Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,751,840 +C 97.7% intergenic (+169/‑189) RCAP_rcc01619 → / → aldB Fis family transcriptional regulator/aldehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,751,8401.→C97.7% 2.1 ‑3.6 ‑0.1128intergenic (+169/‑189)RCAP_rcc01619/aldBFis family transcriptional regulator/aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (89/36):  ref base (0/3):  total (89/39)
Fisher's exact test strand distribution p-value = 2.68e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.08e-01

Only 100 of 163 total aligned reads displayed.

GATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCACGCGAGGGGGGCAGA.G.CC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGTCAGCCATAACT  >  NC_014034/1751740‑1751935
                                                                                                      |                                                                                                
gATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCaga.g                                                                                                   >  2:1577763/1‑101 (MQ=255)
aaTAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCaga.g                                                                                                   <  2:3957076/100‑1 (MQ=255)
 aTAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.c                                                                                                 >  2:2259618/1‑101 (MQ=255)
   atctgATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.c                                                                                              <  1:1863605/97‑1 (MQ=38)
    aaGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.cc                                                                                             >  1:2973192/1‑101 (MQ=255)
    aaGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.cc                                                                                             <  1:1544442/101‑1 (MQ=255)
     aGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.ccc                                                                                            <  1:3228208/101‑1 (MQ=255)
      ggATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.cccc                                                                                           >  1:3881899/1‑100 (MQ=255)
       gATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.cagat                                                                                          >  2:1863481/1‑97 (MQ=255)
        aTAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.ccccag                                                                                         >  1:3622452/1‑98 (MQ=255)
         tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.ccccagc                                                                                        >  2:1017024/1‑97 (MQ=255)
         tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.ccccaga                                                                                        <  2:24838/101‑5 (MQ=255)
         tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.ccccaga                                                                                        >  1:1746782/1‑97 (MQ=255)
         tAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.ccccaga                                                                                        >  2:3943594/1‑97 (MQ=255)
          agggCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.ccccagac                                                                                       >  2:3132211/3‑96 (MQ=37)
          aaGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.cc.ccccagac                                                                                       >  2:1530888/1‑96 (MQ=255)
           aGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGAcc                                                                                       >  2:1458527/1‑101 (MQ=38)
           aGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGAcc                                                                                       <  2:1191270/101‑1 (MQ=37)
            ggCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGAccc                                                                                      <  1:1520675/101‑1 (MQ=37)
              cgcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCg                                                                                    >  2:3906342/1‑101 (MQ=38)
               gcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGc                                                                                   <  1:536984/101‑1 (MQ=37)
               gcTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGc                                                                                   >  2:2030478/1‑101 (MQ=38)
                cTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGcc                                                                                  <  2:3594315/101‑1 (MQ=37)
                 tCCGCAAGGGCGCCTTCTTCCATATCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCt                                                                                 <  1:11481/101‑1 (MQ=37)
                  ccGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTg                                                                                <  1:3554618/101‑1 (MQ=37)
                  ccGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.G.CCTCCCAGACCCCGCCTg                                                                                <  1:3753525/101‑1 (MQ=38)
                    gCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCg                                                                              >  1:278012/1‑101 (MQ=38)
                    cgaaGGGGCCCTTCTTCCATTTCCCCCTTTCCCCTTGCCCCAAATTTCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCg                                                                              <  2:234882/99‑1 (MQ=25)
                     cAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCGGACCCCGCCTGCGa                                                                             >  1:3848474/1‑101 (MQ=37)
                        gggCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGAccc                                                                          >  2:2236829/1‑101 (MQ=38)
                          gcgcCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCcg                                                                        <  1:829130/101‑1 (MQ=37)
                           cgcCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCcgc                                                                       >  1:218304/1‑101 (MQ=38)
                              cttcttCCTTTCCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGcgcc                                                                    <  1:2681200/101‑1 (MQ=37)
                              cttcttCCATTTCCGCTTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGcgcc                                                                    <  2:4216948/101‑1 (MQ=37)
                              cttcttCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGcgcc                                                                    >  2:2362314/1‑101 (MQ=38)
                              cttcttCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGcgcc                                                                    >  1:1990899/1‑101 (MQ=38)
                              cttcttCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGcgcc                                                                    >  2:3165670/1‑101 (MQ=38)
                                tcttcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGcgccgc                                                                  >  1:572381/1‑101 (MQ=38)
                                  ttcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGTGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCt                                                                <  2:1943780/101‑1 (MQ=37)
                                   tcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCtt                                                               >  1:3452335/1‑101 (MQ=38)
                                   tcCATTTCCGCCTTTCACCTTGCCCCAAATATCCCCGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCtt                                                               <  2:1675462/101‑1 (MQ=37)
                                    ccATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCttt                                                              >  2:1409625/1‑101 (MQ=38)
                                     cATTTCCGCCTTGCCCCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTa                                                             <  1:1413949/101‑1 (MQ=37)
                                      ctttCCGCCTTTCCCCTTCCCCCAAATACCCCGGGGGGTGGGGCGCAGCCGGGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTAc                                                            <  1:83835/100‑1 (MQ=255)
                                      aTTTCAGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTAc                                                            >  2:4006068/1‑101 (MQ=37)
                                       tttCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACg                                                           >  1:1862642/1‑101 (MQ=38)
                                        ttCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGa                                                          >  2:1715286/1‑101 (MQ=38)
                                        ttCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGa                                                          >  2:2707391/1‑101 (MQ=38)
                                         tCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGaa                                                         >  2:1435499/1‑101 (MQ=38)
                                         tCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGaa                                                         >  1:2500263/1‑101 (MQ=38)
                                            gCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAAttt                                                      >  1:3497443/1‑101 (MQ=38)
                                            gCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAAttt                                                      >  2:399260/1‑101 (MQ=38)
                                            gCCTTTCACCTGGCCCCAAACATCCCGGGGGGTGAGTGGCAGCCGAGGGGGGCAGATC.CC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAAttt                                                      <  1:4229635/101‑1 (MQ=255)
                                             ccTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAAtttt                                                     >  2:821485/1‑101 (MQ=38)
                                              cTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCGTGCGACCCCGCGCCGCCTTTACGAATTTTg                                                    >  1:4400910/1‑101 (MQ=37)
                                              cTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTg                                                    >  1:2044473/1‑101 (MQ=38)
                                                ttCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCg                                                  >  1:1984730/1‑101 (MQ=37)
                                                   aCCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtg                                               <  1:493508/101‑1 (MQ=37)
                                                   aCCTTGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtg                                               >  1:380129/1‑101 (MQ=37)
                                                      ttGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtgctg                                            >  2:3398253/1‑101 (MQ=38)
                                                      tggCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtgctg                                            <  2:1839090/99‑1 (MQ=38)
                                                       tGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtgctgc                                           >  2:3053215/1‑101 (MQ=38)
                                                       tGCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGAtgctgc                                           <  1:220250/101‑1 (MQ=38)
                                                        gCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACTCCGCCCCGCCTTTACGAATTTTGCGCTgctgca                                          >  2:359163/1‑101 (MQ=37)
                                                        gCCCCAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgca                                          >  1:2155440/1‑101 (MQ=38)
                                                        gCCCCAAACATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgca                                          <  1:4011443/101‑1 (MQ=38)
                                                         ccccAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcag                                         >  1:1021667/1‑101 (MQ=38)
                                                          cccAAATATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagc                                        >  2:2945201/1‑101 (MQ=38)
                                                             aaaTATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagctgc                                     >  2:475464/1‑101 (MQ=38)
                                                             aaaTATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagctgc                                     >  2:945523/1‑101 (MQ=38)
                                                             aaaTATCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATgctgcagctgc                                     >  2:2182675/1‑101 (MQ=38)
                                                               atatCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCAc                                   >  1:3745835/1‑101 (MQ=38)
                                                                tatCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCAcc                                  >  1:2070119/1‑101 (MQ=38)
                                                                 atCCCGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCt                                 <  1:2008070/101‑1 (MQ=38)
                                                                    ccGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtc                              >  2:3547749/1‑101 (MQ=39)
                                                                     cGGGGGGTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtct                             >  2:2388388/1‑101 (MQ=255)
                                                                      ggggggTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtctc                            >  2:1079162/1‑101 (MQ=255)
                                                                      ggggggTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGtctc                            <  1:2769282/101‑1 (MQ=255)
                                                                          ggTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCAGCGCCGCCTCTACGAATTCTGCGATGCTGCAGCTGCAACTGTCTCGGCa                        >  2:2430766/1‑101 (MQ=255)
                                                                           gTGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAg                       >  1:2093455/1‑101 (MQ=255)
                                                                            tGAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGt                      >  2:2910361/1‑101 (MQ=255)
                                                                             gAGGCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTg                     >  1:646553/1‑101 (MQ=255)
                                                                               ggCGCAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGaa                   >  1:2645346/1‑101 (MQ=255)
                                                                               ggCGCAGCCGAGGGGGCCAGACC.CC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGaa                   <  2:3435277/101‑1 (MQ=255)
                                                                                gcgcAGCCGAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGaaa                  <  2:2869875/101‑1 (MQ=255)
                                                                                 cgcagccgAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAAc                 >  1:1367811/7‑101 (MQ=255)
                                                                                 cgcagccgAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAAc                 <  2:1157680/95‑1 (MQ=255)
                                                                                  gcagccgAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACa                >  2:2592241/6‑101 (MQ=255)
                                                                                   cagccgAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAg               >  2:625242/5‑101 (MQ=255)
                                                                                    agccgAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAgg              >  2:131733/4‑101 (MQ=255)
                                                                                    agccgAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAgg              >  2:1931999/4‑101 (MQ=255)
                                                                                    agccgAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAgg              >  1:1102512/4‑101 (MQ=255)
                                                                                      ccgAGGGGGGCAGACC.CC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGTc            <  1:1017704/100‑1 (MQ=255)
                                                                                       cgAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGTCa           >  2:719474/1‑101 (MQ=255)
                                                                                        gAGGGGGGCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGTCAg          >  1:1271337/1‑101 (MQ=255)
                                                                                          ggggggCAGA.GCCC.CCCAGCCCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGTCAGcc        <  1:4003529/101‑1 (MQ=255)
                                                                                           gggggCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGGCAGCCa       >  2:848921/1‑101 (MQ=255)
                                                                                              ggCAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAAAAGGTCAGCCATaa    >  1:4321882/1‑101 (MQ=255)
                                                                                                cAGA.GCCC.CCCAGCCCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGTCAGCCATAACt  <  1:4057145/101‑1 (MQ=255)
                                                                                                cAGA.GCCC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGTCAGCCATAACt  >  1:803262/1‑101 (MQ=255)
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GATAAAGGATAAGGCGCTCCGCAAGGGCGCCTTCTTCCATTTCCGCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAGGCGCACGCGAGGGGGGCAGA.G.CC.CCCAGACCCCGCCTGCGACCCCGCGCCGCCTTTACGAATTTTGCGATGCTGCAGCTGCACCTGTCTCGGCAGTGAAACAGGTCAGCCATAACT  >  NC_014034/1751740‑1751935

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG