BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,823,740	+C	100%	intergenic (+25/‑148)	RCAP_rcc01687 → / → RCAP_rcc01688	HK97 family phage major capsid protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,823,740	1	.→C	100.0%	39.1	139	intergenic (+25/‑148)	RCAP_rcc01687/RCAP_rcc01688	HK97 family phage major capsid protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (84/41): ref base (0/14): total (84/55)
Fisher's exact test strand distribution p-value = 7.43e-07
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.91e-01

Only 100 of 167 total aligned reads displayed.


CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCG  >  NC_014034/1823640‑1823833


  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.cc                                                                                              <  2:3288244/101‑1 (MQ=255)


  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGGGAAGGGGCCGGGGGC.A.A.cc                                                                                              >  1:1810136/1‑101 (MQ=255)


     cGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccg                                                                                           <  2:1205023/101‑3 (MQ=255)


      gAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccgg                                                                                          >  1:4379079/1‑98 (MQ=255)


      gAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccgg                                                                                          >  1:315466/1‑98 (MQ=255)


          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgc                                                                                      >  2:47789/1‑101 (MQ=255)


          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgc                                                                                      >  1:349305/1‑101 (MQ=255)


          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgc                                                                                      >  1:89620/1‑101 (MQ=255)


            gCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCTGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccg                                                                                    <  1:3985606/101‑1 (MQ=255)


            gCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccg                                                                                    >  2:3460178/1‑101 (MQ=255)


            gCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccg                                                                                    <  1:3202093/101‑1 (MQ=255)


            gCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccg                                                                                    <  2:1677822/101‑1 (MQ=255)


             cGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccgc                                                                                   >  2:1597228/1‑101 (MQ=255)


              gggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccgcc                                                                                  >  2:534594/1‑101 (MQ=255)


              gggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccgcc                                                                                  <  2:309865/101‑1 (MQ=255)


              gggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccgcc                                                                                  <  2:621453/101‑1 (MQ=255)


               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGG.A.ACCCC.GGCTGccgccg                                                                                 >  1:4287465/1‑101 (MQ=255)


               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGccgccg                                                                                 >  2:1457046/1‑101 (MQ=255)


               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGccgccg                                                                                 >  2:1572391/1‑101 (MQ=255)


               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGccgccg                                                                                 >  2:2811267/1‑101 (MQ=255)


                gTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCgg                                                                                >  1:996157/1‑101 (MQ=255)


                 tGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGt                                                                               >  1:3096104/1‑101 (MQ=255)


                  gggCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGtt                                                                              <  1:4195159/101‑1 (MQ=255)


                  gggCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGtt                                                                              >  2:3637191/1‑101 (MQ=255)


                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTc                                                                             <  2:3845545/101‑1 (MQ=255)


                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTc                                                                             <  1:1978969/101‑1 (MQ=255)


                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTc                                                                             >  2:1787853/1‑101 (MQ=255)


                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTc                                                                             <  1:534646/101‑1 (MQ=255)


                     cggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCAGTTCGc                                                                           >  1:759003/1‑101 (MQ=255)


                      ggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGcc                                                                          >  1:4353516/1‑101 (MQ=255)


                      ggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGcc                                                                          >  1:4144051/1‑101 (MQ=255)


                        cgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCgg                                                                        >  2:1249405/1‑101 (MQ=255)


                        cgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCgg                                                                        >  1:1008613/1‑101 (MQ=255)


                          aTGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTc                                                                      <  2:2108850/101‑1 (MQ=255)


                          aTGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTc                                                                      <  2:2637382/101‑1 (MQ=255)


                            gTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTcgg                                                                    <  1:3744189/101‑1 (MQ=255)


                             gtggCGATTCCGCGGCGAGCAGGCTCTTAAATTTTCCCGCCTCGTAACGGGCGGTGAAGGGGGGGGGGGC.C.C.CCCGGGCTGCCGCCGGTTCGCCGGTcggg                                                                   <  2:3047177/98‑1 (MQ=255)


                              cAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTcgggc                                                                  >  2:4351322/1‑101 (MQ=255)


                              cAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTcgggc                                                                  >  2:644364/1‑101 (MQ=255)


                               cgCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTcgggcg                                                                 <  2:2049637/100‑1 (MQ=255)


                               aGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTcgggcg                                                                 <  1:1727310/101‑1 (MQ=255)


                                gCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTcgggcgg                                                                >  1:1512063/1‑101 (MQ=255)


                                 ggATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTcgggcggg                                                               <  2:597332/100‑1 (MQ=255)


                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCgggcgggc                                                              >  1:567715/1‑101 (MQ=255)


                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGA.A.ACCCC.GGGTGCCGCCGGTTCGCCGGTCgggcgggc                                                              >  1:4172525/1‑101 (MQ=255)


                                    tttCGCGGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGgcgggcgc                                                            <  1:1056282/101‑1 (MQ=255)


                                      tCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGgcgggcgcgg                                                          <  2:2806066/101‑1 (MQ=255)


                                       cgccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGgcgggcgcggg                                                         >  1:605003/1‑101 (MQ=255)


                                       cgccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGgcgggcgcggg                                                         >  1:2469549/1‑101 (MQ=255)


                                        gccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGgcgggcgcgggc                                                        >  2:1940735/1‑101 (MQ=255)


                                         ccgcGATCAAGCTTTTGAAGTTTTCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCt                                                       <  1:2733944/101‑1 (MQ=255)


                                          cgcgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGCC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCtt                                                      <  2:1837185/101‑1 (MQ=255)


                                            cgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCAGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTcc                                                    <  2:3303315/101‑1 (MQ=255)


                                             gATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCg                                                   >  1:3506551/1‑101 (MQ=255)


                                             gATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCg                                                   >  1:2892109/1‑101 (MQ=255)


                                              aTCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCGGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCgg                                                  <  2:875319/101‑1 (MQ=255)


                                              aTCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCgg                                                  >  1:2681650/1‑101 (MQ=255)


                                              aTCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCgg                                                  >  1:2832414/1‑101 (MQ=255)


                                                cAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGcc                                                >  1:1901126/1‑101 (MQ=255)


                                                   gCTGTTGAAGTTTGCCGCCTCGTAGCGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCt                                             <  2:3938688/101‑1 (MQ=255)


                                                     tGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GCCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGc                                           >  2:2520035/1‑101 (MQ=255)


                                                      gTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAGGGGGCGGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGcc                                          <  2:1511958/101‑1 (MQ=255)


                                                      gTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGcc                                          >  1:3677878/1‑101 (MQ=255)


                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                         <  2:554376/101‑1 (MQ=255)


                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                        <  1:1621181/101‑1 (MQ=255)


                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                        >  2:686154/1‑101 (MQ=255)


                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGGGAAGGGGCCGGGGGC.A.ACCCC.GGCTGACGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCGGcccg                                        >  2:1369970/1‑99 (MQ=255)


                                                         gAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAg                                       >  1:2063234/1‑101 (MQ=255)


                                                         gAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAg                                       <  2:2849746/101‑1 (MQ=255)


                                                           aGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGGCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgct                                     >  2:3458641/1‑101 (MQ=255)


                                                           aGTTTCCCGCCGCGGAGGGGGGGGGGAAGGGGCGGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgct                                     <  1:870425/101‑1 (MQ=255)


                                                            gTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctg                                    >  1:87111/1‑101 (MQ=255)


                                                              ttGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgcc                                  >  2:4036143/1‑100 (MQ=255)


                                                                gccgccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctcc                                <  1:1168944/101‑1 (MQ=255)


                                                                gccgccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctcc                                <  2:2929385/101‑1 (MQ=255)


                                                                gccgccTCGTAACGGGCAGTGAAGGGTCCGGGGGC.A.A.CCCGGGATGCCCCCGGTTCGCCGGTCGGGCGGGCACGGGCTTCCGGCCGCTGCCTAGCTGctcc                                <  2:3680403/101‑1 (MQ=255)


                                                                  cgccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccct                              <  2:4353245/101‑1 (MQ=255)


                                                                   cccgcttagcGGGGGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGGGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctc                             <  1:4349546/92‑1 (MQ=255)


                                                                    ccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctcc                            <  2:3813558/101‑1 (MQ=255)


                                                                    ccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctcc                            >  1:2249219/1‑101 (MQ=255)


                                                                     cTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                           >  2:2104060/1‑101 (MQ=255)


                                                                     cTCGTAACGGGCAGTGAGGGGGTCGGGGGT.ACC.CCC.GGTTGCCTCCGGTTCGCTGGTCGGGTGGGCGCGGGCTTCCGGCCGCTTCCTAGCTGCTCCCtccg                           <  2:758605/101‑1 (MQ=255)


                                                                       cGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtc                         <  1:2787858/101‑1 (MQ=255)


                                                                         tAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtccg                       >  1:1283307/1‑101 (MQ=255)


                                                                         tAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtccg                       >  1:392031/1‑101 (MQ=255)


                                                                          aaCGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                      >  2:1420952/1‑101 (MQ=255)


                                                                             gggGGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTg                   <  2:2652637/101‑1 (MQ=255)


                                                                             gggCGGTGAGGGGGCCGAGGCCTA.C.CCC.GCCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTg                   <  2:758923/101‑1 (MQ=255)


                                                                              ggCGGTGAAGGGGCCGGGGGC.A.A.CCCGGGGTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTgg                  <  2:4237243/101‑1 (MQ=255)


                                                                              ggCGGTAAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTgg                  <  1:644432/101‑1 (MQ=255)


                                                                                   tGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTg             >  2:1193999/1‑101 (MQ=255)


                                                                                   ggAAGGGGCGGGGGGC.A.ACCCC.GGCTGCCGCCGTTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTg             <  1:81476/100‑1 (MQ=255)


                                                                                    gAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGc            >  1:4294498/1‑101 (MQ=255)


                                                                                     aaGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGGGCg           >  2:936830/1‑101 (MQ=255)


                                                                                      aGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCgg          <  2:2744602/101‑1 (MQ=255)


                                                                                       ggggCGGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCggg         <  1:4307442/101‑1 (MQ=255)


                                                                                        gggCCGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCgggg        >  2:2213548/1‑101 (MQ=255)


                                                                                           ccGGGGGC.ACC.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCt     <  2:1008528/101‑1 (MQ=255)


                                                                                            cGGGGGC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGGCTg    >  2:3386968/1‑101 (MQ=255)


                                                                                              ggggC.A.ACCCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTgcg  >  2:2591049/1‑101 (MQ=255)


CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.CCC.GGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCG  >  NC_014034/1823640‑1823833

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 24 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG