Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,270 +AG 100% coding (107/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2701.→A100.0% 788.6 201coding (107/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (99/100):  ref base (0/1):  total (100/101)
*NC_0140342,177,2702.→G100.0% 764.8 201coding (107/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (99/100):  ref base (1/1):  total (100/101)

Only 100 of 221 total aligned reads displayed.

TGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCC.A.G.GCAGAGGCCCC  >  NC_014034/2177171‑2177354
                                                                                                     ||                                                                                             
tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaaa                                                                                                <  2:651701/101‑2 (MQ=255)
tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaaa                                                                                                <  1:2139901/101‑2 (MQ=255)
tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaaa                                                                                                <  1:1884405/101‑2 (MQ=255)
 gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaaag                                                                                               >  2:3392993/1‑99 (MQ=255)
 gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaaag                                                                                               >  1:2741222/1‑99 (MQ=255)
   gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..aggg                                                                                           <  2:4039782/101‑2 (MQ=255)
   gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGACTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..aggg                                                                                           >  2:317461/1‑100 (MQ=255)
     ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..aggggt                                                                                         >  2:395446/1‑98 (MQ=255)
       ccACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..aggggttg                                                                                       <  1:505994/101‑6 (MQ=255)
        cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..aggggttgg                                                                                      <  2:1217170/101‑7 (MQ=255)
          caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTg..gtg                                                                                    <  1:1722885/101‑2 (MQ=255)
           aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGGCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTg..gtgt                                                                                   <  1:3530811/101‑3 (MQ=255)
            tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTg..gtgtc                                                                                  <  2:2248382/101‑4 (MQ=255)
            tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTg..gtgtc                                                                                  >  1:906535/1‑98 (MQ=255)
                  tCGATCTTGCCCGCATAGGCCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTg..gtgtcggtctt                                                                            <  2:452971/101‑10 (MQ=255)
                    gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.gg                                                                         >  1:3008210/1‑100 (MQ=255)
                    gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.gg                                                                         <  2:2487514/101‑2 (MQ=255)
                     aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggt                                                                        >  2:4344673/1‑99 (MQ=255)
                     aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggt                                                                        <  1:1878516/101‑3 (MQ=255)
                     aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggt                                                                        <  2:230209/101‑3 (MQ=255)
                     aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggt                                                                        <  2:3062887/101‑3 (MQ=255)
                      tCTTGCCCGCATAGGTCGAGAGCACCAGGCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtg                                                                       >  1:733218/1‑98 (MQ=255)
                      tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtg                                                                       <  2:2105296/101‑4 (MQ=255)
                         tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtgatc                                                                    >  2:1464102/1‑95 (MQ=255)
                          gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtgatca                                                                   >  2:4334528/1‑94 (MQ=255)
                          gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtgatca                                                                   <  1:1409792/101‑8 (MQ=255)
                           cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcag                                                                  <  1:1043377/101‑9 (MQ=255)
                           cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcag                                                                  <  1:1687164/101‑9 (MQ=255)
                            ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcaga                                                                 <  1:3185832/101‑10 (MQ=255)
                            ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcaga                                                                 <  2:2519860/101‑10 (MQ=255)
                             cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTt.ggtgatcagac                                                                <  1:2172398/101‑11 (MQ=255)
                               cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGAtcagacag                                                               >  1:2868463/1‑101 (MQ=255)
                               cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGAtcagacag                                                               >  2:829782/1‑101 (MQ=255)
                               cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTGTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGAtcagacag                                                               <  1:3758648/101‑1 (MQ=255)
                                aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAgg                                                              >  1:106419/1‑101 (MQ=255)
                                  aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGcc                                                            >  1:4059637/1‑101 (MQ=255)
                                    gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGcccc                                                          <  1:2891685/101‑1 (MQ=255)
                                     tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCg                                                         <  1:870670/101‑1 (MQ=255)
                                       gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                       <  1:1106733/101‑1 (MQ=255)
                                        agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGtt                                                      >  1:4425319/4‑101 (MQ=255)
                                         gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                     <  2:2357175/99‑1 (MQ=255)
                                           gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                   <  2:3880672/101‑1 (MQ=255)
                                            cACGAGCGCCATTTCGGTCGCTTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTG..GTGGCGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                  <  2:3701899/101‑1 (MQ=255)
                                             cccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                                 <  2:10533/100‑1 (MQ=255)
                                             aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                                 >  1:4219046/1‑101 (MQ=255)
                                             aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                                 <  2:2988381/101‑1 (MQ=255)
                                               cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                               <  2:2716622/101‑1 (MQ=255)
                                               cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                               <  2:2614858/101‑1 (MQ=255)
                                               cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                               >  2:3585122/1‑101 (MQ=255)
                                                aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                             <  2:2895388/101‑1 (MQ=255)
                                                 gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                            <  1:940063/101‑2 (MQ=255)
                                                  ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aag                                           <  1:236947/101‑3 (MQ=255)
                                                    ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                         >  2:413690/1‑97 (MQ=255)
                                                     cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagccg                                        >  2:1112506/1‑96 (MQ=255)
                                                      aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgc                                        >  2:163918/1‑101 (MQ=255)
                                                       tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                       >  2:784204/1‑101 (MQ=255)
                                                         tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGTCCCCGGTTTCGGCCGAAGCCGcccc                                     >  2:1268592/1‑101 (MQ=255)
                                                         tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                     <  1:2260084/101‑1 (MQ=255)
                                                             tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                 >  2:3282516/1‑101 (MQ=255)
                                                             tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                 >  1:2677843/1‑101 (MQ=255)
                                                             tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                 >  1:597921/1‑101 (MQ=255)
                                                             tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                 >  2:2634286/1‑101 (MQ=255)
                                                              cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                <  1:1248796/101‑1 (MQ=255)
                                                              cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                >  2:3436491/1‑101 (MQ=255)
                                                               gCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                               >  1:4195999/1‑101 (MQ=255)
                                                               cccTTTGCCGAAGCCCCTTCCTTCATCCTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                               <  1:3445579/100‑1 (MQ=255)
                                                                ccTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                              <  1:4125505/101‑1 (MQ=255)
                                                                 cTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                             >  2:352646/1‑101 (MQ=255)
                                                                  tttGCCGAAGCACCTTCCTTCGTCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                            >  1:2536377/1‑101 (MQ=255)
                                                                  tttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                            >  2:890825/1‑101 (MQ=255)
                                                                  tttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                            >  1:102214/1‑101 (MQ=255)
                                                                  tttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                            >  1:3917843/1‑101 (MQ=255)
                                                                    tGCCGAAGCACCTTCCTTCATCTTGCCGCAAAAG.GGGTTGGAGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                          >  1:1786610/1‑101 (MQ=255)
                                                                    tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                          >  1:1961955/1‑101 (MQ=255)
                                                                     gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                         >  1:120847/1‑101 (MQ=255)
                                                                     gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                         <  1:1518999/101‑1 (MQ=255)
                                                                       cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                       <  2:2807046/101‑1 (MQ=255)
                                                                       cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                       >  2:2002522/1‑101 (MQ=255)
                                                                       cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                       >  2:3185090/1‑101 (MQ=255)
                                                                         aaGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                     <  1:4362245/101‑1 (MQ=255)
                                                                         aaGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                     >  2:1396366/1‑101 (MQ=255)
                                                                         aaGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                     <  2:2387271/101‑1 (MQ=255)
                                                                         aaGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                     <  2:1536656/101‑1 (MQ=255)
                                                                             aCCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.a                >  2:4198614/1‑101 (MQ=255)
                                                                              ccttccttCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g              <  1:3327607/101‑1 (MQ=255)
                                                                              ccttccttCATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g              >  1:2687925/1‑101 (MQ=255)
                                                                               cttccttcATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGACGCCCCGGCCAGCGCGGAAAACGCC.A.g.g            >  2:3437572/1‑101 (MQ=255)
                                                                                ttccttcATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.gg           <  2:3780045/101‑2 (MQ=255)
                                                                                 tccttcATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggc          <  2:100720/101‑3 (MQ=255)
                                                                                  ccttcATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggca         >  2:2594256/1‑98 (MQ=255)
                                                                                   cttcATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggcag        <  1:2634457/101‑5 (MQ=255)
                                                                                     tcATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCagag       <  2:151283/101‑1 (MQ=255)
                                                                                      cATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAgg      <  2:1930869/101‑1 (MQ=255)
                                                                                      cATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAgg      >  1:2567866/1‑101 (MQ=255)
                                                                                      cATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAgg      >  1:1321788/1‑101 (MQ=255)
                                                                                      cATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAgg      >  2:2454755/1‑101 (MQ=255)
                                                                                      cATCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGCGATCAGACACGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.GAGCAGAgg      >  2:2994687/1‑101 (MQ=255)
                                                                                        tCTTGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGcc    <  1:3134425/101‑1 (MQ=255)
                                                                                          ttGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAA.G.GCAGAGGcccc  >  2:2805088/1‑101 (MQ=255)
                                                                                          ttGACG.AAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGcccc  >  2:1250330/1‑101 (MQ=255)
                                                                                                     ||                                                                                             
TGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCC.A.G.GCAGAGGCCCC  >  NC_014034/2177171‑2177354

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG