BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,434,381	+G	16.3%	intergenic (‑160/‑798)	RCAP_rcc01334 ← / → RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,434,381	2	.→G	16.3%	142.4 ‑0.1 ‑0.3	263	intergenic (‑160/‑798)	RCAP_rcc01334/RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand): new base (20/23): ref base (99/121): total (119/144)
Fisher's exact test strand distribution p-value = 8.68e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.64e-01

Only 100 of 279 total aligned reads displayed.


ATGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGC  >  NC_014034/1434281‑1434479


aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                          <  1:4222685/101‑1 (MQ=255)


aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                          >  2:4069980/1‑101 (MQ=255)


aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                          >  1:720661/1‑101 (MQ=255)


      tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTGTcc                                                                                                  >  2:4293373/1‑101 (MQ=255)


      tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                  >  1:4090678/1‑101 (MQ=255)


      tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                  <  2:1988112/101‑1 (MQ=255)


      tataAGCACCGCGGGCCGTAGATCGAAACAGGCGGGTTTGGGCGGAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                  <  2:2150501/101‑1 (MQ=25)


         atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGct                                                                                               <  2:1733309/101‑1 (MQ=255)


           gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                             <  2:2459098/101‑1 (MQ=255)


           gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                             <  2:161923/101‑1 (MQ=255)


           gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                             <  2:1319412/101‑1 (MQ=255)


           gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                             >  1:4305036/1‑101 (MQ=255)


            cACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTg                                                                                            >  1:2700771/1‑101 (MQ=255)


             aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGc                                                                                           >  2:195163/1‑101 (MQ=255)


             aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGc                                                                                           >  2:1063125/1‑101 (MQ=255)


               cGCTTACCGTGGATCGAAACACGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCtt                                                                                         <  2:33527/101‑1 (MQ=255)


                gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                        <  2:2877209/101‑1 (MQ=255)


                gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                        >  1:4060108/1‑101 (MQ=255)


                 cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCa                                                                                       <  2:2395705/101‑1 (MQ=255)


                  ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCAc                                                                                      <  2:3908332/101‑1 (MQ=255)


                  ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCAc                                                                                      >  2:719398/1‑101 (MQ=255)


                  ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCAc                                                                                      <  2:2703848/101‑1 (MQ=255)


                    aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                   <  2:4388734/101‑1 (MQ=255)


                    aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                   <  2:3177324/101‑1 (MQ=255)


                    aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                   >  2:945568/1‑101 (MQ=255)


                       gTGGATCGAAGCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTg                                                                               >  1:3853154/1‑101 (MQ=255)


                       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTg                                                                               >  2:3104359/1‑101 (MQ=255)


                       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTg                                                                               <  2:3018653/101‑1 (MQ=255)


                        tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGc                                                                              <  1:4354252/101‑1 (MQ=255)


                           aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTc                                                                           <  1:3004494/101‑1 (MQ=255)


                           aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CCGCGTc                                                                           >  2:1120019/1‑101 (MQ=255)


                            tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCa                                                                          <  1:836587/101‑1 (MQ=255)


                            tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCa                                                                          <  2:167/101‑1 (MQ=255)


                            tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCa                                                                          >  2:2251232/1‑101 (MQ=255)


                             cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCaa                                                                         >  2:1929464/1‑101 (MQ=255)


                               aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAAcc                                                                       >  1:1873161/1‑101 (MQ=255)


                               aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAAcc                                                                       <  1:2228310/101‑1 (MQ=255)


                                  cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGGCAACCTT..g                                                                  >  2:3697759/1‑101 (MQ=255)


                                     gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCg                                                               >  2:58299/1‑101 (MQ=255)


                                       tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCt                                                             >  1:2935972/1‑101 (MQ=255)


                                       tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCt                                                             <  1:776547/101‑1 (MQ=255)


                                         ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTcc                                                           <  1:2384602/101‑1 (MQ=255)


                                          tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTccc                                                          <  1:1368388/101‑1 (MQ=255)


                                          tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTccc                                                          <  1:2054931/101‑1 (MQ=255)


                                                cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCcgcg                                                    <  2:35022/101‑1 (MQ=255)


                                                 ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCgg                                                   >  2:2538669/1‑101 (MQ=255)


                                                      acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCg                                              >  2:836379/1‑101 (MQ=255)


                                                      acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCg                                              <  2:1412701/101‑1 (MQ=255)


                                                       ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                             <  1:3943906/101‑1 (MQ=255)


                                                        cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                            >  2:1876360/1‑101 (MQ=255)


                                                         tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCCGGGGCGGgg                                           >  1:1651916/1‑101 (MQ=255)


                                                         ngATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGNGNGtnn                                           >  1:334270/2‑99 (MQ=255)


                                                          gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGa                                          >  1:3985240/1‑101 (MQ=255)


                                                           aTCCTGCTGGGTCGCTCCCCCGGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                         >  1:2543086/1‑101 (MQ=255)


                                                           aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                         <  1:566512/101‑1 (MQ=255)


                                                            tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                        <  1:2251377/101‑1 (MQ=255)


                                                            tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                        <  1:1848825/101‑1 (MQ=255)


                                                            tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                        <  1:1530816/101‑1 (MQ=255)


                                                             gggctatcgTCGCCCCGCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                          <  2:3494524/93‑1 (MQ=2)


                                                              ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                         >  2:3561209/8‑101 (MQ=2)


                                                               tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                     >  1:2879387/1‑101 (MQ=255)


                                                               tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                     <  2:2122263/101‑1 (MQ=255)


                                                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                        <  1:171081/95‑1 (MQ=2)


                                                                gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                    <  1:2172817/101‑1 (MQ=255)


                                                                ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                       <  1:415222/96‑1 (MQ=2)


                                                                 tatcggcGCCCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                      <  2:6921/95‑1 (MQ=2)


                                                                 tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                      >  1:1161586/5‑101 (MQ=2)


                                                                 ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                   <  1:3205421/101‑1 (MQ=255)


                                                                  atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                     >  2:92064/4‑101 (MQ=2)


                                                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                 <  2:324305/101‑1 (MQ=255)


                                                                       ctcTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                             <  2:328003/99‑1 (MQ=255)


                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                <  1:3255038/101‑4 (MQ=2)


                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                             <  1:694375/101‑1 (MQ=255)


                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                              <  1:2454522/101‑6 (MQ=2)


                                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                          <  1:633948/101‑1 (MQ=255)


                                                                           ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                         <  2:1808225/101‑1 (MQ=255)


                                                                            cccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                           <  1:3674730/101‑9 (MQ=2)


                                                                            cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                        <  1:2610500/101‑1 (MQ=255)


                                                                            cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                        >  2:3925210/1‑101 (MQ=255)


                                                                            cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                        <  2:4002554/101‑1 (MQ=255)


                                                                             ccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                          <  2:3796003/101‑10 (MQ=2)


                                                                             ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.G.C.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                        <  1:887402/101‑8 (MQ=37)


                                                                              cccGGGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                      <  2:527727/101‑1 (MQ=255)


                                                                              cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                         <  1:542129/101‑11 (MQ=0)


                                                                              cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                         >  1:475795/1‑91 (MQ=0)


                                                                              cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                      <  1:1968487/101‑1 (MQ=255)


                                                                               ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                     >  2:2927204/1‑101 (MQ=255)


                                                                                   gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                 >  2:4345552/1‑101 (MQ=255)


                                                                                   gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                 >  1:945224/1‑101 (MQ=255)


                                                                                     gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg               <  1:3460559/101‑1 (MQ=255)


                                                                                        cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa            <  1:1063245/101‑1 (MQ=255)


                                                                                         gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc           >  2:1508196/1‑101 (MQ=255)


                                                                                         gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc           <  2:1205490/101‑1 (MQ=255)


                                                                                         gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc           >  1:2618805/1‑101 (MQ=255)


                                                                                           ggATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GGCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg         <  1:1120110/101‑1 (MQ=255)


                                                                                               cGAAAC..CGCCCCGGTCTTGTTCCCG.GCCTGCGCCACCCTT..GT.GCCCCCCCCCGGGGGGGGGGGGTCGAAACCCGGATTGCATGGCGCCGAGGGACAGGCtt     <  1:1929593/101‑1 (MQ=2)


                                                                                                gAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt    >  1:3403590/1‑101 (MQ=255)


                                                                                                 aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtttc   >  1:3135624/1‑100 (MQ=255)


                                                                                                 aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg   >  1:102042/1‑101 (MQ=255)


                                                                                                  aaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc  >  2:4228079/1‑101 (MQ=255)


ATGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGC  >  NC_014034/1434281‑1434479

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG