Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,330 +G 100% intergenic (‑23/+32) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3301.→G100.0% 662.3 178intergenic (‑23/+32)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (80/96):  ref base (1/1):  total (81/97)

Only 100 of 207 total aligned reads displayed.

TTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.G.GGC..GG.GGTG..T.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGG  >  NC_014034/1974235‑1974418
                                                                                                  |                                                                                              
tttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.gg                                                                                          >  1:1068185/1‑101 (MQ=255)
tttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.gg                                                                                          >  2:2603393/1‑101 (MQ=255)
tttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.gg                                                                                          >  1:2687144/1‑101 (MQ=255)
  tCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.gggt                                                                                        <  1:2759397/101‑3 (MQ=255)
  tCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.gggg                                                                                        >  2:3290576/1‑99 (MQ=255)
   cACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.ggggg                                                                                       >  1:2871288/1‑98 (MQ=255)
    aCGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.gggtgt                                                                                      <  1:2647763/101‑5 (MQ=255)
     cGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.gggtgtc                                                                                     >  2:130245/1‑96 (MQ=255)
     cGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.gggtgtc                                                                                     <  1:1533107/101‑6 (MQ=255)
      ggCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGCCATGTCGGTCTCCTGAGGGGTTGG.GGC..gg.ggggggcc                                                                                    >  1:2891295/1‑95 (MQ=255)
         cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..T.c.cccc                                                                              >  1:1071448/1‑101 (MQ=255)
          gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..T.c.ccccc                                                                             <  1:1582168/101‑2 (MQ=255)
          gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..T.c.ccccc                                                                             <  1:1667946/101‑2 (MQ=255)
            aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..GGCGGGG..T.c.cccccgc                                                                           >  2:1879455/1‑98 (MQ=255)
              tCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..T.c.cccccgccc                                                                         <  1:2280292/101‑6 (MQ=255)
              tCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..T.c.cccccgccc                                                                         <  2:2214978/101‑6 (MQ=255)
               cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.Ccccgcccg                                                                         <  2:4124503/101‑1 (MQ=255)
                  cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGAt                                                                      <  2:1386036/101‑1 (MQ=255)
                  cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGGCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGAt                                                                      <  1:3767658/101‑1 (MQ=255)
                   gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATa                                                                     <  2:1625132/101‑1 (MQ=255)
                   gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGCTa                                                                     >  2:1595604/1‑101 (MQ=255)
                    aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGGTAg                                                                    >  2:2747796/1‑101 (MQ=255)
                    aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAg                                                                    <  1:3488973/101‑1 (MQ=255)
                     aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGGCATGTCGGTCTCCTGAGGGGTTGG.GGCGGGG.GGGG..C.C.CCCCGCCCGGATAGc                                                                   >  1:383091/1‑101 (MQ=255)
                     aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGGCGAAGGGCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGCTAGc                                                                   >  1:4103383/1‑101 (MQ=255)
                      gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCCCCCGGATAGCa                                                                  >  2:3999495/1‑101 (MQ=255)
                       tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGGTAggcc                                                                 >  1:1217359/1‑98 (MQ=255)
                        cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..GG.GGGGGCC.C.CCCCGCCCGGGTAGCAcg                                                                >  2:568007/1‑100 (MQ=255)
                         ggCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGCG..TCC.CCCCGCCCGGATAGCAcgg                                                               >  2:2977282/1‑99 (MQ=255)
                         ggCAGAAAGGATCGAGGGGGCGATCGTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCAcgg                                                               <  2:554353/101‑3 (MQ=255)
                          gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCAcggc                                                              <  1:2186767/101‑4 (MQ=255)
                          gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGGCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..T.CACCCCGCCCGGTTAggagggc                                                              >  1:1988573/1‑95 (MQ=255)
                           cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCAcggcg                                                             >  2:2649908/1‑97 (MQ=255)
                           cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGAtcggacggcg                                                             >  1:592353/1‑92 (MQ=255)
                           cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GAGGCG.GG.GGGG..TCC.CCCCGCCCGGATAGCAcggcg                                                             >  1:3825562/1‑97 (MQ=255)
                            aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGt                                                           <  1:505029/101‑1 (MQ=255)
                              aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTc                                                         <  1:3538213/101‑1 (MQ=255)
                               aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                        <  1:3628038/101‑1 (MQ=255)
                               aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                        >  2:3757809/1‑101 (MQ=255)
                                aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTTTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGGTAGCA.CGGCGTTCAg                                                       >  2:4229360/1‑101 (MQ=255)
                                   aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                    <  1:1191937/101‑2 (MQ=255)
                                   aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                    <  1:435154/101‑2 (MQ=255)
                                     cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                  <  2:1492998/101‑1 (MQ=255)
                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                 >  2:4329599/1‑101 (MQ=255)
                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                 <  1:2329881/101‑1 (MQ=255)
                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                 >  1:186470/1‑101 (MQ=255)
                                       aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGc                                                >  2:2996450/1‑101 (MQ=255)
                                         ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                              <  2:107899/101‑1 (MQ=255)
                                         ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                              >  2:826519/1‑101 (MQ=255)
                                         ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                              >  1:2299543/1‑101 (MQ=255)
                                          gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                             <  2:1070395/101‑1 (MQ=255)
                                          gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                             <  1:3269075/101‑1 (MQ=255)
                                           ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                            <  2:4234658/101‑1 (MQ=255)
                                           ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                            <  1:1423376/101‑1 (MQ=255)
                                           ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                            <  1:2876729/101‑1 (MQ=255)
                                            gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                           <  2:3613150/101‑1 (MQ=255)
                                            gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                           <  2:3446652/101‑1 (MQ=255)
                                              gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                         >  2:461755/1‑101 (MQ=255)
                                              gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                         <  2:1056586/101‑1 (MQ=255)
                                              gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGATAACA.CGGCGTTCAGCTCACGCCCAGAGc                                         >  2:3684817/1‑101 (MQ=255)
                                               aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                        >  1:2395322/1‑101 (MQ=255)
                                               aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                        <  1:1346614/101‑1 (MQ=255)
                                                 cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                      <  1:2034481/101‑1 (MQ=255)
                                                  ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                     <  1:2580123/101‑1 (MQ=255)
                                                   tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                    <  2:762413/101‑1 (MQ=255)
                                                      tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                 <  1:3204881/101‑1 (MQ=255)
                                                      tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                 >  1:3239161/1‑101 (MQ=255)
                                                        gAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                               >  1:1005816/1‑101 (MQ=255)
                                                          aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTGA.C.C.CCCCGCACGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                             <  2:2891087/101‑1 (MQ=255)
                                                          aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                             <  2:2195032/101‑1 (MQ=255)
                                                           cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                            <  2:3537514/101‑1 (MQ=255)
                                                            ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                           >  2:2180040/1‑101 (MQ=255)
                                                             gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                          <  1:130266/101‑1 (MQ=255)
                                                             gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                          >  1:3701899/1‑101 (MQ=255)
                                                               cGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                        >  2:627218/1‑101 (MQ=255)
                                                               cGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGATCACGCCCAGAGCGACGCGCCAGCCCGGAt                        <  1:3972908/101‑1 (MQ=255)
                                                                gAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                       <  2:2444367/101‑1 (MQ=255)
                                                                gAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                       >  2:1751063/1‑101 (MQ=255)
                                                                gAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                       <  2:4088036/101‑1 (MQ=255)
                                                                 aaGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                      <  2:385622/101‑1 (MQ=255)
                                                                  aGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCCGCCCGGATaga                     >  2:4383572/1‑101 (MQ=255)
                                                                   gggcATGTCGGTTTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                    <  1:4229621/98‑1 (MQ=255)
                                                                    gTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                   <  1:1473808/101‑1 (MQ=255)
                                                                      cATGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                 >  1:4096387/1‑101 (MQ=255)
                                                                       aTGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                <  2:612299/101‑1 (MQ=255)
                                                                        tGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt               >  2:2945887/1‑101 (MQ=255)
                                                                        tGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt               >  1:3411490/1‑101 (MQ=255)
                                                                        tGTCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGGG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt               >  1:966373/1‑101 (MQ=255)
                                                                          tCGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg             <  2:3361839/101‑1 (MQ=255)
                                                                           cGGTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCgg            <  1:389199/101‑1 (MQ=255)
                                                                             gTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCa          <  1:2671433/101‑1 (MQ=255)
                                                                             gTCTCCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCa          >  2:1545202/1‑101 (MQ=255)
                                                                              tctcCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt         <  2:2384203/101‑1 (MQ=255)
                                                                              tctcCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt         <  1:764722/101‑1 (MQ=255)
                                                                                tcCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg       <  1:2996671/101‑1 (MQ=255)
                                                                                tcCTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg       >  2:321683/1‑101 (MQ=255)
                                                                                 ccTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt      >  1:85883/1‑101 (MQ=255)
                                                                                 ccTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt      >  2:239431/1‑101 (MQ=255)
                                                                                  cTGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt     <  1:3037645/101‑1 (MQ=255)
                                                                                   tGAGGGGTTGG.GGCG.GG.GGTG..TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc    <  2:1197748/101‑1 (MQ=255)
                                                                                                  |                                                                                              
TTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.G.GGC..GG.GGTG..T.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGG  >  NC_014034/1974235‑1974418

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG