Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,255,868	+C	100%	intergenic (+38/+204)	RCAP_rcc01184 → / ← RCAP_rcc01185	CsbD family protein/methyl‑accepting chemotaxis sensory transducer

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,255,868	1	.→C	100.0%	169.7	73	intergenic (+38/+204)	RCAP_rcc01184/RCAP_rcc01185	CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (39/34):  ref base (0/0):  total (39/34)

AAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGG....CTG...TCTCGGTCCGCCCGAAGAGGGGGGGCGCTGTGGTAC..CCCTGCGGGACTCCCCCCGGGATATTTCCGCCA  >  NC_014034/1255795‑1255937                                                                               |                                                                          aagaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAgggg                                                   >  1:1365122/1‑101 (MQ=255) aagaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAgggg                                                   >  1:1861851/1‑101 (MQ=255)  agaagGAAGTCGACGACTTCCTGTCGAACCTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAggggg                                                  <  1:1531825/101‑1 (MQ=255)   gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAgggggg                                                 <  1:3493453/101‑1 (MQ=255)   gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAgggggg                                                 >  2:2508018/1‑101 (MQ=255)   gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAgggggg                                                 >  2:3744508/1‑101 (MQ=255)   gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAgggggg                                                 <  1:1138366/101‑1 (MQ=255)    aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAggggggg                                                <  2:4196118/101‑1 (MQ=255)    aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAggggggg                                                >  2:3667716/1‑101 (MQ=255)    aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAggggggg                                                >  1:2744945/1‑101 (MQ=255)    aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAggggggg                                                >  1:3745402/1‑101 (MQ=255)    aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGGCCGCCCGAAGAggggggg                                                >  2:1507428/1‑101 (MQ=255)     aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAgggggggg                                               >  2:4427589/1‑100 (MQ=255)     aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAgggggggg                                               >  2:3951213/1‑100 (MQ=255)     aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGgc                                               <  1:1553248/101‑1 (MQ=255)     aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGgc                                               >  2:630571/1‑101 (MQ=255)     aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGgc                                               >  2:638444/1‑101 (MQ=255)     aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGgc                                               <  2:2499847/101‑1 (MQ=255)     aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGgc                                               <  1:4025076/101‑1 (MQ=255)       gaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGgggc                                             >  1:757970/1‑101 (MQ=255)       gaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGCTCCGCCCGAAGAGGGGGGgcgc                                             >  2:187901/1‑101 (MQ=255)        catctgaccacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCt                                            <  1:881123/96‑1 (MQ=255)        aagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCt                                            <  2:1861727/101‑1 (MQ=255)        aagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCt                                            >  2:2737685/1‑101 (MQ=255)         agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtg                                           >  1:3882123/1‑101 (MQ=255)           tCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgcc                                         >  2:2150188/1‑99 (MQ=255)           tCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgcc                                         <  2:1466701/101‑3 (MQ=255)            cgacgaCTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccc                                        <  1:1812151/101‑4 (MQ=255)            cgacgaCTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccc                                        >  1:394430/1‑98 (MQ=255)             gacgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGTAGAGGGGGGGCGCtagatc                                       >  2:881038/1‑96 (MQ=255)             gacgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgcccc                                       <  1:864945/101‑5 (MQ=255)             gacgaaTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgcccc                                       >  2:1981089/1‑97 (MQ=255)              acgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccc                                      >  2:3062607/1‑96 (MQ=255)               cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                     <  2:2997192/101‑7 (MQ=255)                gacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGgggcggccccccc                                    >  1:1426699/1‑92 (MQ=255)                gacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccc                                    >  1:2351587/1‑94 (MQ=255)                 acTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgt                                   <  2:3748421/101‑9 (MQ=255)                 acTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgt                                   >  1:3934407/1‑93 (MQ=255)                 acTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgt                                   <  1:3342418/101‑9 (MQ=255)                  cTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtc                                  >  1:702763/1‑92 (MQ=255)                  cTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtc                                  >  1:3496864/1‑92 (MQ=255)                   ttCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtcc                                 >  2:964146/1‑91 (MQ=255)                                  tGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTccccc                  >  2:2482499/1‑101 (MQ=9)                                   gACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                 >  2:1214859/1‑101 (MQ=9)                                   gACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                 >  2:4023558/1‑101 (MQ=9)                                    aCCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCg                >  2:1741696/1‑101 (MQ=9)                                     ccTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCgg               <  2:2266284/101‑1 (MQ=2)                                     ccTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCgg               <  2:4124869/101‑1 (MQ=2)                                     ccTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCgg               <  2:277196/101‑1 (MQ=2)                                      cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg              <  2:1347009/101‑1 (MQ=2)                                       tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa             <  1:4032098/101‑1 (MQ=2)                                       tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa             >  2:4260507/1‑101 (MQ=2)                                       tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGC..CCGCGCTTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa             <  2:911090/101‑1 (MQ=2)                                        aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGCCTCCCCCCGGGat            >  2:2089905/1‑101 (MQ=2)                                        aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat            >  2:633933/1‑101 (MQ=2)                                        aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat            <  2:374610/101‑1 (MQ=2)                                        aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat            >  2:486652/1‑101 (MQ=2)                                          gggTGCCGGACCGGACCCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGatat          <  1:4227425/101‑1 (MQ=0)                                          gggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGGCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGatat          <  2:2966379/101‑1 (MQ=0)                                          gggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGatat          <  1:473656/101‑1 (MQ=2)                                            gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAttt        <  2:2422724/101‑1 (MQ=0)                                            gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAttt        <  1:1481920/101‑1 (MQ=0)                                            gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAttt        >  1:2077205/1‑101 (MQ=0)                                            gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAttt        >  2:2587775/1‑101 (MQ=0)                                            gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAttt        <  2:3015245/101‑1 (MQ=0)                                             tGCCGGCCCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTc       <  2:1934760/101‑1 (MQ=2)                                             ggCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTc       <  2:3523985/100‑1 (MQ=2)                                              gCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTcc      <  2:1711321/101‑1 (MQ=2)                                                cggaccggaACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGc    <  2:508957/101‑1 (MQ=2)                                                cggaccggaACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGc    <  2:1495320/101‑1 (MQ=2)                                                cggaccggaACCGGGGGCCTTCGGGACCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGc    >  1:2699348/1‑101 (MQ=2)                                                 ggaccggaACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGcc   <  2:1943684/101‑1 (MQ=2)                                                  gaccggaACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa  <  2:1426592/101‑1 (MQ=2)                                                  gaccggaACCGGGGGCCTTCGGGCCCCCGC..TTTCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa  >  1:2367910/1‑101 (MQ=2)                                                                               |                                                                          AAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGG....CTG...TCTCGGTCCGCCCGAAGAGGGGGGGCGCTGTGGTAC..CCCTGCGGGACTCCCCCCGGGATATTTCCGCCA  >  NC_014034/1255795‑1255937 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG