Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,374 +G 2.6% intergenic (‑170/‑135) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3742.→G 2.6% 6.4 0.0 ‑0.3152intergenic (‑170/‑135)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (81/67):  total (83/69)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.56e-01

Only 100 of 168 total aligned reads displayed.

GCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGC  >  NC_014034/2249275‑2249473
                                                                                                            |                                                                                                       
gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                                          <  1:1006474/101‑1 (MQ=255)
gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                                          <  2:3436257/101‑1 (MQ=255)
   ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                                     <  2:452480/97‑1 (MQ=255)
   ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                                     >  1:258922/5‑101 (MQ=255)
   ctacccccGTCCCGTGCCGGGGCT.CCCCCGGGATATTTGATGCAAGATGAA...GGGG..GGGTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                                     <  1:51823/97‑1 (MQ=37)
        cccggcccgtGCCGGGACT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGG..GGGTTTCATCTTGCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                                                <  1:2938630/101‑1 (MQ=38)
         ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                               >  2:929917/1‑101 (MQ=255)
            tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGGTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                            >  1:3163885/1‑101 (MQ=255)
             cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                           >  1:2453951/1‑101 (MQ=255)
             cccggGCCGGGCCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                           <  1:1570400/101‑1 (MQ=255)
              ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                                          <  2:4291910/101‑1 (MQ=255)
               cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                                             >  2:1957836/8‑101 (MQ=21)
               cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                                             <  1:1337573/94‑1 (MQ=18)
               cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                                             <  2:3398244/94‑1 (MQ=18)
                 caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                           <  1:2327243/96‑1 (MQ=18)
                 caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                           <  1:2900124/96‑1 (MQ=18)
                 caagcggACTCCCCCCGGGATATTGGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                           <  1:3882924/96‑1 (MQ=18)
                  gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g.c                                                                                   >  2:2978001/1‑101 (MQ=255)
                    cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc                                                                                 >  1:515202/1‑101 (MQ=255)
                     gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                >  1:421189/1‑101 (MQ=255)
                     cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c.g                                                                                     <  2:2752959/100‑1 (MQ=18)
                      ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccc                                                                               >  1:4337870/1‑101 (MQ=255)
                       gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccc                                                                              <  2:2566420/101‑2 (MQ=255)
                        gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                             <  2:2654579/100‑3 (MQ=255)
                        actccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.C.G.ccc                                                                                 <  1:2070380/97‑1 (MQ=17)
                        aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                             >  1:3225093/1‑99 (MQ=255)
                         cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccctt                                                                            >  2:1921007/1‑98 (MQ=255)
                          t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                           >  1:194212/1‑97 (MQ=255)
                          t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                           >  1:1825755/1‑97 (MQ=255)
                            cccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCt                                                                              <  1:3122854/101‑1 (MQ=0)
                             ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCtg                                                                             >  2:670123/1‑100 (MQ=2)
                             ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTcgc                                                                          <  2:1700602/101‑1 (MQ=255)
                              cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTcgcg                                                                         >  1:4389585/1‑101 (MQ=255)
                               ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgg                                                                        >  2:863530/1‑101 (MQ=255)
                                cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCtgccc                                                                          >  2:2044605/1‑97 (MQ=2)
                                cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcggg                                                                       >  2:1215378/1‑101 (MQ=255)
                                 gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                                                      >  1:954626/1‑101 (MQ=255)
                                 gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                                                      >  2:2718678/1‑101 (MQ=255)
                                  ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggcg                                                                     >  1:3601854/1‑101 (MQ=255)
                                    atgtttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGggcggc                                                                   <  2:1725741/98‑1 (MQ=255)
                                      atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCcg                                                                 <  1:1026188/101‑1 (MQ=255)
                                       tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCcgc                                                                >  1:814224/1‑101 (MQ=255)
                                        ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGTAG.C.GCCCCCCTCCGCGCGCGGCcgcc                                                               >  2:1098390/1‑100 (MQ=25)
                                          gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAc                                                             <  2:1451519/101‑1 (MQ=37)
                                            ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgc                                                           <  2:144875/101‑1 (MQ=255)
                                              cAAGATGAA...GGTG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgc                                                         <  2:488969/101‑1 (MQ=25)
                                               aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGCGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                        >  2:671280/1‑101 (MQ=255)
                                               aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                        >  2:434029/1‑101 (MQ=255)
                                               aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGA.GGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACgcgcgc                                                       >  1:3910052/1‑101 (MQ=255)
                                                  aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcggcg                                                     >  2:539962/1‑101 (MQ=255)
                                                  aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGAGcgcct                                                     >  2:4391130/1‑100 (MQ=255)
                                                  aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                     >  1:4210006/1‑101 (MQ=255)
                                                   tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                                    >  2:4191414/1‑101 (MQ=255)
                                                    gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CAC.CCCCCTTCGCGGGCGACCGCGACGCGCGCCGCa                                                   >  2:4016059/1‑101 (MQ=37)
                                                    gAA...GGGG..GGCTTTCATATTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                   >  2:485481/1‑101 (MQ=255)
                                                     aggaggggg..gcCTTTCATCTGGCCGAAAATTTCCGGGGGGGGGAA.TGGCGCA..GCCAGGGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                     <  1:1626833/96‑1 (MQ=255)
                                                      a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                 >  1:1524429/1‑101 (MQ=255)
                                                      a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                 <  2:588497/101‑1 (MQ=255)
                                                      a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                 <  2:2410089/101‑1 (MQ=255)
                                                      a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                 >  2:1113979/1‑101 (MQ=255)
                                                      a...GGGG..GCCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                 <  2:4321548/101‑1 (MQ=255)
                                                                ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                            <  2:4231711/101‑1 (MQ=255)
                                                                ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.CGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                            <  2:110667/101‑1 (MQ=255)
                                                                   tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                         >  2:3201805/1‑101 (MQ=255)
                                                                   tttCATCTTGCCAAAAATGGCCGGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                         <  1:956221/101‑1 (MQ=37)
                                                                    ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCGGCAACAGGGCGTGc                                        >  2:1858893/1‑101 (MQ=25)
                                                                      cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                      <  2:180444/101‑1 (MQ=255)
                                                                       aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                     >  1:991653/1‑101 (MQ=255)
                                                                         cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                   <  2:239874/101‑1 (MQ=255)
                                                                          ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                  <  1:1921131/101‑1 (MQ=255)
                                                                              cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                              <  2:4034098/101‑1 (MQ=255)
                                                                               gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                             >  2:2391509/1‑101 (MQ=255)
                                                                                aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                            >  2:3879374/1‑101 (MQ=255)
                                                                                aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                            >  2:536435/1‑101 (MQ=255)
                                                                                 aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCCTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTAGTGCCCCTTTGTGATTc                           >  2:2108685/1‑101 (MQ=37)
                                                                                  aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                          <  2:258895/101‑1 (MQ=255)
                                                                                   atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCCG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGAACGTGCCCCTTTGTCATTCTg                         >  2:611320/1‑101 (MQ=37)
                                                                                    tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCTGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                        >  1:492938/1‑101 (MQ=255)
                                                                                    tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                        >  1:927189/1‑101 (MQ=255)
                                                                                    tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                        <  2:3377513/101‑1 (MQ=255)
                                                                                     atCCCGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                       <  2:3529664/101‑1 (MQ=255)
                                                                                     agccgggggggTGAA.GGGCGCA..GCCAGGGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                       <  1:79765/96‑1 (MQ=255)
                                                                                       ccgggggggTGAA.TGGAGCA..CCCGGAGGGGGGCGG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                     <  2:2243063/98‑1 (MQ=255)
                                                                                         cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                   <  1:3488849/101‑1 (MQ=255)
                                                                                           gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                 >  1:2787214/1‑101 (MQ=255)
                                                                                           gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                 >  2:2220648/1‑101 (MQ=255)
                                                                                           gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                 >  1:3729780/1‑101 (MQ=255)
                                                                                             gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt               <  1:480133/101‑1 (MQ=255)
                                                                                             gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt               <  1:1240832/101‑1 (MQ=255)
                                                                                               gTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt             <  1:241747/101‑1 (MQ=255)
                                                                                                  aa.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt          >  1:3500343/1‑101 (MQ=255)
                                                                                                   a.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGACGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa         >  2:2782907/1‑101 (MQ=255)
                                                                                                     tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa        >  2:3111793/1‑101 (MQ=255)
                                                                                                     tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa        >  1:4076855/1‑101 (MQ=255)
                                                                                                       gcgcA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGc      <  2:2013701/101‑1 (MQ=255)
                                                                                                        cgcA..GCCAGGGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt     <  1:594135/101‑1 (MQ=255)
                                                                                                         gcA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg    >  1:3055464/1‑101 (MQ=255)
                                                                                                          cA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg   >  1:2748055/1‑101 (MQ=255)
                                                                                                          cA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGCCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg   <  1:295614/101‑1 (MQ=255)
                                                                                                           a..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc  >  2:1525885/1‑101 (MQ=255)
                                                                                                            |                                                                                                       
GCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGC  >  NC_014034/2249275‑2249473

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG