Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,930	+C	100%	intergenic (+203/‑302)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,930	1	.→C	100.0%	167.2	47	intergenic (+203/‑302)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (20/26):  ref base (0/1):  total (20/27)

TGCGGGGGCTCCG.CCCC.GCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAG  >  NC_014034/2185829‑2186021                                                                                                         |                                                                                              tGCGGGGGCTCCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGGAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCg                                                                                                <  2:1059854/101‑1 (MQ=25)  gCGGGGGCTCCG.CCCCAGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.c                                                                                              >  2:1030281/1‑101 (MQ=37)   cGGGGGCTCCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGACGAACCG.cc                                                                                             >  2:778582/1‑101 (MQ=25)    gggggCTCCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccc                                                                                            <  1:2844461/101‑1 (MQ=37)          tccgccccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctttc                                                                                      <  1:1933459/96‑3 (MQ=37)           ccgccccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCa                                                                                      <  2:815708/97‑1 (MQ=37)               cccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCtt                                                                                  <  1:571069/101‑1 (MQ=38)                ccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTg                                                                                 <  2:4164453/101‑1 (MQ=38)                ccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTg                                                                                 <  1:869726/101‑1 (MQ=38)                     gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCt                                                                              <  2:392060/98‑1 (MQ=38)                      cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTc                                                                             >  1:2919293/3‑101 (MQ=39)                       ccccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCCCCTTGGCTCa                                                                            <  2:2980303/101‑1 (MQ=38)                       acccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCa                                                                            >  1:175090/2‑101 (MQ=255)                        cccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCaa                                                                           <  1:788341/101‑1 (MQ=255)                          cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAat                                                                         <  1:1137702/101‑1 (MQ=255)                          cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAat                                                                         <  2:2032482/101‑1 (MQ=255)                           ccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTCATGGTACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCCAata                                                                        >  2:1769438/1‑101 (MQ=255)                            cGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAatat                                                                       <  1:2021392/101‑1 (MQ=255)                             ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATc                                                                      <  1:3464580/101‑1 (MQ=255)                             ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATc                                                                      <  1:2033760/101‑1 (MQ=255)                              gCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATcc                                                                     <  1:319908/101‑1 (MQ=255)                               cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATccc                                                                    >  1:2301058/1‑101 (MQ=255)                               cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATccc                                                                    >  2:1196191/1‑101 (MQ=255)                                gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCg                                                                   <  1:2371912/101‑1 (MQ=255)                                gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCg                                                                   <  1:3264854/101‑1 (MQ=255)                                 tATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCgg                                                                  <  1:4145593/101‑1 (MQ=255)                                  aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCggg                                                                 >  1:697829/1‑101 (MQ=255)                                    ttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCggggg                                                               >  1:2823282/1‑101 (MQ=255)                                      aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCAGAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTc                                                             >  1:3764180/1‑101 (MQ=38)                                      aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTc                                                             <  1:2276389/101‑1 (MQ=255)                                      aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTc                                                             >  2:3410970/1‑101 (MQ=255)                                       tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                            >  2:815041/1‑101 (MQ=255)                                       tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                            >  2:4077041/1‑101 (MQ=255)                                       tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                            <  1:2583308/101‑1 (MQ=255)                                       tCCCAAGAAAAAGCAGCGGCTTGCCCCCAATGCCTTG.TTTTGATGGCACAAGAAACGCCCAACCGCCCCCTTTCACCTTGGCTCAAATATCCCgcgggcgc                                                            >  2:4387500/1‑98 (MQ=255)                                        cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                                                           <  1:3946348/101‑1 (MQ=255)                                        cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                                                           <  2:4008519/101‑1 (MQ=255)                                          cAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCggg                                                         <  2:3658013/101‑1 (MQ=255)                                            aGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCggggg                                                       >  1:54756/1‑101 (MQ=255)                                             gAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAAGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCCGGGGTCCGGGGGc                                                      >  2:2159751/1‑101 (MQ=255)                                              aaaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCg                                                     <  2:3077307/101‑1 (MQ=255)                                               aaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCgg                                                    >  1:11863/1‑101 (MQ=255)                                                aaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGa                                                   <  1:1269312/101‑1 (MQ=255)                                                 aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAg                                                  <  2:4252996/101‑1 (MQ=255)                                                   gcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cc                                              >  2:1542320/1‑101 (MQ=255)                                                     agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccc                                            >  2:3171484/1‑100 (MQ=37)                                                      gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..ccccc                                           >  1:3410807/1‑99 (MQ=37)                                                        ggCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccccgg                                         >  2:2370979/1‑97 (MQ=25)                                                             gCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccccggcgggg                                    >  2:2179669/1‑92 (MQ=21)                                                              ccccGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccccggcggggg                                   >  2:3248286/1‑91 (MQ=21)                                                              ccccGAAGGCCTTG.TTTTGATGGCAAAAGAACCGCCGACCCGCCCCCTTTCCCCTTGGCTCAAATATCCGGGGGGTCGGGGGGCGGAG..cccccggcgggtc                                   <  2:1734320/101‑11 (MQ=16)                                                                                                gCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAg  <  1:778662/101‑1 (MQ=2)                                                                                                         |                                                                                              TGCGGGGGCTCCG.CCCC.GCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAG  >  NC_014034/2185829‑2186021 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG