Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,365 +T 100% coding (12/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3651.→T100.0% 756.1 192coding (12/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (88/103):  ref base (0/1):  total (88/104)

Only 100 of 215 total aligned reads displayed.

AGGGTTGGTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCC.AACAGGGCCA.G.T.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAATAT  >  NC_014034/2177270‑2177460
                                                                                                      |                                                                                                
ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t                                                                                                 >  1:3422098/8‑101 (MQ=255)
 gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.t                                                                                               >  2:2641597/7‑101 (MQ=255)
 gttggtggcggtcTTGGTGATCAGACAGGCCCCGGTTTCGACCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCAA.G.t.t                                                                                               <  2:132175/93‑1 (MQ=255)
  ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGAAGAGGCCCCC.AACAGGGCCA.G.t.tt                                                                                              >  2:2422699/6‑100 (MQ=255)
   tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttg                                                                                             <  2:733138/97‑3 (MQ=255)
   tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttg                                                                                             <  1:2673767/97‑3 (MQ=255)
   tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttg                                                                                             >  1:3614791/5‑99 (MQ=255)
    ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttgc                                                                                            >  2:1438379/4‑98 (MQ=255)
     gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttgcg                                                                                           <  1:4229949/99‑5 (MQ=255)
     gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttgcg                                                                                           >  1:675556/3‑97 (MQ=255)
     gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttgcg                                                                                           >  1:211097/3‑97 (MQ=255)
      tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttgcgc                                                                                          >  1:2640338/2‑96 (MQ=255)
      tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.t.ttgcgc                                                                                          >  2:1447226/2‑96 (MQ=255)
       gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.CCCCTAACAGGGCCAGG.T.TGCGca                                                                                          >  1:4380214/1‑101 (MQ=255)
        tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGcat                                                                                         <  2:2880550/101‑1 (MQ=255)
        tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGcat                                                                                         <  2:4118202/101‑1 (MQ=255)
        tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGcat                                                                                         <  2:597853/101‑1 (MQ=255)
         cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGcatc                                                                                        <  1:3785310/101‑1 (MQ=255)
            tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCatcatc                                                                                     >  2:2447474/11‑101 (MQ=255)
            tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCatcatc                                                                                     <  2:2677671/91‑1 (MQ=255)
             cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCAtcatca                                                                                    <  1:3262763/92‑1 (MQ=255)
             cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCAtcatca                                                                                    >  2:3147019/10‑101 (MQ=255)
             cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCAtcatca                                                                                    >  2:242650/10‑101 (MQ=255)
              ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCAc                                                                                   <  1:3116696/93‑1 (MQ=255)
              ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCAc                                                                                   >  1:1573956/9‑101 (MQ=255)
                  tgatcagacagCCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGtc                                                                               <  1:3436730/97‑1 (MQ=255)
                   gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGtct                                                                              <  2:2353748/98‑1 (MQ=255)
                   gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGtct                                                                              >  2:3550312/4‑101 (MQ=255)
                   gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGtct                                                                              >  2:3643108/4‑101 (MQ=255)
                      cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGtctctc                                                                           <  1:1002926/101‑1 (MQ=255)
                       agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGTCCCA.GTT.TGCGCCTCACCACGGTCTctcc                                                                          >  1:1527164/1‑101 (MQ=255)
                        gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTctcct                                                                         >  2:2210230/1‑101 (MQ=255)
                        gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTctcct                                                                         >  1:2834770/1‑101 (MQ=255)
                          cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCtcctcc                                                                       >  2:1623601/1‑101 (MQ=255)
                           agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTccc                                                                      >  2:36200/1‑101 (MQ=255)
                           agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTccc                                                                      <  2:3826782/101‑1 (MQ=255)
                            ggCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCg                                                                     <  2:922949/101‑1 (MQ=255)
                            ggCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCg                                                                     >  1:336838/1‑101 (MQ=255)
                            ggCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCg                                                                     <  1:198437/101‑1 (MQ=255)
                              ccccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGtt                                                                   <  2:2868255/101‑1 (MQ=255)
                                 cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                                >  2:2613222/1‑101 (MQ=255)
                                    tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                                             <  1:2959772/101‑1 (MQ=255)
                                       cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                                          <  2:1573851/101‑1 (MQ=255)
                                          ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                                                       <  2:2702092/97‑1 (MQ=255)
                                          ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                                                       <  1:352681/97‑1 (MQ=255)
                                            gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     >  1:1522968/3‑101 (MQ=255)
                                            gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     >  2:1546063/3‑101 (MQ=255)
                                            gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     <  2:1231221/99‑1 (MQ=255)
                                            gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                                     >  2:255525/3‑101 (MQ=255)
                                             aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                                                    >  1:17692/2‑101 (MQ=255)
                                               gccgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                                                  >  1:4352538/1‑101 (MQ=255)
                                               gccgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                                                  >  1:2348501/1‑101 (MQ=255)
                                                 cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                                <  1:762365/101‑1 (MQ=255)
                                                  gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                                               <  2:4163284/101‑1 (MQ=255)
                                                  gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                                               >  2:637716/1‑101 (MQ=255)
                                                   ccccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                                              <  2:1522863/101‑1 (MQ=255)
                                                     ccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                                            <  2:106398/101‑1 (MQ=255)
                                                     ccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                                            <  1:1447333/101‑1 (MQ=255)
                                                     ccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                                            >  1:1305332/1‑101 (MQ=255)
                                                       ggCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                                          >  2:1174779/1‑101 (MQ=255)
                                                        gCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                                         <  1:3643361/101‑1 (MQ=255)
                                                        gCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                                         >  1:2580128/1‑101 (MQ=255)
                                                         ccAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                                        >  2:4027966/1‑101 (MQ=255)
                                                          cAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                                       >  2:2038936/1‑101 (MQ=255)
                                                            gcgcGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGa                                     <  2:2567703/101‑1 (MQ=255)
                                                              gcgGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGCCa                                   >  1:306880/1‑101 (MQ=255)
                                                              gcgGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACa                                   <  1:636294/101‑1 (MQ=255)
                                                              gcgGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACa                                   <  2:102193/101‑1 (MQ=255)
                                                               cgGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa                                  <  1:2447631/101‑1 (MQ=255)
                                                               cgGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa                                  <  1:3950109/101‑1 (MQ=255)
                                                                ggAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                                 <  1:3519170/101‑1 (MQ=255)
                                                                 gAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGCGCTCGGACAAAg                                >  2:3984061/1‑101 (MQ=255)
                                                                     aCGCCAGGGCAGAGGCCCCC.AACAGGGCCA.G.TGTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg                            <  2:4205874/101‑1 (MQ=255)
                                                                         cagggCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc                        >  2:2114721/4‑101 (MQ=255)
                                                                            ggCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg                     <  1:3147243/101‑1 (MQ=255)
                                                                            ggCAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg                     <  2:3524038/101‑1 (MQ=255)
                                                                              cAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc                   <  1:4340786/101‑1 (MQ=255)
                                                                              cAGAGGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc                   <  2:2901591/101‑1 (MQ=255)
                                                                              agaggg.CCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGGCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGAGGCTCGGACAAAGACTGACCCGAGtag                  >  2:2936095/5‑99 (MQ=255)
                                                                               agagGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg                  <  2:3007989/101‑1 (MQ=255)
                                                                               agagGCCCCC.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg                  >  2:279542/1‑101 (MQ=255)
                                                                                 aggccccc.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgc                >  1:2865360/5‑101 (MQ=255)
                                                                                 aggccccc.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgc                <  2:830939/97‑1 (MQ=255)
                                                                                  ggccccc.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGgcg               <  1:2039073/98‑1 (MQ=255)
                                                                                      ccc.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGcccc           <  2:694590/101‑1 (MQ=255)
                                                                                       cc.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCg          >  1:2980309/1‑101 (MQ=255)
                                                                                        c.AACAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCgg         <  2:3421859/101‑1 (MQ=255)
                                                                                        c.AACAGGGCCA.GTT.TGCGCATCATCACGGGCTCTCCTCCCGTTTTACTGTCTGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCgg         >  2:3673860/1‑101 (MQ=255)
                                                                                          aaCAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAACGTCTGACCCCAGTCGGCGCCCCggg        >  1:2029229/1‑100 (MQ=255)
                                                                                           aCAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTc       <  1:3437811/101‑1 (MQ=255)
                                                                                            cAGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCa      <  2:454001/101‑1 (MQ=255)
                                                                                             aGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCaa     <  1:4121033/101‑1 (MQ=255)
                                                                                             aGGGCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCaa     >  1:2770448/1‑101 (MQ=255)
                                                                                              gggCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAat    <  1:1760534/101‑1 (MQ=255)
                                                                                              gggCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAat    >  2:128995/1‑101 (MQ=255)
                                                                                               ggCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAata   <  2:120826/101‑1 (MQ=255)
                                                                                               ggCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAata   >  2:3369262/1‑101 (MQ=255)
                                                                                               ggCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAata   >  1:2433078/1‑101 (MQ=255)
                                                                                               ggCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAata   <  1:1360580/101‑1 (MQ=255)
                                                                                                gCCA.GTT.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAatat  <  1:3711275/101‑1 (MQ=255)
                                                                                                      |                                                                                                
AGGGTTGGTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCC.AACAGGGCCA.G.T.TGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCGGCGCCCCGGTCAATAT  >  NC_014034/2177270‑2177460

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG