Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,300 +C 5.1% intergenic (‑96/‑209) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3001.→C 5.1% 21.2 ‑1.2 ‑0.0177intergenic (‑96/‑209)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (2/7):  ref base (75/93):  total (77/100)
Fisher's exact test strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-01

Only 100 of 205 total aligned reads displayed.

CATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249202‑2249400
                                                                                                     |                                                                                                       
   ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.cccc                                                                                                     <  1:3252008/101‑1 (MQ=255)
   ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.cccc                                                                                                     >  2:499698/1‑101 (MQ=255)
    gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.ccccc                                                                                                    <  2:3989280/101‑1 (MQ=255)
     tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCg                                                                                                   <  2:2477926/101‑1 (MQ=255)
      tcTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCgg                                                                                                  <  2:1396408/100‑1 (MQ=255)
      gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTGTGCCC.CCGTCCCCGGCCGGGACT.CCCCCgg                                                                                                  >  2:707848/1‑101 (MQ=255)
      gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCgg                                                                                                  >  1:951100/1‑101 (MQ=255)
        tCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGa                                                                                                <  1:299773/101‑1 (MQ=255)
         cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGat                                                                                               <  1:687020/101‑1 (MQ=255)
         cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGat                                                                                               >  2:591856/1‑101 (MQ=255)
          gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              <  2:3275736/101‑1 (MQ=255)
          gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              <  1:4172209/101‑1 (MQ=255)
          gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              >  2:1240739/1‑101 (MQ=255)
          gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              <  2:1649251/101‑1 (MQ=255)
          gCTTTCATTGGTGTTCTTGAGATGCACAGAGACCGCAGCTTGGGCACCCGGGGCAAGAAGCGGGCTCTGCCC.CCGTCCCGCGCCGGGACT.CCCCCGGGata                                                                                              >  2:3715717/1‑101 (MQ=255)
          gCTTTCATTGGTATTCTTGAGAGGCACAGATACCGGAGCTTGGGCACCCGGGGCAATAAGGGGCCTCTG.CCTCCGTCCCGTGCCGCGACT.CCCCCGGGacg                                                                                              >  2:2938409/1‑99 (MQ=255)
           cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGatat                                                                                             <  1:2804579/101‑1 (MQ=255)
           cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGatat                                                                                             >  2:2119572/1‑101 (MQ=255)
           cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGatat                                                                                             >  1:3529912/1‑101 (MQ=255)
            tttCATTGGTGTTCTTGAGATGCACAGATACCGTAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            <  1:3817492/101‑1 (MQ=255)
            tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            >  2:3474304/1‑101 (MQ=255)
            tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            <  2:1723643/101‑1 (MQ=255)
            tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            >  1:1976586/1‑101 (MQ=255)
            tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            >  1:4129362/1‑101 (MQ=255)
             ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           >  2:513543/1‑101 (MQ=255)
              tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          <  2:3940937/101‑1 (MQ=255)
                aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                        >  1:1451835/1‑101 (MQ=255)
                 ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                       >  2:1806592/1‑101 (MQ=255)
                  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCTCCGGGATATTTGAGGc                                                                                      >  2:241720/1‑101 (MQ=255)
                  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      <  1:3902155/101‑1 (MQ=255)
                  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      >  1:2410246/1‑101 (MQ=255)
                   ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGGCT.CCCCCGGGATATTTGAGGCa                                                                                     <  2:3637114/101‑1 (MQ=255)
                   ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                     <  1:3582859/101‑1 (MQ=255)
                    gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                    <  1:219015/101‑1 (MQ=255)
                    gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                    >  1:4231972/1‑101 (MQ=255)
                      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                  <  2:2353235/101‑1 (MQ=255)
                      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                  >  2:1084230/1‑101 (MQ=255)
                      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                  >  1:1188023/1‑101 (MQ=255)
                      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGa                                                                                  >  1:1700713/1‑101 (MQ=255)
                        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                >  1:3338921/1‑101 (MQ=255)
                        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                <  1:4378128/101‑1 (MQ=255)
                         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                               <  1:3945259/101‑1 (MQ=255)
                         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                               >  2:4369472/1‑101 (MQ=255)
                          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                              <  2:272470/101‑1 (MQ=255)
                          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                              <  1:2292102/101‑1 (MQ=255)
                             agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                        >  2:83736/1‑101 (MQ=255)
                                 gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGc                                                                    <  2:2203615/101‑1 (MQ=255)
                                  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                   <  2:2499306/101‑1 (MQ=255)
                                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                 <  1:896766/101‑1 (MQ=255)
                                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                <  1:1594615/101‑1 (MQ=255)
                                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                <  1:4216614/101‑1 (MQ=255)
                                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                               >  1:174611/1‑101 (MQ=255)
                                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                               <  2:1854648/101‑1 (MQ=255)
                                       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCACGATGAA...GGGGGGCTTTCAt                                                              >  1:2654751/1‑101 (MQ=255)
                                        tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                             >  2:1570295/1‑101 (MQ=255)
                                        tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                             <  1:2571701/101‑1 (MQ=255)
                                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                            >  1:907763/1‑101 (MQ=255)
                                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                            >  1:588565/1‑101 (MQ=255)
                                          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                           <  2:4263174/101‑1 (MQ=255)
                                          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                           <  1:3744580/101‑1 (MQ=255)
                                           cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                          <  2:1451728/101‑1 (MQ=255)
                                            gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGc                                                         <  1:1359721/101‑1 (MQ=255)
                                             cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGcc                                                        >  2:1457976/1‑101 (MQ=255)
                                                cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                     <  1:758475/101‑1 (MQ=255)
                                                 ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaa                                                    <  2:4225319/101‑1 (MQ=255)
                                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTGATCTTGCGGaaaa                                                   >  2:439733/1‑101 (MQ=38)
                                                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  <  2:4468/101‑1 (MQ=255)
                                                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  >  2:625976/1‑101 (MQ=255)
                                                      cACCCGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                               >  2:3232114/1‑101 (MQ=255)
                                                         ccGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                            >  1:2346695/1‑101 (MQ=255)
                                                         ccGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                            >  1:4321817/1‑101 (MQ=255)
                                                          cGGGGCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                           >  1:1909028/1‑101 (MQ=255)
                                                            gggCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggg                                         <  1:4246974/101‑1 (MQ=255)
                                                              gCAAGAAGGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                       >  2:1158809/1‑101 (MQ=255)
                                                                 agaggGGGCCTCTCCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                    <  1:2338540/101‑1 (MQ=37)
                                                                    agGGGGCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                 <  2:1526650/101‑1 (MQ=255)
                                                                     gggggCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATgg                                <  1:513595/101‑1 (MQ=255)
                                                                         gCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                            <  2:3436257/101‑1 (MQ=255)
                                                                         gCTCTGCCC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                            <  1:1006474/101‑1 (MQ=255)
                                                                            ctgccc.ccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                         >  1:258922/5‑101 (MQ=255)
                                                                            ctaccc.ccGTCCCGTGCCGGGGCT.CCCCCGGGATATTTGATGCAAGATGAA...GGGGGGGTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                         <  1:51823/97‑1 (MQ=37)
                                                                               ccc.ccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCaga                      <  1:4090986/100‑1 (MQ=255)
                                                                                 c.ccggcccgtGCCGGGACT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGGGGGTTTCATCTTGCCGAAAATATCGCGGGGGGTGAATGGCGCAGCCAGAgg                    <  1:2938630/101‑1 (MQ=38)
                                                                                   ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggg                   >  2:929917/1‑101 (MQ=255)
                                                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGGTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                >  1:3163885/1‑101 (MQ=255)
                                                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                >  1:320558/1‑101 (MQ=255)
                                                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                >  2:2576331/1‑101 (MQ=255)
                                                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc               >  1:2453951/1‑101 (MQ=255)
                                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              <  1:3924492/101‑1 (MQ=255)
                                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              <  2:4291910/101‑1 (MQ=255)
                                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             <  2:1010498/101‑1 (MQ=255)
                                                                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                 >  2:1957836/8‑101 (MQ=21)
                                                                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                 <  1:1337573/94‑1 (MQ=18)
                                                                                          gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAATGGCGAAGCCAGAgggggg                >  2:744283/7‑101 (MQ=18)
                                                                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcgc          >  2:2978001/1‑101 (MQ=255)
                                                                                             ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcc         <  1:499750/101‑1 (MQ=255)
                                                                                               gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccc       >  2:110774/1‑101 (MQ=255)
                                                                                                  gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccct    <  2:2654579/100‑3 (MQ=255)
                                                                                                  aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccct    <  1:2906216/101‑3 (MQ=255)
                                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccccttc  >  1:1825755/1‑97 (MQ=255)
                                                                                                     |                                                                                                       
CATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CC.CCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249202‑2249400

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG