Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,313 +A 100% coding (64/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3131.→A100.0% 722.8 186coding (64/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (113/73):  ref base (0/0):  total (113/73)

Only 100 of 206 total aligned reads displayed.

CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GGGTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCC.AACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCG  >  NC_014034/2177218‑2177404
                                                                                                       |                                                                                                
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                                                                                   <  2:2716622/101‑1 (MQ=255)
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                                                                                   <  2:2614858/101‑1 (MQ=255)
 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                                                 >  2:2312113/1‑101 (MQ=255)
  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aa                                                                                                <  1:940063/101‑2 (MQ=255)
   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aag                                                                                               <  1:236947/101‑3 (MQ=255)
    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagc                                                                                              <  2:145783/101‑4 (MQ=255)
     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                                                                             >  2:413690/1‑97 (MQ=255)
      cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagccg                                                                                            >  2:1112506/1‑96 (MQ=255)
       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                                                                            >  1:1092019/1‑101 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGTCCCCGGTTTCGGCCGAAGCCGcccc                                                                                         >  2:1268592/1‑101 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                                                         <  1:2260084/101‑1 (MQ=255)
             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                                                      >  2:2505789/1‑101 (MQ=255)
              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                     <  1:1881689/101‑1 (MQ=255)
              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                     >  1:597921/1‑101 (MQ=255)
              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                     >  2:2634286/1‑101 (MQ=255)
              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                     >  2:3282516/1‑101 (MQ=255)
              tCGACTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                     <  1:317496/101‑1 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    <  1:1248796/101‑1 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    >  1:4163545/1‑101 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    >  2:3436491/1‑101 (MQ=255)
                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                                                   >  1:4195999/1‑101 (MQ=255)
                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                                                   >  2:359033/1‑101 (MQ=255)
                cccTTTGCCGAAGCCCCTTCCTTCATCCTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                                                   <  1:3445579/100‑1 (MQ=255)
                   tttGCCGAAGCACCTTCCTTCGTCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                                                >  1:2536377/1‑101 (MQ=255)
                   tttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                                                >  2:890825/1‑101 (MQ=255)
                    ttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                                                                               >  1:4118462/1‑101 (MQ=255)
                     tGCCGAAGCACCTTCCTTCATCTTGCCGCAAAAG.GGGTTGGAGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                                              >  1:1786610/1‑101 (MQ=255)
                      gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                                                             >  1:120847/1‑101 (MQ=255)
                      gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                                                             >  1:831021/1‑101 (MQ=255)
                         gAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                                                          >  1:2684529/1‑101 (MQ=255)
                          aaGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                                                         <  2:1536656/101‑1 (MQ=255)
                          aaGCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                                                         <  1:2722105/101‑1 (MQ=255)
                            gCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                                                       >  2:4372394/1‑101 (MQ=255)
                            gCACCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                                                       >  1:2937274/1‑101 (MQ=255)
                              aCCTTCCTTCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                     >  2:2366269/1‑101 (MQ=255)
                               ccttccttCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                   <  1:3327607/101‑1 (MQ=255)
                               ccttccttCATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                   <  1:1937434/101‑1 (MQ=255)
                                cttccttcATCTGGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                  <  2:2991251/101‑1 (MQ=255)
                                 ttccttcATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                 <  2:3780045/101‑2 (MQ=255)
                                 ttccttcATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                 >  2:317858/1‑100 (MQ=255)
                                   ccttcATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                               <  1:212437/101‑4 (MQ=255)
                                   ccttcATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                               >  2:1360483/1‑98 (MQ=255)
                                     ttcATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                             <  2:1577745/101‑6 (MQ=255)
                                      tcATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                             <  2:151283/101‑1 (MQ=255)
                                       cATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                            <  2:1930869/101‑1 (MQ=255)
                                       cATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                            >  2:2829472/1‑101 (MQ=255)
                                       cATCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                            >  1:2567866/1‑101 (MQ=255)
                                       cATCTTGACG.AAAAAGGGGTTGGTGTCGGCCTT.GGGGATCAGACACGCCCCGGTTTCGGACGAAGCCGCCCCCGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                            >  2:3387666/1‑101 (MQ=255)
                                         tCTTGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                         >  1:4206135/1‑101 (MQ=255)
                                           ttGACG.AAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                                                       >  2:1250330/1‑101 (MQ=255)
                                                      aggggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGcc                                             >  2:4254041/11‑101 (MQ=255)
                                                         ggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t                                        >  1:3422098/8‑101 (MQ=255)
                                                          gttggcgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.tt                                       >  2:828489/7‑101 (MQ=255)
                                                           ttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.ttt                                      <  2:4020530/96‑2 (MQ=255)
                                                           ttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGAAGAGGCCCCC.AACAGGGCCA.G.ttt                                      >  2:2422699/6‑100 (MQ=255)
                                                            tggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.tttg                                     <  2:733138/97‑3 (MQ=255)
                                                            tggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.tttg                                     <  1:2673767/97‑3 (MQ=255)
                                                            tggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.tttg                                     >  1:3614791/5‑99 (MQ=255)
                                                             ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.tttgc                                    >  2:1438379/4‑98 (MQ=255)
                                                              gtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.tttgcg                                   >  1:675556/3‑97 (MQ=255)
                                                              gtgtcggtcTTGGTGAT.CAGACAGGCCCCGGGTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.tttgcg                                   >  2:2926156/3‑97 (MQ=255)
                                                               tgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.tttgcgc                                  >  1:2640338/2‑96 (MQ=255)
                                                                gtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGca                                  >  2:2805215/1‑101 (MQ=255)
                                                                gtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGca                                  >  2:2463731/1‑101 (MQ=255)
                                                                gtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.CCCCTAACAGGGCCAGG.TTGCGca                                  >  1:4380214/1‑101 (MQ=255)
                                                                 tcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGcat                                 <  2:4118202/101‑1 (MQ=255)
                                                                  cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGcatc                                <  1:3785310/101‑1 (MQ=255)
                                                                      tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCatcatc                             >  2:2567001/11‑101 (MQ=255)
                                                                      tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCatcatc                             >  2:2447474/11‑101 (MQ=255)
                                                                      tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCatcatc                             <  2:2677671/91‑1 (MQ=255)
                                                                       cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCAtcatca                            >  2:242650/10‑101 (MQ=255)
                                                                       cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCAtcatca                            >  2:3147019/10‑101 (MQ=255)
                                                                       cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCAtcatca                            <  1:3262763/92‑1 (MQ=255)
                                                                        ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCAc                           <  1:3116696/93‑1 (MQ=255)
                                                                        ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCAc                           >  1:1573956/9‑101 (MQ=255)
                                                                         tggtgatcagacagGCCCCGGTTTCGTCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACg                          <  1:3733905/94‑1 (MQ=255)
                                                                           gtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGt                        >  2:2870893/6‑101 (MQ=255)
                                                                            tgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGtc                       >  1:1323705/5‑101 (MQ=255)
                                                                            tgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGtc                       >  1:3524286/5‑101 (MQ=255)
                                                                             gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGtct                      >  2:3550312/4‑101 (MQ=255)
                                                                             gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGtct                      >  2:3643108/4‑101 (MQ=255)
                                                                                cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGtctctc                   <  1:1002926/101‑1 (MQ=255)
                                                                                cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGtctctc                   <  1:3957878/101‑1 (MQ=255)
                                                                                 agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGTCCCA.GTTTGCGCCTCACCACGGTCTctcc                  >  1:1527164/1‑101 (MQ=255)
                                                                                 agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTctcc                  >  2:2891814/1‑101 (MQ=255)
                                                                                  gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTctcct                 >  2:2210230/1‑101 (MQ=255)
                                                                                  gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTctcct                 >  1:2834770/1‑101 (MQ=255)
                                                                                   acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTctcctc                <  2:2612827/101‑1 (MQ=255)
                                                                                    cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc               >  2:2643630/1‑101 (MQ=255)
                                                                                    cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc               >  1:1731441/1‑101 (MQ=255)
                                                                                    cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc               >  2:1623601/1‑101 (MQ=255)
                                                                                     agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc              >  2:36200/1‑101 (MQ=255)
                                                                                     agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc              <  2:3826782/101‑1 (MQ=255)
                                                                                      ggCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCg             <  1:198437/101‑1 (MQ=255)
                                                                                      ggCCCCGGTTTCGGACGAAGCCGCCCCGGCGAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCg             <  1:3387903/101‑1 (MQ=255)
                                                                                           cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa        <  1:4334797/101‑1 (MQ=255)
                                                                                            ggTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc       >  2:4216316/1‑101 (MQ=255)
                                                                                              tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg     >  2:1039917/1‑101 (MQ=255)
                                                                                               ttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt    >  2:3449799/1‑101 (MQ=255)
                                                                                                tCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTACACTGTc   <  2:2041351/101‑1 (MQ=255)
                                                                                                       |                                                                                                
CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GGGTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCC.AACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCG  >  NC_014034/2177218‑2177404

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG