Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,303,933	+G	100%	intergenic (+120/+37)	urtE → / ← RCAP_rcc01231	urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,303,933	1	.→G	100.0%	189.6	92	intergenic (+120/+37)	urtE/RCAP_rcc01231	urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (65/27):  ref base (0/0):  total (65/27)

TTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCATCAGGGTCTTGCGCACCGGCTTGAACGCATAAAGC  >  NC_014034/1303856‑1304030                                                                                         |                                                                                                   tttGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcg                                                                                   <  1:954819/101‑1 (MQ=14) tttGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcg                                                                                   >  1:3534568/1‑101 (MQ=14)  ttGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcgg                                                                                  <  2:4304026/101‑1 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGTGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcggg                                                                                 >  2:300071/1‑101 (MQ=9)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcggg                                                                                 <  2:3221761/101‑1 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcggg                                                                                 >  2:2885445/1‑101 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcggg                                                                                 >  2:3787186/1‑101 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcggg                                                                                 <  2:1970132/101‑1 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcggg                                                                                 >  1:3138648/1‑101 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGgggcggg                                                                                 >  2:3016145/1‑101 (MQ=9)     gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggcc                                                                               >  2:806173/1‑101 (MQ=14)     gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggcc                                                                               <  2:4133103/101‑1 (MQ=14)     gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggcc                                                                               >  1:3736375/1‑101 (MQ=14)     gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggcc                                                                               >  2:4154139/1‑101 (MQ=14)      ggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccg                                                                              >  2:3622874/1‑101 (MQ=14)        cAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgca                                                                            >  2:3888972/1‑101 (MQ=14)        cAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgca                                                                            >  2:1556678/1‑101 (MQ=14)        cAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgca                                                                            >  1:3356076/1‑101 (MQ=14)         aaGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcag                                                                           >  1:777618/1‑101 (MQ=14)         aaGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcag                                                                           >  1:210835/1‑101 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagg                                                                          >  2:1255868/1‑101 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagg                                                                          <  1:203533/101‑1 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagg                                                                          >  1:2649734/1‑101 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagg                                                                          >  2:2472281/1‑101 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagg                                                                          >  2:3254270/1‑101 (MQ=14)           gATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcaggg                                                                         >  2:4423962/1‑101 (MQ=14)            aTAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggc                                                                        >  1:93677/1‑101 (MQ=14)             tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggcc                                                                       >  2:4247280/1‑101 (MQ=14)             tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggcc                                                                       <  2:2480909/101‑1 (MQ=14)              aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccggagggccc                                                                      >  2:2690958/1‑100 (MQ=9)              aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggccg                                                                      >  2:423736/1‑101 (MQ=14)              aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggccg                                                                      >  1:1427834/1‑101 (MQ=14)              aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggccg                                                                      >  2:560068/1‑101 (MQ=14)               aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggccgc                                                                     >  2:1781408/1‑101 (MQ=14)               aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggccgc                                                                     >  1:3966317/1‑101 (MQ=14)                aCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCgggccgcagggccgca                                                                    >  1:3863071/1‑101 (MQ=14)                 ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCAc                                                                   >  2:2303029/1‑101 (MQ=14)                 ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCAc                                                                   <  2:3813664/101‑1 (MQ=14)                 ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCAc                                                                   >  1:1227406/1‑101 (MQ=14)                  cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCAcc                                                                  >  1:486319/1‑101 (MQ=14)                  cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCAcc                                                                  >  1:2702403/1‑101 (MQ=14)                   ccATCCTGTCACCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTG.CGGGGCGGGCCGCAGGGCCGCAccc                                                                 <  1:4417613/101‑1 (MQ=9)                    cctCCTTTCACCTTGCCGCAAATTTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCt                                                                <  2:4031089/99‑1 (MQ=9)                    cATCCTTTCACCTTGCCTTAAATATCCCGGGGGGTGAACTGGCC....GAAGACCAAGAGGGGGGCAGCGCCCCTTTTT.CGGGGGGGGCCGCAGGGCCGCACCCt                                                                <  2:2638958/101‑1 (MQ=9)                    cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCt                                                                <  1:549071/101‑1 (MQ=14)                    cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCt                                                                >  2:263089/1‑101 (MQ=14)                      tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGt                                                              >  1:453942/1‑101 (MQ=14)                      tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGt                                                              >  2:1007778/1‑101 (MQ=14)                       ccTTTCGCCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTc                                                             <  2:4102175/101‑1 (MQ=9)                       ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTc                                                             >  2:2882358/1‑101 (MQ=14)                       ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTc                                                             >  1:2939892/1‑101 (MQ=14)                       ccTTTCACCATGCCGCAAATATCCCGGGGGGTGAATTAGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGACGCCGGGCCGCCCCCTGTc                                                             >  2:437568/1‑101 (MQ=2)                        cTTTCACCTTGCCGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCa                                                            <  2:4261764/101‑1 (MQ=9)                        cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCa                                                            >  2:4376887/1‑101 (MQ=14)                        cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCa                                                            <  2:1670313/101‑1 (MQ=14)                        cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCa                                                            >  1:288626/1‑101 (MQ=14)                          ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                                                          >  2:2490301/1‑101 (MQ=14)                          ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                                                          >  2:1114489/1‑101 (MQ=14)                          ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                                                          >  1:1608606/1‑101 (MQ=14)                          tcccccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                                                          <  2:3460298/97‑1 (MQ=9)                           tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                                                         <  1:3716282/101‑1 (MQ=14)                           tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                                                         >  1:2494327/1‑101 (MQ=14)                            cACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcgc                                                        <  2:3630601/101‑1 (MQ=17)                             aCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCa                                                       >  1:4357470/1‑101 (MQ=17)                              ccTTGCGGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                                                      <  2:3837152/101‑1 (MQ=12)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                                                      <  1:2706238/101‑1 (MQ=17)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                                                      <  1:1962743/101‑1 (MQ=17)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                                                      >  1:4227772/1‑101 (MQ=17)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                                                      <  1:4175414/101‑1 (MQ=17)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                                                      <  1:3936863/101‑1 (MQ=17)                               cTTGCCGCAAATCTCGCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                                                     <  1:2009981/101‑1 (MQ=12)                               cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCCGCGCCTc                                                     >  2:665847/1‑101 (MQ=9)                               cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                                                     >  1:1216211/1‑101 (MQ=17)                               cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                                                     >  1:2659833/1‑101 (MQ=17)                                ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc                                                    >  2:2808500/1‑101 (MQ=17)                                ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc                                                    <  2:2193020/101‑1 (MQ=17)                                 tGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATccc                                                   >  1:300442/1‑101 (MQ=17)                                 tGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATccc                                                   >  1:2732783/1‑101 (MQ=17)                                    cGCAAATATCCCGGGGGGTGAATTGGGCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa                                                <  1:2639677/101‑1 (MQ=12)                                    cGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa                                                >  1:2053348/1‑101 (MQ=17)                                      cAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc                                              >  1:667094/1‑101 (MQ=17)                                      cAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGAAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc                                              >  1:3807763/1‑101 (MQ=17)                                       aaaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc                                             >  2:3635357/1‑101 (MQ=17)                                       aaaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc                                             >  2:833017/1‑101 (MQ=17)                                       aaaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc                                             >  1:1603506/1‑101 (MQ=17)                                        aaTCTCCCGGGGTGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCCCCCTGTCAGCGCATCCCCAAgccc                                            <  2:667874/101‑1 (MQ=12)                                        aaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc                                            >  2:907262/1‑101 (MQ=17)                                        aaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc                                            >  1:520147/1‑101 (MQ=17)                                         atatCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccg                                           >  2:4287746/1‑101 (MQ=17)                                           atCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAACCCCCAgcccgcc                                         >  1:3085756/1‑101 (MQ=12)                                            tCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc                                        >  1:2698747/1‑101 (MQ=18)                                            tCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc                                        >  1:85357/1‑101 (MQ=18)                                             cccGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGcccgcccg                                       >  1:2348261/1‑101 (MQ=18)                                                ggggggTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCAt                                    <  2:3093620/101‑1 (MQ=18)                                                                                  gcccccc.cccctTTTTGCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCATCAGGGTCTTGCGCACCGGCTTGAACGCATa      <  1:3254502/95‑1 (MQ=255)                                                                                     cccc.cccctTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCATCAGGGTCTTGCGCACCGGCTTGAACGCATAAAgc  <  1:560120/98‑1 (MQ=255)                                                                                         |                                                                                                   TTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTT.CGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCATCAGGGTCTTGCGCACCGGCTTGAACGCATAAAGC  >  NC_014034/1303856‑1304030 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG