Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,562 +C 1.3% intergenic (+374/‑557) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5621.→C 1.3% 3.6 0.0 ‑0.0310intergenic (+374/‑557)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (151/155):  total (153/157)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.54e-01

Only 100 of 340 total aligned reads displayed.

AAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395462‑1395662
                                                                                                        |                                                                                                    
aaaCATCGCCGCTTTGGCGGTGGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                                       >  1:2546586/1‑101 (MQ=255)
aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                                       >  2:1981166/1‑101 (MQ=255)
    aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtc                                                                                                  <  1:2519588/101‑1 (MQ=255)
       gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtcgtc                                                                                               <  1:2799301/101‑1 (MQ=255)
        ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtcgtcg                                                                                              <  2:1186595/101‑1 (MQ=255)
        ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtcgtcg                                                                                              <  2:2726385/101‑1 (MQ=255)
            tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTcc                                                                                          >  1:2598093/1‑101 (MQ=255)
            tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCCCGGGGGCGTGGGTCGGAAAACCTCGGTGCCGTCGCTCG.T.CCAGACGG.CCC.GCTCGTCGCTcc                                                                                          >  1:3182344/1‑101 (MQ=255)
                 cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCcg                                                                                     <  2:4191464/101‑1 (MQ=255)
                      gACAGGCTGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCCCgggg                                                                                >  1:3044498/1‑101 (MQ=255)
                       aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                                               >  1:1770755/1‑101 (MQ=255)
                       aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                                               <  1:2266466/101‑1 (MQ=255)
                        cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGc                                                                              <  2:1855227/101‑1 (MQ=255)
                        cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGc                                                                              <  1:1921584/101‑1 (MQ=255)
                          ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                                            <  1:1704132/101‑1 (MQ=255)
                           gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  2:1127315/1‑101 (MQ=255)
                            agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.TGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:2096070/91‑1 (MQ=2)
                             ccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  1:1581285/92‑1 (MQ=2)
                             aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  2:4069680/10‑101 (MQ=0)
                              gggaaCGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:1303047/98‑1 (MQ=255)
                               ggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGGGGATc                                                                       >  1:3221567/8‑101 (MQ=0)
                               gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:726867/101‑1 (MQ=255)
                               ataccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.A.TCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  2:2542467/8‑101 (MQ=0)
                                gctgcgggcGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:3283593/93‑1 (MQ=2)
                                ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:828281/95‑1 (MQ=2)
                                 cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  1:916137/96‑1 (MQ=2)
                                  ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  1:3928751/5‑101 (MQ=2)
                                  gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  2:3574842/5‑101 (MQ=2)
                                  gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGGGGATCgca                                                                    >  1:847854/5‑99 (MQ=2)
                                   gtggtCGCCCCCCCCGCGGGGGCGCGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:3480137/98‑1 (MQ=2)
                                   gcggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:1618627/98‑1 (MQ=2)
                                    cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  1:2847619/99‑1 (MQ=2)
                                     ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                                 <  2:2144519/100‑1 (MQ=2)
                                     ggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.CCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                                 <  2:2891007/100‑2 (MQ=2)
                                     cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.A.TCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  2:4432800/100‑1 (MQ=2)
                                     cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaat                                                                 >  2:70497/2‑99 (MQ=2)
                                      gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactc                                                                >  2:1347244/1‑99 (MQ=2)
                                      gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                                <  2:3646860/101‑3 (MQ=2)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                               >  2:2982962/1‑101 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                               <  1:1086570/101‑1 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                               <  2:1513480/101‑3 (MQ=2)
                                       ccGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGCCCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                               <  2:123644/100‑4 (MQ=2)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                              >  1:4141306/1‑101 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                              >  1:627229/1‑101 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGAGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCTGCGGGGGCGTGGATCGAAactgcg                                                              >  2:2648061/1‑97 (MQ=2)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                                             >  1:4033531/1‑96 (MQ=2)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                             >  1:2968658/1‑97 (MQ=2)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                            >  2:2359555/1‑101 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                            <  1:3095245/101‑7 (MQ=2)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                           >  1:245244/1‑101 (MQ=255)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                           >  2:353153/1‑94 (MQ=2)
                                           cccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                           <  1:3422241/100‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                                                          <  1:1030052/101‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacaccaatgc                                                          <  2:1615327/101‑9 (MQ=2)
                                            ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                          <  1:1330008/101‑8 (MQ=2)
                                            ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                          >  1:3492231/1‑94 (MQ=2)
                                             cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                                         >  2:3099463/1‑92 (MQ=2)
                                             cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                                         <  2:3704360/101‑9 (MQ=0)
                                             cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                                         >  2:2200738/1‑92 (MQ=2)
                                              ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGGGGATCGAAactatactggct                                                        >  1:1853914/1‑91 (MQ=2)
                                                 cgcgGGGGGGTGGATCGAAACATCTCGGTGCCGTCGCCCC.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                                     <  1:4301499/101‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                                   <  1:2400753/101‑1 (MQ=255)
                                                     ggggCGTGGATCGAACCATCTTGGCGCCGTTGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCGTGCGGATTGTCa                                                 <  2:2432641/101‑1 (MQ=37)
                                                     ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                                 >  2:3388108/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                                <  1:1399564/101‑1 (MQ=255)
                                                      gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAATGTCAc                                                >  1:514874/1‑101 (MQ=255)
                                                        gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                              <  1:1880216/101‑1 (MQ=255)
                                                          gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                            >  1:1518321/1‑101 (MQ=255)
                                                          gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                            >  2:2672026/1‑101 (MQ=255)
                                                          gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                            <  2:2597922/101‑1 (MQ=255)
                                                           tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                           <  2:385895/101‑1 (MQ=255)
                                                             gATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                                         >  1:4172500/1‑101 (MQ=255)
                                                             gATCGAAACAGCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                                         <  2:168255/101‑1 (MQ=255)
                                                                cGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg                                      <  1:3164830/101‑1 (MQ=255)
                                                                 gAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                                     >  2:203211/1‑101 (MQ=255)
                                                                   aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                                   <  1:2717524/101‑1 (MQ=255)
                                                                   aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                                   <  2:3835187/101‑1 (MQ=255)
                                                                    aCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa                                  <  1:3582221/101‑1 (MQ=255)
                                                                     cctctcGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                                 <  2:3182117/99‑1 (MQ=255)
                                                                     cATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                                 <  1:4063769/101‑1 (MQ=255)
                                                                      aTCTCGGTGCCGTCGCTCG.T.CCAGCCCG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                                <  1:3735028/101‑1 (MQ=255)
                                                                         tcGGTGCCGTCTCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                             <  1:2656543/101‑1 (MQ=255)
                                                                          cGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                            <  2:1439037/101‑1 (MQ=255)
                                                                           ggTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGc                           <  1:4443810/101‑1 (MQ=255)
                                                                            gTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt                          >  1:4324375/1‑101 (MQ=255)
                                                                             tGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTc                         >  2:500610/1‑101 (MQ=255)
                                                                                  tCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc                    <  1:2051075/101‑1 (MQ=255)
                                                                                   cGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcg                   >  1:1553867/1‑101 (MQ=255)
                                                                                    gCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc                  <  1:2759657/101‑1 (MQ=255)
                                                                                      tCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgg                <  1:2208265/101‑1 (MQ=255)
                                                                                        g.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg              >  2:3989161/1‑101 (MQ=255)
                                                                                            ccAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGc            <  2:551017/101‑1 (MQ=255)
                                                                                             cAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCg           <  2:1770636/101‑1 (MQ=255)
                                                                                                ccGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg        <  2:4127835/101‑1 (MQ=255)
                                                                                                 cGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGa       >  2:1040680/1‑101 (MQ=255)
                                                                                                 cGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGa       <  2:1001218/101‑1 (MQ=255)
                                                                                                  gg.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGAt      >  2:3049938/1‑101 (MQ=255)
                                                                                                   g.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:4252547/101‑1 (MQ=255)
                                                                                                   g.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATc     >  1:1057282/1‑101 (MQ=255)
                                                                                                       t.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  1:1153184/101‑1 (MQ=255)
                                                                                                        |                                                                                                    
AAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395462‑1395662

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG