Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,825 +C 76.7% intergenic (‑591/‑311) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8251.→C76.7% 116.2 ‑0.8 ‑0.2146intergenic (‑591/‑311)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (60/52):  ref base (21/13):  total (81/65)
Fisher's exact test strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.98e-01

Only 100 of 171 total aligned reads displayed.

AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057725‑2057913
                                                                                                     |                                                                                            
aaagattGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                               >  2:3769791/6‑101 (MQ=255)
 aaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c                                                                                             >  1:824164/1‑101 (MQ=255)
  aaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cc                                                                                            <  1:1195911/101‑1 (MQ=255)
   aTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.ccc                                                                                           <  2:3224685/101‑2 (MQ=255)
    tttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccg                                                                                          <  2:2706617/101‑3 (MQ=255)
    tttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccg                                                                                          >  2:287431/1‑99 (MQ=255)
     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccgc                                                                                         <  1:1193734/101‑4 (MQ=255)
     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccgc                                                                                         <  1:2048587/101‑4 (MQ=255)
      tGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccgcg                                                                                        >  1:3311997/1‑97 (MQ=255)
      tGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccgcg                                                                                        >  2:4417672/1‑97 (MQ=255)
       gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgc                                                                                     >  2:3062531/1‑101 (MQ=255)
       gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgc                                                                                     <  2:1728840/101‑1 (MQ=255)
        tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgcc                                                                                    >  2:358152/1‑100 (MQ=255)
        tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgcc                                                                                    >  2:4121195/1‑100 (MQ=255)
         ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgccg                                                                                   >  1:172037/1‑99 (MQ=255)
         ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgccg                                                                                   >  1:418313/1‑99 (MQ=255)
         ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgccg                                                                                   >  1:4229868/1‑99 (MQ=255)
          cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGc                                                                                 >  2:3675111/1‑101 (MQ=255)
            gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcag                                                                               >  2:1437077/1‑99 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACCAT.CC.CG.CGC.CGcaag                                                                              >  1:129673/1‑98 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaag                                                                              <  1:3015557/101‑4 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaag                                                                              <  1:2775956/101‑4 (MQ=255)
              ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                             <  2:1724051/101‑5 (MQ=255)
              ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                             <  1:1898453/101‑5 (MQ=255)
               tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcg                                                                            >  1:4435652/1‑96 (MQ=255)
                 ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtt                                                                          <  1:3630583/101‑8 (MQ=255)
                  cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                         >  1:2695265/1‑93 (MQ=255)
                  cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                         >  1:369408/1‑93 (MQ=255)
                   gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                        <  1:836971/101‑10 (MQ=255)
                   gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                        <  1:4265518/101‑10 (MQ=255)
                   gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                        >  2:2014778/1‑92 (MQ=255)
                          aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCg                                                                     >  2:1645822/1‑101 (MQ=255)
                           aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCgg                                                                    <  1:668919/101‑1 (MQ=255)
                           aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCgg                                                                    >  1:4416326/1‑101 (MQ=255)
                           aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGCGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCgg                                                                    <  1:3111122/101‑1 (MQ=255)
                            cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                   >  1:1022663/1‑101 (MQ=255)
                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:4103195/101‑1 (MQ=255)
                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  1:1761062/101‑1 (MQ=255)
                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:1362119/101‑1 (MQ=255)
                               tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCacc                                                                >  2:945897/1‑99 (MQ=255)
                               tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATc                                                                >  1:1562326/1‑101 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                               <  1:2922857/101‑1 (MQ=255)
                                   tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTc                                                            <  2:821379/101‑1 (MQ=255)
                                   tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTc                                                            >  1:2235624/1‑101 (MQ=255)
                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                           >  2:1397781/1‑101 (MQ=255)
                                     ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                          >  1:2388367/1‑101 (MQ=255)
                                     ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                          <  1:897343/101‑1 (MQ=255)
                                     ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                          <  1:2810517/101‑1 (MQ=255)
                                      gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTg                                                         >  2:3223001/1‑101 (MQ=255)
                                      gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTg                                                         <  2:2628535/101‑1 (MQ=255)
                                        gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.c                                                      <  1:3171291/101‑1 (MQ=255)
                                         cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cc                                                     <  2:3311765/101‑1 (MQ=255)
                                         cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cc                                                     >  2:1542100/1‑101 (MQ=255)
                                         cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cc                                                     <  1:2492290/101‑1 (MQ=255)
                                          gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccc                                                    <  2:2496259/101‑1 (MQ=255)
                                           cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccc                                                   <  1:3369282/101‑1 (MQ=255)
                                            ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  1:1095787/101‑1 (MQ=255)
                                             gccTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  2:3536416/99‑2 (MQ=255)
                                             gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTgagatcgg                                                  >  1:3594459/1‑94 (MQ=255)
                                             gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 >  2:3401969/1‑100 (MQ=255)
                                             gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  1:237614/101‑2 (MQ=255)
                                              acgtCGGGGCGGCGTTGGGCCGCTTGCGATCCTGCGTTTCCGGTTTGTCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGT.ccccccg                                                <  2:2212249/98‑3 (MQ=255)
                                              aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                >  2:1498884/1‑99 (MQ=255)
                                              aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                <  1:3041996/101‑3 (MQ=255)
                                                ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              <  2:867080/101‑5 (MQ=255)
                                                ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  1:3102527/1‑97 (MQ=255)
                                                   ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                            >  1:2962680/1‑101 (MQ=255)
                                                     gccggcggcggGGCTCTTTCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGGGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          <  1:1787523/99‑1 (MQ=255)
                                                        ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       >  1:3487144/1‑101 (MQ=255)
                                                        ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       >  2:1009214/1‑101 (MQ=255)
                                                         gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATc                                      <  1:1045744/101‑1 (MQ=255)
                                                          cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     >  2:396294/1‑101 (MQ=255)
                                                           ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  2:729349/1‑101 (MQ=255)
                                                           ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:160037/101‑1 (MQ=255)
                                                             cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  >  2:2419610/1‑101 (MQ=255)
                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGCACCGGCATCATTCTTGGCCCCCCCCCGGGACAATCACGgc                                 >  1:1506127/1‑101 (MQ=255)
                                                                 gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgc                              <  2:316822/101‑1 (MQ=255)
                                                                   tctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:3208340/1‑101 (MQ=255)
                                                                   tctTGCGATCCTCCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  1:355174/1‑101 (MQ=255)
                                                                      tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  1:3340771/101‑1 (MQ=255)
                                                                      tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  1:1989796/1‑101 (MQ=255)
                                                                        cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  1:1434066/101‑1 (MQ=255)
                                                                        cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  1:3223230/101‑1 (MQ=255)
                                                                        cGATCCTGCGTTGCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCTCCTCATTg                       <  2:2668656/101‑1 (MQ=255)
                                                                         gATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  2:4060766/1‑101 (MQ=255)
                                                                           tCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:535928/1‑101 (MQ=255)
                                                                           tCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  2:1593571/1‑101 (MQ=255)
                                                                           tCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    <  1:3449967/101‑1 (MQ=255)
                                                                               gCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  1:4227015/1‑101 (MQ=255)
                                                                                cGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  2:2388217/101‑1 (MQ=255)
                                                                                cGTTTCCGGATTGCCAACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  2:1137425/101‑1 (MQ=255)
                                                                                  tttCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  2:4247806/1‑101 (MQ=255)
                                                                                  tttCCGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  2:1085681/101‑1 (MQ=255)
                                                                                     ccGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          >  2:1223077/1‑101 (MQ=255)
                                                                                      cGGATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGCCAATCCCGGCCGCCTCATTGTTCCGCCATTGCGa         >  1:2136476/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        <  2:1227143/101‑1 (MQ=255)
                                                                                          ttGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:4121455/101‑1 (MQ=255)
                                                                                           tGGCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  1:3604498/1‑101 (MQ=255)
                                                                                            ggCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCCTTGTTCCGCCATTGCGATCAAAg   >  1:411341/1‑101 (MQ=255)
                                                                                             gCGACAATCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  >  2:109513/1‑101 (MQ=255)
                                                                                                     |                                                                                            
AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057725‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG