BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,472,573	+G	8.7%	intergenic (‑158/+25)	RCAP_rcc02296 ← / ← RCAP_rcc02297	GTP‑binding protein Obg/CgtA/GNAT family acetyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,472,573	0	.→G	8.7%	28.5 ‑0.5 ‑1.9	125	intergenic (‑158/+25)	RCAP_rcc02296/RCAP_rcc02297	GTP‑binding protein Obg/CgtA/GNAT family acetyltransferase
Reads supporting (aligned to +/- strand): new base (10/0): ref base (101/13): total (112/13)
Fisher's exact test strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.38e-02

Only 100 of 147 total aligned reads displayed.


TGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGGGAAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATCGAGC  >  NC_014034/2472479‑2472670


tGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgc                                                                                           >  1:671977/1‑101 (MQ=255)


tGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgc                                                                                           >  2:43461/1‑101 (MQ=255)


 gggggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgcg                                                                                          >  1:2205294/1‑101 (MQ=255)


 gggggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgcg                                                                                          <  1:1620589/101‑1 (MQ=255)


   gggCTCTGCCCCCAGACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGcc                                                                                        <  1:4424603/101‑1 (MQ=255)


    ggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGccc                                                                                       >  1:3928984/1‑101 (MQ=255)


     gCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCt                                                                                      >  1:3716982/1‑101 (MQ=255)


     gCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCt                                                                                      >  1:3435783/1‑101 (MQ=255)


     gCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCt                                                                                      >  2:1666640/1‑101 (MQ=255)


      ctctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTc                                                                                     >  2:3667054/1‑101 (MQ=255)


      ctctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTc                                                                                     >  1:2139891/1‑101 (MQ=255)


      ctctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTc                                                                                     >  1:3349211/1‑101 (MQ=255)


       tctGGCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTcc                                                                                    >  2:3768166/1‑101 (MQ=255)


        ttGCCCCCAAACCCCCGGGATATCTGAAGCAATATGAAGGTCCTTGCTTTCATCTTTCCTCAAATATTCCTGGTGGGGTTCGGGG..AGGGCAGCGCCCTccc                                                                                   <  1:2324410/100‑1 (MQ=255)


        ctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTccc                                                                                   >  2:4159365/1‑101 (MQ=255)


        ctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTccc                                                                                   <  2:1358053/101‑1 (MQ=255)


         tGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTcccc                                                                                  <  2:549999/101‑1 (MQ=255)


         tGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTcccc                                                                                  <  2:3483994/101‑1 (MQ=255)


          gCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATTTCCCGGGTGGGGTTC..GGGAAGGACAGCGCCCTCCCCg                                                                                 >  2:2956301/1‑101 (MQ=37)


          gCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCg                                                                                 >  2:2641585/1‑101 (MQ=255)


          gCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCg                                                                                 >  2:1789533/1‑101 (MQ=255)


              ccAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCCTCTTGCCTCAACTACCCCGGGGGGGGTTCGGCG..AGGGCAGCGCCCTCCCCGcccg                                                                             >  2:1054259/1‑100 (MQ=25)


              ccAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCt                                                                             >  1:2786225/1‑101 (MQ=255)


               cAACCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCtt                                                                            <  2:466537/101‑1 (MQ=255)


               cAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCtt                                                                            <  1:2825988/101‑1 (MQ=255)


               cAAACCCCCGGGATATTGGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCtt                                                                            <  2:2396425/101‑1 (MQ=255)


               aaaccccccGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATACCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCtt                                                                            <  2:4018723/97‑1 (MQ=37)


                aaaCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTg                                                                           >  2:3413171/1‑101 (MQ=255)


                aaaCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATATTGCCTCAAATATCCCGGGCAGGGATCGGGG..CGGGCAGCGCCCTCCCCGCCCTTg                                                                           >  2:1785274/1‑101 (MQ=25)


                   cccccGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCa                                                                        >  1:759269/1‑101 (MQ=255)


                    ccccGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAg                                                                       >  2:3013081/1‑101 (MQ=255)


                    ccccGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAg                                                                       >  1:2070316/1‑101 (MQ=255)


                       cGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCt                                                                    >  2:4344729/1‑101 (MQ=255)


                       cGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCt                                                                    >  2:1008996/1‑101 (MQ=255)


                       cGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCt                                                                    >  1:2317807/1‑101 (MQ=255)


                         gtatatTTGCAGAAAGAGGAGGGGCGTGGTTTTCTTCTT.CC.CAAAAATCCCGGGGGGGGGTTTGGGGGGGGGCCGCCCCCCCCCCCCCCTTGTCAGCCTgc                                                                  <  2:722265/99‑1 (MQ=255)


                         ggATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTgc                                                                  >  1:2438395/1‑101 (MQ=255)


                         ggATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTgc                                                                  >  2:1887534/1‑101 (MQ=255)


                          gATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTgcg                                                                 >  1:3555037/1‑101 (MQ=255)


                          gATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTgcg                                                                 >  1:2897910/1‑101 (MQ=255)


                           atatTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCCGCCTgcgc                                                                >  1:1321304/1‑101 (MQ=255)


                            tatTGGCAGAAAGAGGAGGGGCCTTTCTTTAATCTT.CCCCAAAT.TCCCGGGGGGGGGGTGGGGGGGGGCCGCCCCCCCCCCCGCCCTTGTCAGCCTGCGCa                                                               <  1:4011394/101‑1 (MQ=255)


                              tttGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAgg                                                             >  1:3515987/1‑101 (MQ=255)


                              tttGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAgg                                                             >  2:841202/1‑101 (MQ=255)


                              tttGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCCCCCTTGTCAGCCTTCGCAgc                                                             >  1:1593492/1‑100 (MQ=38)


                                tgcggaaaGAGGAGGGCCCGTTTTTTCATCTCGCCTAAAATA.CCCGGGGGGGGGT.GGGGGGGGGCCGGCCCCCCCCCCCCCCTTGTCAGCCTGCGCGggcg                                                           <  2:2786035/95‑1 (MQ=255)


                                 gaagaaaGAGGAGGGGCGGTGCTTTCATC.TGCC.CAAAAATCCCGGGGGGGGGGTGGGGGGGGGCCCCCCCCCCCCCCCCCCTTGTCAGCCTGCGCAggcgg                                                          <  2:2003673/96‑1 (MQ=255)


                                  cagcaAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAggcggc                                                         >  2:4368609/1‑101 (MQ=255)


                                     caAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTc                                                      >  1:1724629/1‑101 (MQ=255)


                                      aaGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCa                                                     >  1:3522756/1‑101 (MQ=255)


                                       aGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCaa                                                    >  2:730317/1‑101 (MQ=255)


                                        gaggagggcccGTTCTTTC.TTTTTCCTAAAATTCCCCGGGGGGGGTT.GGGGGGGGGCCCCCCCCCCCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGCCaaa                                                   <  2:640171/92‑1 (MQ=255)


                                        gATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCaaa                                                   >  1:2525259/1‑101 (MQ=255)


                                         aTGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAg                                                  >  1:413305/1‑101 (MQ=255)


                                           gagggcccttGCTTTC.TCCTGCCCCCAACCTCCCGGGGGGGGGT.GGGGGGCGGGCACCGCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggc                                                <  2:2523905/92‑1 (MQ=255)


                                            aaGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcg                                               >  1:659611/1‑101 (MQ=255)


                                            aaGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcg                                               >  2:3323367/1‑101 (MQ=255)


                                            aaGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcg                                               >  2:1568735/1‑101 (MQ=255)


                                             aGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcgg                                              >  2:2209244/1‑101 (MQ=255)


                                             aGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcgg                                              >  2:1372171/1‑101 (MQ=255)


                                             aGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcgg                                              >  2:931958/1‑101 (MQ=255)


                                                gCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgc                                           >  1:697565/1‑101 (MQ=255)


                                                 ccGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcg                                          >  1:2853341/1‑101 (MQ=255)


                                                 ccGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCACCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcg                                          >  2:2113635/1‑101 (MQ=255)


                                                  gtttttttCTTCTTCCCT.AAAT.TCGCGGGGGGGGGTGGGGGGGGGGGCCGCCCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcgg                                         <  1:1694232/96‑1 (MQ=255)


                                                     gCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtc                                      >  1:2205406/1‑101 (MQ=255)


                                                      cTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtca                                     >  1:960434/1‑101 (MQ=255)


                                                        ttCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagt                                   >  1:4213929/1‑101 (MQ=255)


                                                        ttCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagt                                   >  1:1474000/1‑101 (MQ=255)


                                                         tCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtt                                  >  1:1154294/1‑101 (MQ=255)


                                                         tCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtt                                  >  2:2196771/1‑101 (MQ=255)


                                                          cATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGGCTGCGCCGGCGGCGTCAAAGGCGGCGCGGCtcagttt                                 >  2:1034042/1‑101 (MQ=255)


                                                          cATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagttt                                 >  2:2438636/1‑101 (MQ=255)


                                                          cATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagttt                                 >  2:3862655/1‑101 (MQ=255)


                                                           aTCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttc                                >  1:840551/1‑101 (MQ=255)


                                                           aTCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttc                                >  1:2750823/1‑101 (MQ=255)


                                                             cTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCGCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcag                              >  2:2271382/1‑101 (MQ=255)


                                                             cTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcag                              >  1:2823524/1‑101 (MQ=255)


                                                             cTTGCCTCAAATATCCCGGGGGGGGTCCGGGGG..GGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGGGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcag                              >  1:1034838/1‑101 (MQ=38)


                                                               tGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagtt                            >  1:2529709/1‑101 (MQ=255)


                                                               tGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagtt                            >  1:4340522/1‑101 (MQ=255)


                                                                gCCTCAAATATCGCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTc                           >  1:2430355/1‑101 (MQ=255)


                                                                gCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTc                           >  2:3709745/1‑101 (MQ=255)


                                                                    cAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCcaca                       >  2:4420462/1‑101 (MQ=255)


                                                                     aaaTATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACAt                      >  1:4159376/1‑101 (MQ=255)


                                                                      aaTATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATg                     >  1:1382107/1‑101 (MQ=255)


                                                                        tatCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCg                   >  2:3606506/1‑101 (MQ=255)


                                                                          tCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGc                 >  2:2503125/1‑101 (MQ=255)


                                                                                  ggggTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGc         >  1:1001357/1‑101 (MQ=255)


                                                                                  ggggTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGc         >  1:2294488/1‑101 (MQ=255)


                                                                                  ggggTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGc         >  2:2873534/1‑101 (MQ=255)


                                                                                       gttcgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATc      >  1:3758235/5‑101 (MQ=255)


                                                                                       gcGGGGGGGGGGCACCCCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATc      <  1:3441408/100‑1 (MQ=255)


                                                                                        ttcgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcg     >  1:1444587/4‑101 (MQ=255)


                                                                                          cgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgag   >  2:1699919/2‑101 (MQ=255)


                                                                                          cgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgag   >  2:610242/2‑101 (MQ=255)


                                                                                          cgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgag   >  2:1704647/2‑101 (MQ=255)


                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgagc  >  1:4193463/1‑101 (MQ=255)


                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgagc  >  2:1967971/1‑101 (MQ=255)


                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTAAGTTCCACATGCGGCAGGATCGCATcgagc  >  1:298106/1‑101 (MQ=255)


TGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGGGAAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATCGAGC  >  NC_014034/2472479‑2472670

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG