BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,434,400	+C	16.2%	intergenic (‑179/‑779)	RCAP_rcc01334 ← / → RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,434,400	1	.→C	16.2%	138.8 ‑0.1 0.0	260	intergenic (‑179/‑779)	RCAP_rcc01334/RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand): new base (19/23): ref base (103/115): total (122/138)
Fisher's exact test strand distribution p-value = 8.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.


TACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1434300‑1434500


tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                                        >  2:3943652/1‑101 (MQ=255)


 aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                                      >  2:945568/1‑101 (MQ=255)


 aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                                      <  2:4388734/101‑1 (MQ=255)


   cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.Ct                                                                                                   >  1:4335384/1‑101 (MQ=255)


        aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CCGCGTc                                                                                              >  2:1120019/1‑101 (MQ=255)


         tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCa                                                                                             >  2:2251232/1‑101 (MQ=255)


          cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCaa                                                                                            >  2:1929464/1‑101 (MQ=255)


            aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAAcc                                                                                          >  1:2973246/1‑101 (MQ=255)


             aaCAGGCTGCTTTGGGCGGAACACCTCATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCt                                                                                         <  1:1783265/101‑1 (MQ=255)


              aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCtt                                                                                        >  2:1968358/1‑101 (MQ=255)


              aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCtt                                                                                        <  1:4157542/101‑1 (MQ=255)


               cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGGCAACCTTg                                                                                       >  2:3697759/1‑101 (MQ=255)


                aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGt                                                                                      <  1:3854351/101‑1 (MQ=255)


                  gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCg                                                                                    >  2:697192/1‑101 (MQ=255)


                  gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCg                                                                                    >  2:58299/1‑101 (MQ=255)


                    tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCt                                                                                  <  1:776547/101‑1 (MQ=255)


                      ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTcc                                                                                <  1:2384602/101‑1 (MQ=255)


                       tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTccc                                                                               <  1:2054931/101‑1 (MQ=255)


                       tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTccc                                                                               <  2:1851344/101‑1 (MQ=255)


                       tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTccc                                                                               <  1:1368388/101‑1 (MQ=255)


                        ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTcccc                                                                              >  1:837354/1‑101 (MQ=255)


                         tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTccccc                                                                             >  1:4232647/1‑101 (MQ=255)


                           gggggAACACCTGATCCTGCTGGGTCGGTCCCCCCGCGGGGGCGTGGATCGAAGC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCcg                                                                           <  1:487255/98‑1 (MQ=255)


                           ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCcg                                                                           <  2:1017068/101‑1 (MQ=255)


                             cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCcgcg                                                                         <  2:35022/101‑1 (MQ=255)


                              ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                                        >  2:3214101/1‑101 (MQ=255)


                                   acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                                   >  2:836379/1‑101 (MQ=255)


                                      tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                                <  2:1288748/101‑1 (MQ=255)


                                      tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCCGGGGCGGgg                                                                >  1:1651916/1‑101 (MQ=255)


                                       gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                               >  1:3985240/1‑101 (MQ=255)


                                       gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTAGCTCCCCCCGCGGGGGCGTGGa                                                               >  2:1412933/1‑101 (MQ=255)


                                        aTCCTGCTGGGTCGCTCCCCCGGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  1:2543086/1‑101 (MQ=255)


                                        aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  1:3725152/1‑101 (MQ=255)


                                        aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:3056009/101‑1 (MQ=255)


                                        aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  1:2914540/1‑101 (MQ=255)


                                         tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:1530816/101‑1 (MQ=255)


                                         tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:676489/101‑1 (MQ=255)


                                          gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                               >  1:3412312/9‑101 (MQ=2)


                                          gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                               >  2:1722519/9‑101 (MQ=2)


                                          gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                               >  2:4286213/9‑101 (MQ=2)


                                          gggctatcgTCGCCCCGCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  2:3494524/93‑1 (MQ=2)


                                           ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:3548482/94‑1 (MQ=2)


                                           ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  2:3561209/8‑101 (MQ=2)


                                            tgctgGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  2:2979209/1‑101 (MQ=255)


                                            gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:171081/95‑1 (MQ=2)


                                             ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  1:2200877/96‑1 (MQ=2)


                                              tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           <  1:1860024/97‑1 (MQ=2)


                                              ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                        <  1:3205421/101‑1 (MQ=255)


                                               atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  2:92064/4‑101 (MQ=2)


                                                 cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                        <  2:3394029/100‑1 (MQ=2)


                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                       <  1:248053/101‑2 (MQ=2)


                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                    <  1:928347/101‑1 (MQ=255)


                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                    >  2:2135634/1‑101 (MQ=255)


                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                    >  1:244714/1‑101 (MQ=255)


                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                                      >  1:3909243/1‑99 (MQ=2)


                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                     <  1:3255038/101‑4 (MQ=2)


                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                    <  1:2619103/101‑5 (MQ=2)


                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                    >  2:3879670/1‑97 (MQ=2)


                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                 >  1:610430/1‑101 (MQ=255)


                                                         cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                             <  1:719478/101‑1 (MQ=255)


                                                         cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                             >  1:4145621/1‑101 (MQ=255)


                                                         cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                             <  1:2610500/101‑1 (MQ=255)


                                                         cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                             <  2:1798100/101‑1 (MQ=255)


                                                         cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                             <  2:4002554/101‑1 (MQ=255)


                                                          ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                            <  1:763554/101‑1 (MQ=255)


                                                           cccGGGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                           <  2:527727/101‑1 (MQ=255)


                                                           cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                              <  1:542129/101‑11 (MQ=0)


                                                           cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAacgctgctgaca                                              >  2:3253945/1‑91 (MQ=0)


                                                           cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                           <  1:1968487/101‑1 (MQ=255)


                                                            ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                          >  1:4056004/1‑101 (MQ=255)


                                                            ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                          <  2:2512499/101‑1 (MQ=255)


                                                            ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                          >  2:2927204/1‑101 (MQ=255)


                                                              gcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                        >  1:112119/1‑101 (MQ=255)


                                                               cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                       >  2:3102553/1‑101 (MQ=255)


                                                                gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                      >  2:4345552/1‑101 (MQ=255)


                                                                     cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                 <  1:2729583/101‑1 (MQ=255)


                                                                     cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                 <  1:1063245/101‑1 (MQ=255)


                                                                     cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                 >  1:2171886/1‑101 (MQ=255)


                                                                      gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                <  1:2250696/101‑1 (MQ=255)


                                                                       tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                               <  2:1548467/101‑1 (MQ=255)


                                                                            cGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                          >  2:677295/1‑101 (MQ=255)


                                                                            cGAAAC..CGCCCCGGTCTTGTTCCCG.GCCTGCGCCACCCTTGT.GCCCCCCCCCGGGGGGGGGGGGTCGAAACCCGGATTGCATGGCGCCGAGGGACAGGCtt                          <  1:1929593/101‑1 (MQ=2)


                                                                             gAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                         >  2:3166258/1‑101 (MQ=255)


                                                                              aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtttc                        >  1:3135624/1‑100 (MQ=255)


                                                                              aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                        >  1:102042/1‑101 (MQ=255)


                                                                               aaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                       >  2:4228079/1‑101 (MQ=255)


                                                                                    gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                    >  1:3355969/1‑101 (MQ=255)


                                                                                    gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                    <  2:3298033/101‑1 (MQ=255)


                                                                                    gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTACCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTCCAGt                    >  2:2637283/1‑101 (MQ=255)


                                                                                     tctcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGCATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                   >  1:3304569/1‑101 (MQ=255)


                                                                                       tcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc                 >  1:1628774/1‑101 (MQ=255)


                                                                                        ccGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCt                <  2:2474479/101‑1 (MQ=255)


                                                                                          gCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc              >  2:3746879/1‑101 (MQ=255)


                                                                                          gCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc              <  1:1264735/101‑1 (MQ=255)


                                                                                          gCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc              <  1:4378314/101‑1 (MQ=255)


                                                                                             ctGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc           <  2:2366743/101‑1 (MQ=255)


                                                                                              tGCTTCACG.G.CTGCGTCAATCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc          <  2:2063317/101‑1 (MQ=255)


                                                                                                cTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc        >  1:2068314/1‑101 (MQ=255)


                                                                                                cTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc        >  2:2876943/1‑101 (MQ=255)


                                                                                                  tCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg      >  2:2963690/1‑101 (MQ=255)


TACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1434300‑1434500

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG