Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,768,901 +G 2.1% intergenic (+28/‑28) lpxA → / → RCAP_rcc01636 acyl‑ACP‑‑UDP‑N‑acetylglucosamine O‑acyltransferase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,768,9011.→G 2.1% 4.6 ‑3.6 0.0186intergenic (+28/‑28)lpxA/RCAP_rcc01636acyl‑ACP‑‑UDP‑N‑acetylglucosamine O‑acyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (109/73):  total (109/77)
Fisher's exact test strand distribution p-value = 2.80e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

GAGCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGTGC  >  NC_014034/1768801‑1768995
                                                                                                      |                                                                                                
gagCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAAACGCGCTGCTC.Gc                                                                                                   >  2:2192450/1‑101 (MQ=255)
 agCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.Gc.c                                                                                                 >  2:572337/1‑101 (MQ=255)
     gccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..gggg                                                                                           >  1:1192955/1‑101 (MQ=255)
     gccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..gggg                                                                                           <  2:748569/101‑1 (MQ=255)
     gccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..gggg                                                                                           >  1:3556275/1‑101 (MQ=255)
        aCGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..ggggggg                                                                                        >  2:1911868/1‑101 (MQ=255)
        aCGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..ggggggg                                                                                        <  1:3394073/101‑1 (MQ=255)
        aCGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..ggggggg                                                                                        >  1:2038439/1‑101 (MQ=255)
         cggccGCGAAGTGCTTGATTTCATTCTTGGGCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTTAAACCGCGCTGATC.GC.C..gggggggg                                                                                       <  1:3990204/98‑2 (MQ=255)
          gTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..ggggggggc                                                                                      >  2:2369626/1‑99 (MQ=255)
          gTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCACAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..ggggggggc                                                                                      <  2:1035232/101‑3 (MQ=255)
             cgcgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCTCTGATC.GC.C..ggggggggcgcg                                                                                   <  1:3293614/101‑6 (MQ=255)
             cgcgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..ggggggggcgcg                                                                                   >  1:3936060/1‑96 (MQ=255)
               cgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCt                                                                                  >  1:3219017/1‑101 (MQ=255)
                gAAGTGCTTGATTTCATTCTTGGCCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTg                                                                                 <  2:1517913/101‑1 (MQ=255)
                 aaGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGc                                                                                >  1:3876572/1‑101 (MQ=255)
                 aaGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGc                                                                                <  1:4193782/101‑1 (MQ=255)
                 aaGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGc                                                                                >  2:3829295/1‑101 (MQ=255)
                  aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGcc                                                                               <  1:3595064/101‑1 (MQ=255)
                  aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGcc                                                                               <  2:3671394/101‑1 (MQ=255)
                  aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGcc                                                                               <  2:2078820/101‑1 (MQ=255)
                  aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGcc                                                                               >  1:308684/1‑101 (MQ=255)
                   gTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCg                                                                              <  2:675777/101‑1 (MQ=255)
                      cTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCgggg                                                                           >  2:2241670/1‑101 (MQ=255)
                       ttGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGGGGGGGGCGCGCTGCCgggg                                                                           <  2:2784715/101‑1 (MQ=255)
                         gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGgctg                                                                        <  1:2261544/101‑1 (MQ=255)
                         gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGgctg                                                                        >  2:524551/1‑101 (MQ=255)
                           tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGCCGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGgctgct                                                                      <  1:1670397/101‑1 (MQ=255)
                           tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGgctgct                                                                      <  1:1071024/101‑1 (MQ=255)
                            ttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCCGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCtt                                                                     <  1:4423533/101‑1 (MQ=255)
                              ctttCTTGGTCCCTCGGACCCCCCCCTCCTGCCGCGGCGTTGCGCGGGGGATGTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCtttt                                                                   <  1:1351302/99‑1 (MQ=255)
                              cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCtttt                                                                   <  1:3113651/101‑1 (MQ=255)
                               aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTg                                                                  <  1:2578303/101‑1 (MQ=255)
                               aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTg                                                                  >  2:1786755/1‑101 (MQ=255)
                               aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTg                                                                  >  2:3711428/1‑101 (MQ=255)
                               aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTg                                                                  >  1:1950320/1‑101 (MQ=255)
                                ttCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGc                                                                 <  2:3181152/101‑1 (MQ=255)
                                 tCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCa                                                                >  1:1822343/1‑101 (MQ=255)
                                 tCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCa                                                                >  2:2693357/1‑101 (MQ=255)
                                  cTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAg                                                               <  1:2498970/101‑1 (MQ=255)
                                   ttGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAgg                                                              <  1:2274810/101‑1 (MQ=255)
                                   ttGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAgg                                                              >  2:4032950/1‑101 (MQ=255)
                                     ggTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGcc                                                            >  2:4071614/1‑101 (MQ=255)
                                       tCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCcgc                                                          >  2:870887/1‑101 (MQ=255)
                                           tCGGACCGCCGCTTCCTGGCGCCGCGATGAGCGCGGGCTCCGAAACCGCGCTCATC.GC.CG.GGGGGGGCGCGCTGCCCGGGCTGCTTTTGCATGCCGCGCCg                                                      >  2:2006978/1‑101 (MQ=255)
                                             ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGa                                                    >  1:2158375/1‑101 (MQ=255)
                                             ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGa                                                    >  1:3050221/1‑101 (MQ=255)
                                               aCCGCAGCTTCCTGACGCGGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGaaa                                                  <  1:2678690/101‑1 (MQ=255)
                                               aCCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGaaa                                                  >  1:232414/1‑101 (MQ=255)
                                               aCCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGaaa                                                  <  1:1034012/101‑1 (MQ=255)
                                                 cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATg                                                >  2:3115807/1‑101 (MQ=255)
                                                 cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCGGCCGGGGCTGCTTTTGCCGGCCGCGCCGGAAATg                                                >  2:532730/1‑101 (MQ=255)
                                                    aGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTg                                             >  2:1280900/1‑101 (MQ=255)
                                                    aGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTg                                             >  1:531549/1‑101 (MQ=255)
                                                     gCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTgg                                            >  1:2897263/1‑101 (MQ=255)
                                                      cTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGt                                           >  1:1239241/1‑101 (MQ=255)
                                                      cTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGt                                           >  1:2449308/1‑101 (MQ=255)
                                                      cTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGt                                           <  1:57673/101‑1 (MQ=255)
                                                        tCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCt                                         <  2:2449151/101‑1 (MQ=255)
                                                        tCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCt                                         >  2:624271/1‑101 (MQ=255)
                                                        tCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCt                                         <  2:4265596/101‑1 (MQ=255)
                                                        tCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGCCCGCGCCGGAAATGCTGGTCt                                         <  1:2543449/101‑1 (MQ=255)
                                                         ccTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTg                                        <  2:4319766/101‑1 (MQ=255)
                                                          cTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgc                                       <  2:3308952/101‑1 (MQ=255)
                                                           tGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GCGG..GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcg                                      <  2:2158236/101‑1 (MQ=255)
                                                           tGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcg                                      <  2:3248793/101‑1 (MQ=255)
                                                            gACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcgc                                     <  2:1390285/101‑1 (MQ=255)
                                                            gACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcgc                                     >  1:1827686/1‑101 (MQ=255)
                                                               gccgcGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCt                                  <  2:1822224/101‑1 (MQ=255)
                                                                ccgcGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTg                                 <  2:1217368/101‑1 (MQ=255)
                                                                ccgcGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTg                                 <  2:3302636/101‑1 (MQ=255)
                                                                 cgcgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTgg                                <  1:2114456/101‑1 (MQ=255)
                                                                  gcgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGa                               >  1:959970/1‑101 (MQ=255)
                                                                   ctaTGAGCGCGGGATCTGAACCAGCGCTGATCGGA.G..GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                              <  2:196580/99‑1 (MQ=255)
                                                                   cgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                              >  1:1635288/1‑101 (MQ=255)
                                                                   cgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                              <  2:1662576/101‑1 (MQ=255)
                                                                    gATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGGGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                              >  2:2045618/1‑101 (MQ=255)
                                                                    gATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAg                             >  2:3981135/1‑101 (MQ=255)
                                                                    gATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAg                             <  1:53087/101‑1 (MQ=255)
                                                                       gAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTc                          >  2:2477550/1‑101 (MQ=255)
                                                                        aGCGCTGGATGTGAAACCGCGCCGGTC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGGAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCa                         <  1:2007107/101‑1 (MQ=255)
                                                                        aGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCa                         <  2:3218788/101‑1 (MQ=255)
                                                                        aGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCa                         <  1:4033207/101‑1 (MQ=255)
                                                                           gcgGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTAAGcc                      <  1:80060/101‑1 (MQ=255)
                                                                            cgGGATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGacc                     >  2:3749709/1‑101 (MQ=255)
                                                                             gggATCTGAAACCGCGCTGATC.GCGG..GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcg                    <  2:2536176/101‑1 (MQ=255)
                                                                             gggATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcg                    <  2:2624684/101‑1 (MQ=255)
                                                                              ggATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcgc                   >  1:1000552/1‑101 (MQ=255)
                                                                              ggATCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcgc                   >  2:130535/1‑101 (MQ=255)
                                                                                 tCTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGcc                >  1:3337612/1‑101 (MQ=255)
                                                                                  ccgAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCg               <  1:572405/99‑1 (MQ=255)
                                                                                  cTGAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCg               >  2:1848007/1‑101 (MQ=255)
                                                                                    gAAACCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCggg             >  2:611149/1‑101 (MQ=255)
                                                                                     aaaCCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGc            <  2:2292165/101‑1 (MQ=255)
                                                                                     aaaCCGCGCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGc            >  2:2121516/1‑101 (MQ=255)
                                                                                         cgcgCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGAt        <  2:1135174/101‑1 (MQ=255)
                                                                                         cgccCTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGAt        <  2:435997/101‑1 (MQ=255)
                                                                                             cTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGt    <  1:6267/101‑1 (MQ=255)
                                                                                             cTGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGt    >  2:456816/1‑101 (MQ=255)
                                                                                              tGATC.GC.CG.GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGtg   >  1:1650652/1‑101 (MQ=255)
                                                                                                      |                                                                                                
GAGCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..GGGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGTGC  >  NC_014034/1768801‑1768995

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG