Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,200,794 +G 100% intergenic (+3/‑114) trpF → / → trpB1 phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,200,7941.→G100.0% 124.3 174intergenic (+3/‑114)trpF/trpB1phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta
Reads supporting (aligned to +/- strand):  new base (29/107):  ref base (27/11):  total (56/118)
Fisher's exact test strand distribution p-value = 2.35e-08
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-06

Only 100 of 201 total aligned reads displayed.

GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTG  >  NC_014034/1200694‑1200888
                                                                                                        |                                                                                              
  gcggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA...GGGG.gg                                                                                              >  1:297932/1‑101 (MQ=255)
   cggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.ggg                                                                                             <  2:3786147/101‑1 (MQ=255)
   cggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA...GGGG.ggg                                                                                             >  1:1244496/1‑101 (MQ=255)
    ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGGGCAGGGGTA...GGGG.gggg                                                                                            >  1:868508/1‑101 (MQ=255)
      cTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gggggt                                                                                          <  2:3024980/101‑3 (MQ=255)
       tCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGgtt                                                                                         <  2:86344/101‑1 (MQ=255)
        cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA...GGGC.ggggggtt                                                                                        >  2:2865338/1‑97 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGgtttc                                                                                       <  1:3709473/101‑3 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGgtttc                                                                                       <  1:3865959/101‑3 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGgtttc                                                                                       <  1:2282129/101‑3 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGgtttc                                                                                       <  1:2137061/101‑3 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGgtttc                                                                                       <  2:2493852/101‑3 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA...GGGG.ggggggttc                                                                                       >  1:2519856/1‑96 (MQ=255)
          ggTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTca                                                                                       <  2:3576843/101‑1 (MQ=255)
          ggTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggggttca                                                                                      >  1:3253978/1‑95 (MQ=255)
          ggTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.gggggggtct                                                                                      >  2:3950112/1‑95 (MQ=255)
             cGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcaccc                                                                                    <  2:2378343/101‑1 (MQ=255)
              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccc                                                                                   <  2:3483908/101‑1 (MQ=255)
              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccc                                                                                   <  2:2225807/101‑1 (MQ=255)
              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.gggggggggccacc                                                                                  >  1:1596135/1‑91 (MQ=255)
              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGGCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGAG..GGGG.GGGGGTTcacccc                                                                                   >  2:154757/1‑101 (MQ=255)
               aCGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcaccccc                                                                                  <  2:576300/101‑1 (MQ=255)
               aCGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGGTTcaccccc                                                                                  >  2:2118888/1‑101 (MQ=255)
                 gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTcaccccccc                                                                                >  1:557556/1‑99 (MQ=255)
                 gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccccac                                                                                <  1:2672569/101‑1 (MQ=255)
                  tgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccccaca                                                                               <  2:3718858/101‑1 (MQ=255)
                   gtcgtcGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccccacac                                                                              <  2:2077483/101‑1 (MQ=255)
                    tcgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccccacacc                                                                             <  2:1744191/101‑1 (MQ=255)
                    tcgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccccacacc                                                                             <  1:1065694/101‑1 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTcacccccccaccc                                                                            >  1:3520199/1‑101 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccccacaccc                                                                            <  1:4322885/101‑1 (MQ=255)
                       tcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccccacaccccc                                                                          <  1:1669065/101‑1 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTcacccccacaccccca                                                                         <  2:843735/101‑1 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTCACCCCCCCACCCCCCGc                                                                       >  1:622449/1‑101 (MQ=255)
                              tGGAATCGGCGCCCGGGGTCAAGGAGGCCTCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTAt                                                                   <  1:1852464/101‑1 (MQ=255)
                              tGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTAt                                                                   <  2:3914022/101‑1 (MQ=255)
                               ggAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTAtt                                                                  <  2:3519920/101‑1 (MQ=255)
                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCCCCCCCACACCCCCAGCGTATTTTg                                                               <  1:3089066/101‑1 (MQ=255)
                                   tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTgg                                                              <  2:1777677/101‑1 (MQ=255)
                                   tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTgg                                                              <  2:1013399/101‑1 (MQ=255)
                                     ggCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGcc                                                            <  2:778512/101‑1 (MQ=255)
                                     ggCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGGTTCACCCCCCCACCCCCCGCGTATTTTGGcc                                                            >  1:1751187/1‑101 (MQ=255)
                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaag                                                         <  2:411256/101‑1 (MQ=255)
                                         cccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTCACCCCCCCACCCCCAGCGTATTTTTGGCaaga                                                        >  1:4160224/1‑101 (MQ=255)
                                          ccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGGTTCACCCCCACACCCCCAGCGTATTTTGGCCaagaa                                                       >  1:3690556/1‑101 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGG.GGGG.GGGGTTTACCCCCCCCCCCCCCCCCGCATTTTGGCCaagaa                                                       >  2:4081480/1‑101 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCGaagaag                                                      >  1:733075/1‑101 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCaagaag                                                      <  1:3564292/101‑1 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGGTTCACCCCCCCACCCCCCGCGTATTTTGGCCaagaaa                                                      >  2:1153090/1‑100 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGGTTCACCCCCCCACCCCCCGCGGATTTTTGCCcagaaa                                                      >  1:4050242/1‑100 (MQ=255)
                                               gTCAAGGAGGCTGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAAc                                                  <  1:2495132/101‑1 (MQ=255)
                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTCACCCCCACCCCCCCAGCGGATTTTTGGCAAGGAAGAACAg                                                >  2:3408253/1‑101 (MQ=255)
                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTCACCCCCACACCCCCAGCGTATTTTTGCCAAGAAGAAAAAgg                                               >  2:3400448/1‑101 (MQ=255)
                                                   aggaggCCGCACGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGGTTCACCCCCACACCCCCCGCGTATTTTGGCCAAGAAGAAACAGGa                                              >  2:280102/1‑101 (MQ=255)
                                                    ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGaa                                             <  1:1998053/101‑1 (MQ=255)
                                                       ggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACtt                                          >  2:2901715/1‑101 (MQ=255)
                                                        gccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACttt                                         <  1:2639709/101‑1 (MQ=255)
                                                        gccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACttt                                         <  1:280912/101‑1 (MQ=255)
                                                          cgccCGGATTGCCTCCTTTGTCGCTGCGGCGCAGGGGTA...GGGTGGGGGTTTCCCCCCCCCACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAc                                       <  1:1130885/101‑1 (MQ=255)
                                                          cgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAc                                       <  1:4081740/101‑1 (MQ=255)
                                                          cgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAc                                       <  2:2426666/101‑1 (MQ=255)
                                                             ccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTTACCCCCACACCCCCAGCGGATTTTGGCCAAGAAGAAACAGGAACTTTAcccc                                    >  1:1939039/1‑101 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCg                                   <  2:2397240/101‑1 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCg                                   <  1:914396/101‑1 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCg                                   <  2:1684008/101‑1 (MQ=255)
                                                               ggATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGa                                  >  1:1420593/1‑101 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGTTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAg                                 <  1:2865546/101‑1 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAg                                 <  1:883211/101‑1 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAg                                 >  2:432610/1‑101 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAg                                 <  2:2821174/101‑1 (MQ=255)
                                                                  ttGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAggg                               <  2:3255274/101‑1 (MQ=255)
                                                                   tGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGGGG.GGGGTTTCCCCCCCCCCCCCCCCCCCCTTTTTGCCCCA.AAGAAAAAAAAACTTTAccccccggg                               >  1:2369033/1‑96 (MQ=255)
                                                                      cgccTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAggggggg                           <  2:2782755/101‑1 (MQ=255)
                                                                       gccTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTTACCCCCCCACCCCCAGCGTATTTTGGCCAAGAAGAAACAAGAACTTTAACCCCAgggggggg                          >  2:3513586/1‑101 (MQ=255)
                                                                       gccTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCCCCCCCACCCCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAgggggggg                          <  1:3950366/101‑1 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTTACCCCCCCACCCCCCGCGGATTTTGGCCAAGAAGAAACAGGAACTTTACCCCCAgggggggggg                        >  1:3349917/1‑99 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgcg                        <  2:1900019/101‑1 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCAAACCCCCAGGGCAATTTGGGCAAGAAGCAACAAGGACTTTACCCCGGGGGGGGGgcc                        >  1:3414711/1‑100 (MQ=255)
                                                                          tttGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAggggggggggg                       >  2:4266269/1‑98 (MQ=255)
                                                                           ttGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGTGGGGgcgcg                      <  2:253628/101‑1 (MQ=255)
                                                                            tGTCGCGGCGGCGCAGGGGTAGGGGGGG.GGGGTTTCCCCCCCCCCCCCCCCCCGTTTTTTTTCCAAAAAAAAAAAAAAAATTTCCCCCCGggggggggggg                       >  2:3187001/1‑98 (MQ=255)
                                                                            tGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGGTTTACCCCCACACCCCCCGCGGATTTTTGCCCAAAAGAAAAAAGGACCTTACCCCCAggggggggggggc                     >  1:1203440/1‑96 (MQ=255)
                                                                             gTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCt                    <  2:3199477/101‑1 (MQ=255)
                                                                             gTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCt                    <  2:1244403/101‑1 (MQ=255)
                                                                             gTCGCGGCGGCGCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCt                    <  2:26105/101‑1 (MQ=255)
                                                                              tCGCGGCGGCGCAGGGGTA...GGGTGGGGGGTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTa                   >  1:2106344/1‑101 (MQ=255)
                                                                                gcggcggcgCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATc                 >  1:583998/1‑101 (MQ=255)
                                                                                gcggcggcgCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATc                 <  1:2380271/101‑1 (MQ=255)
                                                                                 cggcggcgCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATct                <  1:4206258/101‑1 (MQ=255)
                                                                                  ggcggcgCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg               <  1:192614/101‑1 (MQ=255)
                                                                                  ggcggcgCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg               <  2:1938910/101‑1 (MQ=255)
                                                                                  gccggcgCAGGGGTAG..GGGG.GGGGTTGCCCCCCCACCCCCCCAGCGTATTTTGGCCAAGAACAACCAGAACCTTTTCCCCAAGGGGGGGGCGCGCTATctg               <  2:4391643/99‑1 (MQ=255)
                                                                                    gggcgCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctgct             <  2:2037866/100‑1 (MQ=255)
                                                                                    cggcgCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctgct             <  2:463465/101‑1 (MQ=255)
                                                                                    cggcgCAGGGGTA...GGGTGGGGGGTTCACCCCCACACCCCCCGCGGATTTTGGCCAAGAAGAAACAGGAAATTTTCCCCGAGGGGGGGGCGCGCCATctgct             >  2:1817181/1‑101 (MQ=255)
                                                                                     ggcgCAGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctgctg            <  2:3865776/101‑1 (MQ=255)
                                                                                          aGGGGTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGgc       <  1:4428007/101‑1 (MQ=255)
                                                                                           ggggTA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGgcg      <  2:3179340/101‑1 (MQ=255)
                                                                                               tA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGGCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCCCTATCTGCTGGTGGCGCGTg  >  2:1805862/1‑101 (MQ=255)
                                                                                               tA...GGGTGGGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTg  <  1:345925/101‑1 (MQ=255)
                                                                                                        |                                                                                              
GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGTTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTG  >  NC_014034/1200694‑1200888

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG