Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,350 +C 100% coding (27/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3501.→C100.0% 666.0 179coding (27/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (84/94):  ref base (1/0):  total (85/94)

Only 100 of 200 total aligned reads displayed.

CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCC.A.G.GCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444
                                                                                                       |                                                                                               
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAgg                                                                                                  <  2:1930869/101‑1 (MQ=255)
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAgg                                                                                                  >  2:2454755/1‑101 (MQ=255)
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGCGATCAGACACGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.GAGCAGAgg                                                                                                  >  2:2994687/1‑101 (MQ=255)
  tCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGG.cc                                                                                               <  1:3134425/101‑1 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAA.G.GCAGAGG.cccc                                                                                             >  2:2805088/1‑101 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGG.cccc                                                                                             >  2:4120773/1‑101 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGG.cccc                                                                                             >  2:1250330/1‑101 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAAGCC.AGG.GAAGAGG.cccc                                                                                             >  2:1268488/1‑101 (MQ=255)
              aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGcc                                                                                    <  2:3177994/91‑1 (MQ=255)
               ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCa                                                                                   <  1:3982629/92‑1 (MQ=255)
                 ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.t                                                                                >  1:3422098/8‑101 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.ttt                                                                              <  1:365333/96‑2 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttg                                                                             >  2:1528965/5‑99 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttg                                                                             <  2:1584331/97‑3 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttg                                                                             <  1:2673767/97‑3 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttg                                                                             <  2:733138/97‑3 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                            >  2:1438379/4‑98 (MQ=255)
                      gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                           <  1:4229949/99‑5 (MQ=255)
                       tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                                                          >  2:1447226/2‑96 (MQ=255)
                       tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                                                          >  1:665754/2‑96 (MQ=255)
                         tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                                         <  2:2880550/101‑1 (MQ=255)
                         tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                                         <  2:876534/101‑1 (MQ=255)
                          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                                        <  1:3785310/101‑1 (MQ=255)
                             tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                                     >  2:2567001/11‑101 (MQ=255)
                                   tgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                               >  1:1323705/5‑101 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              <  2:2353748/98‑1 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              <  2:2684339/98‑1 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              <  2:340980/98‑1 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              >  2:3550312/4‑101 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              >  2:3643108/4‑101 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              <  1:4198875/98‑1 (MQ=255)
                                       cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                                                           <  1:1002926/101‑1 (MQ=255)
                                         gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                                                         >  1:2441577/1‑101 (MQ=255)
                                         gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                                                         >  2:2210230/1‑101 (MQ=255)
                                         gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                                                         >  1:2834770/1‑101 (MQ=255)
                                         gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                                                         <  1:724436/101‑1 (MQ=255)
                                          acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                                        <  2:2612827/101‑1 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       >  2:1623601/1‑101 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       >  2:2643630/1‑101 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  2:2426434/101‑1 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  2:3826782/101‑1 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  1:1885033/1‑101 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  1:155635/1‑101 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  2:36200/1‑101 (MQ=255)
                                             ggCCCCGGTTTCGGACGAAGCCGCCCCGGCGAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                                     <  1:3387903/101‑1 (MQ=255)
                                               ccccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt                                                   <  2:2868255/101‑1 (MQ=255)
                                                   ggTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc                                               >  2:4216316/1‑101 (MQ=255)
                                                     tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                             <  1:2331628/101‑1 (MQ=255)
                                                     tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                             <  1:2959772/101‑1 (MQ=255)
                                                      ttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                            <  1:3611355/101‑1 (MQ=255)
                                                       tCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTACACTGTc                                           <  2:2041351/101‑1 (MQ=255)
                                                        cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                          <  1:2805421/101‑1 (MQ=255)
                                                           ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                                       >  2:4187774/5‑101 (MQ=255)
                                                           ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                                       >  1:82427/5‑101 (MQ=255)
                                                           ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                                       <  1:352681/97‑1 (MQ=255)
                                                            cgaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                      >  2:3351704/4‑101 (MQ=255)
                                                             gaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     <  1:3113425/99‑1 (MQ=255)
                                                              aaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                                    <  1:3056185/100‑1 (MQ=255)
                                                              aaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                                    <  2:2741032/100‑1 (MQ=255)
                                                               aGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctc                                   <  1:2875672/101‑1 (MQ=255)
                                                                gccgccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                                  >  1:4352538/1‑101 (MQ=255)
                                                                   gccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               >  2:518173/1‑101 (MQ=255)
                                                                   gccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               <  1:3185317/101‑1 (MQ=255)
                                                                      ccGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                            >  1:1305332/1‑101 (MQ=255)
                                                                      ccGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                            <  1:1447333/101‑1 (MQ=255)
                                                                      ccGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                            <  2:106398/101‑1 (MQ=255)
                                                                        ggCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                          >  2:1174779/1‑101 (MQ=255)
                                                                        ggCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                          >  2:639835/1‑101 (MQ=255)
                                                                         gCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         >  1:2580128/1‑101 (MQ=255)
                                                                          ccAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                        >  2:4027966/1‑101 (MQ=255)
                                                                           cAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                       >  2:3080982/1‑101 (MQ=255)
                                                                           cAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                       <  1:2454912/101‑1 (MQ=255)
                                                                            aGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg                      <  1:3437694/101‑1 (MQ=255)
                                                                               gcgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACa                   <  2:102193/101‑1 (MQ=255)
                                                                                cgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa                  >  1:1525836/1‑101 (MQ=255)
                                                                                cgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa                  <  1:3950109/101‑1 (MQ=255)
                                                                                cgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCCGGGCTCGGACaa                  <  1:2422856/101‑1 (MQ=255)
                                                                                 ggAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 >  1:4442928/1‑101 (MQ=255)
                                                                                 ggAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 <  1:363778/101‑1 (MQ=255)
                                                                                  gAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGCGCTCGGACAAAg                >  2:3984061/1‑101 (MQ=255)
                                                                                   aaaaCGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt               <  1:2027648/101‑1 (MQ=255)
                                                                                   aaaaCGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt               <  2:2465472/101‑1 (MQ=255)
                                                                                    aaaCGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAgcc              >  1:3367961/1‑99 (MQ=255)
                                                                                      aCGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            <  1:163939/101‑1 (MQ=255)
                                                                                      aCGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            <  2:1323608/101‑1 (MQ=255)
                                                                                           cagg.gCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc        >  2:941093/4‑101 (MQ=255)
                                                                                           cagg.gCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc        <  2:2441420/98‑1 (MQ=255)
                                                                                           cagg.gCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc        <  2:3802315/98‑1 (MQ=255)
                                                                                            agg.gCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg       <  2:155614/99‑1 (MQ=255)
                                                                                              g.gCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     <  2:3524038/101‑1 (MQ=255)
                                                                                              g.gCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     <  1:3147243/101‑1 (MQ=255)
                                                                                              g.gCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     >  1:1768613/1‑101 (MQ=255)
                                                                                                gCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGt    <  2:2640167/101‑1 (MQ=255)
                                                                                                 cAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   <  2:2901591/101‑1 (MQ=255)
                                                                                                 cAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   >  2:630971/1‑101 (MQ=255)
                                                                                                 cAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   <  1:4340786/101‑1 (MQ=255)
                                                                                                 agaggg.CCCCAACAGGGCCAGTTTGCGCATCATCACGGGCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGAGGCTCGGACAAAGACTGACCCGAGtag  >  2:2936095/5‑99 (MQ=255)
                                                                                                  agagGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  2:2811534/1‑101 (MQ=255)
                                                                                                  agagGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  <  2:1213504/101‑1 (MQ=255)
                                                                                                  agagGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  1:3470315/1‑101 (MQ=255)
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CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCC.A.G.GCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG