BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,948,851	+G	93.2%	coding (1069/2472 nt)	quiP ←	acyl‑homoserine‑lactone acylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,948,851	1	.→G	93.2%	36.9 ‑0.9 ‑0.4	205	coding (1069/2472 nt)	quiP	acyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand): new base (83/108): ref base (8/6): total (91/114)
Fisher's exact test strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.38e-01

Only 100 of 214 total aligned reads displayed.


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GC.CC..A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943


    ccGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.cc                                                                                              >  2:1359228/1‑101 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.ccca                                                                                            <  2:2873780/101‑3 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.ccca                                                                                            >  1:3716895/1‑99 (MQ=255)


      ccgatctgcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.ccca                                                                                            <  2:2493776/94‑3 (MQ=255)


       aCGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.cccaa                                                                                           <  1:924855/101‑4 (MQ=255)


          gcagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.cccaaccc                                                                                        <  1:1407193/101‑7 (MQ=255)


             gcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCg                                                                                    >  1:783395/1‑101 (MQ=255)


              ccccGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCgg                                                                                   <  1:1510325/99‑1 (MQ=255)


              caCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCgg                                                                                   <  1:2205966/101‑1 (MQ=255)


               gacGGGCCGTTTTCGGTCAAGCGCAGGGTGAGGGTGATCGGCGCGGCAACCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCggg                                                                                  <  2:2939222/99‑1 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCt                                                                               <  1:3480729/101‑1 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCt                                                                               >  2:3180845/1‑101 (MQ=255)


                   ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTg                                                                              <  2:4176078/101‑1 (MQ=255)


                    ggcGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGc                                                                             <  2:2245025/99‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGc                                                                             <  2:3291545/101‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGc                                                                             <  2:3028524/101‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGc                                                                             <  1:4359671/101‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGc                                                                             <  1:2782216/101‑1 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGt                                                                          <  2:4345180/101‑1 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGt                                                                          <  1:1224130/101‑1 (MQ=255)


                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTa                                                                         <  2:1521019/101‑1 (MQ=255)


                         tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTag                                                                        >  1:1368728/1‑100 (MQ=255)


                             ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTagcgct                                                                    >  2:1919809/1‑96 (MQ=255)


                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTc                                                                   <  2:4144784/101‑1 (MQ=255)


                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GG.CCAAA.CCCTCGGGGCTGCGGTAGCGCTc                                                                   <  1:1944085/101‑1 (MQ=255)


                               tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCg                                                                  >  2:2434235/1‑101 (MQ=255)


                                ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTTGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCgg                                                                 <  2:848781/101‑1 (MQ=255)


                                ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCgg                                                                 <  1:2921035/101‑1 (MQ=255)


                                 cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCggg                                                                >  2:406687/1‑101 (MQ=255)


                                  aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGc                                                               <  1:2293442/101‑1 (MQ=255)


                                   gcgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCg                                                              >  2:4151305/1‑101 (MQ=255)


                                   gcgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCg                                                              >  1:4156604/1‑101 (MQ=255)


                                    cgcAGGGTGAGGGTGATCGTCGCGGCATCCTTGACCGTGACCATTACCTTGCGGGGGGTGAACTC.GG.CCTAA.CCCTCGGTGTTGCGGAAGCGCGCGGGCGt                                                             >  2:2134061/1‑101 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            >  1:2968414/1‑101 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            >  1:427348/1‑101 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            <  2:1569733/101‑1 (MQ=255)


                                      cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           <  2:1410770/101‑1 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  1:2529431/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  1:166193/1‑101 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGGCCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  2:3567780/101‑1 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  1:3500986/101‑1 (MQ=255)


                                         tccgatcttgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CC..ACCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCg                                                        <  2:494194/93‑1 (MQ=255)


                                         ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCg                                                        <  1:2437081/101‑1 (MQ=255)


                                             agggtgaTCGGCGCGGCTTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    >  1:3142601/1‑101 (MQ=255)


                                                gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                 <  1:2507428/101‑1 (MQ=255)


                                                gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                 >  2:3065053/1‑101 (MQ=255)


                                                 tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGCTCAAGt                                                >  1:30559/1‑101 (MQ=255)


                                                 tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGt                                                >  1:4104462/1‑101 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               <  1:656420/101‑1 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               <  1:169331/101‑1 (MQ=255)


                                                    tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                             <  2:2522341/101‑1 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  2:169399/101‑1 (MQ=255)


                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  1:338577/101‑1 (MQ=255)


                                                        cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         >  1:994266/1‑101 (MQ=255)


                                                        cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         <  1:3995790/101‑1 (MQ=255)


                                                        cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         >  1:486518/1‑101 (MQ=255)


                                                         gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                        >  1:3931986/1‑101 (MQ=255)


                                                          ggggaTCCTTGACGGTGGCGATCACCTTGTGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCTCTCGGGTTTTTTCGGATCAAGTTCCTcgacg                                       <  2:1839321/97‑1 (MQ=255)


                                                          cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                       <  2:1080563/101‑1 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     <  2:2729591/101‑1 (MQ=255)


                                                             cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    <  1:3469182/101‑1 (MQ=255)


                                                             cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    >  2:3331877/1‑101 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   >  2:1173500/1‑101 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGGGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   >  2:3904810/1‑101 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  1:954308/1‑101 (MQ=255)


                                                                ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                 >  2:2409361/1‑101 (MQ=255)


                                                                ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                 >  2:549895/1‑101 (MQ=255)


                                                                ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                 <  1:2684924/101‑1 (MQ=255)


                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                >  2:3678225/1‑101 (MQ=255)


                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:2285023/1‑101 (MQ=255)


                                                                   tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                              >  1:2651552/1‑101 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:2274903/101‑1 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:1064483/101‑1 (MQ=255)


                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:2971770/101‑1 (MQ=255)


                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  1:1467703/101‑1 (MQ=255)


                                                                       cGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                          <  2:282433/101‑1 (MQ=255)


                                                                       cGTGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                          <  1:4151566/101‑1 (MQ=255)


                                                                         tGACGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        <  1:3662947/101‑1 (MQ=255)


                                                                           aCGATCGCCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATc                      <  1:1621452/101‑1 (MQ=255)


                                                                           aCGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATc                      >  2:3469829/1‑101 (MQ=255)


                                                                            cGATCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  1:626955/101‑1 (MQ=255)


                                                                             gATCACCTTGCGGGTGGTGAAATCGGCACA..A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    >  2:4431744/1‑101 (MQ=255)


                                                                              aTCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                   <  2:641478/101‑1 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  <  2:77324/101‑1 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  <  1:1107560/101‑1 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  <  1:406727/101‑1 (MQ=255)


                                                                                  ctttGCGGGTGGTGAACTCGGC.CCA.A.ACCTCGGGGCTGCGGTAGCGCTAGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               <  1:1804294/99‑1 (MQ=255)


                                                                                  ccTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               <  1:3692014/101‑1 (MQ=255)


                                                                                  ccTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               <  2:3932342/101‑1 (MQ=255)


                                                                                  ccTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               <  1:1993574/101‑1 (MQ=255)


                                                                                   cTTGCGGGTGGTGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg              <  2:4284078/101‑1 (MQ=255)


                                                                                         ggtggtGAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg        <  1:550694/101‑1 (MQ=255)


                                                                                          gtggtgAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGt       >  1:456382/1‑101 (MQ=255)


                                                                                           tggtgAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg      <  2:1090841/101‑1 (MQ=255)


                                                                                            ggtgAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     <  1:3905064/101‑1 (MQ=255)


                                                                                            ggtgAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     <  2:2755741/101‑1 (MQ=255)


                                                                                             gtgAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt    <  1:258658/101‑1 (MQ=255)


                                                                                             gtgAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt    >  1:1302367/1‑101 (MQ=255)


                                                                                               gAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  <  1:1919933/101‑1 (MQ=255)


                                                                                               gAACTCGGC.CCA.A.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  >  1:3881135/1‑101 (MQ=255)


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GC.CC..A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943

Base quality scores:


ATCG

< 3 ≤


ATCG

< 21 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG