Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,177 +C 100% intergenic (‑105/+120) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1771.→C100.0% 28.9 198intergenic (‑105/+120)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (71/113):  ref base (8/4):  total (81/117)
Fisher's exact test strand distribution p-value = 7.02e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.57e-03

Only 100 of 217 total aligned reads displayed.

CTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCC.TTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGC  >  NC_014034/1696076‑1696276
                                                                                                        |                                                                                                   
ctcctcCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.c                                                                                                    <  2:2735726/101‑1 (MQ=255)
 tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.cc                                                                                                   <  2:3017995/101‑2 (MQ=255)
  cctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.cca                                                                                                  <  1:874580/101‑3 (MQ=255)
  cctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.cca                                                                                                  <  1:3517932/101‑3 (MQ=255)
   ctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.ccaa                                                                                                 <  2:1891847/101‑4 (MQ=255)
   ctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.ccaa                                                                                                 >  2:3599075/1‑98 (MQ=255)
     cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTGTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.ccaatt                                                                                               <  2:340517/101‑6 (MQ=255)
      ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTc                                                                                               <  1:2138323/101‑1 (MQ=255)
       cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTca                                                                                              <  1:322824/101‑2 (MQ=255)
        aaaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTcac                                                                                             >  1:2181279/1‑99 (MQ=255)
          aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCG.TTTCG.GCCAATTcgccc                                                                                           >  2:1407532/1‑97 (MQ=255)
          aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAccc                                                                                          >  1:204179/1‑101 (MQ=255)
          aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAccc                                                                                          >  2:309171/1‑101 (MQ=255)
           gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAcccc                                                                                         >  2:4273049/1‑100 (MQ=255)
           gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAcccc                                                                                         >  1:490578/1‑100 (MQ=255)
           gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAcccc                                                                                         <  1:1261833/101‑2 (MQ=37)
            tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAccccg                                                                                        >  1:3080434/1‑99 (MQ=255)
              ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTTCG.C.CAATTcacccccgg                                                                                       >  2:869806/1‑93 (MQ=255)
              ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCggg                                                                                       <  1:4226843/101‑1 (MQ=37)
                tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGag                                                                                     >  1:156649/1‑100 (MQ=37)
                 tctttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatg                                                                                    <  2:2056095/98‑3 (MQ=25)
                   aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgtt                                                                                  >  1:414096/1‑97 (MQ=25)
                   aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgtt                                                                                  <  2:3580254/101‑5 (MQ=255)
                   aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgtt                                                                                  <  1:2475399/101‑5 (MQ=255)
                    ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgttt                                                                                 >  2:776293/1‑96 (MQ=255)
                     tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                >  1:2432459/1‑101 (MQ=255)
                     tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                <  2:3533268/101‑1 (MQ=255)
                      gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                               <  1:4360063/101‑1 (MQ=255)
                      gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                               <  2:2238252/101‑1 (MQ=255)
                      gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                               >  2:4292133/1‑101 (MQ=25)
                      gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCA.TTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                               <  1:4280607/101‑1 (MQ=255)
                        aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                             >  1:2653741/1‑101 (MQ=255)
                         cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGcc                                                                            <  1:2834175/101‑1 (MQ=255)
                          gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                           >  2:1175606/1‑101 (MQ=255)
                          gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                           >  2:255564/1‑101 (MQ=255)
                          gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                           >  2:4168684/1‑101 (MQ=255)
                            cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGCTGTTTGGGGCaag                                                                         >  2:1649210/1‑100 (MQ=19)
                            cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                         >  1:994935/1‑100 (MQ=21)
                            cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                         <  1:4190558/101‑2 (MQ=255)
                             tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCTCCTGGCC.CTTCGCC.CAACTCACCCCGGGATGTTTGGGTCaggg                                                                        >  1:216287/1‑98 (MQ=255)
                               tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGcccaggtg                                                                      >  1:1016181/1‑95 (MQ=19)
                               tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                      <  1:808522/101‑5 (MQ=255)
                                ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                     <  1:2240324/101‑6 (MQ=16)
                                 tAATTTCGAATCTTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                    <  2:1708772/101‑7 (MQ=255)
                                 tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                    <  2:2120018/101‑7 (MQ=255)
                                  aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                   <  2:1255025/101‑8 (MQ=255)
                                   aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                <  2:2837212/101‑1 (MQ=16)
                                    tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCCCCTGGCC.TTTCG.GCCAATTCATCCCCGGGTGTTTGGGCCAAGgggaaaaa                                                                 >  1:117431/1‑96 (MQ=255)
                                      tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                             >  1:2768825/1‑98 (MQ=255)
                                       cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                            >  2:3693851/1‑97 (MQ=255)
                                        gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                           <  1:260799/101‑6 (MQ=255)
                                         aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                          >  2:658502/1‑95 (MQ=255)
                                         aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                          <  1:3599323/101‑7 (MQ=12)
                                          aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                         >  1:4212137/1‑94 (MQ=255)
                                          aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                         >  1:1660196/1‑94 (MQ=255)
                                           tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                        >  2:1856447/1‑93 (MQ=12)
                                            ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                       <  2:2202451/101‑10 (MQ=9)
                                            ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                       <  1:195903/101‑10 (MQ=9)
                                             cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                      <  1:571807/101‑11 (MQ=255)
                                                 cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                <  1:2635474/101‑1 (MQ=255)
                                                    gAATTTTGCAAGGAGGGGAGAGGTGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                             <  2:588840/101‑1 (MQ=255)
                                                     aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                            <  1:4426896/101‑1 (MQ=9)
                                                       ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                          >  2:3959138/1‑101 (MQ=9)
                                                        tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                         <  1:3147993/101‑1 (MQ=9)
                                                           gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                      >  1:4349251/1‑101 (MQ=255)
                                                           gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                      <  2:3218898/101‑1 (MQ=9)
                                                             aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATAc                                    <  1:776373/101‑1 (MQ=255)
                                                             aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                    >  2:235636/1‑101 (MQ=9)
                                                              aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                   >  2:438421/1‑101 (MQ=255)
                                                                gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                 >  2:344212/1‑101 (MQ=9)
                                                                 aggGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                <  1:3959394/101‑1 (MQ=9)
                                                                  ggggAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                               <  2:156628/101‑1 (MQ=255)
                                                                         gaggggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                          <  1:4168945/92‑1 (MQ=9)
                                                                         gaggggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                          <  2:3136530/92‑1 (MQ=9)
                                                                          gggggccctcccccACCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                         <  2:3521807/91‑1 (MQ=12)
                                                                          aggggcgcttccccTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGccc                         <  2:1759983/91‑1 (MQ=255)
                                                                          aggggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                         >  2:46779/9‑101 (MQ=16)
                                                                          aggggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                         <  2:1952941/93‑1 (MQ=255)
                                                                           ggggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                        <  1:1451990/94‑1 (MQ=255)
                                                                             ggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                      <  2:3561214/96‑1 (MQ=255)
                                                                             ggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                      <  1:2976580/96‑1 (MQ=255)
                                                                              gcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                     <  1:2348519/97‑1 (MQ=255)
                                                                               cgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                    >  1:1837180/4‑101 (MQ=16)
                                                                               cgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                    >  1:311941/4‑101 (MQ=16)
                                                                                gctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                   >  1:2198103/3‑101 (MQ=16)
                                                                                 ctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                  >  2:1163031/2‑101 (MQ=16)
                                                                                  tGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                 >  2:132586/1‑101 (MQ=255)
                                                                                  tGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                 >  2:3732371/1‑101 (MQ=19)
                                                                                   gCCCCTCCCGGCC.TCTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                <  1:3849427/101‑1 (MQ=255)
                                                                                    ccccTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg               <  2:909483/101‑1 (MQ=255)
                                                                                    ccccTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg               <  1:4247761/101‑1 (MQ=255)
                                                                                     ccctccctggc.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc              <  1:410371/91‑1 (MQ=255)
                                                                                     cccTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc              >  1:2066798/1‑101 (MQ=255)
                                                                                        tcctGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc           >  2:3470728/4‑101 (MQ=255)
                                                                                              cc.TTTCG.GCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa     <  1:1762462/101‑1 (MQ=25)
                                                                                              cc.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa     <  2:1016096/101‑1 (MQ=19)
                                                                                               c.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg    >  2:3342118/1‑101 (MQ=255)
                                                                                                 tttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg   >  1:612002/1‑101 (MQ=19)
                                                                                                  ttCG.GCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc  <  1:1594419/101‑1 (MQ=25)
                                                                                                  ttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc  <  2:3656425/101‑1 (MQ=19)
                                                                                                        |                                                                                                   
CTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCC.TTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGC  >  NC_014034/1696076‑1696276

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG