Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,910 +G 100% coding (2665/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9101.→G100.0% 616.3 180coding (2665/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (81/97):  ref base (1/0):  total (82/98)

Only 100 of 201 total aligned reads displayed.

AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACC.G.C.GTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005
                                                                                                        |                                                                                               
aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCa                                                                                                  >  2:4141316/1‑101 (MQ=255)
 aTCTTGCGCAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCA.g                                                                                                <  1:3277092/101‑1 (MQ=255)
  tCTTGCGCAAACCCGGGCCGAGTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCA.gg                                                                                               <  2:4325987/101‑1 (MQ=255)
  tCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCTGCTGGCG.CCTGCATGCGGGAGACC.GCC.GTCAGCTCCCGCA.gg                                                                                               >  2:1769368/1‑101 (MQ=255)
    ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCA.gggt                                                                                             >  2:3904413/1‑99 (MQ=255)
     tGCGAAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCA.gggtc                                                                                            <  2:3330584/101‑4 (MQ=255)
      gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCA.gggtct                                                                                           <  2:1332496/101‑5 (MQ=255)
      gcgcAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCA.gggtct                                                                                           <  1:4022228/101‑5 (MQ=255)
      gcgaAAACCCCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCA.gggtct                                                                                           <  1:3095394/101‑5 (MQ=255)
          aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTgcgc                                                                                        >  2:3935996/1‑101 (MQ=255)
          aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTgcgc                                                                                        >  1:3915596/1‑101 (MQ=255)
            aaCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCt                                                                                      <  1:1250503/101‑1 (MQ=255)
              cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                                    >  2:2646087/1‑101 (MQ=255)
              cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGGCTGCGCCTCg                                                                                    >  2:1235116/1‑101 (MQ=255)
               tCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCGGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGc                                                                                   >  1:3741908/1‑101 (MQ=255)
               ccGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGc                                                                                   <  2:2309173/100‑1 (MQ=255)
                cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGcc                                                                                  >  2:4369382/1‑101 (MQ=255)
                cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGcc                                                                                  >  1:3887043/1‑101 (MQ=255)
                 ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                                 <  2:3348117/101‑1 (MQ=255)
                 ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                                 >  1:851265/1‑101 (MQ=255)
                  gCCGGTGCCGCCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCATGGTCTGCGCCTCGCCca                                                                                <  1:4370636/101‑1 (MQ=255)
                  gCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                                                <  2:1927523/101‑1 (MQ=255)
                  gCCGGACCCGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                                                <  2:1285012/101‑1 (MQ=255)
                   ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCcag                                                                               >  1:345983/1‑101 (MQ=255)
                   ccGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCcag                                                                               <  2:2379206/101‑1 (MQ=255)
                    cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                                              <  1:3389620/101‑1 (MQ=255)
                      gtgccGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                                            <  1:460213/98‑1 (MQ=255)
                      gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                                            >  1:4149768/1‑101 (MQ=255)
                       aTCGGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                           <  1:2962098/101‑1 (MQ=255)
                         ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGCGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                                         >  1:2731578/1‑101 (MQ=255)
                          cgcccTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                                        <  1:1079309/98‑1 (MQ=255)
                          cGGCGTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                                        <  1:1457026/101‑1 (MQ=255)
                              cTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                                    >  1:688750/1‑101 (MQ=255)
                                  cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                                                >  1:758588/1‑101 (MQ=255)
                                  cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGCcgc                                                                >  1:1102816/1‑101 (MQ=255)
                                   cgCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                                               <  1:3948868/100‑1 (MQ=255)
                                    gCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCgg                                                              >  2:1809771/1‑101 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                           >  1:3542200/1‑101 (MQ=255)
                                         ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                                         <  2:22239/101‑1 (MQ=255)
                                         ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                                         <  1:3790176/101‑1 (MQ=255)
                                          cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGt                                                        >  1:2163115/1‑101 (MQ=255)
                                           tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                                       >  2:1961203/1‑101 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                                      <  1:2320290/101‑1 (MQ=255)
                                              cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                    >  2:1406772/1‑101 (MQ=255)
                                               gCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                                   >  1:4286349/1‑101 (MQ=255)
                                                cAACGGGGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                                  <  2:1867057/101‑1 (MQ=255)
                                                cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                                  >  1:2627771/1‑101 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCTC.C.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGGTTTCCTc                                                 >  2:390057/1‑101 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 <  1:1918672/101‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 <  1:2752991/101‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 >  2:1186997/1‑101 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCGGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 <  1:1519877/101‑1 (MQ=255)
                                                    ggcggcGCCGGTGGCG.CCTGCATGCGGAAGGCC.GCC.GTCAGCTGCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                              <  1:288152/101‑1 (MQ=255)
                                                    ggcggcGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                              <  2:1668840/101‑1 (MQ=255)
                                                     gcggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                                             <  2:2190246/101‑1 (MQ=255)
                                                     gcggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                                             <  2:3473458/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           <  2:4294936/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           >  1:2207655/1‑101 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           <  2:3318718/101‑1 (MQ=255)
                                                         cgcCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                                         <  2:382375/101‑1 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                      <  2:754918/101‑1 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                      <  1:2276942/101‑1 (MQ=25)
                                                             ggTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     >  1:4072745/1‑101 (MQ=25)
                                                              gTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                                    >  2:1452205/1‑101 (MQ=25)
                                                              gTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTCCGCCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                                    >  1:383511/1‑101 (MQ=25)
                                                               tGGCG.CCTGCATGCGGAAGGCC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   <  1:434519/101‑1 (MQ=25)
                                                               tGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   <  1:2496417/101‑1 (MQ=25)
                                                               tGGCG.CCTGCATGCGGAAGACC.G.CGGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   <  1:1834542/101‑1 (MQ=25)
                                                                 ggcgccTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  <  1:3672058/97‑1 (MQ=25)
                                                                  gcgccTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                 <  2:2754145/98‑1 (MQ=25)
                                                                   cgccTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                                <  2:2808642/99‑1 (MQ=25)
                                                                    gccTGCATGCGGAATACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTAGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg                               <  2:189108/100‑1 (MQ=21)
                                                                    gccTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg                               >  1:3329215/2‑101 (MQ=21)
                                                                      cTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt                             <  1:3026505/101‑1 (MQ=25)
                                                                       tGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg                            >  1:1969123/1‑101 (MQ=21)
                                                                       tGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg                            <  2:3162609/101‑1 (MQ=25)
                                                                       tGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg                            <  2:298647/101‑1 (MQ=25)
                                                                       tGCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg                            <  1:59391/101‑1 (MQ=25)
                                                                        gCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                           <  2:420596/101‑1 (MQ=25)
                                                                        gCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                           <  1:1989373/101‑1 (MQ=25)
                                                                        gCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                           >  1:808838/1‑101 (MQ=21)
                                                                        gCATGCGGAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                           <  1:1799761/101‑1 (MQ=25)
                                                                          atgcggAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt                         >  2:4224339/6‑101 (MQ=21)
                                                                          atgcggAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCCGGGt                         >  2:2017423/6‑101 (MQ=21)
                                                                               gAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt                    >  2:620207/1‑101 (MQ=21)
                                                                               gAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt                    >  2:2820692/1‑101 (MQ=21)
                                                                               gAAGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt                    >  2:2865112/1‑101 (MQ=21)
                                                                                aaGACC.GCC.GTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                   <  2:3593923/101‑1 (MQ=21)
                                                                                     gccgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATc                >  2:3989890/11‑101 (MQ=21)
                                                                                       cgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCaa              >  2:2337838/9‑101 (MQ=25)
                                                                                        gcggtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             >  1:2554253/8‑101 (MQ=25)
                                                                                        gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             <  2:3667288/94‑1 (MQ=21)
                                                                                            tcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTgg         >  1:371510/4‑101 (MQ=255)
                                                                                             cagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt        >  1:1649743/3‑101 (MQ=255)
                                                                                             cagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt        >  1:3138524/3‑101 (MQ=255)
                                                                                               gCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTc      >  2:660114/1‑101 (MQ=255)
                                                                                                 tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCaa    >  1:945657/1‑100 (MQ=255)
                                                                                                 tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    >  1:2049485/1‑101 (MQ=255)
                                                                                                 tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    >  1:1679585/1‑101 (MQ=255)
                                                                                                   ccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAccg  <  2:375571/101‑1 (MQ=255)
                                                                                                        |                                                                                               
AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACC.G.C.GTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG