Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,320,651 +G 100% intergenic (+109/+76) potI1 → / ← RCAP_rcc01247 polyamine ABC transporter permease PotI/membrane transport family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,320,6511.→G100.0% 513.0 164intergenic (+109/+76)potI1/RCAP_rcc01247polyamine ABC transporter permease PotI/membrane transport family protein
Reads supporting (aligned to +/- strand):  new base (85/78):  ref base (0/0):  total (86/78)

Only 100 of 178 total aligned reads displayed.

GATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.A..GG.CA..GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGG.GC.AGCGCCCCCTTGCTTACGGCAGGATC  >  NC_014034/1320555‑1320739
                                                                                                      |                                                                                            
 aTCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CA..gggg                                                                                         >  1:2990819/1‑100 (MQ=255)
    cGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AT.GG.CA..ggggctg                                                                                      <  2:3374597/101‑5 (MQ=255)
         cGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGgctgcgctg                                                                                  <  1:828963/101‑1 (MQ=37)
           ccgAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGcc                                                                                <  2:1422130/99‑1 (MQ=25)
           cAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTTACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGcc                                                                                >  1:1233595/1‑101 (MQ=25)
           cAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGcc                                                                                >  2:615934/1‑101 (MQ=25)
            aGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGccc                                                                               >  1:3426861/1‑101 (MQ=25)
            aGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGccc                                                                               <  1:2610035/101‑1 (MQ=37)
              aaaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCtt                                                                             <  1:1150666/101‑1 (MQ=37)
               aaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTc                                                                            <  1:2032336/101‑1 (MQ=37)
               aaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTc                                                                            <  2:2967643/101‑1 (MQ=37)
               aaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCCAGGATATTTGAAGCAAGGGGGAAATG.AA.AAGGG.CG..GGGGTGCGCGGCCCTTc                                                                            >  1:3303217/1‑101 (MQ=25)
                 ggggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCAt                                                                          <  1:770344/101‑1 (MQ=37)
                  gggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATc                                                                         <  2:1479898/101‑1 (MQ=37)
                   ggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCt                                                                        >  2:3483778/1‑101 (MQ=38)
                   ggcgCTGTCTTCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATGTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCt                                                                        <  1:1870025/98‑1 (MQ=37)
                       cTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGcc                                                                    >  2:2838018/1‑101 (MQ=38)
                        tGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCca                                                                   >  2:1725715/1‑101 (MQ=38)
                        tGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCca                                                                   >  2:2483492/1‑101 (MQ=38)
                         gTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGcccc                                                                  >  2:2001321/1‑99 (MQ=38)
                          tCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCcaca                                                                 <  1:2213965/101‑1 (MQ=38)
                          nnnnnnnnnntctTTTTNTGCTCAAAATTCCCCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCcaca                                                                 <  2:2024313/91‑1 (MQ=25)
                           cTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACaa                                                                >  2:4101662/1‑101 (MQ=38)
                           cTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACaa                                                                >  2:1127077/1‑101 (MQ=38)
                            tGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGCAGCAAGGTGAAACTG.AA.A..GG.GAAGGGGCTGCGCTGCCCTTCATCTTGcccaaaa                                                               >  2:4436974/1‑96 (MQ=25)
                            tGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACaaa                                                               <  2:1090270/101‑1 (MQ=38)
                            tGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCCAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCCTCTTGCcacaca                                                               >  1:527858/1‑99 (MQ=37)
                             gCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAat                                                              <  1:1868168/101‑1 (MQ=38)
                             gCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAAATG.AA.AG.GG.CAG.GGGCTGCACTGCCCTTCATCTTGCCACaaaa                                                              >  1:2307802/1‑100 (MQ=37)
                              ccccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAata                                                             >  1:228514/1‑101 (MQ=38)
                               cccgcTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAatat                                                            >  2:54960/1‑101 (MQ=38)
                               cccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAatat                                                            <  2:3248700/101‑1 (MQ=38)
                               cccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAatat                                                            <  1:70160/101‑1 (MQ=38)
                                 cccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATcc                                                          <  2:3208525/101‑1 (MQ=38)
                                  ccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATccc                                                         >  1:106470/1‑101 (MQ=39)
                                    tctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgg                                                       <  2:3289595/101‑1 (MQ=38)
                                     ctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCggg                                                      >  1:2372427/1‑101 (MQ=39)
                                      ttttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgggg                                                     <  1:2468159/101‑1 (MQ=38)
                                      ttttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgggg                                                     >  2:2847416/1‑101 (MQ=38)
                                       tttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCggggg                                                    >  2:184415/1‑101 (MQ=39)
                                       tttGCTGCGCAAAATTCAAACCCCAAGGAAATTTGAAGCAAGGTGAAACTG.AA.A..GGACAG.GGGATGTGCTGCCCTTAAGCTTGCCACAAATATCGCggggg                                                    >  2:2101953/1‑101 (MQ=255)
                                        ttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgggggg                                                   <  2:395998/101‑1 (MQ=38)
                                        ttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgggggg                                                   <  2:479783/101‑1 (MQ=38)
                                         tgctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGa                                                  <  2:4113506/101‑1 (MQ=38)
                                         tgctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGa                                                  >  2:3894021/1‑101 (MQ=38)
                                           ctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAgc                                                >  1:1315747/1‑100 (MQ=38)
                                           ctgcACAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGt                                                <  2:1280721/101‑1 (MQ=38)
                                            tgcGCAAAATTCACCCCGCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGGCACAAATATCCCGGGGGGAGTc                                               >  2:729396/1‑101 (MQ=38)
                                            tgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTc                                               >  1:1740672/1‑101 (MQ=38)
                                             gcgcAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTcc                                              >  2:170663/1‑101 (MQ=38)
                                             gcgcAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTcc                                              >  2:108158/1‑101 (MQ=38)
                                                 aaaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCa                                          <  1:1700088/101‑1 (MQ=37)
                                                    aTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAggg                                       >  2:1937555/1‑101 (MQ=37)
                                                    aTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAggg                                       >  2:3822389/1‑101 (MQ=37)
                                                    aTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAggg                                       >  1:602501/1‑101 (MQ=37)
                                                     ttCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGa                                      >  1:4259232/1‑101 (MQ=37)
                                                      tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                     <  1:511294/101‑1 (MQ=37)
                                                      tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTCCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                     <  1:1541965/101‑1 (MQ=25)
                                                       cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                   >  1:3046118/1‑101 (MQ=25)
                                                        aCCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gg                                  >  1:1433210/1‑101 (MQ=25)
                                                         ccccccGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggg                                 >  2:1234960/1‑101 (MQ=25)
                                                          cccccGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg                                >  2:647849/1‑101 (MQ=25)
                                                           ccccGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.g                              <  1:1955533/101‑1 (MQ=25)
                                                              cGAGGATATTTGAAGCAAGGTGAAACTG.AAGG..GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.ggca                           >  1:3966954/1‑98 (MQ=21)
                                                              cGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.ggcg                           >  2:4258679/1‑98 (MQ=21)
                                                              cGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.ggca                           >  2:1018245/1‑98 (MQ=21)
                                                              cGAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.ggca                           <  1:1372629/101‑4 (MQ=21)
                                                               gAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.ggcag                          >  1:51842/1‑97 (MQ=21)
                                                               gAGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.ggcag                          >  1:2793633/1‑97 (MQ=21)
                                                                aGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.ggcagc                         >  2:1105812/1‑96 (MQ=21)
                                                                aGGATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg.ggcagc                         <  1:2955585/101‑6 (MQ=21)
                                                                 ggATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.Agcg                        <  2:2460303/101‑1 (MQ=18)
                                                                  gATATTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.Agcgc                       >  1:2523724/1‑101 (MQ=18)
                                                                   atatTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGcc                      <  1:3894275/101‑1 (MQ=18)
                                                                   atatTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGcc                      >  2:2526298/1‑101 (MQ=18)
                                                                    tatTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGccc                     <  1:15227/101‑1 (MQ=18)
                                                                    tatTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGccc                     >  2:2702446/1‑101 (MQ=18)
                                                                     atTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGATTCCCGCAGGGACGGGGG.GC.AGCGccgc                    >  2:3003979/1‑99 (MQ=18)
                                                                     atTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGACCCGCAGGGACGGGGG.GC.AGCGcccc                    >  1:3494476/1‑101 (MQ=18)
                                                                     atTTGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGCGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGcccc                    >  1:3794518/1‑101 (MQ=18)
                                                                      tttGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGccccc                   >  2:233644/1‑101 (MQ=18)
                                                                       ttGAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCt                  <  1:634462/101‑1 (MQ=18)
                                                                         gAAGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTg                >  2:1204319/1‑101 (MQ=18)
                                                                          aaGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGc               <  1:2838225/101‑1 (MQ=18)
                                                                          aaGCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGc               >  2:3996224/1‑101 (MQ=18)
                                                                          aaGCAAGGGGAAACTG.AA.AG.GG.CAG.GGGCTGGCCTGCCCTTCCTGTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGCC.GGGGTGC.AGGGCCCCCTTGc               >  2:3502684/1‑101 (MQ=12)
                                                                            gCAAGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCtt             <  1:2649352/101‑1 (MQ=18)
                                                                              aaGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTAc           <  1:4376370/101‑1 (MQ=18)
                                                                               aGGTGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACg          >  1:145725/1‑101 (MQ=18)
                                                                                  tGAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCa       <  2:4133990/101‑1 (MQ=18)
                                                                                  tGAAACTC.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCa       <  2:437922/101‑1 (MQ=17)
                                                                                   gAAACTGAAA.A..GG.CAG.GGGCTGCGCTGCACTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGTAGGGAG.GGGG.GCAAGCGCCCCATTGCTTACGCCAg      >  2:1968631/1‑101 (MQ=17)
                                                                                   gAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCAg      >  2:77364/1‑101 (MQ=18)
                                                                                   gAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCAg      >  2:291950/1‑101 (MQ=18)
                                                                                   gAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCAg      >  1:3640345/1‑101 (MQ=18)
                                                                                   gAAACTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCAg      >  1:1329247/1‑101 (MQ=18)
                                                                                    aaaCTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCAgg     >  2:4167239/1‑101 (MQ=18)
                                                                                     aaCTG.AA.AG.GG.CAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCAGGa    >  1:4020482/1‑101 (MQ=18)
                                                                                       cTG.AA.AG.GG.CAG.GGGCGGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGCCCCGCGGGGACGGGGG.GC.AGCGCCCCTTTGCTTACGGCAGGATc  <  2:1360885/101‑1 (MQ=17)
                                                                                       cTG.AA.A..GGTCAG.GGGCTGCGCTGCCCTTCATCTTGCCAAAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGG.GC.AGCGCCCCCTTGCTTACGGCAGGATc  <  1:1020203/101‑1 (MQ=18)
                                                                                                      |                                                                                            
GATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTG.AA.A..GG.CA..GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGG.GC.AGCGCCCCCTTGCTTACGGCAGGATC  >  NC_014034/1320555‑1320739

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG