Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,896 +C 100% coding (2679/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,8961.→C100.0% 594.8 171coding (2679/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (71/98):  ref base (1/1):  total (72/99)

Only 100 of 205 total aligned reads displayed.

GGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304797‑2304988
                                                                                                     |                                                                                             
ggCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.c                                                                                              >  2:1144995/1‑101 (MQ=255)
 gCAAGATCCTGATAATCTTGCGCAAATCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.cc                                                                                             <  1:2694007/101‑2 (MQ=255)
 gCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.cc                                                                                             >  2:1820866/1‑100 (MQ=255)
  cAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccg                                                                                            >  2:1481372/1‑99 (MQ=255)
  cAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCGGTCAGCCGGCCTGCCGCAAGGGAGGCGACGGTGGCG.CCTGCATGCGGAAGACcg.ccg                                                                                            >  1:3809376/1‑99 (MQ=255)
   aaGATCCTTATAATCTTGCCCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                           <  2:3693107/101‑4 (MQ=255)
   aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                           >  1:344765/1‑98 (MQ=255)
   aaGATCCTGATAATCTTGCACAAACCTCGGCGGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                           <  2:245247/101‑4 (MQ=255)
    aGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtc                                                                                          <  1:2847275/101‑5 (MQ=255)
    aGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtc                                                                                          <  1:831695/101‑5 (MQ=255)
      aTCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcag                                                                                        >  1:298682/1‑95 (MQ=255)
      aTCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcag                                                                                        >  2:2729840/1‑95 (MQ=255)
       tCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcagc                                                                                       <  1:658498/101‑8 (MQ=255)
          tGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcagctcc                                                                                    >  1:3473698/1‑91 (MQ=255)
             tAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGc                                                                                  >  1:3205626/1‑101 (MQ=255)
              aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCGTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCa                                                                                 >  2:2276797/1‑101 (MQ=255)
               aTCTTGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                               <  1:1549174/101‑1 (MQ=255)
               aTCTTGCGCAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                               <  1:3277092/101‑1 (MQ=255)
                tCTTGCGCAAACCCGGGCCGAGTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gg                                                                              <  2:4325987/101‑1 (MQ=255)
                  ttGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggt                                                                            <  2:1224791/101‑3 (MQ=255)
                  ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggt                                                                            >  2:3904413/1‑99 (MQ=255)
                    gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          <  2:1332496/101‑5 (MQ=255)
                    gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          <  2:3337608/101‑5 (MQ=255)
                    gcgcAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          <  1:4022228/101‑5 (MQ=255)
                    gcgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          >  2:1799642/1‑97 (MQ=255)
                        aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcgc                                                                       >  1:3915596/1‑101 (MQ=255)
                        aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcgc                                                                       >  2:3935996/1‑101 (MQ=255)
                          accTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                     <  1:2506183/99‑1 (MQ=255)
                          aaCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                     <  1:1250503/101‑1 (MQ=255)
                            cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                   >  2:2646087/1‑101 (MQ=255)
                              cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGcc                                                                 >  2:4369382/1‑101 (MQ=255)
                              cGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCgac                                                                 <  2:2366327/101‑3 (MQ=255)
                              cGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGcc                                                                 <  1:1820985/101‑1 (MQ=255)
                               ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                >  2:2320140/1‑101 (MQ=255)
                                gCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                               <  2:1927523/101‑1 (MQ=255)
                                gCCGGACCCGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                               <  2:1285012/101‑1 (MQ=255)
                                  cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                             <  1:3389620/101‑1 (MQ=255)
                                    gtgccGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                           <  1:460213/98‑1 (MQ=255)
                                     aTCGGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                          <  1:2962098/101‑1 (MQ=255)
                                        cgcccTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                       <  1:1079309/98‑1 (MQ=255)
                                        cGGCGTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                       <  1:1457026/101‑1 (MQ=255)
                                         ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      >  1:3044714/1‑101 (MQ=255)
                                         ggCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      <  2:3651209/101‑1 (MQ=255)
                                         ggCCTGCCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      <  1:3723407/101‑1 (MQ=255)
                                            cTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                   >  1:688750/1‑101 (MQ=255)
                                                cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               <  2:2833590/101‑1 (MQ=255)
                                                cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               >  1:758588/1‑101 (MQ=255)
                                                cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGCcgc                                               >  1:1102816/1‑101 (MQ=255)
                                                cAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               <  2:3548080/101‑1 (MQ=255)
                                                 cgCCGCCCTGCCGCAACGGCGGCGCCGGTGGC.CCCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                              <  2:1262834/100‑1 (MQ=255)
                                                   ccGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                            <  2:2178498/101‑1 (MQ=255)
                                                     ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                          >  1:3542200/1‑101 (MQ=255)
                                                       ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                        <  1:3785492/101‑1 (MQ=255)
                                                         tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcggt                                      >  1:911119/1‑99 (MQ=255)
                                                          gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     <  1:2320290/101‑1 (MQ=255)
                                                           ccGCAACGGCGGCGCCGGTGGCG.CCTGCATTCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                    <  1:1777363/101‑1 (MQ=255)
                                                           ccGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                    <  1:1660268/101‑1 (MQ=255)
                                                            cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                   >  2:1406772/1‑101 (MQ=255)
                                                             gCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                  <  1:2466094/101‑1 (MQ=255)
                                                              cAACGGGGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 <  2:1867057/101‑1 (MQ=255)
                                                              cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 >  1:2182722/1‑101 (MQ=255)
                                                              cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCAGCCGCGTTTTCCt                                 <  1:3860228/101‑1 (MQ=255)
                                                               aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:2752991/101‑1 (MQ=255)
                                                               aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGGTTTCCTc                                >  1:4253484/1‑101 (MQ=255)
                                                                  ggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                             <  2:1668840/101‑1 (MQ=255)
                                                                   gcggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  1:3122769/101‑1 (MQ=255)
                                                                     ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  2:3318718/101‑1 (MQ=255)
                                                                     ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          >  1:2207655/1‑101 (MQ=255)
                                                                     ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          >  2:4137313/1‑101 (MQ=255)
                                                                     ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  2:4294936/101‑1 (MQ=255)
                                                                       cgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  2:382375/101‑1 (MQ=25)
                                                                          cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     <  2:754918/101‑1 (MQ=25)
                                                                           ggTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  1:2710671/1‑101 (MQ=25)
                                                                           ggTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    <  1:750815/101‑1 (MQ=25)
                                                                           ggTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  1:1857505/1‑101 (MQ=25)
                                                                               ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  1:3672058/97‑1 (MQ=25)
                                                                                gcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                <  2:2754145/98‑1 (MQ=25)
                                                                                 cgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               <  2:2808642/99‑1 (MQ=25)
                                                                                 cgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               >  2:3448228/3‑101 (MQ=25)
                                                                                  gccTGCATGCGGAATACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTAGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              <  2:189108/100‑1 (MQ=21)
                                                                                  gccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              >  1:3329215/2‑101 (MQ=21)
                                                                                    cTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt            <  1:4369653/101‑1 (MQ=25)
                                                                                     tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           <  1:59391/101‑1 (MQ=25)
                                                                                     tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           >  1:1508958/1‑101 (MQ=21)
                                                                                     tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           >  1:1969123/1‑101 (MQ=21)
                                                                                     tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           <  2:3162609/101‑1 (MQ=25)
                                                                                     tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           <  2:298647/101‑1 (MQ=25)
                                                                                     tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           >  2:2207574/1‑101 (MQ=21)
                                                                                      gCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          <  1:1989373/101‑1 (MQ=25)
                                                                                      gCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          >  1:808838/1‑101 (MQ=21)
                                                                                      gCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          <  2:420596/101‑1 (MQ=25)
                                                                                        atgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt        <  1:4137573/96‑1 (MQ=25)
                                                                                        atgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt        <  1:4300742/96‑1 (MQ=25)
                                                                                        atgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt        >  2:4224339/6‑101 (MQ=21)
                                                                                        atgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCCGGGt        >  2:2017423/6‑101 (MQ=21)
                                                                                         tgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg       <  2:3886789/97‑1 (MQ=25)
                                                                                           cggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCTGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc     <  1:2121578/99‑1 (MQ=21)
                                                                                             gAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   >  2:2865112/1‑101 (MQ=21)
                                                                                             gAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   >  2:620207/1‑101 (MQ=21)
                                                                                              aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  >  1:2911795/1‑101 (MQ=21)
                                                                                                     |                                                                                             
GGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304797‑2304988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG