Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,968 +C 5.2% coding (850/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9681.→C 5.2% 47.1 ‑2.4 ‑0.0348coding (850/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/13):  ref base (163/167):  total (168/180)
Fisher's exact test strand distribution p-value = 9.12e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.82e-01

Only 100 of 381 total aligned reads displayed.

CGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGC  >  NC_014034/1396868‑1397068
                                                                                                                |                                                                                                      
cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTg                                                                                                         >  1:1571533/1‑101 (MQ=255)
cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTg                                                                                                         <  2:2204232/101‑1 (MQ=255)
 ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTg.g                                                                                                       <  2:55069/101‑1 (MQ=255)
  gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGCGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGCg.gg                                                                                                      >  1:3764269/1‑100 (MQ=255)
      agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCt                                                                                                  <  2:842323/101‑1 (MQ=255)
      agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCt                                                                                                  <  1:1781672/101‑1 (MQ=255)
           tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTccccc                                                                                             >  2:907771/1‑101 (MQ=255)
           tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTccccc                                                                                             >  1:3333695/1‑101 (MQ=255)
               ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCcgcg                                                                                         <  2:2256627/101‑1 (MQ=255)
                   gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                                     >  1:1047280/1‑101 (MQ=255)
                         ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                                <  2:619402/91‑1 (MQ=9)
                         ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                                <  2:3592324/91‑1 (MQ=9)
                          gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                               >  1:100646/10‑101 (MQ=9)
                          gtcaacactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                               <  2:1450330/92‑1 (MQ=9)
                          cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGa                                                                               >  1:657/9‑101 (MQ=0)
                           tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                              <  1:982790/93‑1 (MQ=9)
                           cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                             <  2:2408563/101‑1 (MQ=255)
                           cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                             >  2:2124760/1‑101 (MQ=255)
                           ccccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                                <  1:2609872/94‑1 (MQ=0)
                            ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  1:2299695/94‑1 (MQ=2)
                            cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                               <  1:2967672/95‑1 (MQ=2)
                             ccggtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.AGTGCG.TCTGG.A.ACA..GGAG.GTCGCTCCCCCCGCGGGGGCGGGGATCg                                                                            >  1:1340570/7‑101 (MQ=2)
                             aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCATGGATCg                                                                            <  1:3871980/95‑1 (MQ=9)
                             aacactgTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  1:3713091/95‑1 (MQ=9)
                               ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                          >  1:4076111/4‑101 (MQ=2)
                               cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                         >  2:3522513/5‑101 (MQ=2)
                                tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CCG..CGGC.GTCGCCCCCCCCGCGGGGGCGGGGATCGaaa                                                                         >  1:3938666/3‑101 (MQ=2)
                                tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGGTCGaaa                                                                         >  2:3763312/3‑101 (MQ=2)
                                ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                        <  1:1634982/98‑1 (MQ=2)
                                actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                         >  2:1092220/4‑101 (MQ=9)
                                actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                         >  1:3241675/4‑101 (MQ=9)
                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                       <  1:835095/99‑2 (MQ=2)
                                 cggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                       <  1:1177674/99‑2 (MQ=2)
                                 aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                       >  1:4414090/1‑101 (MQ=255)
                                 aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                       >  2:2773836/1‑101 (MQ=255)
                                  tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                       >  2:2499445/2‑100 (MQ=9)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                                      >  1:2414887/2‑99 (MQ=2)
                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCCGGGGCGTGGATCGAAACac                                                                      >  2:4340290/1‑100 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                     <  1:3795696/101‑1 (MQ=255)
                                    tctgtCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                                                     <  1:512214/97‑4 (MQ=2)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                                    >  1:2326655/1‑101 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                                     <  1:3408607/101‑4 (MQ=9)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                                      <  2:2341368/101‑2 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                                                    >  1:456505/1‑97 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGAGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActcgg                                                                    >  2:694300/1‑97 (MQ=9)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                                  >  2:1767107/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCG.GCGCC.GC.AGCG.AGTGCG.TCTGG.A.ACA..GGAT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActatgc                                                                   <  1:308799/101‑6 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                                  <  1:1925187/101‑7 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                                  >  2:1983309/1‑95 (MQ=2)
                                      ccTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                                  <  2:2041455/100‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACACCa                                                                    >  2:3707640/1‑101 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                                                                 >  2:1186468/1‑101 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGTTCGAAActaccccg                                                                 >  1:4213785/1‑94 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                                 >  1:19824/1‑94 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                                 <  1:1292481/101‑8 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                                  >  2:3795821/1‑95 (MQ=9)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                                >  2:1396058/1‑101 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                                                >  2:2604205/1‑93 (MQ=2)
                                        cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                                <  1:3585781/100‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                                  <  1:2065473/101‑3 (MQ=2)
                                         ccccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccgccacg                                                                >  2:4148970/1‑93 (MQ=0)
                                          cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                               <  2:3233692/101‑10 (MQ=9)
                                          cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                               <  1:941266/101‑10 (MQ=9)
                                            cccGCGGGGGCGTGGATCCAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.tcg                                                           <  1:1688655/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                              >  2:1654479/1‑95 (MQ=0)
                                             ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcg.tcgt                                                          >  2:504291/1‑101 (MQ=255)
                                              cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGtt                                                         <  1:1837398/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTg                                                        >  1:4106072/1‑101 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTg                                                        <  1:588639/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                    <  1:2687169/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                    >  2:3873928/1‑101 (MQ=255)
                                                    ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAg                                                   <  2:2915808/101‑1 (MQ=255)
                                                     gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGc                                                  <  2:4080902/101‑1 (MQ=255)
                                                      cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                 >  2:3425690/1‑101 (MQ=255)
                                                       gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCg                                                <  2:3333458/101‑1 (MQ=255)
                                                       gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGGCAGCCg                                                >  1:743041/1‑101 (MQ=255)
                                                           aTCGAACCA.GCCT.GGGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                           <  1:2104225/101‑1 (MQ=255)
                                                             cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GAt                                         <  1:278170/101‑1 (MQ=255)
                                                             cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GAt                                         <  1:3648629/101‑1 (MQ=255)
                                                                aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                      <  2:2391997/101‑1 (MQ=255)
                                                                aaCA.GCCT.CCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                      <  1:1450923/101‑1 (MQ=255)
                                                                 accatctt.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                      <  2:3505768/94‑1 (MQ=255)
                                                                   a.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGt                                   <  2:3988085/101‑1 (MQ=255)
                                                                         ggcgcG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCt                               <  1:3134398/100‑1 (MQ=255)
                                                                           cgcg.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcc                             <  1:3520122/101‑1 (MQ=255)
                                                                             cg.GC.CGCA.TCGGCT.TC.CT.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcccc                           <  2:3950673/101‑1 (MQ=255)
                                                                             cg.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcccc                           <  2:4271283/101‑1 (MQ=255)
                                                                                    gCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCcgcg                      >  1:3984929/1‑101 (MQ=255)
                                                                                    gCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCcgcg                      <  2:4441549/101‑1 (MQ=255)
                                                                                     ga.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCgg                     <  1:4370743/100‑1 (MQ=255)
                                                                                      a.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCggg                    >  2:1514486/1‑101 (MQ=255)
                                                                                           gCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCg                <  1:2015138/101‑1 (MQ=255)
                                                                                               tC.CG.C.CTG..CGTG.GACGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCGCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTgg             <  1:2216737/101‑1 (MQ=255)
                                                                                                   g.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc          >  1:109352/1‑101 (MQ=255)
                                                                                                     c.cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCg         >  2:1879371/1‑101 (MQ=255)
                                                                                                       cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa        >  2:1319296/1‑101 (MQ=255)
                                                                                                          ccgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg         <  1:2864339/96‑1 (MQ=2)
                                                                                                          ccgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg         <  1:4286534/96‑1 (MQ=2)
                                                                                                              tG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCCCCCCCCGCGGGGGCGGGGACGGAAAcgc   >  1:1589324/1‑99 (MQ=37)
                                                                                                               g.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc  <  1:1726018/101‑1 (MQ=255)
                                                                                                                |                                                                                                      
CGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGC  >  NC_014034/1396868‑1397068

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG