Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,370 +G 90.7% coding (442/744 nt) RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3701.→G90.7% 29.5 ‑3.8 ‑0.1118coding (442/744 nt)RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (50/57):  ref base (1/10):  total (51/67)
Fisher's exact test strand distribution p-value = 2.25e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.11e-01

Only 100 of 140 total aligned reads displayed.

TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GGCA.GGG.GCGGTGGTC.CC.CC.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462
                                                                                                       |                                                                                               
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGTCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTc                                                                                                  <  2:1653034/101‑1 (MQ=255)
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTc                                                                                                  >  2:2560609/1‑101 (MQ=255)
 gtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcg                                                                                                 >  2:3124405/1‑100 (MQ=255)
   gCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcg                                                                                               <  2:2576197/101‑4 (MQ=255)
   gCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcg                                                                                               <  2:1222999/101‑4 (MQ=255)
   gCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcg                                                                                               <  1:2443180/101‑4 (MQ=255)
   gCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcg                                                                                               <  1:2713541/101‑4 (MQ=255)
    cGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcgc                                                                                              <  2:623347/101‑5 (MQ=255)
    cGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGGGGTcgcgc                                                                                              >  1:609726/1‑97 (MQ=255)
     ggCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcgcg                                                                                             >  2:2317347/1‑96 (MQ=255)
         gCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcgcgcggg                                                                                         <  2:3780288/101‑10 (MQ=255)
         gCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcgcgcggg                                                                                         <  1:3124629/101‑10 (MQ=255)
          cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTcgcgcgcgggc                                                                                        <  1:177531/101‑11 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCttt                                                                                  <  2:494013/101‑1 (MQ=255)
               gCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGc                                                                                <  2:4306922/101‑1 (MQ=255)
                cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGcc                                                                               <  1:3672193/101‑1 (MQ=255)
                cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGcc                                                                               >  2:2204905/1‑101 (MQ=255)
                 gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCt                                                                              <  2:2503078/101‑1 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCtt                                                                             >  2:348336/1‑101 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCtt                                                                             >  2:3603087/1‑101 (MQ=255)
                   tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTg                                                                            >  2:696804/1‑101 (MQ=255)
                   tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTg                                                                            >  1:2436703/1‑101 (MQ=255)
                   tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTg                                                                            <  2:624719/101‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTgg                                                                           >  2:1303048/1‑101 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGt                                                                        <  1:3502752/101‑1 (MQ=255)
                        aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTc                                                                       <  1:2645551/101‑1 (MQ=255)
                        aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTc                                                                       <  1:332887/101‑1 (MQ=255)
                        aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTc                                                                       <  1:3909428/101‑1 (MQ=255)
                         ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCg                                                                      <  1:188598/101‑1 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGa                                                                     <  2:502883/101‑1 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGGCGa                                                                     >  1:2167565/1‑101 (MQ=255)
                           aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAg                                                                    <  1:19472/101‑1 (MQ=255)
                             cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgc                                                                  <  2:4006280/101‑1 (MQ=255)
                             cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgc                                                                  <  1:1817864/101‑1 (MQ=255)
                              tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgcg                                                                 >  1:1252309/1‑101 (MQ=255)
                              tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgcg                                                                 >  1:4385138/1‑101 (MQ=255)
                               ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgcgc                                                                >  1:4068504/1‑101 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCtt                                                              >  2:407463/1‑101 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  1:3033246/101‑1 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  2:758970/101‑1 (MQ=255)
                                    cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GCGCG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  2:2439398/101‑1 (MQ=255)
                                    cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GCGCG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  2:4017831/101‑1 (MQ=255)
                                     ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          >  2:1192476/1‑101 (MQ=255)
                                      gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         <  2:1459697/101‑1 (MQ=255)
                                      gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         >  2:1329232/1‑101 (MQ=255)
                                      gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GCGCG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         <  2:2866950/101‑1 (MQ=255)
                                         cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGATCGATGT.CGGGCAGGGG.GCGGTGGTC.GC.GCGCG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                      <  1:3801326/101‑1 (MQ=255)
                                           cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GC.GCGCG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  2:4334009/101‑1 (MQ=255)
                                            gcggcgGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGa                                                   >  2:3088838/1‑101 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:1102851/101‑1 (MQ=255)
                                              ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGGTg                                                 >  2:3748191/1‑101 (MQ=255)
                                               gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:4087687/101‑1 (MQ=255)
                                               gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTGTGCCTTGGGGTCGAGGCGCTTCCGGGCACGGATgc                                                >  2:1796125/1‑101 (MQ=255)
                                                cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               >  2:2892623/1‑101 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  1:575890/1‑101 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:1744235/1‑101 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             <  1:3623548/101‑1 (MQ=255)
                                                      tcgtcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                         <  1:407503/101‑1 (MQ=255)
                                                      acggcccgGCTTGGGGCTCAGTAGATAG.CGGGCA.GGGAGCGGTGGAC.CC.GC.GGCGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGGAACGATGCGCTGCtt                                         <  2:1563056/95‑1 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:1005157/1‑101 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        <  1:4405441/101‑1 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:2588100/1‑101 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  1:432728/1‑101 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  2:1853721/1‑101 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  2:3128591/1‑101 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  1:840454/1‑101 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGG.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     <  2:3021250/101‑1 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    >  1:4338261/1‑101 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:2798113/101‑1 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  1:1235897/101‑1 (MQ=255)
                                                             ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  2:3806012/1‑101 (MQ=255)
                                                             ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  <  1:1910892/101‑1 (MQ=255)
                                                             ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  1:1514710/1‑101 (MQ=255)
                                                               gtttGGGCGCTGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                <  2:4384866/100‑1 (MQ=255)
                                                               cTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                >  2:3842208/1‑101 (MQ=255)
                                                                tttGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               <  2:842451/101‑1 (MQ=255)
                                                                 ttGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              >  2:1703998/1‑101 (MQ=255)
                                                                 ttGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              >  1:2850076/1‑101 (MQ=255)
                                                                 ttGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              >  1:375015/1‑101 (MQ=255)
                                                                 ttGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              <  2:2066807/101‑1 (MQ=255)
                                                                    ggCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccgc                           >  2:3510195/1‑101 (MQ=255)
                                                                       gcgGTCGCTGTGG.GGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        <  2:953350/101‑1 (MQ=255)
                                                                         ggTCGATGT.CGGGGAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:3839224/101‑1 (MQ=255)
                                                                          gTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     <  1:3703315/101‑1 (MQ=255)
                                                                                 gtcgggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               <  2:3604391/92‑1 (MQ=255)
                                                                                 gtcgggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               <  2:1782373/92‑1 (MQ=255)
                                                                                    gggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  1:3954051/7‑101 (MQ=255)
                                                                                    gggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:3526385/7‑101 (MQ=255)
                                                                                    gggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:3055196/7‑101 (MQ=255)
                                                                                      gcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  1:4275097/97‑1 (MQ=255)
                                                                                      gcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  2:1005072/97‑1 (MQ=255)
                                                                                        agggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        <  1:3510436/99‑1 (MQ=255)
                                                                                        agggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        >  2:1596778/3‑101 (MQ=255)
                                                                                         gggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       >  2:2022841/2‑101 (MQ=255)
                                                                                         gggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       <  2:2509471/100‑1 (MQ=255)
                                                                                          ggg.gCGGTGGTCGCG.GG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  1:2644784/101‑1 (MQ=255)
                                                                                          ggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:1865525/101‑1 (MQ=255)
                                                                                          ggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      >  2:1915178/1‑101 (MQ=255)
                                                                                              gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   <  2:2587929/101‑1 (MQ=255)
                                                                                              gCGGTGGTC.GC.GCGCG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   >  2:786139/1‑101 (MQ=255)
                                                                                                       |                                                                                               
TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GGCA.GGG.GCGGTGGTC.CC.CC.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG