Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,805,344 +G 2.0% coding (1607/2094 nt) recG → ATP‑dependent DNA helicase RecG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,805,3441.→G 2.0% 4.5 ‑4.2 ‑0.4196coding (1607/2094 nt)recGATP‑dependent DNA helicase RecG
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (66/126):  total (70/126)
Fisher's exact test strand distribution p-value = 1.54e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.19e-01

Only 100 of 208 total aligned reads displayed.

TTCGCGCATCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCGAGACCTTCGGT  >  NC_014034/1805244‑1805444
                                                                                                     |                                                                                                      
ttCGCGCATCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGc                                                                                                         >  2:1310136/1‑101 (MQ=255)
ttCGCGCATCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGc                                                                                                         >  1:4392302/1‑101 (MQ=255)
     gcATCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.gggga                                                                                                   >  2:2770988/1‑99 (MQ=255)
     gcATCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGaa                                                                                                   <  1:1195390/101‑1 (MQ=255)
      cATCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGaaa                                                                                                  <  1:1864336/101‑1 (MQ=255)
      cATCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGaaa                                                                                                  <  2:4381134/101‑1 (MQ=255)
       aTCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGGCGCCGCGATGGCGGATTTTGTGGC.GGGAAAg                                                                                                 <  1:4026942/101‑1 (MQ=255)
       aTCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAg                                                                                                 <  2:3726667/101‑1 (MQ=255)
        tCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.a                                                                                               <  1:3981238/101‑1 (MQ=255)
          tGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.cc                                                                                            <  1:1338811/101‑1 (MQ=255)
          tGCCCATGGTGCTGGGCGAGGGCACCGTGGCCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.G.cc                                                                                            <  2:3659079/101‑1 (MQ=255)
           gcgcATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.ccc                                                                                           <  2:3369233/101‑1 (MQ=255)
            cgcATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGGAG.A.CACg                                                                                          >  1:821139/1‑101 (MQ=255)
              cATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCggg                                                                                        <  1:3410173/101‑1 (MQ=255)
                tGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.ACCggggc                                                                                      >  1:1114028/1‑99 (MQ=255)
                 ggTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGCGGGGAAAA.A.CCCGGGtc                                                                                      >  2:1168048/1‑100 (MQ=255)
                  gTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGGAG.A.CCCcggggct                                                                                    >  2:4064720/1‑97 (MQ=255)
                  gTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCt                                                                                    <  2:3171931/101‑1 (MQ=255)
                   tGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.CCCggggtgtg                                                                                   >  1:750988/1‑96 (MQ=255)
                    gCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCCGCTGCCGCCCGCCGAAAGGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAGA.A.GCCgggggtggg                                                                                  >  2:1988499/1‑95 (MQ=255)
                      tGGGCGGGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCtggtg                                                                                <  1:4084833/101‑1 (MQ=255)
                      tGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.ACCCGGGTCtggtg                                                                                >  2:12047/1‑101 (MQ=255)
                       gggCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCtggtgg                                                                               <  2:78795/101‑1 (MQ=255)
                        ggCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGc                                                                              <  2:2670071/101‑1 (MQ=255)
                         gCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGcg                                                                             <  2:1870341/101‑1 (MQ=255)
                         gCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGcg                                                                             <  2:2078923/101‑1 (MQ=255)
                          cGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGGAG.ACCCCGGGtttcgcggggg                                                                             >  1:4197030/1‑92 (MQ=255)
                           gAGGTCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGcgac                                                                           <  1:4254535/101‑1 (MQ=255)
                           gAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.CCCCGGGTGtggtgggggc                                                                           >  1:1393437/1‑97 (MQ=255)
                           gAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTAGC.GGGAAAG.A.CCCGGGTTCTGGTGGcgac                                                                           <  1:3673272/101‑1 (MQ=255)
                              ggCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCgacgac                                                                        <  1:2111660/101‑1 (MQ=255)
                                 cccGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGt                                                                     <  2:60659/100‑1 (MQ=255)
                                  ccGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.ACCGGGGTNTGGGGGGGACGACggag                                                                    >  2:1253736/1‑99 (MQ=255)
                                  ccGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTg                                                                    <  1:2462521/101‑1 (MQ=255)
                                   cGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.GGCCGGGTCTGGGGGCGACGACggggg                                                                   >  1:2009543/1‑98 (MQ=255)
                                   cGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGa                                                                   <  1:332629/101‑1 (MQ=255)
                                     tGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATc                                                                 <  1:3280360/101‑1 (MQ=255)
                                      gggCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.ACCGGGGTCTGGGGGGGGCGGCggggcgcg                                                                >  2:1684805/1‑95 (MQ=255)
                                      gggCCTGGTGAATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.CCCCGGGGCTCGTGGGGGCGACggggggcg                                                                >  2:1000040/1‑95 (MQ=255)
                                       ggCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAA.A.ACCGGGGGCTGGTGGGGAGGACGGGGATGGa                                                               >  1:1465849/1‑101 (MQ=255)
                                         ccTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.ACCGGGGTGTGGGGGGGGCGACGGGGGTCggcg                                                             >  1:3576746/1‑98 (MQ=255)
                                         ccTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAg                                                             <  2:2387649/101‑1 (MQ=255)
                                              tGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAA.A.CCCCGGGTCTGGTGGGGGCGGCGGGGGTCGGAGGgggg                                                        >  2:2369498/1‑101 (MQ=255)
                                               gCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.ACCCGGGTCTTGGGGCGGCGACGGGGGTGGGAGGggggg                                                       >  1:2904796/1‑101 (MQ=255)
                                                cATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGt                                                      <  1:4342724/101‑1 (MQ=255)
                                                cATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGt                                                      <  1:1697118/101‑1 (MQ=255)
                                                 aTGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAGAA.CCCGGGTT.TGGGGGGGGCGACGGGGGTCGAAGGggggggg                                                     >  1:3741184/1‑99 (MQ=255)
                                                  tGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGa                                                    <  1:3931112/101‑1 (MQ=255)
                                                   gggCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGCGGGGAAAA.A.CCCGGG.TCTGGGGGGGGCGACGGGGAGCGGAGGgggggggga                                                   >  1:3098166/1‑97 (MQ=255)
                                                   gggCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGaa                                                   <  2:1725969/101‑1 (MQ=255)
                                                     gCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACg                                                 <  2:3877973/101‑1 (MQ=255)
                                                      cAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.CCCGGGGTCTGTTGGGGGCGACGGGGGTCGAAGTGGGGGGGGACga                                                >  1:1213807/1‑100 (MQ=255)
                                                      cAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGt                                                <  1:3338180/101‑1 (MQ=255)
                                                           gccgccCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCa                                           <  1:2604404/101‑1 (MQ=255)
                                                           gccgccCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCa                                           <  2:3639005/101‑1 (MQ=255)
                                                           gccgccCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCa                                           <  1:1617667/101‑1 (MQ=255)
                                                           gccgccCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCa                                           <  2:3029442/101‑1 (MQ=255)
                                                            ccgcccgcCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCaa                                          <  2:3998951/101‑1 (MQ=255)
                                                            ccgcccgcCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GCGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCaa                                          <  2:4422050/101‑1 (MQ=255)
                                                             cgcccgccGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAAt                                         <  1:19271/101‑1 (MQ=255)
                                                             cgcccgccGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAAt                                         <  1:714388/101‑1 (MQ=255)
                                                                ccgccgAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGcc                                      <  2:3740931/101‑1 (MQ=255)
                                                                  gccgAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTc                                    <  2:4196884/101‑1 (MQ=255)
                                                                  gccgAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTc                                    <  1:456440/101‑1 (MQ=255)
                                                                   ccgAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCg                                   <  2:750908/101‑1 (MQ=255)
                                                                     gAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAA.A.CCCGGGGTCTGGGGGGGGCGGCGGGGGTCGAAGGGGGGGGGGAAGTGCCCCATACCTCgct                                 >  1:1734682/1‑99 (MQ=255)
                                                                     gAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAA.A.CCCCGGGTCTGGTGGCGGCGACGGGGGTCGGAGGGGGGGGGGACGTGCCCCATGCCTCggt                                 >  2:2548132/1‑99 (MQ=255)
                                                                        aaGGACGCCGCGATGGCGGATTTTGTGGC.GGGGAAG.A.ACCGGGGTCTGGGGGCGACGACGGGGATCGAAGAGGGGGGGGAAGTGCCCCATGCCTCGATCAt                              >  1:2846779/1‑101 (MQ=255)
                                                                        aaGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCAt                              <  2:2582356/101‑1 (MQ=255)
                                                                         aGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATg                             <  1:3387290/101‑1 (MQ=255)
                                                                         aGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAA.A.CCCGGGGTCTGGTGGGGGCGACGGTGGTAGCAGTGGGGGGGGAGGGGCCCCAAGCCTccctcacg                             >  2:1040836/1‑94 (MQ=255)
                                                                          ggACGCCGCGATGGCGGATTTTGTGGC.GGGGAAA.A.CCCCGGGTCTGGTGGCGGCGACGGGGATCGGAGGGGGGGGGGACGGGGCCCATGGCTCGATCCTgg                            >  2:663834/1‑101 (MQ=255)
                                                                          ggACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATgg                            <  2:3374288/101‑1 (MQ=255)
                                                                           gACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGt                           <  2:2427016/101‑1 (MQ=255)
                                                                           gACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGt                           <  2:3045002/101‑1 (MQ=255)
                                                                           gACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGGTCTGGGGGGGGCGACGGGGATCGGAGGGGGGGGGAACGTGCCCCAAGCCCCGATCATggg                           >  1:1494241/1‑100 (MQ=255)
                                                                              gccgcGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGAt                        <  2:3956376/101‑1 (MQ=255)
                                                                              gccgcGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGAt                        <  1:1756963/101‑1 (MQ=255)
                                                                              gccgcGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGAt                        <  1:1884249/101‑1 (MQ=255)
                                                                              gccgcGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGAt                        <  1:103963/101‑1 (MQ=255)
                                                                               ccgcGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATc                       <  1:1447937/101‑1 (MQ=255)
                                                                                tgcgATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCg                      <  2:540668/100‑1 (MQ=255)
                                                                                cgcgATGGCGGATTTTGTGGC.GGGAAAG.A.ACCCGGCTCTGGTGGCGAAGACGGGGGGCGAAGTGGGGGGGGAAGGGGCCAACACCTCGATCATTGGGAttg                      >  1:380803/1‑99 (MQ=255)
                                                                                 gcgATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGa                     <  1:1310233/101‑1 (MQ=255)
                                                                                   gATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGc                   <  1:3995114/101‑1 (MQ=255)
                                                                                     tGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCgg                 <  2:3201520/101‑1 (MQ=255)
                                                                                     tGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCgg                 <  1:933056/101‑1 (MQ=255)
                                                                                      ggCGGATTTTGTGGC.GGGAAAG.A.CCCGGGGTCTGGGGGGGGCGGCGGGGATCGAAGGGGGGGGGAACGTGGCCAATGCCTCGATCCTGGGGGTCGAGCggg                >  2:2536392/1‑101 (MQ=255)
                                                                                       gCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGc               <  2:3576498/101‑1 (MQ=255)
                                                                                       gCGGATTTTGTGGC.GGGAAAA.A.CCCGGGGTCTGGTGGGGGCGACGGGGATCGAAGGGGGGGGGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGc               >  2:2732340/1‑101 (MQ=255)
                                                                                        cGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGcc              <  1:1676579/101‑1 (MQ=255)
                                                                                        cGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGcc              <  2:1134057/101‑1 (MQ=255)
                                                                                           aTTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCgag           <  2:341538/101‑1 (MQ=255)
                                                                                           aTTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCgag           <  2:3097942/101‑1 (MQ=255)
                                                                                           aTTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCgag           <  1:556118/101‑1 (MQ=255)
                                                                                              ttGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCGAGAcc        <  1:2369560/101‑1 (MQ=255)
                                                                                              ttGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCGAGAcc        <  1:435901/101‑1 (MQ=255)
                                                                                                 tgGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCGAGACCTTc     <  1:1012896/101‑1 (MQ=255)
                                                                                                   gC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCGAGACCTTCgg   >  1:1951583/1‑101 (MQ=255)
                                                                                                    c.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCGAGACCTTCGGt  >  1:3240942/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
TTCGCGCATCTGCGCATGGTGCTGGGCGAGGGCACCGTGGGCCTGGTGCATGGGCAGCTGCCGCCCGCCGAAAAGGACGCCGCGATGGCGGATTTTGTGGC.GGGAAAG.A.CCCGGGTTCTGGTGGCGACGACGGTGATCGAAGTGGGGGTGAACGTGCCCAATGCCTCGATCATGGTGATCGAGCGGGCCGAGACCTTCGGT  >  NC_014034/1805244‑1805444

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG