Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,785 +C 4.5% intergenic (+22/+113) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7851.→C 4.5% 18.1 ‑0.3 ‑0.1176intergenic (+22/+113)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/5):  ref base (82/86):  total (85/91)
Fisher's exact test strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.05e-01

Only 100 of 181 total aligned reads displayed.

GGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGGCGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740692‑1740880
                                                                                                |                                                                                               
gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGGGCTGCCCCCGC.CGAGGc                                                                                           >  2:1189904/1‑101 (MQ=255)
gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGc                                                                                           <  2:396637/101‑1 (MQ=255)
gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGc                                                                                           <  2:4411032/101‑1 (MQ=255)
 ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCg                                                                                          >  1:3993281/1‑101 (MQ=255)
 ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCACCCGC.CGAGGCg                                                                                          >  2:255495/1‑101 (MQ=255)
  gTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGa                                                                                         >  2:2684494/1‑101 (MQ=255)
   ttggAGCCGACGGAAGAACCCCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGAt                                                                                        <  1:3814315/98‑1 (MQ=255)
   ttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGAt                                                                                        >  2:2253122/1‑101 (MQ=255)
       ggCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCt                                                                                    <  1:1465594/100‑1 (MQ=255)
          cGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCgg                                                                                 >  1:3235593/1‑101 (MQ=255)
           gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                >  1:1042844/1‑101 (MQ=255)
              gaaagacCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGgccc                                                                             <  2:3772809/99‑1 (MQ=255)
               gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGccccc                                                                            <  2:1592236/101‑1 (MQ=255)
                aagaaCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  2:2941493/101‑1 (MQ=255)
                aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  1:4186169/101‑1 (MQ=255)
                 agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  2:1200108/1‑101 (MQ=255)
                 agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  1:3891114/1‑101 (MQ=255)
                 agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          <  2:391808/101‑1 (MQ=255)
                  caaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                                         >  1:4409732/2‑101 (MQ=255)
                   aaCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        <  1:1838521/101‑1 (MQ=255)
                   aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        >  2:828201/1‑101 (MQ=255)
                   aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCcgg                                                                        >  1:3397258/1‑101 (MQ=255)
                    aCCGCTTCTGGACCATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                       <  1:3833070/101‑1 (MQ=255)
                      cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGata                                                                     >  1:1668066/1‑101 (MQ=255)
                        cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   <  1:1719301/101‑1 (MQ=38)
                        cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   >  2:2131235/1‑101 (MQ=38)
                         ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGCTGCCTCTGCCGCCCCGGaacttt                                                                  >  2:598852/1‑96 (MQ=255)
                         ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  >  2:2193770/1‑101 (MQ=38)
                          tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  1:2936507/1‑101 (MQ=38)
                          tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  1:1095140/1‑101 (MQ=38)
                           cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                <  1:4237023/101‑1 (MQ=38)
                           cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                >  2:386066/1‑101 (MQ=38)
                           cTGGACTATCTCGAAAGAAAATCCGCCGCAATCTACCGGCTTTGAGGGC.GAGGGGGCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                <  2:4108684/101‑1 (MQ=37)
                             ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              >  1:1971698/1‑101 (MQ=38)
                              gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             >  1:4388901/1‑101 (MQ=38)
                                cTATCTGGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           <  2:1157438/101‑1 (MQ=37)
                                 tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          <  2:1471731/101‑1 (MQ=38)
                                  aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         >  2:1979814/1‑101 (MQ=38)
                                   tctcGAAAGCAAATCGGCGGCGTTCTCCCGGCTTTGAGGGC.GAGGGGGGGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                                        <  1:1189995/101‑1 (MQ=21)
                                    ctcGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                                       <  1:1208813/101‑1 (MQ=37)
                                     tcGAAAGCAAATACGCCGCGATCTCCCGGCTTTGGGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      <  1:875283/101‑1 (MQ=37)
                                      cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                     >  1:772345/1‑101 (MQ=37)
                                      cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                     >  1:719791/1‑101 (MQ=37)
                                       gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  2:3997925/1‑101 (MQ=37)
                                       gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    <  1:960780/101‑1 (MQ=38)
                                       gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  1:2097882/1‑101 (MQ=37)
                                        aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                   >  2:231992/1‑101 (MQ=37)
                                         aaGAAAATACGCCGCGATCTACCGGCTTTGGGGGC.GACGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAgg                                                  <  1:3104572/101‑1 (MQ=37)
                                          aGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                                 <  1:266295/101‑1 (MQ=38)
                                           gCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                                <  1:2304358/101‑1 (MQ=37)
                                           gCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                                >  2:610190/1‑101 (MQ=37)
                                            cAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               <  2:1928928/101‑1 (MQ=37)
                                            cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               >  2:3265180/1‑101 (MQ=37)
                                            aaaaTCCCCCGCGATCTCCGGGTTTGGAGGGCGGG.GGGGCGCCCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               <  1:498498/100‑1 (MQ=16)
                                             aaaCACGCCGCGATCGAGCGGCTTGGAGGGC.GAGGGGGCCGTGCCCCCGT.CGAGGCGATGCCTCGGGCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              <  1:3687793/101‑1 (MQ=21)
                                               aTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTTGAGCAAGAAGaaaaagaaccg                                            >  1:2609799/1‑93 (MQ=25)
                                               aTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCCAGATGAAAGGCATCCg                                            >  1:3177060/1‑101 (MQ=37)
                                               aTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                                            <  1:2746347/101‑1 (MQ=38)
                                               aTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                                            >  2:3935767/1‑101 (MQ=37)
                                               aTACGCCGCGATCTACCGGCTTGGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                                            <  2:2069295/101‑1 (MQ=37)
                                                tACGCCGCGATCTCCCGGCTTGGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                                           <  2:957760/101‑1 (MQ=37)
                                                tACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                                           <  1:1946428/101‑1 (MQ=38)
                                                      gcgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                                     >  1:3489667/1‑101 (MQ=37)
                                                      gcgATCGACCGGCTTTGGGGGC.GAGGGGGCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                                     <  2:3713330/101‑1 (MQ=25)
                                                       cgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                                    >  1:1723883/1‑101 (MQ=37)
                                                        gATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                                   <  2:1846174/101‑1 (MQ=37)
                                                        gATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                                   >  1:2449416/1‑101 (MQ=37)
                                                         aTCTACCGGCTTTGAGGGCGGG.GGGGCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  2:419930/101‑1 (MQ=25)
                                                          tCTACCGGCTTTGAGGGCGGG.GGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                                 <  2:2010174/101‑1 (MQ=25)
                                                          tCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCTGTTTCATCTTg                                 >  1:1379178/1‑101 (MQ=25)
                                                             aCCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                              >  1:3059965/1‑101 (MQ=25)
                                                             aCCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGAATCCGGTTTCATCTTGCtt                              >  1:2227801/1‑101 (MQ=25)
                                                              ccGGTTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             <  1:653359/101‑1 (MQ=25)
                                                                ggCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaa                           >  2:3066202/1‑101 (MQ=25)
                                                                  cTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                         >  1:1022396/1‑101 (MQ=25)
                                                                    ttGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       >  1:1863980/1‑101 (MQ=25)
                                                                     tGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc                      >  2:1086619/1‑101 (MQ=25)
                                                                        ggggcgggggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                    <  2:2828591/93‑1 (MQ=21)
                                                                        gggC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCg                   >  1:432661/1‑101 (MQ=25)
                                                                             gagggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               >  1:86724/4‑101 (MQ=255)
                                                                              ggggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggggg              <  1:3073568/99‑1 (MQ=255)
                                                                              aggggtctcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggggg              <  2:1477291/93‑1 (MQ=21)
                                                                                ggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  1:3998181/101‑1 (MQ=255)
                                                                                ggggCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  2:869500/101‑1 (MQ=255)
                                                                                 gggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt           <  1:610258/101‑1 (MQ=255)
                                                                                 gggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt           <  1:2449576/101‑1 (MQ=255)
                                                                                 gggCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt           <  1:386105/101‑1 (MQ=255)
                                                                                  ggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGGAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTc          >  1:3765644/1‑101 (MQ=255)
                                                                                    cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTccc        <  2:3499016/101‑1 (MQ=255)
                                                                                    cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTccc        >  2:1977128/1‑101 (MQ=255)
                                                                                     gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       >  2:3627203/1‑101 (MQ=255)
                                                                                     gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       >  1:1793412/1‑101 (MQ=255)
                                                                                      cctccccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       <  1:2150842/98‑1 (MQ=255)
                                                                                       tGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCa     >  1:2363588/1‑101 (MQ=255)
                                                                                         cccccgC.CGAGGCGGTGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAgg   <  2:1709741/101‑1 (MQ=255)
                                                                                          nccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAgg   >  1:252081/2‑101 (MQ=255)
                                                                                           ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGCGCCCCGCAagg  >  2:712582/1‑101 (MQ=21)
                                                                                           ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  <  1:2587792/101‑1 (MQ=255)
                                                                                           ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  >  1:2354624/1‑101 (MQ=255)
                                                                                           ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  <  2:1646987/101‑1 (MQ=255)
                                                                                                |                                                                                               
GGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGGCGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740692‑1740880

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG