Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,979,921 +G 2.9% intergenic (‑37/+47) tkt1 ← / ← cbbP transketolase/phosphoribulokinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,979,9211.→G 2.9% 7.9 ‑2.7 ‑0.0171intergenic (‑37/+47)tkt1/cbbPtransketolase/phosphoribulokinase
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (89/77):  total (94/77)
Fisher's exact test strand distribution p-value = 6.50e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.03e-01

Only 100 of 209 total aligned reads displayed.

CCATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979821‑1980021
                                                                                                      |                                                                                                       
  atcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGcgg.c.gg                                                                                                       >  2:2109192/1‑101 (MQ=255)
  atcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGcgg.c.gg                                                                                                       >  1:3920890/1‑101 (MQ=255)
  atcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATACTTCATGGCTCCTCCTCCATGATGAGCCTTTCGTCCGAGcgg.c.gg                                                                                                       >  1:3737631/1‑101 (MQ=255)
   tcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.ggg                                                                                                      <  1:971234/101‑1 (MQ=255)
    cGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.c                                                                                                    >  1:4410674/1‑101 (MQ=255)
     gCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.cc                                                                                                   >  2:3641995/1‑101 (MQ=255)
      ccAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.gcc                                                                                                  >  2:4254758/1‑101 (MQ=255)
        aGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCgg                                                                                                >  1:50906/1‑101 (MQ=255)
         gcgcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGt                                                                                               <  2:2310143/101‑1 (MQ=255)
          cgcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.CGGGG.CCCggg                                                                                               >  2:1546414/1‑100 (MQ=255)
           gcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTgg                                                                                             <  1:2232389/101‑1 (MQ=255)
            cccGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.ccccggggc                                                                                            >  2:3119401/1‑95 (MQ=255)
             ccGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTgggc                                                                                           <  1:3145765/101‑1 (MQ=255)
             ccGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTgggc                                                                                           <  1:3089989/101‑1 (MQ=255)
             ccGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGgggc                                                                                           >  1:3960002/1‑101 (MQ=255)
              cGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCCGGgggcc                                                                                          >  1:1933121/1‑101 (MQ=255)
              cGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTgggcc                                                                                          <  2:100650/101‑1 (MQ=255)
                gATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTgggccgg                                                                                        <  1:3606975/101‑1 (MQ=255)
                gATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGggggcgg                                                                                        >  1:295955/1‑101 (MQ=255)
                gATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGTggggggg                                                                                        >  1:777010/1‑97 (MQ=255)
                gATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGCgggcccg                                                                                        >  1:4272191/1‑99 (MQ=255)
                 aTGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCCGGggggcggg                                                                                       >  2:2274638/1‑101 (MQ=255)
                 aTGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTgggccggg                                                                                       <  1:4141422/101‑1 (MQ=255)
                 aTGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTgggccggg                                                                                       <  1:2160375/101‑1 (MQ=255)
                 aTGGCATTGGCCATCCGCGTTCCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCCCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTgggccggg                                                                                       <  1:3056376/101‑1 (MQ=255)
                  tGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCGGGgggccgggc                                                                                      >  2:3202706/1‑101 (MQ=255)
                   ggCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGggccccgggc                                                                                     >  2:624171/1‑99 (MQ=255)
                   ggCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGggggcgggcc                                                                                     >  1:1628622/1‑101 (MQ=255)
                     cATTGGCCATCCGCTTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTc                                                                                   <  1:2871047/101‑1 (MQ=255)
                        tcgCCATCCGCGTTTCCTGCGCCATATCGAGATCATTCATGGCTCCTTCTCCCTGATGAGCCTTTCGTCCGAGAGG.C.GGG.GCCAGTcggacggggcactcc                                                                                >  2:2997196/3‑94 (MQ=255)
                        tGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCGGGggggcggggcccccc                                                                                >  2:2620066/1‑94 (MQ=255)
                         ggCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCGGGGGGCCGGGGCCCGCCa                                                                               >  1:2519829/1‑101 (MQ=255)
                         ggCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGCCCGGCCTCGccc                                                                               >  1:3277519/1‑100 (MQ=255)
                          gCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGGCCCCGGGGGGCCGGCCCCCCCCa                                                                               >  1:1138427/1‑101 (MQ=255)
                           ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGCC.GGG.CCCGG.GGGCACGGCCTCGcccac                                                                             >  2:2413178/1‑98 (MQ=255)
                            cATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGGCGGGCCCCCCCCATg                                                                            >  1:2193177/1‑101 (MQ=255)
                            cATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATg                                                                            <  1:908315/101‑1 (MQ=255)
                              tCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGCCCGGGCCCGCCCATGTc                                                                          >  2:2008334/1‑101 (MQ=255)
                               ccGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCt                                                                         <  2:268498/101‑1 (MQ=255)
                                 gcgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCCGGGGGCCGGGGCTCGGCCAAGTCttt                                                                       >  2:4344967/1‑100 (MQ=255)
                                    tttCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGGCGGGGCCCGCCCATGTCTTGaaa                                                                    >  1:458035/1‑101 (MQ=255)
                                     ttCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGGCGGGGCCCGCCCATGTCTTGaaaa                                                                   >  2:473759/1‑100 (MQ=255)
                                      tCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCGCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATc                                                                  <  1:3813494/101‑1 (MQ=255)
                                      tCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATc                                                                  <  2:3465052/101‑1 (MQ=255)
                                      tCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATc                                                                  <  1:1166784/101‑1 (MQ=255)
                                      tCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGGCGGGGCCCGCCCATGTCTTTaaaac                                                                  >  1:3222413/1‑99 (MQ=255)
                                       ccTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGGACCCGGGGGGCCGGG.CTCGCCAATGTCCTGGAAttc                                                                 >  2:3567343/1‑99 (MQ=255)
                                           cgcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCGGGGGGGCGGGCCTCGCCAATGTCTTGAAAAC.GTTcc                                                            >  1:1363023/1‑101 (MQ=255)
                                             ccATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGTGCGGCGGGCCCCGCCAATGTCCTGAAAAC.GTTCCgg                                                          >  1:3826773/1‑101 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGGGGCCGGGCCCCGCCAAAGTCTTTAAAAC.GTTcccggt                                                        >  1:379260/1‑97 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGCCGGGCCCCGCCCATGTCTTGAAATC.GTTCCCGtt                                                        >  1:3407650/1‑101 (MQ=255)
                                                tatCGAGTGCCTTCATGGCTCCCCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTc                                                       <  2:3958423/101‑1 (MQ=255)
                                                 atCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGGCGGGCCCCCCCCAAGTCTTTTAAAC.GTTCCCGTTCa                                                      >  1:2344975/1‑101 (MQ=255)
                                                 atCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGCCGGGCCCCCCCCATGTCTTTGAAAC.GTTCCCGGTCa                                                      >  2:3372537/1‑101 (MQ=255)
                                                  tCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAg                                                     <  2:457995/101‑1 (MQ=255)
                                                  tCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAg                                                     <  1:892524/101‑1 (MQ=255)
                                                        tCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCCGGGGGCCCGGCCCCGCCCATGTCTTTAAAAC.GTTTCGGGTCAGGGCcgc                                               >  2:1338339/1‑101 (MQ=255)
                                                          cTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCcgcgc                                             <  1:3497203/101‑1 (MQ=255)
                                                              aTGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.CGGGG.CCCGGT.GGCCCGGCCTCGCCCATGTCTTGGAACCGGTCCCGGTTCA.GCCcccgcgcgg                                         >  2:697730/1‑100 (MQ=255)
                                                              aTGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCGGGGGGGCGGGGCTCGCCCATGGCCTTAAATC.GGTCCGGGTTCGGcccaggccgcg                                         >  2:853261/1‑93 (MQ=255)
                                                               tGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.c                                       <  2:1932992/101‑1 (MQ=255)
                                                                ggCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGGGGCCGGGCCTCGCCAACGTCTTGAAATA.GTTTCGGGTCAGGCCCGCGCGCGG.Cg                                      >  1:3379271/1‑101 (MQ=255)
                                                                 gCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.Cgg                                     <  1:2084709/101‑1 (MQ=255)
                                                                 gCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.Cgg                                     <  2:2471380/101‑1 (MQ=255)
                                                                  ctcctcctcCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.GCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAAAC.GTTCCGGGTCAGGCCCGCCCCCGG.CGGc                                    >  2:2739471/1‑101 (MQ=255)
                                                                     ctcctccCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCtt                                 <  2:1628516/101‑1 (MQ=255)
                                                                      tcctccCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTc                                <  1:1550201/101‑1 (MQ=255)
                                                                       cctccCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGCGGGGCCCGGCCTCGCCAATGTCTTTAAATC.GTTTCGGGTCAGGCCCCCGCGCGG.CGGccgccg                               >  1:840274/1‑97 (MQ=255)
                                                                        ctccCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTcgc                              <  2:50893/101‑1 (MQ=255)
                                                                         tccCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGGGGCCGGGGCTCGCCAATGTCTTGAAAAC.GTTCCGGGTTAGGGCCGCGCGGGACCGGCCtcccc                              >  1:2649162/1‑97 (MQ=255)
                                                                         tccCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGGGGCCGGGCCTCGCCAATGTCTTTAAATC.GTTCCGGTTCAGGCCCGCGCGCGG.CGGCCCTcgcg                             >  1:3230799/1‑101 (MQ=255)
                                                                          cccTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGGGGCCGGGGCTCGCCAATGTCTTTAAATC.GTTCCGGGTGAGGCCCCCGCGCGA.CGGGCTTcgccc                            >  2:4417998/1‑99 (MQ=255)
                                                                          cccTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGGGGGCCGGGCCTCGCCCATGTCTTTAAATC.GTTCCGGTTCAGGCCCGCGCCCGG.CCGCCCTcgccc                            >  1:2372284/1‑99 (MQ=255)
                                                                           ccTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCa                           <  1:2778890/101‑1 (MQ=255)
                                                                             tgatgaGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCCCGCCCATGTCTTTAAATC.GTTCCGGTTCCGGCCCCCGCCCGA.CGGCCTTTGCGCAcc                         >  1:2656789/1‑101 (MQ=255)
                                                                             tgatgaGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCCGGCCCCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGC.CGGGCCTCCCGCCcc                         >  2:3055520/1‑101 (MQ=255)
                                                                              gatgaGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCa                        <  2:2344825/101‑1 (MQ=255)
                                                                               atgaGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCCCGCGCGA.CGGCCTTCGCGCACCAg                       >  2:2872273/1‑101 (MQ=255)
                                                                                tgaGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGa                      <  1:473811/101‑1 (MQ=255)
                                                                                 gaGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGAc                     <  2:102378/101‑1 (MQ=255)
                                                                                 gaGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCCCGCCCATGGCTTGAAATT.GTTCCGGTTTACGGCCGCGCGCGA.CGGGCTTCGCGCACCCGAc                     >  1:1811421/1‑101 (MQ=255)
                                                                                   gCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGc                   <  2:3701500/101‑1 (MQ=255)
                                                                                   gCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGc                   <  1:1081669/101‑1 (MQ=255)
                                                                                   gCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGc                   <  2:2193038/101‑1 (MQ=255)
                                                                                     cTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTc                 <  2:4198774/101‑1 (MQ=255)
                                                                                      tttCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCg                <  1:1108962/101‑1 (MQ=255)
                                                                                          gTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCa            <  2:1986893/101‑1 (MQ=255)
                                                                                          gTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGAGCACCAGACGCTCGATCa            <  1:165097/101‑1 (MQ=255)
                                                                                          gTCCGAGCGG.C.GGG.CCCGGTGGGCCCGGCCTCGCCAATGGCTTGAAATC.CTTCCGGTTTAGGCCCGCCCGCCG.CGGGCTTCCCGCACCAGACGCTCCATcc            >  2:1778342/1‑100 (MQ=255)
                                                                                            ccGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCCATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGGTCAgc          >  2:78360/1‑101 (MQ=255)
                                                                                            ccGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCCGACCCTCGATCAgc          >  2:1104279/1‑101 (MQ=255)
                                                                                            ccGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAgc          <  1:624239/101‑1 (MQ=255)
                                                                                                  gg.c.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    <  2:3168088/101‑1 (MQ=255)
                                                                                                   g.c.ggG.CCGGGTGGCCGGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCa   <  2:3737378/101‑1 (MQ=255)
                                                                                                   g.c.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAGTC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCa   <  2:2975017/101‑1 (MQ=255)
                                                                                                   g.c.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCa   <  1:438877/101‑1 (MQ=255)
                                                                                                   g.c.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCa   >  1:2615880/1‑101 (MQ=255)
                                                                                                   g.c.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCa   >  1:2004295/1‑101 (MQ=255)
                                                                                                     c.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAg  <  2:890313/101‑1 (MQ=255)
                                                                                                     c.ggG.CCCGGTGGGCCGGGACTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAg  >  2:404163/1‑101 (MQ=255)
                                                                                                      |                                                                                                       
CCATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGG.C.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATC.GTTCCGGTTCAGGCCCGCGCGCGA.CGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979821‑1980021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG