Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,040,428 +C 98.3% intergenic (‑176/‑212) RCAP_rcc01888 ← / → RCAP_rcc01889 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,040,4281.→C98.3% 3.2 0.0 ‑1.8233intergenic (‑176/‑212)RCAP_rcc01888/RCAP_rcc01889hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (97/132):  ref base (2/2):  total (99/134)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.47e-02

Only 100 of 251 total aligned reads displayed.

GCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GG.C.GCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGCA  >  NC_014034/2040326‑2040525
                                                                                                        |                                                                                                  
gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.gg                                                                                                     <  2:3674169/101‑1 (MQ=255)
gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.gg                                                                                                     >  1:2664289/1‑101 (MQ=255)
       gtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.Gg.ccgcgc                                                                                              <  1:981880/99‑6 (MQ=255)
        tgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGcc                                                                                             <  1:585279/100‑1 (MQ=255)
        tgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGcc                                                                                             >  1:313651/2‑101 (MQ=255)
         gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCa                                                                                            <  2:1027382/101‑1 (MQ=255)
         gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCa                                                                                            <  1:4314540/101‑1 (MQ=255)
         gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCa                                                                                            <  1:3031752/101‑1 (MQ=255)
           gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATa                                                                                          >  2:4196161/1‑101 (MQ=255)
           gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATa                                                                                          <  2:1308429/101‑1 (MQ=255)
           gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATa                                                                                          <  2:244809/101‑1 (MQ=255)
              aTCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAA.GGCC.GCGGCATAAGt                                                                                       >  2:1333716/1‑101 (MQ=255)
               gccGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTg                                                                                      <  2:2815790/100‑1 (MQ=255)
               gccGGTAGAAGGTCAGACGTTGTGCAAAAGC.CCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCTGCCGCAAA.GGCC.GCGCCATAAGTg                                                                                      <  2:2149204/100‑1 (MQ=255)
                ccGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGa                                                                                     <  2:2125946/101‑1 (MQ=255)
                 cGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAg                                                                                    <  2:3561154/101‑1 (MQ=255)
                 cGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAA.GGAC.GCGCCATAAGTGAg                                                                                    >  2:1532962/1‑101 (MQ=255)
                  ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGc                                                                                   <  2:2696558/101‑1 (MQ=255)
                  ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGc                                                                                   >  2:2629649/1‑101 (MQ=255)
                   gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGcc                                                                                  >  2:2351103/1‑101 (MQ=255)
                     cGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCa                                                                                <  2:3240908/101‑1 (MQ=255)
                       aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAcc                                                                              <  2:2597847/101‑1 (MQ=255)
                       aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAcc                                                                              <  1:1663442/101‑1 (MQ=255)
                       aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAcc                                                                              <  1:2778557/101‑1 (MQ=255)
                        aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAccg                                                                             <  1:2304063/101‑2 (MQ=255)
                        aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAccg                                                                             >  2:56415/1‑100 (MQ=255)
                         ggTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAccgg                                                                            >  2:1628025/1‑99 (MQ=255)
                         ggTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAccgg                                                                            >  2:1374663/1‑99 (MQ=255)
                          gTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAccggt                                                                           <  1:3880398/101‑4 (MQ=255)
                          gTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAA.GGCC.GCGCCATAAGTTAGCCCAccggt                                                                           >  2:474173/1‑98 (MQ=255)
                           tCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAccggtt                                                                          <  2:2263298/101‑5 (MQ=255)
                           tCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCAccggtt                                                                          >  2:1759007/1‑97 (MQ=255)
                             aGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCg                                                                       >  2:3144760/1‑101 (MQ=255)
                                 gTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAgg                                                                   >  1:3284988/1‑101 (MQ=255)
                                   tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt                                                                 <  1:3783343/101‑3 (MQ=255)
                                     tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg                                                               <  1:3495859/101‑5 (MQ=255)
                                     tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg                                                               <  2:4238664/101‑5 (MQ=255)
                                     tgCAAAAGCA.CCGGGGCGGATGGCTTAAGCGGTATGGCGCGTGCCAAAGACGTAGGCCGCAAACGC.C.GCGCCATAAGTGAGCCCA.CTGGTTCGGAggttgg                                                               >  2:2978642/1‑97 (MQ=255)
                                      gCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGa                                                             <  1:2393960/101‑1 (MQ=255)
                                      gCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGa                                                             >  2:767863/1‑101 (MQ=255)
                                       cAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAGGGtggat                                                             >  1:3157899/1‑97 (MQ=255)
                                        aaaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATg                                                           <  1:1776051/101‑1 (MQ=255)
                                         aaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGc                                                          >  1:2692113/1‑101 (MQ=255)
                                             gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa                                                       >  1:2258681/4‑101 (MQ=255)
                                               accGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaaa                                                     >  1:2386149/2‑101 (MQ=255)
                                                ccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAAt                                                    <  1:4184034/101‑1 (MQ=255)
                                                ccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAAt                                                    <  1:1413900/101‑1 (MQ=255)
                                                ccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAAt                                                    <  1:152365/101‑1 (MQ=255)
                                                 cGGGTCTGATGGCTTACGCGGTATGGCGCGTGCCACAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATc                                                   <  2:3004593/101‑1 (MQ=255)
                                                  ggggCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCg                                                  >  2:946594/1‑101 (MQ=255)
                                                  ggggCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCg                                                  >  1:194937/1‑101 (MQ=255)
                                                      cGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCAt                                              <  1:2069680/101‑1 (MQ=255)
                                                       ggATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATg                                             <  1:187580/101‑1 (MQ=255)
                                                       ggATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATg                                             >  1:51859/1‑101 (MQ=255)
                                                         gtGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGAt                                           <  1:4118671/100‑1 (MQ=255)
                                                         aTGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGAt                                           <  1:650235/101‑1 (MQ=255)
                                                           ggCTTACGCGGTATGGCGCGTGCCAACGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATcc                                         <  1:3736336/101‑1 (MQ=255)
                                                           ggCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATcc                                         >  2:508055/1‑101 (MQ=255)
                                                            gCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCg                                        <  1:2836783/101‑1 (MQ=255)
                                                             cTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGc                                       <  2:1805169/101‑1 (MQ=255)
                                                               tACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGccc                                     <  2:856842/101‑1 (MQ=255)
                                                               tACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGccc                                     <  2:25175/101‑1 (MQ=255)
                                                                aCGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGccct                                    <  2:403891/101‑1 (MQ=255)
                                                                 cgcgGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGccctc                                   <  2:1971002/101‑1 (MQ=255)
                                                                 cgcgGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGccctc                                   <  2:2296936/101‑1 (MQ=255)
                                                                     gTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGATGGATGCATAAATCGTCATGATCCGccctccctc                               >  2:1297734/1‑100 (MQ=255)
                                                                      tATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGt                              <  1:2625730/101‑1 (MQ=255)
                                                                        tGGCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCt                            <  1:2821552/101‑1 (MQ=255)
                                                                         ggCGCGTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTg                           <  1:1311481/101‑1 (MQ=255)
                                                                           cgcgTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGaa                         <  1:4065430/101‑1 (MQ=255)
                                                                            gcgTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAAt                        <  2:3451016/101‑1 (MQ=255)
                                                                            gcgTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCGGAAt                        <  2:3766917/101‑1 (MQ=255)
                                                                             cgTGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAAtt                       <  1:4196422/101‑1 (MQ=255)
                                                                               tGCCAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGc                     <  1:1250437/101‑1 (MQ=255)
                                                                                 ccAAAGACGTCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCg                   <  2:3903390/101‑1 (MQ=255)
                                                                                  cAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCgg                  <  1:2351253/101‑1 (MQ=255)
                                                                                  cAAAGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCgg                  <  1:4221628/101‑1 (MQ=255)
                                                                                   aaaGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGa                 >  1:1015700/1‑101 (MQ=255)
                                                                                   aaaGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGa                 >  2:1713826/1‑101 (MQ=255)
                                                                                    aaGACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGAt                >  2:2358127/1‑101 (MQ=255)
                                                                                      gACGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGc              <  2:1846027/101‑1 (MQ=255)
                                                                                       aCGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCg             <  1:1038784/101‑1 (MQ=255)
                                                                                       aCGGCGGCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCg             <  2:3716115/101‑1 (MQ=255)
                                                                                        cggcggCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCgg            <  1:1158795/101‑1 (MQ=255)
                                                                                        cggcggCCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCgg            <  1:1781339/101‑1 (MQ=255)
                                                                                         ggcggcCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCggg           >  2:1133546/1‑101 (MQ=255)
                                                                                         ggcggcCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCggg           >  2:3370606/1‑101 (MQ=255)
                                                                                            ggcCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCaa        >  1:4305417/1‑101 (MQ=255)
                                                                                            ggcCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCaa        <  1:4268077/101‑1 (MQ=255)
                                                                                            ggcCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCaa        >  1:925413/1‑101 (MQ=255)
                                                                                             gcCGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCaaa       >  1:3919796/1‑101 (MQ=255)
                                                                                               cGCAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAAt     >  2:584828/1‑101 (MQ=255)
                                                                                               cGCAAA.GG.CTGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAAt     <  1:827146/101‑1 (MQ=255)
                                                                                                 cAAA.GGCC.GCGCGATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGc   >  1:3192339/1‑101 (MQ=255)
                                                                                                 cAAA.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGc   <  2:4279586/101‑1 (MQ=255)
                                                                                                  aaa.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGCa  >  2:3882703/1‑101 (MQ=255)
                                                                                                  aaa.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGCa  >  2:3995725/1‑101 (MQ=255)
                                                                                                  aaa.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGCa  >  1:476832/1‑101 (MQ=255)
                                                                                                  aaa.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGCa  <  2:4141195/101‑1 (MQ=255)
                                                                                                  aaa.GGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGCa  <  2:1978750/101‑1 (MQ=255)
                                                                                                        |                                                                                                  
GCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAA.GG.C.GCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATCGTCATGATCCGCCCTCCCTGTCTGAATTGCCGGATGCGGGCAAAATGCA  >  NC_014034/2040326‑2040525

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG