Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,337 +C 100% intergenic (‑30/+25) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3371.→C100.0% 735.5 197intergenic (‑30/+25)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (95/101):  ref base (1/0):  total (96/101)

Only 100 of 223 total aligned reads displayed.

GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.G.GG.C.G.G.GGTG.T.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433
                                                                                                        |                                                                                                
ggCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.T.cc                                                                                                <  1:2166725/101‑1 (MQ=255)
  ccGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.T.cccc                                                                                              <  2:614286/101‑1 (MQ=255)
   cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.T.ccccc                                                                                             >  1:2510484/1‑101 (MQ=255)
   cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.T.ccccc                                                                                             >  1:1071448/1‑101 (MQ=255)
    gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.T.cccccc                                                                                            <  1:1582168/101‑2 (MQ=255)
    gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.T.cccccc                                                                                            >  1:3077603/1‑100 (MQ=255)
      aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.C.G.GCGGGG.T.ccccccgc                                                                                          >  2:1879455/1‑98 (MQ=255)
       aTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.T.ccccccgcc                                                                                         >  2:4427759/1‑97 (MQ=255)
         cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCcccgcccg                                                                                        <  2:4124503/101‑1 (MQ=255)
            cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGAt                                                                                     <  2:1386036/101‑1 (MQ=255)
              aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAg                                                                                   <  1:3488973/101‑1 (MQ=255)
               aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGc                                                                                  <  1:3654079/101‑1 (MQ=255)
               aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGGCGAAGGGCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGCTAGc                                                                                  >  1:4103383/1‑101 (MQ=255)
                 tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGGTAggcc                                                                                >  1:1217359/1‑98 (MQ=255)
                   ggCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGATAGCAcgg                                                                              >  2:2209525/1‑99 (MQ=255)
                     cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCAcggcg                                                                            >  2:2649908/1‑97 (MQ=255)
                     cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGATAGCAcggcg                                                                            >  2:3972652/1‑97 (MQ=255)
                         aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCa                                                                       <  2:1254369/101‑1 (MQ=255)
                          aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTTTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGGTAGCA.CGGCGTTCAg                                                                      >  2:4229360/1‑101 (MQ=255)
                            gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                                    >  1:3711647/1‑101 (MQ=255)
                            gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCCCCCGGGTAGC.CCGGCGTTCAGtt                                                                    >  2:3825676/1‑101 (MQ=255)
                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                                   <  1:1191937/101‑2 (MQ=255)
                             aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                                   <  1:435154/101‑2 (MQ=255)
                              tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                                  <  1:2779758/101‑3 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                >  2:4329599/1‑101 (MQ=255)
                                 aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGc                                                               >  1:32278/1‑101 (MQ=255)
                                   ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                                             <  2:107899/101‑1 (MQ=255)
                                   ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                                             <  1:3576298/101‑1 (MQ=255)
                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            <  2:1070395/101‑1 (MQ=255)
                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            <  2:2994321/101‑1 (MQ=255)
                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            <  1:3269075/101‑1 (MQ=255)
                                     ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                           <  2:4234658/101‑1 (MQ=255)
                                     ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                           <  1:2876729/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          <  2:3446652/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          <  1:151826/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          <  1:499809/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          <  1:37849/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          >  1:1314065/1‑101 (MQ=255)
                                       cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                         >  2:1579383/1‑101 (MQ=255)
                                       cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                         <  2:722709/101‑1 (MQ=255)
                                        gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                        <  2:1056586/101‑1 (MQ=255)
                                        gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                        >  2:253361/1‑101 (MQ=255)
                                        gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGATAACA.CGGCGTTCAGCTCACGCCCAGAGc                                                        >  2:3684817/1‑101 (MQ=255)
                                            ttGATGGAACGGTCGAAGGTCATGTGGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTGTT.CCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                    <  2:569938/101‑1 (MQ=255)
                                            ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                    >  1:1704399/1‑101 (MQ=255)
                                             tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                                   <  2:1669491/101‑1 (MQ=255)
                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  >  1:2604437/1‑101 (MQ=255)
                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  >  1:2999234/1‑101 (MQ=255)
                                                 ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                               <  1:2921089/101‑1 (MQ=255)
                                                  gAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                              >  1:1005816/1‑101 (MQ=255)
                                                    aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTGAC.CCCCCGCACGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                                            <  2:2891087/101‑1 (MQ=255)
                                                     cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                           <  2:3537514/101‑1 (MQ=255)
                                                     cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                           >  2:1615769/1‑101 (MQ=255)
                                                      ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                                          >  2:2180040/1‑101 (MQ=255)
                                                      ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGGG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                                          >  1:3859631/1‑101 (MQ=255)
                                                       gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                         >  2:562655/1‑101 (MQ=255)
                                                       gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                         <  1:626914/101‑1 (MQ=255)
                                                       gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                         <  1:568059/101‑1 (MQ=255)
                                                        tCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGa                                        <  1:2304012/101‑1 (MQ=255)
                                                         cGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       <  2:1534566/101‑1 (MQ=255)
                                                         cGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGATCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       <  1:3972908/101‑1 (MQ=255)
                                                          gAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                      <  2:4088036/101‑1 (MQ=255)
                                                           aaGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                     <  2:385622/101‑1 (MQ=255)
                                                           aaGGTCATGTCGGTCTCCTGAGGGGTTGG.GG.C.GAC.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                     <  1:3825929/101‑1 (MQ=255)
                                                             gggcATGTCGGTTTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                                   <  1:4229621/98‑1 (MQ=255)
                                                              gTCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                                  <  1:2387016/101‑1 (MQ=255)
                                                               tCATGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCt                                 >  1:1902622/1‑101 (MQ=255)
                                                                 aTGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                               <  1:228205/101‑1 (MQ=255)
                                                                 aTGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                               >  2:1161641/1‑101 (MQ=255)
                                                                  tGTCGGTTTCCTGAGGGGGT.GCGGTT.G.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGGTCGCGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                              <  2:797986/101‑1 (MQ=255)
                                                                  tGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                              >  2:2945887/1‑101 (MQ=255)
                                                                  tGTCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                              >  1:3411490/1‑101 (MQ=255)
                                                                    tCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                            <  2:2395172/101‑1 (MQ=255)
                                                                    tCGGTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                            <  1:1994905/101‑1 (MQ=255)
                                                                       gTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCa                         <  1:2671433/101‑1 (MQ=255)
                                                                       gTCTCCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCa                         <  1:1432668/101‑1 (MQ=255)
                                                                        tctcCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        <  1:764722/101‑1 (MQ=255)
                                                                        tctcCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt                        <  2:2384203/101‑1 (MQ=255)
                                                                         ctcCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                       <  2:493096/101‑1 (MQ=255)
                                                                         ctcCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                       <  1:4051828/101‑1 (MQ=255)
                                                                         ctcCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCCTTCAGTTCACGCCCAGAGCGACGCCCCAGCCCGGATAGAGCTTGTCGGCATc                       >  2:1450148/1‑101 (MQ=255)
                                                                          tcCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                      >  2:321683/1‑101 (MQ=255)
                                                                          tcCTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                      <  1:1393532/101‑1 (MQ=255)
                                                                           ccTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt                     >  1:85883/1‑101 (MQ=255)
                                                                           ccTGAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt                     <  2:3057395/101‑1 (MQ=255)
                                                                              gAGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                  >  2:3310743/1‑101 (MQ=255)
                                                                               aGGGGTTGG.GG.CGG.G.GGTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                 >  2:2244351/1‑101 (MQ=255)
                                                                                    ggttggggcgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGaa              >  1:2044624/11‑101 (MQ=255)
                                                                                    ggttggggcgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGaa              <  2:2772632/91‑1 (MQ=255)
                                                                                    ggttggggcgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGaa              <  2:2718711/91‑1 (MQ=255)
                                                                                       tggggcgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  2:826977/8‑101 (MQ=255)
                                                                                       tggggcgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           <  2:32265/94‑1 (MQ=255)
                                                                                        ggggcgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          >  2:241507/7‑101 (MQ=255)
                                                                                         gggcgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCttt         >  2:3874288/6‑101 (MQ=255)
                                                                                         gggcgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCttt         <  2:1012582/96‑1 (MQ=255)
                                                                                            cgg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa      >  2:361598/3‑101 (MQ=255)
                                                                                             gg.g.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa     >  1:930175/2‑101 (MQ=255)
                                                                                             cgtg.ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa      >  1:3305862/4‑101 (MQ=255)
                                                                                                  ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGc  >  1:3892754/1‑101 (MQ=255)
                                                                                                  ggTG.TCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCCAACGc  >  1:2226886/1‑101 (MQ=255)
                                                                                                        |                                                                                                
GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.G.GG.C.G.G.GGTG.T.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG