Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,867,831 +C 4.1% intergenic (‑233/+82) RCAP_rcc01729 ← / ← RCAP_rcc01730 pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,867,8311.→C 4.1% 2.8 ‑1.4 ‑0.174intergenic (‑233/+82)RCAP_rcc01729/RCAP_rcc01730pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand):  new base (0/3):  ref base (34/37):  total (34/40)
Fisher's exact test strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.99e-01

Only 100 of 167 total aligned reads displayed.

AGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CG.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCA  >  NC_014034/1867731‑1867927
                                                                                                      |                                                                                                     
aGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.g                                                                                                        <  1:2749059/101‑1 (MQ=255)
aGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.g                                                                                                        <  1:253778/101‑1 (MQ=255)
 ggAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.g.c                                                                                                      <  1:1127645/101‑1 (MQ=255)
 ggAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.g.c                                                                                                      <  2:821429/101‑1 (MQ=255)
  gAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCCGCGGCGCGGAA.a.cg                                                                                                     >  1:3874400/1‑101 (MQ=38)
    aaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.cg.cc                                                                                                  <  2:867126/101‑1 (MQ=255)
        cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCGGCGGCGCGCAA.G.cg.cccgcc                                                                                              >  2:614293/1‑97 (MQ=25)
        cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGGAA.A.cg.cccgcc                                                                                              >  1:459391/1‑97 (MQ=37)
         aCTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACCCCCAGCCCCCCCGGCGCCAAA.C.Cg.ccccccc                                                                                             >  2:1414603/1‑99 (MQ=37)
           tGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGCAA.G.cc.cccgccacg                                                                                           >  2:2931765/1‑94 (MQ=37)
           tGCTTTCCCCCGCCCGTGTCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGCGAGGGGCCAGCGCCCACCCCCGGCGGCGCGAAA.G.CGCCCGCCATg                                                                                            <  2:3416217/101‑1 (MQ=25)
             cTTTTCCCCGCCGGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCGCGGCGCGGAA.G.cg.cccgccccggg                                                                                         >  1:2423619/1‑92 (MQ=255)
              ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATgggc                                                                                         <  2:1872053/101‑1 (MQ=255)
                 tCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATgggcggg                                                                                      <  2:4382347/101‑1 (MQ=255)
                  ccccGCCGGTGTCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGGGCGAAA.G.CGCCCGCCATgggcgggg                                                                                     <  1:3502926/101‑1 (MQ=21)
                  ccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATgggcagat                                                                                     >  1:1383977/1‑97 (MQ=255)
                  ccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCCTgggccggg                                                                                     >  2:1122110/1‑101 (MQ=25)
                   cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATgggcggggc                                                                                    <  2:2695052/101‑1 (MQ=255)
                   cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATgggcggggc                                                                                    <  1:4054189/101‑1 (MQ=255)
                    ccgcccgTGTCGCGACAGCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATgggcggggcg                                                                                   <  1:3287926/101‑1 (MQ=255)
                      gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCGGCGGCGCGGAA.G.CGCCCGCCCCGGgcggggcggg                                                                                 >  2:2920905/1‑101 (MQ=18)
                      ccccgTGTCGCGACAGCGTTGAAAAGCCCCCACCCCCAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGgcggggcggg                                                                                 <  2:1577350/100‑1 (MQ=18)
                       cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGgggcgggc                                                                                <  2:3978820/101‑1 (MQ=255)
                        ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGggcgggcg                                                                               <  2:3471697/101‑1 (MQ=255)
                        ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGggcgggcg                                                                               <  2:3241838/101‑1 (MQ=255)
                        ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGggcgggcg                                                                               <  2:2112957/101‑1 (MQ=255)
                        ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGAGCCCAGCCCCCGCGGCGCGAAA.A.CGCCCGCCACCGGCGGggggggcg                                                                               >  2:3001408/1‑101 (MQ=21)
                        ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCGCGGCGCGAAA.G.CGCCCGCCCTCGgcgggggggggg                                                                               >  2:4154080/1‑99 (MQ=18)
                        ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCGCGGCGCCAAA.G.CGCCCCCCCCGGgcgggggggggg                                                                               >  2:1156526/1‑99 (MQ=18)
                        ccgTGTCCCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGCCAAGCGCCCGGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGggcgggcg                                                                               <  2:441852/101‑1 (MQ=18)
                         cgggtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCCGGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGgcg.c                                                                             <  2:2880125/98‑1 (MQ=17)
                         cgTGTCGCGACGGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CCCCCGCCATGGGCGGGGCGGgcg.c                                                                             <  1:4309788/101‑1 (MQ=18)
                         cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCACCCCCCGCCGCGCGAAA.G.CGCCCCCCCAGGGGGGGGGGGgcg.c                                                                             >  1:1514467/1‑101 (MQ=25)
                         cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGCAA.G.CGCCCGCCCCGGGGGGGGGGGggg.c                                                                             >  1:2062653/1‑101 (MQ=25)
                         cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCACCCCCGGCGGCGCGAAA.A.CGCCCGCCCTGGGCGGGGGGGggg.c                                                                             >  1:66619/1‑101 (MQ=21)
                          gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGGGGCGCGAAA.A.CGCCCGCCACGGGCGGGGCGGggg.cc                                                                            >  1:2831902/1‑100 (MQ=18)
                           tgtCGCGACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTg                                                                           <  2:3384249/101‑1 (MQ=18)
                           tgccgcgACAGCGTTGCAAAGCCCCCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTg                                                                           <  2:2543825/98‑1 (MQ=18)
                             tCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGc                                                                         <  2:3819974/101‑1 (MQ=18)
                              cgcgACAGCGTTGCAAAGACCGCATCCCCAACCGCGCGAGAGGCCAGCCCCCACCCCCGGCGGCGCGAAA.C.CGCCCGCCATGGGCGGGGCGGGCG.CTGGcc                                                                        <  1:1413846/101‑1 (MQ=18)
                                cgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGTCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CCGGCC.G.a                                                                    >  1:1349332/1‑101 (MQ=18)
                                 gACAGCGTTGCAAAGCCCCCATCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.g                                                                  <  1:1273156/101‑1 (MQ=17)
                                   cAGGGTTGCAAAGACCGTATCCCCAACCGCGCGAGAGGCCACCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.ggg                                                                <  1:3694959/101‑1 (MQ=17)
                                   cAGCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.ggg                                                                <  2:321140/101‑1 (MQ=255)
                                      cGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCCCCCTGGGCGGGGCGGGGG.CCGGGC.G.G.ggggcc                                                             >  1:270516/1‑98 (MQ=17)
                                       nnnnaannGACCGCACCNCCAGGCGCGCGAGAGGCCAGCGCCCGGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCtt                                                            <  2:1837733/96‑1 (MQ=14)
                                       gtggCAAAGCCCGCATCCCCAAGCGGGGGGAAGGCAAGCGCCCAGCCCCGGCGGCCCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCtt                                                            <  1:4189876/98‑1 (MQ=14)
                                         tGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGAAA.A.CGCCCGCCCATGGCGGGGGGGGGG.CCTGCC.G.ACGGGCCttt                                                           >  2:2803609/1‑101 (MQ=12)
                                          gCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGAAA.G.CGCCCCCCCTGGGCGGGGGGGGGG.CGGGGC.GCG.GGGCCTTTg                                                          >  1:52126/1‑101 (MQ=12)
                                           cAAAGACCGCATCCCCAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCTGGGCGGGCG.CTGGCC.G.A.GGGCCTTTggg                                                        <  2:2749621/101‑1 (MQ=17)
                                           cAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCCTGGGCGGGGCGGGGGCCGGGCG.G.G.GGGCCTTTgg                                                         >  2:2016471/1‑101 (MQ=17)
                                           cAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGAAA.C.AGCCCGCCATGGGCGGGGGGGGGG.CTGGCC.G.A.GGGCCCCTggg                                                        >  2:307907/1‑101 (MQ=17)
                                            aaaGACCGCACCCCCAGGCGCGCGAGGGGCCAGCCCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTgggg                                                       <  1:2413693/101‑1 (MQ=17)
                                              gcgcagCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CCGGCC.G.AGGGGCCTTTGGGGc                                                      >  2:4117732/6‑101 (MQ=17)
                                               gcccGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCGGGCGGCGCGAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGccc                                                    <  1:3360931/99‑1 (MQ=17)
                                               gACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCGCCGGGCC.C.G.GGGCCTTTGGGGcc                                                     >  2:3668871/1‑101 (MQ=17)
                                                cccGCATCCCCAAGCGCGCGAGAGGCCGGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGcccc                                                   <  2:399165/100‑1 (MQ=255)
                                                 cctcacccccATGCGCGCGAGAGCCCCGCCCCCACCCCGGGCGTCGCGAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGccccc                                                  <  2:3127838/95‑1 (MQ=14)
                                                 ccgcccccccAGGCGCGGGAGGGCCCGCCCCCCACCCCCGGCGGCGCAAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGccccc                                                  <  2:1514362/95‑1 (MQ=9)
                                                 ccccacccccAGGGGCGGGGGAGGCCGGCCCCCAGCCCGGGCGGCGCGAAACC.CC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGccccc                                                  <  1:906599/95‑1 (MQ=9)
                                                   gCACCCCAAGGCGGGGGAGGGGCGAGCGCCCAGCCCCGGCGGCGCGAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCcgg                                                <  2:1056364/101‑1 (MQ=14)
                                                   ccacccccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCcgg                                                <  2:1172855/97‑1 (MQ=255)
                                                     cccccggaGCGGGCGAGAGCCCGGCCCCCCGCCCCGGCGGCGCGAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCcggcg                                              <  1:111301/94‑1 (MQ=11)
                                                       ccccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CCGGGC.G.G.GGGCCCTTGGGGCCCCcggcggg                                            >  1:299475/1‑100 (MQ=17)
                                                         ccAAGCGCGCGGGAGGCCTGCGTCCCGCCCCGGCGGCGCGAAT.G.CGCCCGCCATGGGCGGGGCGGGCG.CCGGCC.G.G.GGGGCTCCGGGTccctggggggccc                                          >  1:2342450/1‑91 (MQ=17)
                                                         ccAAGCGCGCGGGAAGCGAGCCCCCAGCCCCGGAGGCGCGAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCa                                          <  1:4421062/101‑1 (MQ=17)
                                                         ccAAGCGCGCGAGGGGCCGGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCa                                          <  1:2222683/101‑1 (MQ=17)
                                                          cacgcgcgcGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAg                                         <  2:66606/98‑1 (MQ=17)
                                                          cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CCGGCC.G.AGGGGCCTTTGGGG.CCCcggcgggccg                                         >  2:1358345/1‑97 (MQ=14)
                                                          cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CCGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAg                                         >  1:2996507/1‑101 (MQ=255)
                                                           aaGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CCGGGC.G.A.GGGCCTTTGGGGGCCCcggcgggccgg                                        >  2:1246212/1‑96 (MQ=17)
                                                           aaGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CCGGCC.G.C.GGGGCCTTGGGGCCCCCGGGGGGCAgg                                        >  2:3490078/1‑101 (MQ=17)
                                                               gcgcgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCCTGGGCGGGGCGGGGG.CTGGCC.G.A.GGGCCCTTGGGGGCCCCCGCGGGCCgggggg                                    >  1:4213999/1‑100 (MQ=14)
                                                               cgcggacagGCCAGCACCCGCCCCGGGCGTCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGa                                    <  2:1349235/94‑1 (MQ=14)
                                                                cgcgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGGC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAggggggt                                   >  2:4170409/1‑99 (MQ=255)
                                                                 gcgGGAGCCCGGCCCCCGGCCCCGGCGGCGAGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATc                                  <  2:11345/101‑1 (MQ=14)
                                                                 gcgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGGC.G.G.GGGCCTTTGGGGCCCCCGGCGGGCCGGGGGGTc                                  >  1:1883413/1‑101 (MQ=17)
                                                                   aggagCCCGGCGCCCGGCCCCGGCGGGGCGAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                <  1:614361/99‑1 (MQ=17)
                                                                    agagGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCCTGGGCGGGGCGGGGG.GCGGCCGG.G.GGGCCTTTGGGG.CCCCGGCGGGCCGGGGGGTCTGt                               >  1:21763/1‑101 (MQ=2)
                                                                     gagGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGtt                              >  1:1888031/1‑101 (MQ=255)
                                                                     gagGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCCTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              >  2:3568901/1‑101 (MQ=255)
                                                                          cAGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                         >  1:4192310/1‑101 (MQ=255)
                                                                          cAGCCCCCACCCCCGGCGGCGCGAAG.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                         <  1:4154341/101‑1 (MQ=17)
                                                                           ggcgcCCGGCCCGGGGGGCGAGAAG.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTACGCAt                        <  2:1646133/100‑1 (MQ=14)
                                                                           ggcccccCGCCCCGGCGGCGGGAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        <  1:1706524/97‑1 (MQ=17)
                                                                           aGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTGGGCAt                        >  1:1901358/1‑101 (MQ=17)
                                                                           aGCGCCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        >  2:2468235/1‑101 (MQ=255)
                                                                              gcCCAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGc                     >  2:2311863/1‑101 (MQ=255)
                                                                               cccccccccGGCGGCGCAAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                    <  1:3301704/96‑1 (MQ=18)
                                                                               cccAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                    >  1:3917631/1‑101 (MQ=255)
                                                                                 cAGCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCATTGGGGCCCCCCGCGGGCAGGGGGATCTGGTGCGCAGAGCcgc                  >  2:1505817/1‑101 (MQ=17)
                                                                                   gCCCCGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGt                <  2:3109397/101‑1 (MQ=255)
                                                                                   cccccGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGt                <  1:1937212/100‑1 (MQ=255)
                                                                                    cccgGGGGGCGCGAAG.C.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc               <  1:3412860/101‑1 (MQ=18)
                                                                                    ccccGGCGGCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc               >  1:1516262/1‑101 (MQ=255)
                                                                                     cccGGCGGCGCCAAA.GCCC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa              <  1:2010630/101‑1 (MQ=21)
                                                                                       cggcggCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCacc            >  2:4208442/1‑99 (MQ=255)
                                                                                       cggcggCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgc            <  2:3981423/101‑1 (MQ=255)
                                                                                       cggcggCGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGGCAGGGGGGTCTGTTGCGCATGGCCGCGGCAgc            >  1:3066013/1‑101 (MQ=18)
                                                                                        ggcggcGCGAAA.G.CGCCCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcg           <  1:4163684/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
AGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAA.G.CG.CCGCCATGGGCGGGGCGGGCG.CTGGCC.G.A.GGGCCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCA  >  NC_014034/1867731‑1867927

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG