Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,827 +C 2.8% intergenic (+14/+143) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8271.→C 2.8% 11.2 0.0 ‑0.2216intergenic (+14/+143)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (83/127):  total (85/131)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.55e-01

Only 100 of 231 total aligned reads displayed.

TATGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG  >  NC_014034/1303727‑1303919
                                                                                                     |                                                                                                 
tATGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTg                                                                                                    <  2:4254389/101‑1 (MQ=255)
 aTGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..c                                                                                                 <  2:1763218/101‑1 (MQ=255)
 aTGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..c                                                                                                 <  2:2152237/101‑1 (MQ=255)
    tgCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..cccc                                                                                              <  1:1107735/101‑3 (MQ=255)
     gCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..c                                                                                           >  2:2706048/1‑101 (MQ=255)
      cTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..cc                                                                                          >  2:3462454/1‑100 (MQ=255)
       tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctc                                                                                         <  1:2550358/101‑3 (MQ=255)
       tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctc                                                                                         <  1:216499/101‑3 (MQ=255)
       tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctc                                                                                         <  1:1883077/101‑3 (MQ=255)
        cAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGGGCTG..CCCc..ctcg                                                                                        >  1:471466/1‑98 (MQ=255)
        cAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctcg                                                                                        >  2:3713942/1‑98 (MQ=255)
         aaaCGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctcgg                                                                                       <  1:1711297/101‑5 (MQ=255)
               ggcgcgtTAGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ccccgtttcgc                                                                                 >  2:137241/1‑91 (MQ=255)
               ggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGc                                                                                   >  1:4133363/1‑101 (MQ=255)
                 cgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCt                                                                                 >  2:1083553/1‑101 (MQ=255)
                  gcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTc                                                                                <  2:2723289/101‑1 (MQ=255)
                    ggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAc                                                                              >  2:157184/1‑101 (MQ=255)
                    ggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAc                                                                              <  1:770578/101‑1 (MQ=255)
                     gTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGGTGGCCCCC..CGGCTTCGCCTCCcc                                                                             >  2:2546351/1‑101 (MQ=255)
                      tGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCccc                                                                            >  1:2676906/1‑101 (MQ=255)
                      tGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAccc                                                                            <  2:2082976/101‑1 (MQ=255)
                       ggCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAcccc                                                                           <  1:3724255/101‑1 (MQ=255)
                        gCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAccccc                                                                          <  1:1026270/101‑1 (MQ=39)
                         cGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCg                                                                         <  1:2083569/101‑1 (MQ=39)
                         cGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCg                                                                         >  2:1217857/1‑101 (MQ=39)
                         cGATGGAGGGCACGAAGGACAGCCTCTCCCCCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGCCGCTG..CCCCCTCGGCTTCGCCTCACCCCCg                                                                         <  1:3971448/101‑1 (MQ=255)
                          gATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCgg                                                                        >  2:1868296/1‑101 (MQ=39)
                           aTGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGGTG..CCCCCTCGGCTTCGCCTCATCCCCggg                                                                       >  2:1291626/1‑101 (MQ=255)
                           aTGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCggg                                                                       <  2:518430/101‑1 (MQ=39)
                             ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                     <  2:622757/101‑1 (MQ=38)
                              gAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGata                                                                    <  1:391459/101‑1 (MQ=38)
                                   cACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgg                                                               <  2:471414/101‑1 (MQ=38)
                                    aCGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTggg                                                              <  1:3415657/101‑1 (MQ=38)
                                     cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                             <  1:1447642/101‑1 (MQ=38)
                                     cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGCTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                             <  1:3739743/101‑1 (MQ=37)
                                      gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCCCCCCGGGATATTTGGGGc                                                            >  2:3730921/1‑101 (MQ=38)
                                      gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                            >  1:537155/1‑101 (MQ=38)
                                      gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                            <  1:3376921/101‑1 (MQ=38)
                                       aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTGCCCCCC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                           >  2:1483551/1‑101 (MQ=38)
                                       aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                           <  2:2105852/101‑1 (MQ=38)
                                           aCAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGGTATTTGGGGCAAGAt                                                       >  2:2677245/1‑101 (MQ=37)
                                            cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.a                                                     <  1:1058716/101‑1 (MQ=37)
                                            cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.a                                                     <  2:775306/101‑1 (MQ=37)
                                             aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGGGGTGCCCCCC..CGGCTTCGCCTCCCCCCCCGGGTATTTGGGGGAAGAT.aa                                                    >  1:1552880/1‑101 (MQ=255)
                                             aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aa                                                    >  2:1963024/1‑101 (MQ=37)
                                                cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc.c                                                <  1:909789/101‑1 (MQ=37)
                                                cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGGTATTTGGGGCAAGAT.AAAc.c                                                >  2:3936609/1‑101 (MQ=37)
                                                  gTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc.ccc                                              >  1:675918/1‑101 (MQ=37)
                                                   tCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCCGCCCCCC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCa                                             >  1:3584021/1‑101 (MQ=37)
                                                    cccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCTCCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCAt                                            <  2:3617591/101‑1 (MQ=37)
                                                     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                           <  1:2455505/101‑1 (MQ=37)
                                                     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                           <  1:2504877/101‑1 (MQ=37)
                                                     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                           >  1:2298181/1‑101 (MQ=37)
                                                     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                           <  1:2094341/101‑1 (MQ=37)
                                                     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                           <  1:297474/101‑1 (MQ=37)
                                                       gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                         >  2:2713562/1‑101 (MQ=37)
                                                       gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                         <  1:1209386/101‑1 (MQ=37)
                                                        cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCtt                                        <  2:2294799/101‑1 (MQ=37)
                                                        cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCGCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCCtt                                        >  2:2516216/1‑101 (MQ=25)
                                                         gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                       >  2:549019/1‑101 (MQ=37)
                                                         gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                       >  2:954734/1‑101 (MQ=37)
                                                          aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                      <  2:1655431/101‑1 (MQ=37)
                                                          aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                      >  1:1964968/1‑101 (MQ=37)
                                                           aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCa                                     <  1:2941568/101‑1 (MQ=37)
                                                           aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCa                                     <  1:560586/101‑1 (MQ=37)
                                                            aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAc                                    <  1:4067251/101‑1 (MQ=37)
                                                             cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAcc                                   <  1:2909380/101‑1 (MQ=37)
                                                               cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCtt                                 <  2:1157380/101‑1 (MQ=37)
                                                                 gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGc                               <  2:232175/101‑1 (MQ=37)
                                                                  cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGcc                              <  2:709830/101‑1 (MQ=37)
                                                                   gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                             >  1:381672/1‑101 (MQ=37)
                                                                      ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCCTTTCCCCTTGCCGCaa                          >  1:2350921/1‑101 (MQ=25)
                                                                       cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                         <  1:3252659/101‑1 (MQ=37)
                                                                       cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                         >  2:676889/1‑101 (MQ=37)
                                                                        gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAat                        >  1:945980/1‑101 (MQ=25)
                                                                          ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCTCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                      <  1:4408920/101‑1 (MQ=37)
                                                                           gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                     <  2:2894150/101‑1 (MQ=37)
                                                                           gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                     <  2:4366074/101‑1 (MQ=37)
                                                                            tCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATcc                    <  2:3109187/101‑1 (MQ=37)
                                                                                cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                >  2:3450970/1‑101 (MQ=25)
                                                                                cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                <  2:1935375/101‑1 (MQ=37)
                                                                                cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGAGATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                >  1:3764368/1‑101 (MQ=37)
                                                                                 gTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg               >  2:1044292/1‑101 (MQ=37)
                                                                                 gTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg               <  1:2713752/101‑1 (MQ=37)
                                                                                   ttAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggggg             >  2:3877198/1‑101 (MQ=25)
                                                                                     cggacggggggCTCTGC.CCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAAt        <  1:3652129/95‑1 (MQ=2)
                                                                                     aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg           >  1:2803911/1‑101 (MQ=37)
                                                                                     aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg           >  2:3034117/1‑101 (MQ=25)
                                                                                       gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGaa         >  1:3093844/1‑101 (MQ=25)
                                                                                        cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt        <  2:3835025/101‑1 (MQ=37)
                                                                                        cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt        <  1:2281954/101‑1 (MQ=37)
                                                                                         aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCGCCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       <  1:2705808/101‑1 (MQ=25)
                                                                                         aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCACCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       <  1:1772363/101‑1 (MQ=25)
                                                                                          aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg      <  2:3443337/101‑1 (MQ=37)
                                                                                          aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAGTg      >  2:3748250/1‑101 (MQ=25)
                                                                                           gggggCTCTGC.CCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca  <  2:4436892/101‑2 (MQ=2)
                                                                                           gggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg     <  2:337839/101‑1 (MQ=37)
                                                                                            ggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgc    >  2:2568582/1‑101 (MQ=37)
                                                                                             gggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc   >  1:423240/1‑101 (MQ=37)
                                                                                              ggCGCTG..CCCCCGCGGCTTCGCGACACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTGGCCGCAAATGTCCCGGGGGGTGAATTGgccg  <  2:4177995/101‑1 (MQ=25)
                                                                                                     |                                                                                                 
TATGTGCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG  >  NC_014034/1303727‑1303919

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG