Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,330,175 +C 72.2% intergenic (+150/+162) RCAP_rcc02161 → / ← mrcA hypothetical protein/penicillin‑binding protein 1A

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,330,1757.→C72.2% 49.9 0.0 0.054intergenic (+150/+162)RCAP_rcc02161/mrcAhypothetical protein/penicillin‑binding protein 1A
Reads supporting (aligned to +/- strand):  new base (4/35):  ref base (1/14):  total (5/49)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

GGCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......CGGCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCG  >  NC_014034/2330076‑2330245
                                                                                                     |                                                                            
ggCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGgc.gg                                                                              <  1:1082643/101‑2 (MQ=255)
 gCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGgc.ggg                                                                             >  1:4394266/1‑99 (MQ=255)
 gCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGgc.ggg                                                                             <  1:601698/101‑3 (MQ=255)
 gCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGgc.ggg                                                                             <  2:3271453/101‑3 (MQ=255)
  cTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggg                                                                     >  1:3903213/1‑101 (MQ=255)
  cTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggg                                                                     <  1:2030083/101‑1 (MQ=255)
  cTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggg                                                                     <  2:4400030/101‑1 (MQ=255)
   ttGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gggg                                                                    <  2:3436649/101‑2 (MQ=255)
    tGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGCC.G.......Ggggg                                                                   >  2:3824296/1‑99 (MQ=255)
     ggCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Gggggg                                                                  >  2:1786215/1‑98 (MQ=255)
       ccTCCGCCAAGGGCGGCACCGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctcc                                                                <  2:4346917/101‑6 (MQ=255)
       ccTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctcc                                                                <  2:3974262/101‑6 (MQ=255)
       ccTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctcc                                                                <  1:73698/101‑6 (MQ=255)
        cTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggggccc                                                               >  1:1961494/1‑95 (MQ=255)
         tCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctccgc                                                              <  2:3792962/101‑8 (MQ=255)
         tCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctccgc                                                              <  2:4403295/101‑8 (MQ=255)
           cGCCAAGGGGGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCAGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctccgccc                                                            <  2:1049312/101‑10 (MQ=37)
           cGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctccgccc                                                            <  2:4090765/101‑10 (MQ=38)
            gCCAAGGTCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctccgcccc                                                           <  2:3296802/101‑11 (MQ=37)
            gCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctccgcccc                                                           <  1:1887689/101‑11 (MQ=37)
            gCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......Ggggctccgcccc                                                           <  2:3294478/101‑11 (MQ=37)
             ccAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGCTG.......GGGCTCCGCCccc                                                           <  2:2661408/101‑1 (MQ=38)
                                 cAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTgcg                                             <  1:4183025/101‑1 (MQ=18)
                                  agagCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTgcgc                                            <  1:4269809/101‑1 (MQ=18)
                                  agagCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTgcgc                                            <  1:614274/101‑1 (MQ=18)
                                    agCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCa                                          <  2:3173422/101‑1 (MQ=18)
                                    agCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCa                                          <  1:2923123/101‑1 (MQ=18)
                                     gccccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCaa                                         <  1:3155748/98‑1 (MQ=18)
                                      cgccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGTCCCCGCACCCCCGGCGTATTTGCGCCAAg                                        <  2:3902959/101‑1 (MQ=21)
                                      cgccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAg                                        <  1:3367409/101‑1 (MQ=18)
                                      cgccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAg                                        <  2:3723166/101‑1 (MQ=18)
                                        ccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGaa                                      <  1:1377464/101‑1 (MQ=18)
                                         cgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGaaa                                     <  1:3363685/101‑1 (MQ=18)
                                           cACGGATCGGCCGATTGTGGAGAGGAATTTCGCCGCGCAGGCGCGGCGTCCCGGGC.GGGGGCTCCGCCCCCGCTCCCCCGGCGTATTTGCGCCAAGaaaaa                                   <  1:3690095/101‑1 (MQ=12)
                                           cACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCCCCCCCGGCGTATTTGCGCCAAGaaaaa                                   <  2:500791/101‑1 (MQ=17)
                                           cACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGaaaaa                                   <  2:3567426/101‑1 (MQ=17)
                                             cGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGc                                 <  1:3710638/101‑1 (MQ=18)
                                              ggttgggCCTATGGCGGAGAGGAATTTCGCCTCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGcc                                <  1:3142471/96‑1 (MQ=9)
                                              ggATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGcc                                <  2:2661258/101‑1 (MQ=18)
                                              ggATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGcc                                <  1:2692365/101‑1 (MQ=18)
                                                    gCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcg                          <  1:1572619/101‑1 (MQ=17)
                                                    gCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcg                          <  2:4341996/101‑1 (MQ=17)
                                                     ccGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcgc                         <  1:2169334/101‑1 (MQ=17)
                                                     ccGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCCCACCCCCGGCGTATTTGCGCCAAGAAAAAAACGGGgcgc                         >  1:191330/1‑101 (MQ=9)
                                                      cGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcgcg                        <  2:3020424/101‑1 (MQ=17)
                                                      cGATGGCGCAGAGGCATTTCGCCGCGCAGGCGCAGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcgcg                        <  1:3848669/101‑1 (MQ=9)
                                                         tGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCAt                     <  1:4005371/101‑1 (MQ=17)
                                                         tGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCAt                     <  1:2744976/101‑1 (MQ=17)
                                                          ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg                    <  1:684923/101‑1 (MQ=17)
                                                          ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg                    >  1:3254901/1‑101 (MQ=17)
                                                             ggAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCGCCCCCGGCGTATTTGCGCTAAGAAAAAGCCGGGGCGCGCATGCgg                 <  1:888535/101‑1 (MQ=12)
                                                             ggAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCgg                 <  2:340920/101‑1 (MQ=17)
                                                             ggAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCga                 <  2:3456097/101‑2 (MQ=17)
                                                              gagaGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCggg                <  2:2559754/101‑1 (MQ=17)
                                                              gagaGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCggg                >  2:4233911/1‑101 (MQ=17)
                                                                   gAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGcccc           <  2:1961365/101‑1 (MQ=17)
                                                                   gAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGcccc           >  2:1288734/1‑101 (MQ=17)
                                                                   gAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGCGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGcccc           <  2:2521389/101‑1 (MQ=18)
                                                                     aTTTCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCgg         <  1:1619891/101‑1 (MQ=17)
                                                                        tCGCCGCGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATc      <  2:2522010/101‑1 (MQ=18)
                                                                         cgccgcGCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCa     <  2:928724/101‑1 (MQ=18)
                                                                            cgcgCAGGCGCGGCGACCCGGGC.GGGGGCTCCGCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACcg  <  2:1168758/101‑1 (MQ=18)
                                                                                                     |                                                                            
GGCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC.G.......CGGCCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCG  >  NC_014034/2330076‑2330245

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG