BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,745,454	+C	100%	intergenic (‑114/+654)	RCAP_rcc01613 ← / ← metH4	hypothetical protein/methionine synthase subunit B

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,745,454	1	.→C	100.0%	113.5	143	intergenic (‑114/+654)	RCAP_rcc01613/metH4	hypothetical protein/methionine synthase subunit B
Reads supporting (aligned to +/- strand): new base (73/37): ref base (2/31): total (75/68)
Fisher's exact test strand distribution p-value = 2.32e-10
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.09e-01

Only 100 of 175 total aligned reads displayed.


GCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.G.GCGCGAGCTTTGGCACCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAGGCGC  >  NC_014034/1745354‑1745549


  aGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.cc                                                                                               <  1:681257/101‑1 (MQ=255)


  aGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.cc                                                                                               <  1:1621248/101‑1 (MQ=255)


  aGCCCCCCTGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGGCGGC.GCGCGCGAG.GTTGGGAACT.C.A.cc                                                                                               >  2:1673285/1‑101 (MQ=255)


   gcgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.ccc                                                                                              <  2:966466/101‑1 (MQ=255)


    cgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.cccc                                                                                             <  1:1969230/101‑1 (MQ=255)


     gcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.ccccc                                                                                            >  1:188873/1‑101 (MQ=255)


       cccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.ccccccc                                                                                          <  2:3260469/101‑1 (MQ=255)


        ggggTGGGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.cccccccc                                                                                         <  1:801136/99‑1 (MQ=255)


          ggTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.cccccccccg                                                                                       >  2:4068192/1‑99 (MQ=255)


           gTGGGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.cccccccccgg                                                                                      <  1:3990773/101‑4 (MQ=255)


            tGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGGCGAC.GCGCGCGGGC.TTGGCACCT.C.A.ccccccccccgc                                                                                     >  1:3146635/1‑97 (MQ=255)


              cggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGgcgc                                                                                    >  1:1434486/1‑101 (MQ=255)


              cggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.ccccccccccgcgc                                                                                   >  2:1576692/1‑95 (MQ=255)


               ggggggcgggggTCGGCAGCCCGGCCGTCGGCTCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCa                                                                                   <  1:1453818/101‑1 (MQ=255)


               ggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.ccccccccccgcgca                                                                                  >  2:36793/1‑94 (MQ=255)


                gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCAc                                                                                  >  2:2207487/1‑101 (MQ=255)


                 ggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGGGGCGGTCGAC.GCCCGCGAGC.TTGGCACCT.C.C.CCCCCCCCCgcggcccc                                                                                >  2:2190562/1‑96 (MQ=255)


                 ggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCAcc                                                                                 >  1:4198282/1‑101 (MQ=255)


                    gcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGc                                                                              <  1:2625205/101‑1 (MQ=255)


                    gcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGc                                                                              <  1:2635506/101‑1 (MQ=255)


                    gcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGc                                                                              <  1:3000635/101‑1 (MQ=255)


                      gggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGcac                                                                            <  1:2948402/101‑1 (MQ=255)


                       ggggTCGACAGCCCGGCCGTCGGCTCGGCCAGCGGCAAGTCGATCGCGGCGGTCGCC.GCGCGCGAGC.TTGCCACCT.C.ACCCCCCCCCGGCGCACCGGcaca                                                                           <  2:4172679/101‑1 (MQ=255)


                          gTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCCGCGCCCCCGcacaagc                                                                        >  2:4432988/1‑98 (MQ=255)


                           tCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCCGCGCACCGGCACCGCCa                                                                       >  1:3208934/1‑101 (MQ=255)


                            cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCag                                                                      <  2:2661152/101‑1 (MQ=255)


                            cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCag                                                                      >  2:57113/1‑101 (MQ=255)


                            cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCag                                                                      <  2:3365620/101‑1 (MQ=255)


                             ggCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCaga                                                                     <  2:1095172/101‑1 (MQ=255)


                              gCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCagag                                                                    <  2:1434379/101‑1 (MQ=255)


                               ccgCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCagaga                                                                   <  1:4318984/99‑1 (MQ=255)


                               cAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCagaga                                                                   <  2:877264/101‑1 (MQ=255)


                               cAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCagaga                                                                   <  1:4152884/101‑1 (MQ=255)


                               cAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCagaga                                                                   <  2:196843/101‑1 (MQ=255)


                                aGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGGGAGC.TTGGCACCT.C.ACCCCCCCCCCGGGCCCCGGCACAACCagagcg                                                                  >  2:2040930/1‑99 (MQ=255)


                                aGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCCCCGGCACACCCagagag                                                                  >  2:1464288/1‑101 (MQ=255)


                                aGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCagagag                                                                  >  1:1329909/1‑101 (MQ=255)


                                   ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                               <  1:4144973/101‑1 (MQ=255)


                                   ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                               >  1:737900/1‑101 (MQ=255)


                                      gCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCgcggc                                                            >  2:2357536/1‑101 (MQ=255)


                                       ccGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCgcggcg                                                           <  1:13924/101‑1 (MQ=255)


                                        cGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGcggcgg                                                          >  1:2128332/1‑101 (MQ=255)


                                        cGTAGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGcggcgg                                                          <  1:3308264/101‑1 (MQ=255)


                                           cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCCCCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                       <  1:838775/101‑1 (MQ=255)


                                           cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGGGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                       >  1:324142/1‑101 (MQ=255)


                                           cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                       <  2:3510291/101‑1 (MQ=255)


                                           cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                       >  1:1453878/1‑101 (MQ=255)


                                           cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                       >  1:406697/1‑101 (MQ=255)


                                           cGGATCGGCCAGCGGAATGTCAATAGCGGCGGCCGGC.GCGCACGAGC.TTGGCCCCC.C.ACCCCCCCCCGGCGCACCGGGAGAGCCAGAGAGCGCGGCGGCCa                                                       <  2:3910256/101‑1 (MQ=255)


                                            ggATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCag                                                      >  2:3084524/1‑101 (MQ=255)


                                             gATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCaga                                                     <  1:18917/101‑1 (MQ=255)


                                              aTCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCagag                                                    >  2:4335075/1‑101 (MQ=255)


                                              aTCGGCCAGCGGCATGTCGATCGCGGCGGCCGACAC.GCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCCCCGGCACCGCCAGAGAGCGGGGCGGCCaggg                                                    >  2:1542601/1‑99 (MQ=255)


                                               tCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCCGCGCACCGGCACAGCCAGAGAGGGCGGCGGCCCGagc                                                   >  2:1152239/1‑101 (MQ=255)


                                                cGGCCAGCGGCATGTCGATCGCGGCGGATGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCCGagcc                                                  >  2:3272668/1‑101 (MQ=255)


                                                 ggCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCCCCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                 <  1:2833025/101‑1 (MQ=255)


                                                 ggCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                 <  2:4217900/101‑1 (MQ=255)


                                                  gCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                                >  1:540799/1‑101 (MQ=255)


                                                  gCCAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                                <  1:165117/101‑1 (MQ=255)


                                                    cAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccagcc                                              <  2:414562/101‑1 (MQ=255)


                                                    cAGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccagcc                                              >  2:2352171/1‑101 (MQ=255)


                                                     aGCGGCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCCCCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGccc                                             <  1:1589744/101‑1 (MQ=255)


                                                      gcgccattccGACCGGGGCGGTCGCC.GCGCGCGAGC.TGGGCCCCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGcccc                                            <  1:2291504/92‑1 (MQ=255)


                                                         gCATGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCgaga                                        >  1:2310845/1‑101 (MQ=255)


                                                            tGTCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCCGCCCCGAGAgg                                      >  2:3387008/1‑101 (MQ=255)


                                                              tCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                    <  1:3071935/101‑1 (MQ=255)


                                                              tCGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                    <  1:57126/101‑1 (MQ=255)


                                                               cGATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCa                                   <  2:2920392/101‑1 (MQ=255)


                                                                gATCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAg                                  <  1:2723163/101‑1 (MQ=255)


                                                                 gtCGCGGCGGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGt                                 <  2:2930594/100‑1 (MQ=255)


                                                                    gcggcgGTCGAC.GCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCaa                              <  1:1901802/101‑1 (MQ=255)


                                                                      ggcggTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAgg                            <  2:3428414/101‑1 (MQ=255)


                                                                       gcggTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACTGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGGCCCGCGAGGGCAGTGAAGGa                           >  2:1374523/1‑101 (MQ=255)


                                                                       gcggTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGa                           <  2:2942487/101‑1 (MQ=255)


                                                                        cggTCGAC.GCGCGCGAGC.TTGGCCCCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGaa                          <  1:1666766/101‑1 (MQ=255)


                                                                         ggTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGCGCCAGCCCCCGGGGGGCAGTCAAGGAAc                         >  2:332253/1‑101 (MQ=255)


                                                                         ggTCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                         >  1:3421579/1‑101 (MQ=255)


                                                                         ggTCGAC.GCGCGCGAGC.TTGGCACCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcg                        <  1:3619222/101‑1 (MQ=255)


                                                                          ggtggacgcgcgcGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                         <  2:1418941/92‑1 (MQ=255)


                                                                           tCGAC.GCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGCGGGCAGTCAAGGAAcgg                       >  2:1254423/1‑101 (MQ=255)


                                                                             cgacgcgcgcGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggc                      <  2:729081/95‑1 (MQ=255)


                                                                               acgcgcgcGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGACaggcgg                    >  2:3448804/5‑101 (MQ=255)


                                                                               acgcgcgcGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCACCGAGAGGGCAGTCAAGGAAcggcgg                    >  2:4364159/5‑101 (MQ=255)


                                                                                cgcgcgcGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCggg                   <  2:2648564/98‑1 (MQ=255)


                                                                                  cgcgcGAGC.TTGGCCCCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGcc                 <  1:1782152/100‑1 (MQ=255)


                                                                                    cgcgAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCg               >  2:796713/1‑101 (MQ=255)


                                                                                     gcgAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGgcccg              >  2:2117990/1‑99 (MQ=255)


                                                                                     gcgAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCgc              >  1:2504300/1‑100 (MQ=255)


                                                                                      cgAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCgca             >  2:3608704/1‑99 (MQ=255)


                                                                                         ggcttGGCCCCT.C.CCCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgc           <  2:119477/98‑1 (MQ=255)


                                                                                         agcttGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGGAgc           >  1:2367973/4‑101 (MQ=255)


                                                                                          gcttGGCCCCT.C.CCCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgcg          <  1:3352813/99‑1 (MQ=255)


                                                                                          gcttGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgcg          <  1:1152330/99‑1 (MQ=255)


                                                                                           cttGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgggc         >  1:969442/2‑101 (MQ=255)


                                                                                            ttGGCACCT.C.ACCCCCCCCCGGGGCCCCGGACCAGCCAGAGAGGGCGGCGGCCAGAGCCAACCCCGGGAGGGCAGTCAAGGAACGGCGGGGCCGAAGCGCa        >  2:1330500/1‑101 (MQ=255)


                                                                                             tGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCaa       <  2:1956050/101‑1 (MQ=255)


                                                                                              ggCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAg      <  2:1520679/101‑1 (MQ=255)


                                                                                              ggCACCT.CTC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAg      <  2:2807310/101‑1 (MQ=255)


                                                                                               gCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCCAAGCGCAAgg     >  2:503835/1‑101 (MQ=255)


                                                                                                  ccT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCCCAGCGCAAGgcgc  >  2:2850974/1‑101 (MQ=255)


GCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGAC.G.GCGCGAGCTTTGGCACCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAGGCGC  >  NC_014034/1745354‑1745549

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 22 ≤


ATCG

< 31 ≤


ATCG

< 40 ≤


ATCG