Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,768,902 +G 97.0% intergenic (+29/‑27) lpxA → / → RCAP_rcc01636 acyl‑ACP‑‑UDP‑N‑acetylglucosamine O‑acyltransferase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,768,9021.→G97.0% 7.7 ‑4.2 ‑0.7165intergenic (+29/‑27)lpxA/RCAP_rcc01636acyl‑ACP‑‑UDP‑N‑acetylglucosamine O‑acyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (92/68):  ref base (0/5):  total (92/73)
Fisher's exact test strand distribution p-value = 1.57e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.92e-01

Only 100 of 200 total aligned reads displayed.

AGCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGTGC  >  NC_014034/1768802‑1768995
                                                                                                       |                                                                                               
agCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.Gc.c                                                                                                  >  2:572337/1‑101 (MQ=255)
agCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.Gc.c                                                                                                  <  2:134910/101‑1 (MQ=255)
   cgccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..g.gg                                                                                            >  2:1256107/1‑101 (MQ=255)
   cgccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..g.gg                                                                                            >  1:4029217/1‑101 (MQ=255)
    gccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..g.ggg                                                                                           >  1:1192955/1‑101 (MQ=255)
    gccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..g.ggg                                                                                           >  1:3556275/1‑101 (MQ=255)
       aCGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..g.gggggg                                                                                        <  1:3394073/101‑1 (MQ=255)
       aCGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..g.gggggg                                                                                        >  1:2038439/1‑101 (MQ=255)
         gTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..g.gggggggc                                                                                      >  2:981372/1‑99 (MQ=255)
         gTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..g.gggggggc                                                                                      >  2:2369626/1‑99 (MQ=255)
              cgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCt                                                                                  >  1:3219017/1‑101 (MQ=255)
              cgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCt                                                                                  >  1:3963929/1‑101 (MQ=255)
                aaGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGc                                                                                >  2:3829295/1‑101 (MQ=255)
                aaGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGc                                                                                >  1:4320035/1‑101 (MQ=255)
                 aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGcc                                                                               <  1:3595064/101‑1 (MQ=255)
                 aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGcc                                                                               >  1:308684/1‑101 (MQ=255)
                 aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGcc                                                                               <  2:2078820/101‑1 (MQ=255)
                 aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGcc                                                                               <  2:3671394/101‑1 (MQ=255)
                    gCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCggg                                                                            >  2:1033924/1‑101 (MQ=255)
                      ttGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGGGGGCGCGCTGCCgggg                                                                           <  2:2784715/101‑1 (MQ=255)
                       tGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGgct                                                                         >  2:1296096/1‑101 (MQ=255)
                        gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGgctg                                                                        <  1:2261544/101‑1 (MQ=255)
                        gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGgctg                                                                        >  2:524551/1‑101 (MQ=255)
                          tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGCCGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGgctgct                                                                      <  1:1670397/101‑1 (MQ=255)
                          tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGgctgct                                                                      >  1:746962/1‑101 (MQ=255)
                          tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGgctgct                                                                      <  1:1071024/101‑1 (MQ=255)
                             ctttCTTGGTCCCTCGGACCCCCCCCTCCTGCCGCGGCGTTGCGCGGGGGATGTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCtttt                                                                   <  1:1351302/99‑1 (MQ=255)
                             cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCtttt                                                                   >  1:566379/1‑101 (MQ=255)
                             cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCtttt                                                                   <  1:3113651/101‑1 (MQ=255)
                              aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTg                                                                  <  1:2578303/101‑1 (MQ=255)
                              aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTg                                                                  >  2:3711428/1‑101 (MQ=255)
                              aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTg                                                                  >  1:1950320/1‑101 (MQ=255)
                               ttCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGc                                                                 <  2:3181152/101‑1 (MQ=255)
                                tCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCa                                                                >  1:1822343/1‑101 (MQ=255)
                                tCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCa                                                                >  2:2693357/1‑101 (MQ=255)
                                 cTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAg                                                               <  1:2498970/101‑1 (MQ=255)
                                  ttGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAgg                                                              <  1:2274810/101‑1 (MQ=255)
                                  ttGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAgg                                                              >  2:4032950/1‑101 (MQ=255)
                                    ggTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGcc                                                            >  2:4071614/1‑101 (MQ=255)
                                          tCGGACCGCCGCTTCCTGGCGCCGCGATGAGCGCGGGCTCCGAAACCGCGCTCATC.GC.CG.G.GGGGGGCGCGCTGCCCGGGCTGCTTTTGCATGCCGCGCCg                                                      >  2:2006978/1‑101 (MQ=255)
                                            ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGa                                                    >  1:3050221/1‑101 (MQ=255)
                                            ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGa                                                    >  1:2158375/1‑101 (MQ=255)
                                            ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCATTTGCAGGCCGCGCCGGa                                                    >  1:451345/1‑101 (MQ=255)
                                              aCCGCAGCTTCCTGACGCGGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGaaa                                                  <  1:2678690/101‑1 (MQ=255)
                                              aCCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGaaa                                                  >  1:232414/1‑101 (MQ=255)
                                              aCCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGaaa                                                  <  1:1034012/101‑1 (MQ=255)
                                               ccGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAAt                                                 <  2:1759065/101‑1 (MQ=255)
                                                cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATg                                                >  2:3115807/1‑101 (MQ=255)
                                                cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATg                                                <  2:708872/101‑1 (MQ=255)
                                                cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCGGCCGGGGCTGCTTTTGCCGGCCGCGCCGGAAATg                                                >  2:532730/1‑101 (MQ=255)
                                                 gCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGc                                               >  1:3249025/1‑101 (MQ=255)
                                                  cAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCt                                              <  1:3769813/101‑1 (MQ=255)
                                                    gCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTgg                                            <  1:3897110/101‑1 (MQ=255)
                                                    gCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTgg                                            >  1:2897263/1‑101 (MQ=255)
                                                     cTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGt                                           >  1:1239241/1‑101 (MQ=255)
                                                     cTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGt                                           >  1:2449308/1‑101 (MQ=255)
                                                      ttCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTc                                          >  2:1650579/1‑101 (MQ=255)
                                                       tCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCt                                         <  2:4265596/101‑1 (MQ=255)
                                                       tCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCt                                         <  2:2449151/101‑1 (MQ=255)
                                                       tCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGCCCGCGCCGGAAATGCTGGTCt                                         <  1:2543449/101‑1 (MQ=255)
                                                        ccTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTg                                        <  2:4319766/101‑1 (MQ=255)
                                                         cTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgc                                       <  2:3308952/101‑1 (MQ=255)
                                                          tGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GCGG..G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcg                                      <  2:2158236/101‑1 (MQ=255)
                                                          tGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcg                                      <  2:3248793/101‑1 (MQ=255)
                                                          tGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcg                                      <  2:611450/101‑1 (MQ=255)
                                                           gACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcgc                                     >  1:1827686/1‑101 (MQ=255)
                                                              gccgcGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCt                                  <  2:1822224/101‑1 (MQ=255)
                                                               ccgcGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTg                                 <  2:3302636/101‑1 (MQ=255)
                                                                cgcgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTgg                                <  1:2114456/101‑1 (MQ=255)
                                                                  ctaTGAGCGCGGGATCTGAACCAGCGCTGATCGGA.G..G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                              <  2:196580/99‑1 (MQ=255)
                                                                  cgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                              >  1:1635288/1‑101 (MQ=255)
                                                                   gATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                              >  2:2045618/1‑101 (MQ=255)
                                                                   gATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAg                             >  2:3981135/1‑101 (MQ=255)
                                                                   gATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAg                             >  2:1141918/1‑101 (MQ=255)
                                                                      gAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTc                          >  2:2477550/1‑101 (MQ=255)
                                                                      gAGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTc                          >  2:104087/1‑101 (MQ=255)
                                                                       ggcgcGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCa                         <  2:81761/100‑1 (MQ=255)
                                                                       aGCGCTGGATGTGAAACCGCGCCGGTC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGGAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCa                         <  1:2007107/101‑1 (MQ=255)
                                                                       aGCGCGGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCa                         <  2:3218788/101‑1 (MQ=255)
                                                                           cgGGATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGacc                     >  2:3749709/1‑101 (MQ=255)
                                                                            gggATCTGAAACCGCGCTGATC.GCGG..G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcg                    <  2:2536176/101‑1 (MQ=255)
                                                                            gggATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcg                    <  2:2624684/101‑1 (MQ=255)
                                                                            gggATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcg                    <  2:1812924/101‑1 (MQ=255)
                                                                             ggATCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcgc                   >  1:1000552/1‑101 (MQ=255)
                                                                               aTCTGAACCCGCGCTGATC.TC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCgcgc                 <  1:573977/101‑1 (MQ=255)
                                                                                tCTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGcc                >  1:3337612/1‑101 (MQ=255)
                                                                                 cTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCg               <  1:603321/101‑1 (MQ=255)
                                                                                 cTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCg               >  2:1848007/1‑101 (MQ=255)
                                                                                 cTGAAACCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCg               <  2:2224384/101‑1 (MQ=255)
                                                                                    aaaCCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGc            >  2:2121516/1‑101 (MQ=255)
                                                                                    aaaCCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGc            <  2:2292165/101‑1 (MQ=255)
                                                                                      aCCGCGCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGcca          <  2:2257140/101‑3 (MQ=255)
                                                                                        cgccCTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGAt        <  2:435997/101‑1 (MQ=255)
                                                                                          tgatGATC.GC.C..GAGGGGGTCGCGCCTCAGTGGCTGCATTTGCGGGCCGGGCCGGAAATGTTGGACCGCGCGCTGGAAGGTCACCCCGCGCCGGGCGGATCg      <  2:1447619/98‑1 (MQ=255)
                                                                                           gcTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCgg     >  1:4133173/1‑101 (MQ=255)
                                                                                            cTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGt    <  1:53136/101‑1 (MQ=255)
                                                                                            cTGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGt    >  2:456816/1‑101 (MQ=255)
                                                                                             tGATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGtg   >  1:1650652/1‑101 (MQ=255)
                                                                                              gATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGtgc  >  2:6454/1‑101 (MQ=255)
                                                                                              gATC.GC.CG.G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGAtgc  >  1:932832/1‑101 (MQ=255)
                                                                                                       |                                                                                               
AGCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C..G.GGGGGGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGTGC  >  NC_014034/1768802‑1768995

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG