Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,391 +C 94.7% intergenic (‑187/‑118) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3911.→C94.7% 15.2 ‑0.8 ‑0.0133intergenic (‑187/‑118)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (73/53):  ref base (3/4):  total (76/57)
Fisher's exact test strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.20e-01

Only 100 of 166 total aligned reads displayed.

GCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCCGAAC  >  NC_014034/2249292‑2249490
                                                                                                               |                                                                                                   
gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g.c                                                                                                    >  2:2978001/1‑101 (MQ=255)
  cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc                                                                                                  >  1:515202/1‑101 (MQ=255)
   gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                                 >  1:421189/1‑101 (MQ=255)
   gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                                 >  2:630121/1‑101 (MQ=255)
   gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                                                                 >  2:508026/1‑101 (MQ=255)
   cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c.g                                                                                                      <  2:2752959/100‑1 (MQ=18)
    ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccc                                                                                                >  1:4337870/1‑101 (MQ=255)
      actccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.C.G.ccc                                                                                                  <  1:2070380/97‑1 (MQ=17)
      aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                                              >  1:3225093/1‑99 (MQ=255)
      aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                                              <  1:2906216/101‑3 (MQ=255)
      aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                                                              <  2:3414293/101‑3 (MQ=255)
       actacgccGGGATATTTGGGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATCTCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.T.cccccct                                                                                              <  2:1125168/95‑3 (MQ=25)
        t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                                            >  1:1825755/1‑97 (MQ=255)
        t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                                                            >  2:594067/1‑97 (MQ=255)
         ccccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.ccccc                                                                                                <  2:2095384/100‑1 (MQ=0)
          cccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCt                                                                                               <  1:3122854/101‑1 (MQ=0)
          cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttcg                                                                                           <  1:591924/101‑6 (MQ=255)
           ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTcgc                                                                                           <  2:1700602/101‑1 (MQ=255)
            cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTcgcg                                                                                          >  1:4389585/1‑101 (MQ=255)
             ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgg                                                                                         >  1:1603494/1‑101 (MQ=255)
             ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgg                                                                                         >  2:863530/1‑101 (MQ=255)
              cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCtgccc                                                                                           >  2:2044605/1‑97 (MQ=2)
              cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcggg                                                                                        >  2:1215378/1‑101 (MQ=255)
               gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                                                                       >  1:954626/1‑101 (MQ=255)
               gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                                                                       >  1:3009782/1‑101 (MQ=255)
                ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggcg                                                                                      >  1:3601854/1‑101 (MQ=255)
                    atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCcg                                                                                  <  1:1026188/101‑1 (MQ=255)
                     tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCcgc                                                                                 >  1:814224/1‑101 (MQ=255)
                      ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGTAG.C.GCCCCCCTCCGCGCGCGGCcgcc                                                                                >  2:1098390/1‑100 (MQ=25)
                        gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAc                                                                              <  2:1451519/101‑1 (MQ=37)
                            cAAGATGAA...GGTG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgc                                                                          <  2:488969/101‑1 (MQ=25)
                             aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGCGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                                         >  2:671280/1‑101 (MQ=255)
                             aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                                         >  2:434029/1‑101 (MQ=255)
                             aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                                         >  2:1578778/1‑101 (MQ=255)
                             aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                                                                         >  2:1480789/1‑101 (MQ=255)
                             aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGA.GGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACgcgcgc                                                                        >  1:3910052/1‑101 (MQ=255)
                                aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGTCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                                      <  2:421738/101‑1 (MQ=255)
                                aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                                      <  1:2627658/101‑1 (MQ=255)
                                aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGAGcgcct                                                                      >  2:4391130/1‑100 (MQ=255)
                                aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                                      >  1:4210006/1‑101 (MQ=255)
                                 tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                                                     >  2:4191414/1‑101 (MQ=255)
                                 tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                                                     >  2:2393415/1‑101 (MQ=255)
                                  gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CAC.CCCCCTTCGCGGGCGACCGCGACGCGCGCCGCa                                                                    >  2:4016059/1‑101 (MQ=37)
                                    a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                                  >  1:1524429/1‑101 (MQ=255)
                                    a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                                  >  2:1113979/1‑101 (MQ=255)
                                    a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                                  <  2:2410089/101‑1 (MQ=255)
                                    a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                                  <  2:588497/101‑1 (MQ=255)
                                    a...GGGG..GCCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                                  <  2:4321548/101‑1 (MQ=255)
                                         ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                                >  1:1937042/1‑101 (MQ=255)
                                         ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                                <  1:3468796/101‑1 (MQ=255)
                                              ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                             <  2:4231711/101‑1 (MQ=255)
                                              ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                             >  1:1295710/1‑101 (MQ=255)
                                              ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.CGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                             <  2:110667/101‑1 (MQ=255)
                                              ggCTTTCATCTTGCCAAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                             <  1:83749/101‑1 (MQ=38)
                                               ggtttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                                                            <  2:2138991/99‑1 (MQ=255)
                                                 tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                          >  2:3201805/1‑101 (MQ=255)
                                                 tttCATCTTGCCAAAAATGGCCGGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                          <  1:956221/101‑1 (MQ=37)
                                                  ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCGGCAACAGGGCGTGc                                                         >  2:1858893/1‑101 (MQ=25)
                                                    cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                                       <  2:180444/101‑1 (MQ=255)
                                                     aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                      >  1:991653/1‑101 (MQ=255)
                                                       tttGCCGAAAATATCCCGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCGG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                    <  1:2463749/100‑1 (MQ=37)
                                                        ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                                   >  2:477158/1‑101 (MQ=255)
                                                        ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                                   <  1:1921131/101‑1 (MQ=255)
                                                            cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                               <  2:4034098/101‑1 (MQ=255)
                                                             gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                              >  2:2391509/1‑101 (MQ=255)
                                                              aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                             >  2:3879374/1‑101 (MQ=255)
                                                               aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                            >  1:754383/1‑101 (MQ=255)
                                                                aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                           <  2:258895/101‑1 (MQ=255)
                                                                  tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCTGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                                         >  1:492938/1‑101 (MQ=255)
                                                                  tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                                         <  2:3377513/101‑1 (MQ=255)
                                                                  tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                                         >  1:927189/1‑101 (MQ=255)
                                                                   atCCCGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                                        <  2:3529664/101‑1 (MQ=255)
                                                                     ccgggggggTGAA.TGGAGCA..CCCGGAGGGGGGCGG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                                      <  2:2243063/98‑1 (MQ=255)
                                                                       cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                                    <  1:3488849/101‑1 (MQ=255)
                                                                         gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                                  >  1:3729780/1‑101 (MQ=255)
                                                                         gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                                  >  1:2787214/1‑101 (MQ=255)
                                                                          ggggTGAA.GGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGCCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                                 <  1:3740365/101‑1 (MQ=255)
                                                                           gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                                <  1:480133/101‑1 (MQ=255)
                                                                           gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                                <  1:1240832/101‑1 (MQ=255)
                                                                             gTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                              <  1:241747/101‑1 (MQ=255)
                                                                              tGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                             <  2:194185/101‑1 (MQ=255)
                                                                                   tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                         >  2:3111793/1‑101 (MQ=255)
                                                                                   tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                         >  2:539944/1‑101 (MQ=255)
                                                                                    ggCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg                        <  2:2715020/101‑1 (MQ=255)
                                                                                     gcgcA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGc                       <  2:2013701/101‑1 (MQ=255)
                                                                                      cgcA..TCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                      >  1:3491008/1‑101 (MQ=255)
                                                                                       gcA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg                     >  1:3055464/1‑101 (MQ=255)
                                                                                        cA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg                    >  1:2748055/1‑101 (MQ=255)
                                                                                              cggagGGGGGCAC.C.GCCCCCCTCCGGGGGCGGCCGCGACGCGCGCCGCAACACGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc                <  2:276194/99‑1 (MQ=255)
                                                                                              cAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc                >  1:1611574/1‑101 (MQ=255)
                                                                                              cAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc                >  2:191326/1‑101 (MQ=255)
                                                                                               agagGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa               >  2:14323/1‑101 (MQ=255)
                                                                                                gagGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt              >  2:2605018/1‑101 (MQ=255)
                                                                                                gagGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt              <  2:2175532/101‑1 (MQ=255)
                                                                                                gagGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt              <  1:1084318/101‑1 (MQ=255)
                                                                                                    ggggCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGAt          >  2:3251798/1‑101 (MQ=255)
                                                                                                      ggCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGt        <  2:907678/101‑1 (MQ=255)
                                                                                                       gCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc       >  2:2793965/1‑101 (MQ=255)
                                                                                                        cAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTcc      >  1:2979382/1‑101 (MQ=255)
                                                                                                             tg.cCCCGTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCCGAAc  <  2:3009561/100‑1 (MQ=255)
                                                                                                               |                                                                                                   
GCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCCGAAC  >  NC_014034/2249292‑2249490

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG