Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,337,131 +G 2.9% intergenic (‑100/+476) rne ← / ← dctD ribonuclease E/C4‑dicarboxylate transport transcriptional regulatory protein DctD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,337,1311.→G 2.9% 2.5 ‑4.2 ‑0.2102intergenic (‑100/+476)rne/dctDribonuclease E/C4‑dicarboxylate transport transcriptional regulatory protein DctD
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (23/76):  total (26/76)
Fisher's exact test strand distribution p-value = 1.51e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.69e-01

Only 100 of 144 total aligned reads displayed.

TAAATCCTGTTTCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCCGCGCCGATT  >  NC_014034/2337031‑2337231
                                                                                                     |                                                                                                       
tAAATCCTGTTTCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATcggc                                                                                                          <  1:2012595/101‑1 (MQ=255)
 aaaTCCTGTTTCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCCGGCGCCCCTACGGATATcggc.g                                                                                                        >  1:1666354/1‑101 (MQ=255)
   aTCCTGTTTCCCCACGCCNCGTCGCACCTTNGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATcggc.ggc                                                                                                      <  1:1745976/101‑1 (MQ=255)
     ccTGTTTCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCCGGCGCCCATACGGATATcggc.ggggg                                                                                                    >  1:870314/1‑101 (MQ=255)
      cTGTTTCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGGGCCCATAAGGGTATcggc.gggggg                                                                                                   >  2:1712701/1‑100 (MQ=255)
        gTTTCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATcggc.ggcggcgg                                                                                                 <  2:2353851/101‑1 (MQ=255)
          ttCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGcc                                                                                               <  2:4081897/101‑1 (MQ=255)
          ttCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGcc                                                                                               <  1:2507460/101‑1 (MQ=255)
           tCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGGGCCCATACGGATATCGGC.GGGGGGGGGCg                                                                                              >  2:181830/1‑101 (MQ=255)
            tcccACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGGGCCCATACGGATATcggc.gggggggggggg                                                                                             >  2:2707855/2‑97 (MQ=255)
            ccccACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGa                                                                                             <  2:1558235/101‑1 (MQ=255)
             cccACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCCTACGGATATcggc.gggggggggggga                                                                                            >  2:3935372/1‑96 (MQ=255)
             cccACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCCTACGGATATCGGC.GGGGGCGGGCgga                                                                                            >  1:539064/1‑99 (MQ=255)
             cccACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGGGGGCgga                                                                                            >  1:4315090/1‑99 (MQ=255)
              ccACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGaaa                                                                                           <  1:3198365/101‑1 (MQ=255)
                cccccgcGTCGCACCTTGGCCTTGCGCGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGc                                                                                         <  2:3669942/98‑1 (MQ=255)
                aCGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGGGGGCGgaaag                                                                                         >  2:1094622/1‑99 (MQ=255)
                aCGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGc                                                                                         <  2:2418411/101‑1 (MQ=255)
                aCGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGc                                                                                         <  1:4274628/101‑1 (MQ=255)
                  gccgcGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.gg                                                                                      <  2:1926811/101‑1 (MQ=255)
                   ccgcGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGGGGGCggacagggg                                                                                      >  1:496667/1‑93 (MQ=255)
                     gcgTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.Ggccc                                                                                   <  1:2097399/101‑1 (MQ=255)
                        tCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.Ggcccgcc                                                                                <  2:1422147/101‑1 (MQ=255)
                        tCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.Ggcccgcc                                                                                <  1:1877630/101‑1 (MQ=255)
                         cGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.Ggcccgccc                                                                               <  2:2519413/101‑1 (MQ=255)
                         cGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.Ggcccgccc                                                                               <  2:2593269/101‑1 (MQ=255)
                         cGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.Ggcccgccc                                                                               <  2:854105/101‑1 (MQ=255)
                             ccTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGGTATCGGC.GGGGGCGGGCGAAAACGGGcccggccgcc                                                                            >  2:1840364/1‑99 (MQ=255)
                             ccTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGcccgcccgaag                                                                           <  2:3045391/101‑4 (MQ=255)
                               ttGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGtcatc                                                                         <  1:2963285/101‑1 (MQ=255)
                               ttGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGtcatc                                                                         <  2:4342812/101‑1 (MQ=255)
                                tGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGGGGGCGGAAGA.Ggggcgccccgccccc                                                                        >  2:1646322/1‑94 (MQ=255)
                                 ggCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGCGGGCGGAAAC.Gggcccccccccccccc                                                                       >  1:1166946/1‑93 (MQ=255)
                                  gCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCACCAcc                                                                      <  2:3769336/101‑1 (MQ=255)
                                   ccTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCAccc                                                                     <  1:917117/101‑1 (MQ=255)
                                    cTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCa                                                                    <  2:757459/101‑1 (MQ=255)
                                     ttGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAt                                                                   <  2:2306316/101‑1 (MQ=255)
                                       gCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.cc                                                                <  2:464356/101‑1 (MQ=255)
                                        cGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.ccc                                                               <  1:1840488/101‑1 (MQ=255)
                                        cGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.ccc                                                               <  1:1838318/101‑1 (MQ=255)
                                         gggCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCg                                                              <  2:1936198/101‑1 (MQ=255)
                                         gggCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCg                                                              <  1:3553762/101‑1 (MQ=255)
                                          ggCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGt                                                             <  1:968594/101‑1 (MQ=255)
                                            cAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTg                                                           <  2:3846283/101‑1 (MQ=255)
                                             aaGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGc                                                          <  2:1205441/101‑1 (MQ=255)
                                                gcgggcggGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGt                                                       <  1:1646428/101‑1 (MQ=255)
                                                gcgggcggGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGt                                                       <  2:456493/101‑1 (MQ=255)
                                                  gggcgggGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGGGGGCGGAAAG.GGCGCCCCCCCCCTCACCCCC.CCCCCTGCCGCGc                                                     >  2:982716/1‑101 (MQ=255)
                                                  gggcgggGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGc                                                     <  1:2957167/101‑1 (MQ=255)
                                                   ggcgggGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCa                                                    <  1:2186512/101‑1 (MQ=255)
                                                      ggggCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAAcc                                                 <  2:2875263/101‑1 (MQ=255)
                                                         gCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCAt                                              <  1:138552/101‑1 (MQ=255)
                                                          gaTGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATc                                             <  2:1133784/100‑1 (MQ=255)
                                                          cATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATc                                             <  1:1066433/101‑1 (MQ=255)
                                                           aTGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGGGGCCGGAAGCGGGCCCCCCCGCCCCAACCCAC.CCCGTTGCCTGCCAACCCacc                                             >  2:1532862/1‑99 (MQ=255)
                                                            tGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGGGGGCGGAAAC.GGGCCGCCCCCCCTCACCCCC.CCCCTTGCCCCGCCAcccccccc                                           >  1:2011948/1‑96 (MQ=255)
                                                               tttGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGCGGGGGGGGGCCGAAAGG.GGCCGCCCCGCCACCCCCCAC.CCCCTTCCCCGGCACCCCaccaggg                                         >  1:3503698/1‑95 (MQ=255)
                                                               tttGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGt                                        <  2:496615/101‑1 (MQ=255)
                                                                ttGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGtt                                       <  1:2828490/101‑1 (MQ=255)
                                                                    cGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGGGGCCGGAAGC.GGGCCCCCCCCCATCCCCCCC.CCCCCTTCCCTGCCACCCACcgccggtctgc                                   >  2:968144/1‑93 (MQ=255)
                                                                       tGAGGCTCGGGCGCCCATACGGATATCGGC.GGGTGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCa                               <  1:3801170/101‑1 (MQ=255)
                                                                       tGAGGCTCGGGCGCCCATACGGATATCGGC.GGGGGCGGGCGAAAGC.GGCCCGCCCCCCACCACCCCC.CCCCCCGCCGCGCCACCCCCCCCCGTTCG.Tcccc                               >  1:3163752/1‑100 (MQ=255)
                                                                       tGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCa                               <  1:416974/101‑1 (MQ=255)
                                                                       tGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCa                               <  1:4218895/101‑1 (MQ=255)
                                                                       tGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCa                               <  1:2508815/101‑1 (MQ=255)
                                                                        gAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCaa                              <  2:3221668/101‑1 (MQ=255)
                                                                        gAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCaa                              <  1:1406524/101‑1 (MQ=255)
                                                                        gAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCaa                              <  1:2900423/101‑1 (MQ=255)
                                                                         aGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAg                             <  1:1046405/101‑1 (MQ=255)
                                                                          ggCTCGGGCGCCCATCCGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAgc                            <  2:245582/101‑1 (MQ=255)
                                                                           gCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAAC.GGCCCGCCCCCCATCAACCAC.CCCCTTGCCGCGCAACCCACCCCGGGTCG.Tccccaacc                           >  2:1027535/1‑96 (MQ=255)
                                                                            cTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGGCCCCCCGTCATCACCCCT.CCCCCTGCCCTGCCACCCCACCCGGGTCG.TCCCCAgcgc                          >  2:2346688/1‑101 (MQ=255)
                                                                             tCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGcc                         <  2:2808200/101‑1 (MQ=255)
                                                                             tCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGcc                         <  1:3250223/101‑1 (MQ=255)
                                                                              cGGGCGCCCATACGGATATCGGT.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCg                        <  1:3087544/101‑1 (MQ=255)
                                                                               gggCGCCCATACGGATATCGGC.GGCGGGGGGCGGAAGA.GGCCCCCCCGCCCACACCCCTCCCCGTGGCCG.GCAACCCACCCCAGCTCGCCCCCAAgcgcgg                        >  1:2636229/1‑99 (MQ=255)
                                                                               gggCGCCCATACGGATATCGGC.GGCGGGGGGCGAAAGC.GGCCCCCCCCGCCTCACCCCT.CCCCCTGCCGTGCAACCCAACCCGGGTCG.GCCCCAACGcccg                       >  1:2679584/1‑99 (MQ=255)
                                                                               gggCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GTCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCgg                       <  2:2505236/101‑1 (MQ=255)
                                                                               gggCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCgg                       <  2:4264654/101‑1 (MQ=255)
                                                                               gggCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCCCCATCACCCCT.CCCGTTGCCGCGCCACCCACCCCGGGTCG.TCCCAACCGcccg                       >  1:2414228/1‑99 (MQ=255)
                                                                               gggCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAAC.GGCCCCCCCCCCATCCCCCAC.CCCCCTGCCGGGCAACCCCTCCCGGGTCG.TCCCaaccccccg                       >  1:1907088/1‑94 (MQ=255)
                                                                               gggCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAAA.GGCCCCCCCCCCATCACCCCT.CCCCGTGCCGGGCAACCCATCCCGGGTCG.TCCCCAACGcccg                       >  2:4074295/1‑99 (MQ=255)
                                                                                   gcCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCtt                   <  1:2385246/101‑1 (MQ=255)
                                                                                      cATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTc                <  1:2248287/101‑1 (MQ=255)
                                                                                      cATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCCCCCGGCATCAACCAT.CCCCTTGCCCTGCCACCCAACGCGGTTCG.TCCCCAACCCCCGGCTTGTc                >  2:725532/1‑101 (MQ=255)
                                                                                      cATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCCCCCCTCATCACCCCT.CCCCCTGCCCTGCAACCCATCGCGGGTCG.TCCCAAGCGCCCGGCCTGTc                >  1:2884237/1‑101 (MQ=255)
                                                                                       aTACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCg               <  2:4138922/101‑1 (MQ=255)
                                                                                        tACGGATATCGGC.GGCGGCGGCCGAAAGC.GGGCCGCCCCGCATCCCCCCT.CCCGCTGCCGGGCAAACCAACCCGGTTCG.CCCCAAACGCCCGGCTTGTCGc              >  2:1182574/1‑101 (MQ=255)
                                                                                         aCGGATATCGGC.GGCGGCGGCCGAAAAC.GGCCCCCCCCTCCTCACCCCT.CCCCCTGCCCCGCAAACCCACCCGGGTCG.TCCCCACCGCCCGGGCTGTCCcc             >  1:312464/1‑101 (MQ=255)
                                                                                          cGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGccc            <  1:1776469/101‑1 (MQ=255)
                                                                                            gATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCcg          <  2:4160708/101‑1 (MQ=255)
                                                                                            gATATCGGC.GGCGGCGGCCGAAAAA.GGGCCGCCCGCCATCACCCAT.CCCGCTGGCGTGCCACCCATCGCGGGTCG.TCCCAACCGCCCGGCGTGTTGCCCcg          >  2:1581573/1‑101 (MQ=255)
                                                                                             atatCGGC.GGCGGCGGCCGAAAGC.GGGCCCCCCGCCATCACCCCT.CCCCTTGCCGTGCAACCCATCGCGGGTCG.TCCCAAGAGCCGGGGCTGTCGcccccc         >  2:401275/1‑99 (MQ=255)
                                                                                               atCGGC.GGCGGCGGCCGNAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCgatgccacc       <  2:169426/101‑9 (MQ=255)
                                                                                                 cggc.ggcggcggCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCCGCGCCg     >  1:3420157/1‑101 (MQ=255)
                                                                                                 cggc.ggcggcggCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCCGCGCCg     >  2:2917435/1‑101 (MQ=255)
                                                                                                  ggc.ggcggcggcCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCCGCGCCgc    >  2:805773/1‑100 (MQ=255)
                                                                                                  ggc.ggcggcggcCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCCGCGCCGa    >  1:777428/1‑101 (MQ=255)
                                                                                                  ggc.ggcggcggcCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCCGCGCCGa    >  1:1779102/1‑101 (MQ=255)
                                                                                                   gc.ggcggcggcCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCCGCGCCGAt   <  1:4319103/101‑1 (MQ=255)
                                                                                                     |                                                                                                       
TAAATCCTGTTTCCCCACGCCGCGTCGCACCTTGGCCTTGCGGGCAAGGCGGGCGGGGCATGGTTTGTCGTTGAGGCTCGGGCGCCCATACGGATATCGGC.GGCGGCGGCCGAAAGC.GGCCCGCCCGTCATCACCCAT.CCCGTTGCCGTGCAACCCATCGCGGTTCG.TCCCAAGCGCCGGGCTTGTCGCCCCGCGCCGATT  >  NC_014034/2337031‑2337231

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG