)
                                                                                                   cT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGGTTCTGGGGCTGCTGCCgg  >  1:4042549/1‑100 (MQ=255)
                                                                                                    t.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGGGATTCTGGCGCTGCTGCCGGTc  >  1:267226/1‑101 (MQ=255)
                                                                                                    t.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTGTGGCGCCGCTGcccgcc  >  2:2841878/1‑97 (MQ=255)
                                                                                                    t.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCgggc  >  1:4134532/1‑99 (MQ=255)
                                                                                                    t.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCCGCTGCCgggc  >  1:2193970/1‑99 (MQ=255)
                                                                                                     |                                                                                                        
GTCGCGCCCGGGGTGCTCGTCTATCGCTTCGCGATGGGCGAGGGGAATGCGCTGGGCTGGGTTTTCGTGCTGATCTATGCCGCCTCGGCCTGTCTGCGGCT.GGC.CCGGTTCAAC.GTGACCCGGGGCGAGGAGGGCGCGGGCAA.GA.CCCATTTCACCGGCGTTCCGGCACCGGCGGGGGCGATTCTGGCGCTGCTGCCGGTC  >  NC_014034/1002950‑1003150

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG