Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,769 +G 97.5% intergenic (+6/+129) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7691.→G97.5% 4.9 ‑3.0 ‑0.2160intergenic (+6/+129)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (83/72):  ref base (0/4):  total (83/77)
Fisher's exact test strand distribution p-value = 5.00e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.41e-01

Only 100 of 186 total aligned reads displayed.

GCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.GGGGCGCT.GCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCG  >  NC_014034/1740669‑1740863
                                                                                                       |                                                                                                
gcgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.Ga                                                                                                   <  1:3057491/101‑1 (MQ=255)
 ggagaGGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.g                                                                                                 <  2:3496469/100‑1 (MQ=255)
 cgAGACGACCGTGGCCGCCTGCGGTTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.g                                                                                                 <  1:3229986/101‑1 (MQ=255)
  gagaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.gg                                                                                                >  1:2828798/1‑101 (MQ=255)
   agaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.ggg                                                                                               >  2:860567/1‑101 (MQ=255)
   agaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.ggg                                                                                               >  2:1935743/1‑101 (MQ=255)
     aCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.ggggg                                                                                             >  2:3073347/1‑100 (MQ=255)
      cGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.gggggc                                                                                            <  2:2725201/101‑3 (MQ=255)
          cGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.g                                                                                        >  2:3950488/1‑101 (MQ=255)
          cGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.g                                                                                        >  2:1842027/1‑101 (MQ=255)
           gTGCCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gc                                                                                       <  1:2962826/101‑1 (MQ=255)
           gTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gc                                                                                       >  1:2932177/1‑101 (MQ=255)
               ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gccccc                                                                                   <  1:2466878/101‑1 (MQ=255)
               ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gccccc                                                                                   <  2:3201063/101‑1 (MQ=255)
                cGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATATGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccg                                                                                  <  2:217295/101‑2 (MQ=255)
                 gCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgc                                                                                 <  2:310445/101‑3 (MQ=255)
                  ccTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgcc                                                                                <  2:2147682/101‑4 (MQ=255)
                   cggggggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccg                                                                               <  1:2615235/97‑5 (MQ=255)
                   cTGCGGTTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccg                                                                               <  2:140894/101‑5 (MQ=255)
                   cTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccg                                                                               >  2:3382216/1‑97 (MQ=255)
                   cTGCGGGTGCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATGCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccg                                                                               <  2:1021111/101‑5 (MQ=255)
                    tGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.Gcccccgccga                                                                              >  1:1598597/1‑96 (MQ=255)
                       gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGGGCT.GCCCCCGC.CGAGGc                                                                          >  2:1189904/1‑101 (MQ=255)
                        ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCACCCGC.CGAGGCg                                                                         >  2:255495/1‑101 (MQ=255)
                         gTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGa                                                                        >  2:2684494/1‑101 (MQ=255)
                          ttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGAt                                                                       >  2:2253122/1‑101 (MQ=255)
                              aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCt                                                                   >  2:1226908/1‑101 (MQ=255)
                              aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCt                                                                   <  2:3001334/101‑1 (MQ=255)
                              aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCt                                                                   >  1:3096914/1‑101 (MQ=255)
                               gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTc                                                                  <  2:1472956/101‑1 (MQ=255)
                               gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTc                                                                  <  1:1935872/101‑1 (MQ=255)
                                ccGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCg                                                                 <  2:699342/101‑1 (MQ=255)
                                  gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGc                                                               >  1:1042844/1‑101 (MQ=255)
                                    cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGccc                                                             >  2:4227474/1‑101 (MQ=255)
                                     ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAAGACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGcccc                                                            <  1:1729493/101‑1 (MQ=255)
                                      gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGccccc                                                           <  2:1592236/101‑1 (MQ=255)
                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                         >  2:1200108/1‑101 (MQ=255)
                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                         <  2:391808/101‑1 (MQ=255)
                                         caaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                        >  1:4409732/2‑101 (MQ=255)
                                          aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                       >  2:828201/1‑101 (MQ=255)
                                           aCCGCTTCTGGACCATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                      <  1:3833070/101‑1 (MQ=255)
                                             cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                    <  1:3922143/101‑1 (MQ=38)
                                             cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                    >  1:1668066/1‑101 (MQ=255)
                                               cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                  <  1:1719301/101‑1 (MQ=38)
                                               cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                  >  1:869585/1‑101 (MQ=38)
                                                ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGCTGCCTCTGCCGCCCCGGaacttt                                                 >  2:598852/1‑96 (MQ=255)
                                                ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                 >  1:2314266/1‑101 (MQ=38)
                                                 tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                >  1:1095140/1‑101 (MQ=38)
                                                  cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                               <  1:4237023/101‑1 (MQ=38)
                                                  cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                               >  2:386066/1‑101 (MQ=38)
                                                  cTGGACTATCTCGAAAGAAAATCCGCCGCAATCTACCGGCTTT.GAGGGC.GAGGGGGCGCG.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                               <  2:4108684/101‑1 (MQ=37)
                                                   tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                              >  1:4200729/1‑101 (MQ=38)
                                                       cTATCTGGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                          <  2:1157438/101‑1 (MQ=37)
                                                        tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                         <  1:3450904/101‑1 (MQ=38)
                                                         aTCTCGAAAGCAAATCCGCCGCGATCTCCCGGCTTT.GAGGGCGGG.GGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                        <  2:1671512/101‑1 (MQ=25)
                                                         aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                        >  2:557793/1‑101 (MQ=38)
                                                         aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                        >  2:1979814/1‑101 (MQ=38)
                                                          tctcGAAAGCAAATCGGCGGCGTTCTCCCGGCTTT.GAGGGC.GAGGGGGGGCG.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                       <  1:1189995/101‑1 (MQ=21)
                                                          tctcGAAAGCAAATCCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                       <  1:4377154/101‑1 (MQ=37)
                                                          tctcGAAAGCAAATACGCCGCGATCTACCGGCTTT.GGGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                       <  1:1982144/101‑1 (MQ=37)
                                                            tcGAAAGCAAATACGCCGCGATCTCCCGGCTTT.GGGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                     <  1:875283/101‑1 (MQ=37)
                                                             cGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                    >  1:719791/1‑101 (MQ=37)
                                                             cGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                    >  1:772345/1‑101 (MQ=37)
                                                              gAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                   >  1:2097882/1‑101 (MQ=37)
                                                              gAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                   >  2:3997925/1‑101 (MQ=37)
                                                               aaaGCAAATGCGCCGCGATCTCCCGGTTTG.GAGGGC.GAGGGGGCGCT.GCCGCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                  <  1:2438197/101‑1 (MQ=21)
                                                                 aGCAAATCCGCCGCGATCTACCGGCTTG.GAGGGC.GAGGGGGCGCC.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                <  2:3660395/101‑1 (MQ=37)
                                                                 aGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                <  1:266295/101‑1 (MQ=38)
                                                                 aGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                <  2:2399871/101‑1 (MQ=38)
                                                                  gCAAATGCGCCGCCATCTACCGGCTTT.GGGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                               <  1:1863089/101‑1 (MQ=25)
                                                                   cAAATCCGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                              <  2:1928928/101‑1 (MQ=37)
                                                                   aaaaTCCCCCGCGATCTCCGGGTTTG.GAGGGCGGG.GGGGCGCC.CCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                              <  1:498498/100‑1 (MQ=16)
                                                                    aagtACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                             >  1:2555887/4‑101 (MQ=37)
                                                                    aaaTACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGACATTTCGAGCAAGATGAAAGGCATc                             >  1:1260790/1‑101 (MQ=38)
                                                                    aaaCACGCCGCGATCGAGCGGCTTG.GAGGGC.GAGGGGGCCGT.GCCCCCGT.CGAGGCGATGCCTCGGGCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                             <  1:3687793/101‑1 (MQ=21)
                                                                      atccgccgcGATCTACCGGCTTG.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                           <  2:228001/98‑1 (MQ=37)
                                                                      aTACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTTGAGCAAGAAGaaaaagaaccg                           >  1:2609799/1‑93 (MQ=25)
                                                                      aTACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCCAGATGAAAGGCATCCg                           >  1:3177060/1‑101 (MQ=37)
                                                                      aTACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                           >  2:3935767/1‑101 (MQ=37)
                                                                      aTACGCCGCGATCTACCGGCTTG.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                           <  2:2069295/101‑1 (MQ=37)
                                                                       tACGCCGCGATCTCCCGGCTTG.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                          <  2:957760/101‑1 (MQ=37)
                                                                       tACGCCGCGATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                          >  1:168708/1‑101 (MQ=37)
                                                                           ccccgccccaCCGGCTTG.GGGGGC.GA.GGGGGCCC.GCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                      <  1:3780176/92‑1 (MQ=21)
                                                                            cgcgATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGGCTCGGTCCCCCGTGATATTTCGAGCCAGATGAAAGGCATCCGGtttaa                     >  2:1080958/1‑99 (MQ=25)
                                                                             gcgATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                    >  1:3489667/1‑101 (MQ=37)
                                                                             gcgATCGACCGGCTTT.GGGGGC.GAGGGGGCGCT.CCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                    <  2:3713330/101‑1 (MQ=25)
                                                                               gATCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                  <  2:1846174/101‑1 (MQ=37)
                                                                                aTCTACCGGCTTT.GAGGGCGGG.GGGGCGCG.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                 <  2:419930/101‑1 (MQ=25)
                                                                                aTCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                 >  1:3067087/1‑101 (MQ=25)
                                                                                 tCTACCGGCTTT.GAGGGCGGG.GGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                <  2:2010174/101‑1 (MQ=25)
                                                                                 tCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCTGTTTCATCTTg                >  1:1379178/1‑101 (MQ=25)
                                                                                 tCTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                >  1:363956/1‑101 (MQ=25)
                                                                                  cTACCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAATATGAAAGGCATCCGGTTTCATCTTGc               >  1:3369866/1‑101 (MQ=25)
                                                                                   tgccGGCTTT.GGGGGC.GA.GGGG.GCTCCCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt              <  1:1965605/99‑1 (MQ=18)
                                                                                    aCCGGCTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGAATCCGGTTTCATCTTGCtt             >  1:2227801/1‑101 (MQ=25)
                                                                                     ccGGTTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc            <  1:653359/101‑1 (MQ=25)
                                                                                     ccGGCTTTGGGGGGG.GG.GGGGCGCG.GCCCCCGC.GGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc            <  1:3869579/101‑1 (MQ=18)
                                                                                         cTTT.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt        >  1:1022396/1‑101 (MQ=25)
                                                                                           tt.GAGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCGCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc      >  2:3851773/1‑101 (MQ=25)
                                                                                               aGGGC.GAGGGGGCGCT.GCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc   >  1:4079193/1‑101 (MQ=25)
                                                                                                       |                                                                                                
GCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGGC.GA.GGGGCGCT.GCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCG  >  NC_014034/1740669‑1740863

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG