Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,389 +C 2.7% intergenic (‑185/‑120) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3891.→C 2.7% 4.7 ‑1.2 ‑0.0150intergenic (‑185/‑120)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (1/3):  ref base (85/61):  total (86/64)
Fisher's exact test strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 166 total aligned reads displayed.

CGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCC  >  NC_014034/2249289‑2249486
                                                                                                              |                                                                                                   
   gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g.c                                                                                                >  2:2978001/1‑101 (MQ=255)
     cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc                                                                                              >  1:515202/1‑101 (MQ=255)
      gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                             >  2:630121/1‑101 (MQ=255)
      gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                             >  2:110774/1‑101 (MQ=255)
      gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                             >  2:508026/1‑101 (MQ=255)
      gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                             >  1:421189/1‑101 (MQ=255)
      cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c.g                                                                                                  <  2:2752959/100‑1 (MQ=18)
       ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccc                                                                                            >  1:4337870/1‑101 (MQ=255)
        gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccc                                                                                           <  2:2566420/101‑2 (MQ=255)
         gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                                          <  2:2654579/100‑3 (MQ=255)
         actccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.C.G.ccc                                                                                              <  1:2070380/97‑1 (MQ=17)
         aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                                          <  2:3414293/101‑3 (MQ=255)
         aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                                          <  1:2906216/101‑3 (MQ=255)
         aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                                          >  1:3225093/1‑99 (MQ=255)
          cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccctt                                                                                         >  2:1921007/1‑98 (MQ=255)
          actacgccGGGATATTTGGGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATCTCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.T.cccccct                                                                                          <  2:1125168/95‑3 (MQ=25)
           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                                        >  1:1825755/1‑97 (MQ=255)
           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                                        >  2:594067/1‑97 (MQ=255)
             cccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCt                                                                                           <  1:3122854/101‑1 (MQ=0)
             cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttcg                                                                                       <  1:591924/101‑6 (MQ=255)
               cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTcgcg                                                                                      >  1:4389585/1‑101 (MQ=255)
                ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgg                                                                                     >  1:1603494/1‑101 (MQ=255)
                ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgg                                                                                     >  2:863530/1‑101 (MQ=255)
                  gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                                                                   >  1:3009782/1‑101 (MQ=255)
                  gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                                                                   >  1:954626/1‑101 (MQ=255)
                  gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                                                                   >  2:2718678/1‑101 (MQ=255)
                   ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggcg                                                                                  >  1:3601854/1‑101 (MQ=255)
                     atgtttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGggcggc                                                                                <  2:1725741/98‑1 (MQ=255)
                       atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCcg                                                                              <  1:1026188/101‑1 (MQ=255)
                        tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCcgc                                                                             >  1:814224/1‑101 (MQ=255)
                         ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGTAG.C.GCCCCCCTCCGCGCGCGGCcgcc                                                                            >  2:1098390/1‑100 (MQ=25)
                           gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAc                                                                          <  2:1451519/101‑1 (MQ=37)
                             ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgc                                                                        <  2:144875/101‑1 (MQ=255)
                               cAAGATGAA...GGTG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgc                                                                      <  2:488969/101‑1 (MQ=25)
                               cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgc                                                                      >  2:2892422/1‑101 (MQ=255)
                                aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGCGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                                     >  2:671280/1‑101 (MQ=255)
                                aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                                     >  2:434029/1‑101 (MQ=255)
                                aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                                     >  2:1578778/1‑101 (MQ=255)
                                aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGA.GGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACgcgcgc                                                                    >  1:3910052/1‑101 (MQ=255)
                                  gaggAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                                   <  2:1825631/98‑1 (MQ=255)
                                   aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGTCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                                  <  2:421738/101‑1 (MQ=255)
                                   aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                                  <  1:2627658/101‑1 (MQ=255)
                                   aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGAGcgcct                                                                  >  2:4391130/1‑100 (MQ=255)
                                   aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                                  >  1:4210006/1‑101 (MQ=255)
                                    tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                                                 >  2:4191414/1‑101 (MQ=255)
                                     gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CAC.CCCCCTTCGCGGGCGACCGCGACGCGCGCCGCa                                                                >  2:4016059/1‑101 (MQ=37)
                                      aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                                               <  2:1254047/101‑1 (MQ=255)
                                       a...GGGG..GGCTTTCATCTTGCGGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                              <  2:1976457/101‑1 (MQ=255)
                                       a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                              >  1:1524429/1‑101 (MQ=255)
                                       a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                              <  2:588497/101‑1 (MQ=255)
                                       a...GGGG..GCCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                              <  2:4321548/101‑1 (MQ=255)
                                            ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                            <  1:3468796/101‑1 (MQ=255)
                                            ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                            >  1:1937042/1‑101 (MQ=255)
                                                 ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                         <  2:4231711/101‑1 (MQ=255)
                                                 ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                         >  1:1295710/1‑101 (MQ=255)
                                                 ggCTTTCATCTTGCCAAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                         <  1:83749/101‑1 (MQ=38)
                                                  ggtttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                                                        <  2:2138991/99‑1 (MQ=255)
                                                    tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                      >  2:3201805/1‑101 (MQ=255)
                                                    tttCATCTTGCCAAAAATGGCCGGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                      <  1:956221/101‑1 (MQ=37)
                                                     ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCGGCAACAGGGCGTGc                                                     >  2:1858893/1‑101 (MQ=25)
                                                        aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                  >  1:991653/1‑101 (MQ=255)
                                                          tttGCCGAAAATATCCCGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCGG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                <  1:2463749/100‑1 (MQ=37)
                                                          cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                <  2:239874/101‑1 (MQ=255)
                                                           ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                               <  1:1921131/101‑1 (MQ=255)
                                                           ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                               >  2:477158/1‑101 (MQ=255)
                                                            tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                              >  2:231073/1‑101 (MQ=255)
                                                               cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                           <  2:4034098/101‑1 (MQ=255)
                                                                gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                          >  2:2391509/1‑101 (MQ=255)
                                                                 aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                         >  2:3879374/1‑101 (MQ=255)
                                                                  aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                        >  1:754383/1‑101 (MQ=255)
                                                                  aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCCTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTAGTGCCCCTTTGTGATTc                                        >  2:2108685/1‑101 (MQ=37)
                                                                   aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                       <  2:258895/101‑1 (MQ=255)
                                                                     tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCTGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                                     >  1:492938/1‑101 (MQ=255)
                                                                     tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                                     <  2:3377513/101‑1 (MQ=255)
                                                                     tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                                     >  1:927189/1‑101 (MQ=255)
                                                                      atCCCGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                                    <  2:3529664/101‑1 (MQ=255)
                                                                          cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                                <  1:3488849/101‑1 (MQ=255)
                                                                            gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                              >  1:3729780/1‑101 (MQ=255)
                                                                            gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                              >  1:2787214/1‑101 (MQ=255)
                                                                            gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                              >  2:2220648/1‑101 (MQ=255)
                                                                             ggggTGAA.GGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGCCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                             <  1:3740365/101‑1 (MQ=255)
                                                                              gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                            <  1:1240832/101‑1 (MQ=255)
                                                                              gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                            <  1:480133/101‑1 (MQ=255)
                                                                                gTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                          <  1:241747/101‑1 (MQ=255)
                                                                                    a.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGACGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                      >  2:2782907/1‑101 (MQ=255)
                                                                                      tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                     >  2:3111793/1‑101 (MQ=255)
                                                                                      tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                     >  2:539944/1‑101 (MQ=255)
                                                                                        gcgcA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGc                   <  2:2013701/101‑1 (MQ=255)
                                                                                         cgcA..TCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                  >  1:3491008/1‑101 (MQ=255)
                                                                                          gcA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg                 >  1:3055464/1‑101 (MQ=255)
                                                                                           cA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg                >  1:2748055/1‑101 (MQ=255)
                                                                                            a..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc               >  2:1525885/1‑101 (MQ=255)
                                                                                                 cAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc            >  1:1611574/1‑101 (MQ=255)
                                                                                                  agagGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACCCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCgaa           >  2:1062170/1‑99 (MQ=255)
                                                                                                   gagGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt          <  1:1084318/101‑1 (MQ=255)
                                                                                                      gggggCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGa       >  2:20465/1‑101 (MQ=255)
                                                                                                       ggggCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGAt      >  2:3251798/1‑101 (MQ=255)
                                                                                                         ggCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGt    <  2:907678/101‑1 (MQ=255)
                                                                                                           cAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTcc  >  1:2979382/1‑101 (MQ=255)
                                                                                                           cAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTcc  >  2:1115044/1‑101 (MQ=255)
                                                                                                              |                                                                                                   
CGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCC  >  NC_014034/2249289‑2249486

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG