Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,138 +G 3.4% coding (20/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1381.→G 3.4% 13.9 ‑1.3 ‑0.0203coding (20/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (103/93):  total (105/98)
Fisher's exact test strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 217 total aligned reads displayed.

CCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCG  >  NC_014034/1396038‑1396238
                                                                                                     |                                                                                                      
 cccccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.a                                                                                                       <  1:261066/101‑1 (MQ=255)
  ccccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.At                                                                                                      >  1:1077595/1‑101 (MQ=255)
   cccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATc                                                                                                     <  2:1010123/101‑1 (MQ=255)
    ccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCt                                                                                                    <  1:2095483/101‑1 (MQ=255)
     cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                                   <  2:2479308/101‑1 (MQ=255)
     cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                                   <  2:925021/101‑1 (MQ=255)
     cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                                   <  1:3647764/101‑1 (MQ=255)
     cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                                   <  1:610602/101‑1 (MQ=255)
         ggggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAg                                                                                              <  1:3422478/101‑1 (MQ=255)
          gggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAgg                                                                                             <  2:3055752/101‑1 (MQ=255)
            gCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCt                                                                                           <  2:2635775/101‑1 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                          >  2:3720915/1‑101 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                          >  2:873481/1‑101 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                          <  1:377884/101‑1 (MQ=255)
             cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                          <  2:2943950/101‑1 (MQ=255)
               tGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgc                                                                                        >  1:534464/1‑101 (MQ=255)
                 gATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCgcgc                                                                                      >  1:1863847/1‑101 (MQ=255)
                  aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgcc                                                                                     <  1:701668/101‑1 (MQ=255)
                  aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgcc                                                                                     >  1:3205611/1‑101 (MQ=255)
                   tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccg                                                                                    <  2:370312/101‑1 (MQ=255)
                    cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                   >  1:4269882/1‑101 (MQ=255)
                    cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                   <  1:3882472/101‑1 (MQ=255)
                     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCcccgcc                                                                                  >  1:3050384/1‑101 (MQ=255)
                      taaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCa                                                                                 <  2:1572050/100‑1 (MQ=255)
                       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCaa                                                                                >  1:1572155/1‑100 (MQ=255)
                       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                <  2:2296904/101‑1 (MQ=255)
                       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                <  2:1421925/101‑1 (MQ=255)
                       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                <  1:1204541/101‑1 (MQ=255)
                        aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAcc                                                                               >  1:3092141/1‑100 (MQ=255)
                        aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACg                                                                               >  2:1537235/1‑101 (MQ=255)
                               cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTc                                                                       <  1:3920597/101‑1 (MQ=255)
                                aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCg                                                                      >  2:2475520/1‑101 (MQ=255)
                                 tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CCGCGTCGc                                                                     >  1:1022697/1‑101 (MQ=255)
                                 tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGc                                                                     >  2:3630665/1‑101 (MQ=255)
                                  cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCt                                                                    <  2:1055562/101‑1 (MQ=255)
                                      tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGGCACG.CTGCGTCGCTcccc                                                                >  2:470058/1‑101 (MQ=255)
                                      tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTcccc                                                                <  2:507125/101‑1 (MQ=255)
                                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCTCCACG.CTGCGTCGCTcccccc                                                              <  1:2542267/101‑1 (MQ=255)
                                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCCcccccc                                                              >  2:3084293/1‑101 (MQ=255)
                                         atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTccccccc                                                             >  2:190924/1‑100 (MQ=255)
                                         atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcg                                                             <  2:127865/101‑1 (MQ=255)
                                         atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcg                                                             <  1:3478740/101‑1 (MQ=255)
                                          tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcgc                                                            >  1:562775/1‑101 (MQ=255)
                                           atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCC.CCGCGGCCCCCCCCCcccg                                                           >  1:892226/1‑101 (MQ=25)
                                             gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggg                                                         >  1:647074/1‑101 (MQ=255)
                                              cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggg                                                        >  2:3591668/1‑101 (MQ=255)
                                               ccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggggg                                                       >  1:2857460/1‑101 (MQ=255)
                                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTg                                                   <  1:3379025/101‑1 (MQ=255)
                                                    gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  2:3899311/92‑1 (MQ=2)
                                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                  >  2:1643282/1‑101 (MQ=255)
                                                      gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACC.CTGCGTCGCTCCCCCCCCGGGGGCGGgggt                                                >  1:1716904/1‑99 (MQ=255)
                                                       tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCCTGGATc                                               >  1:727353/7‑101 (MQ=2)
                                                         ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  1:2187927/1‑101 (MQ=255)
                                                         ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCCCGGGGGCGTGGATCGa                                             >  1:845441/1‑101 (MQ=255)
                                                           cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGAttcaaa                                           >  1:3280479/1‑96 (MQ=255)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGGGGGGATCGCAAcc                                         >  1:4202618/1‑101 (MQ=255)
                                                             gTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTTTTCGCC.AACTCG.CAGGCCCGCCCCCCCACGCCTGC.CCTCTCCCTCCGGGGGGCGGTGGATGGGAAcc                                         >  2:2366674/1‑101 (MQ=9)
                                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                        <  1:4032507/101‑1 (MQ=255)
                                                              tCGCTCCCCCAGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGCGGAACGAAAccc                                        >  2:954530/1‑101 (MQ=255)
                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACCg                                       >  2:3905634/1‑101 (MQ=255)
                                                               cGCTCCCCCCGCGGGGGCGTGGACCGAAACTTTTTGTC.ATCTGG.CCTGCGCTCGCCGCCGCG.CTGCGCCACTCCCCCCGCGGGGGCGTGGagccaagaccg                                       >  2:1979134/1‑91 (MQ=21)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGc                                     <  1:190951/101‑1 (MQ=255)
                                                                   ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGa                                   >  1:1534272/1‑101 (MQ=255)
                                                                    cccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaa                                  <  1:1004755/101‑1 (MQ=255)
                                                                     ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaa                                 >  2:4298928/1‑101 (MQ=255)
                                                                      cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                                <  2:4096821/101‑1 (MQ=255)
                                                                        cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                              <  2:3358831/101‑1 (MQ=255)
                                                                        cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                              <  2:4191125/101‑1 (MQ=255)
                                                                          cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                            >  1:28233/1‑101 (MQ=255)
                                                                          cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                            >  2:174180/1‑101 (MQ=255)
                                                                           gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACCCGGGGAAAAACGGc                           >  2:4216315/1‑101 (MQ=255)
                                                                           gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGc                           >  1:1987193/1‑101 (MQ=255)
                                                                            ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                          >  1:96515/1‑101 (MQ=255)
                                                                            ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                          >  1:1380032/1‑101 (MQ=255)
                                                                             gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGGTCCCCCCGCCGGGGCGGGGACCGAAATCCGGCGGAAAACGGCCa                         >  2:399914/1‑101 (MQ=255)
                                                                             gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                         <  2:3483793/101‑1 (MQ=255)
                                                                              ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                        <  2:2622726/101‑1 (MQ=255)
                                                                               gCGTGGATCGAGACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                       <  1:1276218/101‑1 (MQ=255)
                                                                               gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                       <  1:1633590/101‑1 (MQ=255)
                                                                                cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGc                      >  2:3567995/1‑101 (MQ=255)
                                                                                 gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGcc                     >  1:2469021/1‑101 (MQ=255)
                                                                                  tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                    <  1:2366345/101‑1 (MQ=255)
                                                                                  tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                    <  2:1169106/101‑1 (MQ=255)
                                                                                   ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                   >  2:2137757/1‑101 (MQ=255)
                                                                                   ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                   <  2:1128865/101‑1 (MQ=255)
                                                                                    gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                  >  2:1592997/1‑101 (MQ=255)
                                                                                    gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCCCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                  <  2:824080/101‑1 (MQ=255)
                                                                                     aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                 >  2:1709740/1‑101 (MQ=255)
                                                                                       cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggg               <  1:470110/101‑1 (MQ=255)
                                                                                        gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgggg              >  2:649611/1‑100 (MQ=255)
                                                                                          aaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgtgt            <  2:2871921/101‑1 (MQ=255)
                                                                                           aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc           <  2:2798774/101‑1 (MQ=255)
                                                                                           aCTTTTCCTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc           <  2:2179115/101‑1 (MQ=255)
                                                                                             ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg         >  1:3509715/1‑101 (MQ=255)
                                                                                             ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCCGGGGTCTg         >  2:3201355/1‑101 (MQ=255)
                                                                                                tCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGACTCCGGGTGTCTGGCt      >  2:2990862/1‑101 (MQ=255)
                                                                                                 cGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTg     >  1:3963506/1‑101 (MQ=255)
                                                                                                  gTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTgg    <  2:3965732/101‑2 (MQ=255)
                                                                                                   tC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAAGGCCATGCCTCCGGGTGTCTGGCTGTc   >  1:782812/1‑101 (MQ=255)
                                                                                                    c.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg  >  1:2293696/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
CCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCG  >  NC_014034/1396038‑1396238

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG