Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,841 +C 96.3% intergenic (‑94/+99) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8411.→C96.3% 13.9 ‑4.2 ‑0.0188intergenic (‑94/+99)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (67/114):  ref base (6/1):  total (73/115)
Fisher's exact test strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.34e-01

Only 100 of 215 total aligned reads displayed.

GTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.CCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA  >  NC_014034/1734741‑1734932
                                                                                                       |                                                                                              
 tgtCATCGAACCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.c                                                                                               <  2:4170474/101‑1 (MQ=255)
   tCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.ccc                                                                                             >  2:4004032/1‑101 (MQ=255)
    cATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.cccc                                                                                            >  1:3303071/1‑101 (MQ=255)
        gAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.ccccccgt                                                                                        <  1:3592066/101‑4 (MQ=255)
         aacccTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.ccccccgtc                                                                                       <  1:3229280/98‑5 (MQ=255)
          agccTCCGGGGAATGGCGCCTCCTAGCAGCCGGGGCGGGCACGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.ccccccgtcc                                                                                      <  2:1565131/99‑6 (MQ=38)
          aaCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.ccccccgtcc                                                                                      >  1:600902/1‑96 (MQ=255)
            ccTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTg                                                                                     <  1:3198735/101‑1 (MQ=255)
             cTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGc                                                                                    >  1:4005827/1‑101 (MQ=255)
               cgggggAATGGCGCATCGAAACGGCCGGGTCGGGCAAGCCAACGCCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCgg                                                                                  <  2:736823/99‑1 (MQ=25)
               ccGGGGAATGGCGCATCCTAGCAGCGGGGGGGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCgg                                                                                  <  1:2132685/101‑1 (MQ=38)
                cGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGa                                                                                 >  2:3617784/1‑101 (MQ=255)
                cGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGa                                                                                 >  2:1951098/1‑101 (MQ=255)
                 ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGAc                                                                                >  1:2484427/1‑101 (MQ=255)
                  gggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACt                                                                               <  1:2288389/101‑1 (MQ=255)
                   ggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTc                                                                              >  2:4154457/1‑101 (MQ=255)
                   ggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTc                                                                              >  2:3426869/1‑101 (MQ=255)
                     aaTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTccc                                                                            >  1:869240/1‑101 (MQ=255)
                       tGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTccccc                                                                          >  1:1885721/1‑101 (MQ=255)
                        ggggcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTcccccc                                                                         <  2:1161360/98‑1 (MQ=255)
                         gcgcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.g                                                                       <  1:3444358/101‑1 (MQ=255)
                         gcgcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.g                                                                       <  2:692567/101‑1 (MQ=255)
                           gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.ggg                                                                     >  1:672385/1‑101 (MQ=255)
                           gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.ggg                                                                     <  1:1875790/101‑1 (MQ=255)
                           ctacccgagcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.ggg                                                                     <  1:3657743/94‑1 (MQ=25)
                           ccctCCTCGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.ggg                                                                     <  2:2131813/98‑1 (MQ=25)
                            cATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGa                                                                    >  2:2340325/1‑101 (MQ=255)
                             agccgggcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGat                                                                   <  1:792191/95‑1 (MQ=25)
                             aTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGat                                                                   >  2:3699147/1‑101 (MQ=255)
                              tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGata                                                                  <  2:626711/101‑1 (MQ=255)
                              tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGAGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.cgggca                                                                  >  1:3823560/1‑98 (MQ=37)
                               ccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGatat                                                                 <  2:177687/101‑1 (MQ=255)
                                 tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATAttt                                                               >  2:2176303/1‑101 (MQ=255)
                                  agcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.TCC.CCCCCGTCCTGCGGCCTcccccccagccagccc                                                               >  1:904482/1‑91 (MQ=25)
                                   gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTAt                                                             <  1:2642214/101‑1 (MQ=255)
                                    cagcGGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATg                                                            <  2:2301188/101‑1 (MQ=255)
                                    cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATg                                                            >  1:3389174/1‑101 (MQ=255)
                                    cagcCGGGGCGGGAAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATg                                                            <  2:4093794/101‑1 (MQ=255)
                                     agcCGGGGGGGGCAAGCCAAAGGCTGGCGGTGGGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATgg                                                           <  2:2791345/101‑1 (MQ=18)
                                     agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATgg                                                           >  1:2240235/1‑101 (MQ=255)
                                      gcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGc                                                          >  2:3349358/1‑101 (MQ=255)
                                       ccGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCa                                                         <  1:2525368/101‑1 (MQ=255)
                                       ccGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCa                                                         <  2:3639865/101‑1 (MQ=255)
                                        cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCaa                                                        <  1:4271323/101‑1 (MQ=255)
                                        cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCaa                                                        >  2:2971250/1‑101 (MQ=255)
                                        cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCaa                                                        <  1:2705353/101‑1 (MQ=255)
                                        cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGACCTTTTTGGcca                                                        >  1:1634872/1‑99 (MQ=21)
                                         ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAg                                                       <  2:2549089/101‑1 (MQ=255)
                                         ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAg                                                       >  1:2059241/1‑101 (MQ=255)
                                         ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAg                                                       >  1:707451/1‑101 (MQ=255)
                                         ggggCAGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAg                                                       >  2:2195979/1‑101 (MQ=255)
                                          gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGa                                                      >  2:2502495/1‑101 (MQ=255)
                                             cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGa                                                   <  2:4032022/101‑1 (MQ=255)
                                               ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGaaa                                                 >  2:1060486/1‑101 (MQ=255)
                                                gcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAAc                                                >  2:3270054/1‑101 (MQ=255)
                                                gcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAAc                                                <  1:962864/101‑1 (MQ=255)
                                                 cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACt                                               <  2:1579545/101‑1 (MQ=255)
                                                   aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGc                                             >  2:1445076/1‑101 (MQ=255)
                                                   aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGc                                             <  1:2657759/101‑1 (MQ=255)
                                                      cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGc                                         >  2:3872315/1‑101 (MQ=255)
                                                      cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGACATTTATGGCAAGATGAAACTGC.GGc                                         >  2:1983972/1‑101 (MQ=255)
                                                       aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGcc                                        >  2:3400753/1‑101 (MQ=255)
                                                       aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGcc                                        <  2:1075417/101‑1 (MQ=255)
                                                          ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                     <  1:1737590/101‑1 (MQ=255)
                                                           gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                    <  1:1852554/101‑1 (MQ=255)
                                                            cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                   >  2:2884406/1‑101 (MQ=255)
                                                             tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGGCTTCATc                                  >  1:3027659/1‑101 (MQ=255)
                                                              gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                 >  2:1721175/1‑101 (MQ=255)
                                                              gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                 <  2:152508/101‑1 (MQ=255)
                                                               aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTAGGGCAAGATGAAACTGG.GGCCTTCATCtt                                >  1:4032815/1‑101 (MQ=18)
                                                                cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                               <  2:2710461/101‑1 (MQ=255)
                                                                 gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                              <  2:1001358/101‑1 (MQ=255)
                                                                  cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGcc                             >  2:3731071/1‑101 (MQ=255)
                                                                    gCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                           <  2:1363499/101‑1 (MQ=255)
                                                                     cGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                          <  1:506585/101‑1 (MQ=255)
                                                                       tCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                        <  2:308137/101‑1 (MQ=255)
                                                                       tCAGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                        <  1:298829/101‑1 (MQ=255)
                                                                         aGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                      <  1:3747060/101‑1 (MQ=255)
                                                                         aGCCGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                      <  1:1477346/101‑1 (MQ=255)
                                                                           ccGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                    <  2:3375367/101‑1 (MQ=255)
                                                                           ccGACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                    >  1:1114658/1‑101 (MQ=255)
                                                                             gACGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                  <  2:818815/101‑1 (MQ=255)
                                                                              aCGGGCCGGGCAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                <  1:3569792/101‑1 (MQ=255)
                                                                               cgggccgggcAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg               >  2:3951608/1‑101 (MQ=255)
                                                                                  tgacggacggggggCTC.T.G.CCCCCCGGCTTCG..CTCCCCCC.GGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg           >  1:3088440/9‑101 (MQ=2)
                                                                                   ccgggcAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg           >  1:772477/1‑101 (MQ=255)
                                                                                    cgggcAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc          <  2:3818961/101‑1 (MQ=255)
                                                                                    cgggcAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc          >  2:3917176/1‑101 (MQ=255)
                                                                                    cgggcAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc          <  1:2751702/101‑1 (MQ=255)
                                                                                     cggacggggggCTC.T.G.CCCCCCGGCTTCG..CTCCCCCC.GGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat        >  1:3508645/6‑98 (MQ=2)
                                                                                     cggacggggggCTC.T.G.CCCCCCGGCTTCG..CTCCCCCC.GGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat        >  2:1680161/6‑98 (MQ=2)
                                                                                       gcAGGGGGGCGCTT.C.CCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg       <  1:4154718/101‑1 (MQ=255)
                                                                                        cAGGGGGGCGC.T.GCCCCCCGTCCTGCGTACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg      <  2:708675/101‑1 (MQ=255)
                                                                                        cAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCCGGGGATTTTTTTGCCAAGAGGAAATGGG.GCCCTTATTTTTCCCAAAAAATTCCC.GGGGGGGggg       >  1:1651469/1‑101 (MQ=2)
                                                                                        cAGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg      <  1:3699400/101‑1 (MQ=255)
                                                                                         agcggggCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATCTTTATGGCAAGATGAAACCGC.GGCCTTCATCTTGCCATAAATATCCC.GGGTTGTGGGGc     >  2:4108659/4‑101 (MQ=14)
                                                                                         aGGGGGGCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc     <  2:3302839/101‑1 (MQ=255)
                                                                                          ggggggCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa    <  1:1595696/101‑1 (MQ=255)
                                                                                            ggggCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga  >  1:1256737/1‑101 (MQ=255)
                                                                                            ggggCGC.T.GCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga  <  2:600834/101‑1 (MQ=255)
                                                                                                       |                                                                                              
GTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGC.T.G.CCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA  >  NC_014034/1734741‑1734932

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG