Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,047,090 +C 93.3% coding (20/879 nt) potH1 ← polyamine ABC transporter permease PotH

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,047,0901.→C93.3% 33.8 ‑0.3 ‑1.6195coding (20/879 nt)potH1polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand):  new base (84/98):  ref base (5/8):  total (89/106)
Fisher's exact test strand distribution p-value = 7.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.49e-02

Only 100 of 203 total aligned reads displayed.

CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CG.GG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180
                                                                                                    |                                                                                               
cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.c                                                                                                <  1:3235785/101‑1 (MQ=255)
  acgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.ccg                                                                                              <  2:1553203/101‑3 (MQ=255)
   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Cc.gg                                                                                            <  2:1113771/101‑1 (MQ=255)
     agggTCACCAGCGGGGCCGCCAGCAACAGAACGGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Cc.gggt                                                                                          <  1:918900/99‑3 (MQ=255)
      tGGTCCCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Cc.gggtt                                                                                         <  1:592333/101‑4 (MQ=255)
      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Cc.gggtt                                                                                         >  1:2120715/1‑98 (MQ=255)
        gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Cc.gggttgg                                                                                       >  2:3109227/1‑96 (MQ=255)
         tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Cc.gggttgga                                                                                      >  2:1238206/1‑95 (MQ=255)
         tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Cc.gggttgga                                                                                      <  2:526165/101‑7 (MQ=255)
           aCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Cc.gggttggaga                                                                                    >  2:2132603/1‑93 (MQ=255)
            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGAt                                                                                    >  2:4283438/1‑101 (MQ=255)
            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGAt                                                                                    <  1:3575397/101‑1 (MQ=255)
               gCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGcc                                                                                 >  2:3816154/1‑101 (MQ=255)
               gCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGcc                                                                                 <  2:40049/101‑1 (MQ=255)
               gCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGcc                                                                                 <  2:4048207/101‑1 (MQ=255)
               gCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGcc                                                                                 <  2:563316/101‑1 (MQ=255)
                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCg                                                                                >  1:3081979/1‑101 (MQ=255)
                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGa                                                                               <  2:1281671/101‑1 (MQ=255)
                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGa                                                                               >  1:482947/1‑101 (MQ=255)
                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGAc                                                                              >  1:1628580/1‑101 (MQ=255)
                    gccgccAGCAACAGAACTGCATAGATCGGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACAt                                                                            <  1:438728/101‑1 (MQ=255)
                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATAct                                                                         <  2:1652303/101‑1 (MQ=255)
                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATAct                                                                         <  2:2358908/101‑1 (MQ=255)
                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATActc                                                                        <  2:4390767/101‑1 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATActct                                                                       <  1:131683/101‑1 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATActct                                                                       >  2:879281/1‑101 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATActct                                                                       >  1:2797358/1‑101 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATActct                                                                       <  2:685573/101‑1 (MQ=255)
                          aGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTa                                                                      >  1:215851/1‑101 (MQ=255)
                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTAt                                                                     <  1:253573/101‑1 (MQ=255)
                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTAtt                                                                    >  2:637150/1‑101 (MQ=255)
                             aaCAGAACTGCATAGATCAGCGTTGGGCTGCCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATtc                                                                   <  2:2252626/101‑1 (MQ=255)
                             aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATtc                                                                   >  2:749850/1‑101 (MQ=255)
                             aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAAATGCCGACATACTCTATtc                                                                   <  1:2084724/101‑1 (MQ=255)
                              aCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATtct                                                                  >  2:3585369/1‑101 (MQ=255)
                              aCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATtct                                                                  >  2:1818727/1‑101 (MQ=255)
                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTct.cc                                                               >  1:816154/1‑101 (MQ=255)
                                  aaCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTct.ccct                                                             <  2:2559670/101‑3 (MQ=255)
                                   acagCACAGATGCGCGTTGGGCTGGCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCCCCGCCG.GGTTGGAGATGCCGACATACTCTATTct.ccctc                                                            <  1:1256818/98‑4 (MQ=255)
                                   aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTct.ccctc                                                            <  1:3868039/101‑4 (MQ=255)
                                    cTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTct.ccctca                                                           >  1:1745386/1‑97 (MQ=255)
                                      gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAgc                                                          >  1:2787497/1‑101 (MQ=255)
                                       cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcg                                                         >  1:668390/1‑101 (MQ=255)
                                       cATAAATCAGCGTTGGGCTGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcg                                                         <  1:51911/101‑1 (MQ=255)
                                           gaccAGGGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtc                                                     <  1:1136111/98‑4 (MQ=255)
                                           gATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtc                                                     >  2:32171/1‑98 (MQ=255)
                                             tCAGCTTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtctc                                                   <  1:3719845/101‑6 (MQ=255)
                                             tCAGCGTTGGGCTGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtctc                                                   <  2:3062822/101‑6 (MQ=255)
                                              cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCa                                                  <  1:3063130/101‑1 (MQ=255)
                                               aGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTTTTCTCCCTCAGCGCGTCTCA.g                                                <  1:3297179/101‑1 (MQ=255)
                                                 ggTTGGGCGGACCAAGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggc                                              <  1:2678322/100‑3 (MQ=255)
                                                 cGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggc                                              >  1:538608/1‑99 (MQ=255)
                                                 cGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggc                                              >  2:4204872/1‑99 (MQ=255)
                                                  gTTGGGTTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGCAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcg                                             <  2:2277299/101‑4 (MQ=255)
                                                  gTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcg                                             <  2:1401825/101‑4 (MQ=255)
                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                           >  1:3664924/1‑96 (MQ=255)
                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                           >  1:1878106/1‑96 (MQ=255)
                                                       gCTGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:2494168/101‑1 (MQ=255)
                                                          gACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTggg                                      <  1:4080627/101‑1 (MQ=255)
                                                          gACAATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTggg                                      <  1:879366/101‑1 (MQ=255)
                                                           aCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGc                                     <  1:1981961/101‑1 (MQ=255)
                                                           aCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGc                                     >  1:469751/1‑101 (MQ=255)
                                                            ccATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGAGT.TGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    <  1:417941/101‑1 (MQ=255)
                                                            ccATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    <  1:1483000/101‑1 (MQ=255)
                                                             cATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCa                                   >  1:4300710/1‑101 (MQ=255)
                                                             cATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCa                                   >  1:2608576/1‑101 (MQ=255)
                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  2:1173808/1‑101 (MQ=255)
                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  2:3346606/1‑101 (MQ=255)
                                                               tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaaa                                 >  1:737615/1‑101 (MQ=255)
                                                                gacccAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                <  1:1417810/98‑1 (MQ=255)
                                                                   ccAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTg                             <  2:2774166/101‑1 (MQ=255)
                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                           >  1:1139952/1‑101 (MQ=255)
                                                                       ccGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGcaca                         <  2:1570921/101‑1 (MQ=255)
                                                                        cGCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACaa                        <  1:2670277/101‑1 (MQ=255)
                                                                         gCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       >  2:1021760/1‑101 (MQ=255)
                                                                         gCTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       <  2:2664100/101‑1 (MQ=255)
                                                                          cTGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAgg                      >  1:2272582/1‑101 (MQ=255)
                                                                           tGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     >  2:1867500/1‑101 (MQ=255)
                                                                           tGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     >  1:568969/1‑101 (MQ=255)
                                                                           tGGCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     >  1:1803305/1‑101 (MQ=255)
                                                                            ggCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    <  1:604617/101‑1 (MQ=255)
                                                                            ggCCGCCTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    <  1:3960649/101‑1 (MQ=255)
                                                                              ccgccTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  <  2:2868936/101‑1 (MQ=255)
                                                                                gccTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                <  2:3943890/101‑1 (MQ=255)
                                                                                gccTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                >  2:927795/1‑101 (MQ=255)
                                                                                gccTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                >  2:2679998/1‑101 (MQ=255)
                                                                                gccTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                <  2:2016308/101‑1 (MQ=255)
                                                                                gccTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                <  1:3691406/101‑1 (MQ=255)
                                                                                  cTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa              <  2:469699/101‑1 (MQ=255)
                                                                                  cTCCGAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa              <  1:1369849/101‑1 (MQ=255)
                                                                                      gAGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg          >  1:4045826/1‑101 (MQ=255)
                                                                                       aGCGCGCCCACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGa         >  2:592980/1‑101 (MQ=255)
                                                                                         cgcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  2:3902538/96‑1 (MQ=255)
                                                                                         cgcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:709924/96‑1 (MQ=255)
                                                                                         cgcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:508105/96‑1 (MQ=255)
                                                                                          gcgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAg      >  1:2887631/5‑101 (MQ=255)
                                                                                           cgcccACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgg     >  2:3187383/4‑101 (MQ=255)
                                                                                              ccACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  2:2705669/101‑1 (MQ=255)
                                                                                              ccACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:1325670/101‑1 (MQ=255)
                                                                                              ccACCGCCG.GGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:1024268/101‑1 (MQ=255)
                                                                                                    |                                                                                               
CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CG.GG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG