BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,249,280	+C	97.8%	intergenic (‑76/‑229)	parE ← / → RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,249,280	1	.→C	97.8%	4.1 ‑0.5 ‑0.3	180	intergenic (‑76/‑229)	parE/RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): new base (76/99): ref base (1/3): total (78/102)
Fisher's exact test strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.55e-01

Only 100 of 225 total aligned reads displayed.


TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAG  >  NC_014034/2249180‑2249375


tCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTg                                                                                                  >  2:4311191/1‑101 (MQ=255)


tCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTg                                                                                                  <  1:2314711/101‑1 (MQ=255)


 cGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.c                                                                                                >  2:79752/1‑101 (MQ=255)


  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                               >  1:1725852/1‑101 (MQ=255)


    ggACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccc                                                                                             <  2:1687001/101‑1 (MQ=255)


     gACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccccc                                                                                            <  2:2030543/101‑2 (MQ=255)


     gACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccccc                                                                                            <  2:3692096/101‑2 (MQ=255)


      aCAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccg                                                                                           >  2:1006389/1‑99 (MQ=255)


       cAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccgt                                                                                          >  2:4246705/1‑98 (MQ=255)


       cAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccgt                                                                                          >  1:70312/1‑98 (MQ=255)


        agaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccgtc                                                                                         >  1:942127/1‑97 (MQ=255)


        agaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccgtc                                                                                         <  1:522564/101‑5 (MQ=255)


         ggagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccgtcc                                                                                        <  1:648305/99‑6 (MQ=255)


           agaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcccgtcccg                                                                                       <  1:2237838/101‑1 (MQ=255)


            gaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcccgtcccgt                                                                                      <  2:4395529/101‑1 (MQ=255)


             aTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTg                                                                                     >  2:2463592/1‑101 (MQ=255)


             aTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTg                                                                                     <  1:2623154/101‑1 (MQ=255)


              tCGGCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATCTACAGATACAGCAGCTTGGGCGCCCGGGGCCAGAAGGGGGCTCTGCCCCCGCCCCGTGc                                                                                    >  2:2279193/1‑101 (MQ=255)


               cGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGcc                                                                                   <  1:1865528/101‑1 (MQ=255)


               cGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGcc                                                                                   <  1:3349868/101‑1 (MQ=255)


                gCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCg                                                                                  <  2:3649825/101‑1 (MQ=255)


                 ccGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCgg                                                                                 <  2:1880244/101‑1 (MQ=255)


                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCggg                                                                                >  2:2800941/1‑101 (MQ=255)


                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCggg                                                                                >  2:4358801/1‑101 (MQ=255)


                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCggg                                                                                >  2:3155967/1‑101 (MQ=255)


                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCggg                                                                                <  2:1114046/101‑1 (MQ=255)


                   ggACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGa                                                                               >  1:3320795/1‑101 (MQ=255)


                   ggACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGa                                                                               >  2:3740112/1‑101 (MQ=255)


                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTcccc                                                                         <  1:2696750/101‑1 (MQ=255)


                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTcccc                                                                         >  2:499698/1‑101 (MQ=255)


                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTcccc                                                                         <  1:3252008/101‑1 (MQ=255)


                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTccccc                                                                        <  2:3989280/101‑1 (MQ=255)


                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTccccc                                                                        <  2:181496/101‑1 (MQ=255)


                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTccccc                                                                        <  1:4150036/101‑1 (MQ=255)


                          gTGCTCGCTGTCATTGGTGTTCTTGGGATGCCCAGATACCGCAGCTTGGGCACGCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTccccc                                                                        <  1:1060722/101‑1 (MQ=255)


                           tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCg                                                                       <  2:2477926/101‑1 (MQ=255)


                            tcTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCgg                                                                      <  2:1396408/100‑1 (MQ=255)


                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTGTGCCCCCGTCCCCGGCCGGGACTCCCCCgg                                                                      >  2:707848/1‑101 (MQ=255)


                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCgg                                                                      <  1:509079/101‑1 (MQ=255)


                                gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGata                                                                  >  2:1240739/1‑101 (MQ=255)


                                gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGata                                                                  <  2:1649251/101‑1 (MQ=255)


                                gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGata                                                                  <  1:4109733/101‑1 (MQ=255)


                                gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGata                                                                  <  2:3275736/101‑1 (MQ=255)


                                gCTTTCATTGGTGTTCTTGAGATGCACAGAGACCGCAGCTTGGGCACCCGGGGCAAGAAGCGGGCTCTGCCCCCGTCCCGCGCCGGGACTCCCCCGGGata                                                                  >  2:3715717/1‑101 (MQ=255)


                                 cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGatat                                                                 <  1:2804579/101‑1 (MQ=255)


                                 cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGatat                                                                 >  2:2119572/1‑101 (MQ=255)


                                 cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGatat                                                                 >  1:3529912/1‑101 (MQ=255)


                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGTAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                <  1:3817492/101‑1 (MQ=255)


                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                <  1:2345954/101‑1 (MQ=255)


                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                >  2:3474304/1‑101 (MQ=255)


                                   ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATAttt                                                               <  1:830629/101‑1 (MQ=255)


                                    tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                              >  1:3983218/1‑101 (MQ=255)


                                    tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                              <  1:3904895/101‑1 (MQ=255)


                                      aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTgcg                                                            <  2:2385796/101‑3 (MQ=255)


                                      aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAg                                                            >  1:1451835/1‑101 (MQ=255)


                                          gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCaa                                                        >  1:4231972/1‑101 (MQ=255)


                                          gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCaa                                                        <  1:219015/101‑1 (MQ=255)


                                           tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGCGACTCCCCCGGGATATTTGAGGCAAg                                                       >  2:732493/1‑101 (MQ=255)


                                            gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGa                                                      >  2:1084230/1‑101 (MQ=255)


                                            gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGa                                                      <  2:2353235/101‑1 (MQ=255)


                                            gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGa                                                      >  1:1188023/1‑101 (MQ=255)


                                            gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGAAAGa                                                      >  1:1700713/1‑101 (MQ=255)


                                             ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGAt                                                     >  2:418850/1‑101 (MQ=255)


                                             ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGAt                                                     <  2:515801/101‑1 (MQ=255)


                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    <  1:4240242/101‑1 (MQ=255)


                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    <  1:4378128/101‑1 (MQ=255)


                                               cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                   <  1:296014/101‑1 (MQ=255)


                                               cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                   >  2:4369472/1‑101 (MQ=255)


                                                ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGaa                                                  <  2:272470/101‑1 (MQ=255)


                                                    gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGGAAGATGAAgggg                                              >  2:2608384/1‑101 (MQ=255)


                                                     aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAggggg                                             <  1:906413/101‑1 (MQ=255)


                                                      tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgggggg                                            <  1:3768299/101‑1 (MQ=255)


                                                       gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGc                                           <  1:431239/101‑1 (MQ=255)


                                                        cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCt                                          <  2:2499306/101‑1 (MQ=255)


                                                           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                       <  1:4216614/101‑1 (MQ=255)


                                                           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                       <  1:1594615/101‑1 (MQ=255)


                                                            gATACCGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCa                                      <  1:3614013/101‑1 (MQ=255)


                                                            gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCa                                      >  1:174611/1‑101 (MQ=255)


                                                            gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCa                                      >  2:2338390/1‑101 (MQ=255)


                                                              tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATc                                    <  1:2571701/101‑1 (MQ=255)


                                                              tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATc                                    >  2:1570295/1‑101 (MQ=255)


                                                               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                   >  1:588565/1‑101 (MQ=255)


                                                                 cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTg                                 <  2:1451728/101‑1 (MQ=255)


                                                                  gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGc                                <  1:1359721/101‑1 (MQ=255)


                                                                   cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGcc                               >  2:1457976/1‑101 (MQ=255)


                                                                    aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                              <  2:905584/101‑1 (MQ=255)


                                                                    aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                              >  1:239901/1‑101 (MQ=255)


                                                                       ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaa                           <  2:4225319/101‑1 (MQ=255)


                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                         <  2:4468/101‑1 (MQ=255)


                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                         >  2:625976/1‑101 (MQ=255)


                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                         >  1:1337162/1‑101 (MQ=255)


                                                                          ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAata                        <  1:3156191/101‑1 (MQ=255)


                                                                            cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATc                      >  2:3232114/1‑101 (MQ=255)


                                                                             aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATcc                     <  1:2418784/101‑1 (MQ=255)


                                                                                cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgg                  >  1:1909028/1‑101 (MQ=255)


                                                                                    gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg              >  2:1158809/1‑101 (MQ=255)


                                                                                          agGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg        <  2:1526650/101‑1 (MQ=255)


                                                                                               gCTCTGCCCCCGTCCGGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGTCTTTTCATGTTGCCGAAACTATCCCGGTCGCTGAATGgcggc   >  2:295579/1‑99 (MQ=21)


                                                                                               gCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa   <  2:3436257/101‑1 (MQ=255)


                                                                                               gCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa   <  1:1006474/101‑1 (MQ=255)


TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAG  >  NC_014034/2249180‑2249375

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG