Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,212,551 +G 1.7% intergenic (+15/‑398) gltX1 → / → gcvT1 glutamyl‑tRNA synthetase/glycine cleavage T protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,212,5511.→G 1.7% 2.7 ‑3.4 ‑0.0180intergenic (+15/‑398)gltX1/gcvT1glutamyl‑tRNA synthetase/glycine cleavage T protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (55/122):  total (58/122)
Fisher's exact test strand distribution p-value = 3.23e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.13e-01

Only 100 of 194 total aligned reads displayed.

TGCGCAAGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTCCGAAGGTGTCG  >  NC_014034/1212451‑1212651
                                                                                                     |                                                                                                    
tGCGCAAGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGaa                                                                                                       <  1:3561435/101‑1 (MQ=255)
   gcAAGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.ggg                                                                                                   >  1:4344762/1‑100 (MQ=255)
   gcAAGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGc                                                                                                   <  2:594119/101‑1 (MQ=255)
    cAAGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCt                                                                                                  <  1:2406922/101‑1 (MQ=255)
     aaGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.gatcg                                                                                                 >  2:460140/1‑97 (MQ=255)
     aaGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTg                                                                                                 <  2:1224426/101‑1 (MQ=255)
      aGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTgg                                                                                                >  1:2036306/1‑101 (MQ=255)
       ggCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTggg                                                                                               <  1:1070369/101‑1 (MQ=255)
        gcgcTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTgggg                                                                                              <  2:4214524/101‑1 (MQ=255)
        gcgcTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTgggg                                                                                              <  1:217519/101‑1 (MQ=255)
          gcTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGgc                                                                                            >  2:2609925/1‑101 (MQ=255)
          gcTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGgc                                                                                            <  1:2748417/101‑1 (MQ=255)
          gcTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGgc                                                                                            <  2:2296027/101‑1 (MQ=255)
          gcTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGgc                                                                                            <  1:3150874/101‑1 (MQ=255)
            tGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCGgggggggg                                                                                          >  2:115885/1‑98 (MQ=255)
             gACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGGAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAAGGGCTgggggggg                                                                                          >  1:3763998/1‑98 (MQ=255)
              aCGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTg                                                                                        <  2:2328083/101‑1 (MQ=255)
                 gggCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGccc                                                                                     <  2:2720025/101‑1 (MQ=255)
                 gggCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGccc                                                                                     <  1:133037/101‑1 (MQ=255)
                  ggCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCCGcccc                                                                                    >  2:2127582/1‑101 (MQ=255)
                   gCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGccccc                                                                                   <  2:2042617/101‑1 (MQ=255)
                   gCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCCGGGGGGGCGGccccc                                                                                   >  1:3567552/1‑101 (MQ=255)
                     aGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGGTGGGGGGGGGGccccccc                                                                                 >  1:3227886/1‑99 (MQ=255)
                     aGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGGGGTGgcccccg                                                                                 >  1:3424183/1‑99 (MQ=255)
                     aGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGGGGTGCCCCCAg                                                                                 >  2:2477548/1‑101 (MQ=255)
                     aGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAg                                                                                 <  1:3666988/101‑1 (MQ=255)
                      agcgcATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGCCACTGCCCCCAGa                                                                                <  2:1892654/100‑1 (MQ=255)
                       gcgcATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGAc                                                                               <  2:669058/101‑1 (MQ=255)
                        cgcATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGAcc                                                                              <  2:1381072/101‑1 (MQ=255)
                         gcATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGAccc                                                                             >  2:2925355/1‑101 (MQ=255)
                          cATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCCGGGGGCGCTGGCCCCCGAcccc                                                                            >  2:1276120/1‑101 (MQ=255)
                           aTGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGGGGGCCCCCGAccccc                                                                           >  2:2104501/1‑101 (MQ=255)
                           aTGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGAccccc                                                                           <  1:4301794/101‑1 (MQ=255)
                           aTGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCGGGGGGGGCGGCCCCCCCAccccc                                                                           >  1:4115389/1‑101 (MQ=255)
                            tGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCg                                                                          <  1:2118346/101‑1 (MQ=255)
                                cccGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGat                                                                      <  2:914410/101‑1 (MQ=255)
                                cccGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGat                                                                      <  1:2594031/101‑1 (MQ=255)
                                 ccGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGata                                                                     <  2:2174370/101‑1 (MQ=255)
                                 ccGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCCGGata                                                                     >  2:2889473/1‑101 (MQ=255)
                                  cGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGGGGGGGCCCCCGACCCCCcgggtgt                                                                    >  2:4329477/1‑97 (MQ=255)
                                  cGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGGCCCCCGGCCCCCCGGatct                                                                    >  2:1339437/1‑99 (MQ=255)
                                  cGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGatat                                                                    <  2:2528214/101‑1 (MQ=255)
                                    agaTGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATAttt                                                                  <  2:3996332/101‑1 (MQ=255)
                                     gaTGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTtg                                                                 <  1:1189564/101‑1 (MQ=255)
                                      aTGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTtgt                                                                <  2:3159585/101‑1 (MQ=255)
                                        ggCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCCGACCCCCTGGAGATTTGTgg                                                              >  2:1921764/1‑101 (MQ=255)
                                         gCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGc                                                             <  2:957774/101‑1 (MQ=255)
                                          ccGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCa                                                            <  2:2395270/101‑1 (MQ=255)
                                           cGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGGTGGGGGGGGTGGCCCCCGACCCCCCGGGTAttttggggca                                                           >  1:4226195/1‑94 (MQ=255)
                                            gATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAAGGGAGGGGGGGGGGGCCCCCCACCCCCCGGGAATTTTTTGGCaa                                                           >  1:2936197/1‑101 (MQ=255)
                                            gATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGGGCGGCCCCCCGACCCCCCGGGTATTTGTGGGAAg                                                          >  2:4376239/1‑101 (MQ=255)
                                            gATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCCGGGGGGGCTGCCCCCCGACCCCCCGGGGATTTGTGGCCAg                                                          >  1:1476931/1‑101 (MQ=255)
                                             aTCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGGGGTGCCCCCCGACCCCCGGGGTATTTGTGGCAAGa                                                         >  1:1347389/1‑101 (MQ=255)
                                              tCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGAt                                                        <  1:2663515/101‑1 (MQ=255)
                                               cTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCCGGGGGGGGCGGCCCCCCACCCCCCGGGGATTTTTgggcagggg                                                       >  1:4429127/1‑94 (MQ=255)
                                                tGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGa                                                      <  1:711097/101‑1 (MQ=255)
                                                 gATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGaa                                                     <  1:1114161/101‑1 (MQ=255)
                                                  aTGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGaaa                                                    <  1:80600/101‑1 (MQ=255)
                                                       gCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGcc                                               <  2:1109262/101‑1 (MQ=255)
                                                          gATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCcgcg                                            <  2:3763745/101‑1 (MQ=255)
                                                           aTGCAGAAGGTGCTCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCgg                                           <  1:3832242/101‑1 (MQ=255)
                                                           aTGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCgg                                           <  1:3988193/101‑1 (MQ=255)
                                                              cAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGtt                                        <  2:801580/101‑1 (MQ=255)
                                                               aGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGGGCTGCCCCCAGACCCCCGGGGTATTTGTGGCAAGATGAAAGGGCCGCGGGgtt                                       >  1:575961/1‑101 (MQ=255)
                                                               aGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGttt                                       <  2:95022/101‑1 (MQ=255)
                                                               aGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGttt                                       <  1:2455500/101‑1 (MQ=255)
                                                                gAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGtttt                                      <  2:1828446/101‑1 (MQ=255)
                                                                gAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGtttt                                      <  2:14412/101‑1 (MQ=255)
                                                                 aaGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTc                                     <  2:2630282/101‑1 (MQ=255)
                                                                 aaGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTc                                     <  1:2613804/101‑1 (MQ=255)
                                                                 aaGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTc                                     <  1:4199203/101‑1 (MQ=255)
                                                                    gTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCgg                                  <  1:1921893/101‑1 (MQ=255)
                                                                    gTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCgg                                  <  2:1631598/101‑1 (MQ=255)
                                                                       cgcgcCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGccc                               <  1:4139210/101‑1 (MQ=255)
                                                                         cgcCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCtt                             <  2:1943452/101‑1 (MQ=255)
                                                                           ccAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCtttt                           <  2:3717573/101‑1 (MQ=255)
                                                                           ccAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCtttt                           <  2:3428788/101‑1 (MQ=255)
                                                                           ccAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCtttt                           <  2:3224030/101‑1 (MQ=255)
                                                                           ccAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCtttt                           <  1:2998846/101‑1 (MQ=255)
                                                                           ccAAGGCGTGACGGCTGGGGGCTGAA.GGCCGGGGGGGCTGCCCCCCGACCCCCCCGGTATTTTTTGCCAGATGAAAAGGCCGCGGGGTTTTTGGCCCcttt                           >  2:304799/1‑101 (MQ=255)
                                                                            cAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGGTTTCCGGCCCttttt                          >  2:3530364/1‑101 (MQ=255)
                                                                            cAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGGTTTCCGGCCCttttt                          >  2:2783992/1‑101 (MQ=255)
                                                                             aaGGCGGGACGGGGGGGGGCTGAA.GGCTGGGGGCGGTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCtttttt                         <  1:1777048/101‑1 (MQ=37)
                                                                              aGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGGTTTCCGGCCCTTTTTTc                        >  2:2186264/1‑101 (MQ=255)
                                                                               ggCGTGACGGCTGGGGGCTGAA.GGCTGGGGGGGGTGCCCCCCGACCCCCCGGGTATTTTTGGCAAGATGAAAAGGGCGCGGGGTTTCCGGCCCCTTTTTct                       >  2:3603589/1‑100 (MQ=255)
                                                                                  gTGACGGCGGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACACCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGt                    <  2:2552701/101‑1 (MQ=255)
                                                                                    gACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTcc                  <  1:1736201/101‑1 (MQ=255)
                                                                                    gACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTcc                  <  1:2524359/101‑1 (MQ=255)
                                                                                    gACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTcc                  <  1:2889681/101‑1 (MQ=255)
                                                                                     aCGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCg                 <  2:1520945/101‑1 (MQ=255)
                                                                                        gCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGt              <  2:3665491/101‑1 (MQ=255)
                                                                                          tGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTcc            <  2:3399339/101‑1 (MQ=255)
                                                                                          tGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTcc            <  1:1039472/101‑1 (MQ=255)
                                                                                           gggggCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTCCg           <  2:4335201/101‑1 (MQ=255)
                                                                                           gggggCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTCCg           <  2:3887867/101‑1 (MQ=255)
                                                                                             gggCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTCCGaa         <  2:2658123/101‑1 (MQ=255)
                                                                                                 tGAA.GGCTGGGGGCGCTGCCCCCCGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGGTTTCCGGCCCTTTTTTCATGTTCGGGGCCGCAGgtg     >  2:490505/1‑101 (MQ=25)
                                                                                                  gAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTCCGAAGgtgt    <  1:981797/101‑1 (MQ=255)
                                                                                                    a.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTCCGAAGGTGTCg  <  1:2370183/101‑1 (MQ=255)
                                                                                                    a.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGGTTTCCGGCCCTTTTTTCATGTCCGGGTCCGGAGGTGTCg  >  2:4113398/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
TGCGCAAGGCGCTGACGGGGCAGGCGCATGGGCCCGAGATGGCCGATCTGATGCCGCTGATGCAGAAGGTGCGCGCCAAGGCGTGACGGCTGGGGGCTGAA.GGCTGGGGGCGCTGCCCCCAGACCCCCGGGATATTTGTGGCAAGATGAAAGGGCCGCGGGTTTTCCGGCCCTTTTTTCATGTCCGGGTCCGAAGGTGTCG  >  NC_014034/1212451‑1212651

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG