Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,767 +G 1.8% intergenic (+4/+131) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7671.→G 1.8% 2.2 ‑2.3 ‑0.0167intergenic (+4/+131)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/3):  ref base (90/74):  total (90/77)
Fisher's exact test strand distribution p-value = 9.60e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.64e-01

Only 100 of 190 total aligned reads displayed.

GCGCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.GGGGCGCT.GCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCG  >  NC_014034/1740667‑1740863
                                                                                                      |                                                                                                   
gcgcgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGc                                                                                                      >  1:3713583/1‑101 (MQ=255)
gcgcgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGc                                                                                                      >  1:2147821/1‑101 (MQ=255)
  gcgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.Ga                                                                                                   <  1:3057491/101‑1 (MQ=255)
   cgAGACGACCGTGGCCGCCTGCGGTTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.g                                                                                                 <  1:3229986/101‑1 (MQ=255)
    gagaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.gg                                                                                                >  1:2828798/1‑101 (MQ=255)
     agaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.ggg                                                                                               >  2:860567/1‑101 (MQ=255)
          aTCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGcacctgcaggg                                                                                            <  2:1394723/101‑11 (MQ=255)
           tCGTGACCGCCGGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.gggggcgct                                                                                         <  2:2515602/101‑6 (MQ=255)
            cGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.g                                                                                        >  2:3950488/1‑101 (MQ=255)
            cGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.g                                                                                        >  2:1842027/1‑101 (MQ=255)
             gTGCCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gc                                                                                       <  1:2962826/101‑1 (MQ=255)
             gTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gc                                                                                       >  1:2932177/1‑101 (MQ=255)
                gccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccc                                                                                    <  2:3701473/100‑1 (MQ=255)
                 ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gccccc                                                                                   <  1:2466878/101‑1 (MQ=255)
                 ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gccccc                                                                                   <  2:3201063/101‑1 (MQ=255)
                  cGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATATGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccg                                                                                  <  2:217295/101‑2 (MQ=255)
                   gCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgc                                                                                 <  2:310445/101‑3 (MQ=255)
                     cggggggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccg                                                                               <  1:2615235/97‑5 (MQ=255)
                     cTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccg                                                                               >  1:1709852/1‑97 (MQ=255)
                     cTGCGGGTGCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATGCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccg                                                                               <  2:1021111/101‑5 (MQ=255)
                      tGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccga                                                                              >  1:1598597/1‑96 (MQ=255)
                         gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGGGCT.GCCCCCGC.CGAGGc                                                                          >  2:1189904/1‑101 (MQ=255)
                          ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCg                                                                         >  1:3993281/1‑101 (MQ=255)
                           gTTCGAGCCAACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGa                                                                        >  2:266268/1‑101 (MQ=255)
                            ttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGAt                                                                       >  2:2253122/1‑101 (MQ=255)
                                aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCt                                                                   >  2:1226908/1‑101 (MQ=255)
                                 gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTc                                                                  <  1:1935872/101‑1 (MQ=255)
                                 cccGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTc                                                                  <  2:2022964/100‑1 (MQ=255)
                                  ccGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCg                                                                 <  2:699342/101‑1 (MQ=255)
                                    gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGc                                                               >  1:1042844/1‑101 (MQ=255)
                                      cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGccc                                                             >  2:4227474/1‑101 (MQ=255)
                                       gaaagacCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGgccc                                                            <  2:3772809/99‑1 (MQ=255)
                                        gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGccccc                                                           <  2:1592236/101‑1 (MQ=255)
                                         aagaaCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                          <  2:2941493/101‑1 (MQ=255)
                                         aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                          >  2:3556905/1‑101 (MQ=255)
                                         aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                          <  1:4186169/101‑1 (MQ=255)
                                          agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                         >  2:1200108/1‑101 (MQ=255)
                                          agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                         <  2:391808/101‑1 (MQ=255)
                                           caaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                        >  1:4409732/2‑101 (MQ=255)
                                            aaCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTCCCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                       <  1:1838521/101‑1 (MQ=255)
                                            aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                       >  2:828201/1‑101 (MQ=255)
                                             aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                      >  2:2150703/1‑101 (MQ=255)
                                             aCCGCTTCTGGACCATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                      <  1:3833070/101‑1 (MQ=255)
                                               cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                    <  1:3922143/101‑1 (MQ=38)
                                               cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                    >  1:1668066/1‑101 (MQ=255)
                                                gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                   >  2:1965476/1‑101 (MQ=255)
                                                 tttCTGGTCTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                  <  2:3397021/100‑1 (MQ=38)
                                                 cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                  >  2:2131235/1‑101 (MQ=38)
                                                 cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                  >  1:869585/1‑101 (MQ=38)
                                                 cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                  >  2:2009158/1‑101 (MQ=38)
                                                  ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGCTGCCTCTGCCGCCCCGGaacttt                                                 >  2:598852/1‑96 (MQ=255)
                                                  ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                 >  1:2314266/1‑101 (MQ=38)
                                                   tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                >  1:1095140/1‑101 (MQ=38)
                                                    cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                               >  2:386066/1‑101 (MQ=38)
                                                    cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                               <  1:4237023/101‑1 (MQ=38)
                                                         cTATCTGGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                          <  2:1157438/101‑1 (MQ=37)
                                                          tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                         <  1:3450904/101‑1 (MQ=38)
                                                          tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                         <  2:1471731/101‑1 (MQ=38)
                                                           aTCTCGAAAGCAAATCCGCCGCGATCTCCCGGCTTT.GAGGGCGGG.GGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                        <  2:1671512/101‑1 (MQ=25)
                                                           aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                        >  2:557793/1‑101 (MQ=38)
                                                            tctcGAAAGCAAATCGGCGGCGTTCTCCCGGCTTT.GAGGGC.GAGGGGGGGCG.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                       <  1:1189995/101‑1 (MQ=21)
                                                            tctcGAAAGCAAATCCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                       <  1:4377154/101‑1 (MQ=37)
                                                            tctcGAAAGCAAATACGCCGCGATCTACCGGCTTT.GGGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                       <  1:1982144/101‑1 (MQ=37)
                                                            tctcGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                       <  2:4030771/101‑1 (MQ=37)
                                                             ctcGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                      >  2:4188133/1‑101 (MQ=37)
                                                              tcGAAAGCAAATACGCCGCGATCTCCCGGCTTT.GGGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                     <  1:875283/101‑1 (MQ=37)
                                                               cGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                    >  1:772345/1‑101 (MQ=37)
                                                               cGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                    >  1:719791/1‑101 (MQ=37)
                                                                gAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                   >  2:3997925/1‑101 (MQ=37)
                                                                 aaaGCAAATGCGCCGCGATCTCCCGGTTTG.GAGGGC.GAGGGGGCGCT.GCCGCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                  <  1:2438197/101‑1 (MQ=21)
                                                                 aaaGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                  >  2:231992/1‑101 (MQ=37)
                                                                  aaGAAAATACGCCGCGATCTACCGGCTTT.GGGGGC.GACGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAgg                                 <  1:3104572/101‑1 (MQ=37)
                                                                   aGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                <  1:266295/101‑1 (MQ=38)
                                                                    gCAAATGCGCCGCCATCTACCGGCTTT.GGGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                               <  1:1863089/101‑1 (MQ=25)
                                                                     cAAATCCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                              <  2:1928928/101‑1 (MQ=37)
                                                                     cAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                              >  2:3265180/1‑101 (MQ=37)
                                                                     aaaaTCCCCCGCGATCTCCGGGTTTG.GAGGGCGGG.GGGGCGCC.CCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                              <  1:498498/100‑1 (MQ=16)
                                                                      aagtACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                             >  1:2555887/4‑101 (MQ=37)
                                                                      aaaTACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGACATTTCGAGCAAGATGAAAGGCATc                             >  1:1260790/1‑101 (MQ=38)
                                                                        atccgccgcGATCTCCCGGCTTT.GGGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                           <  1:3224992/98‑1 (MQ=25)
                                                                        aTACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                           <  2:1502949/101‑1 (MQ=38)
                                                                        aTACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                           >  2:3935767/1‑101 (MQ=37)
                                                                         tACGCCGCGATCTCCCGGCTTG.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                          <  2:957760/101‑1 (MQ=37)
                                                                         tACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                          >  2:2707261/1‑101 (MQ=37)
                                                                             ccccgccccaCCGGCTTG.GGGGGC.GA.GGGGGCCC.GCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                      <  1:3780176/92‑1 (MQ=21)
                                                                              cgcgATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGGCTCGGTCCCCCGTGATATTTCGAGCCAGATGAAAGGCATCCGGtttaa                     >  2:1080958/1‑99 (MQ=25)
                                                                               gcgATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                    >  1:3489667/1‑101 (MQ=37)
                                                                                cgATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                   >  1:1723883/1‑101 (MQ=37)
                                                                                 gATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                  <  2:1846174/101‑1 (MQ=37)
                                                                                  aTCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                 >  1:3067087/1‑101 (MQ=25)
                                                                                   tCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCTGTTTCATCTTg                >  1:1379178/1‑101 (MQ=25)
                                                                                   tCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                >  1:363956/1‑101 (MQ=25)
                                                                                    cTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAATATGAAAGGCATCCGGTTTCATCTTGc               >  1:3369866/1‑101 (MQ=25)
                                                                                     tgccGGCTTT.GGGGGC.GA.GGGG.GCTCCCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt              <  1:1965605/99‑1 (MQ=18)
                                                                                      aCCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGAATCCGGTTTCATCTTGCtt             >  1:2227801/1‑101 (MQ=25)
                                                                                       ccGGTTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc            <  1:653359/101‑1 (MQ=25)
                                                                                       ccGGCTTTGGGGGGG.GG.GGGGCGCG.GCCCCCGC.GGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc            <  1:3869579/101‑1 (MQ=18)
                                                                                         ggCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaa          >  2:3066202/1‑101 (MQ=25)
                                                                                           cTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt        >  1:1022396/1‑101 (MQ=25)
                                                                                              t.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc     <  1:2602701/101‑1 (MQ=25)
                                                                                                      |                                                                                                   
GCGCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.GGGGCGCT.GCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCG  >  NC_014034/1740667‑1740863

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG