Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,486,442 +C 3.6% intergenic (‑70/+124) RCAP_rcc02312 ← / ← soxG hypothetical protein/sarcosine oxidase subunit gamma

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,486,4420.→C 3.6% 18.5 ‑1.2 ‑1.4222intergenic (‑70/+124)RCAP_rcc02312/soxGhypothetical protein/sarcosine oxidase subunit gamma
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (93/121):  total (98/124)
Fisher's exact test strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.00e-02

Only 100 of 230 total aligned reads displayed.

GCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.A.GGGGCGCTGCCCCTCTCTTG.CCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAGTCCGCAG  >  NC_014034/2486347‑2486538
                                                                                                                                                                                                   
gcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCtt                                                                                              <  1:5461/101‑1 (MQ=255)
gcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCtt                                                                                              <  2:3350441/101‑1 (MQ=255)
 cgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTtg                                                                                             >  2:2976899/1‑101 (MQ=255)
  gcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTtg.c                                                                                           >  2:461445/1‑101 (MQ=255)
    ccccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTtg.ccc                                                                                         <  1:985267/101‑2 (MQ=255)
    ccccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTtg.ccc                                                                                         <  1:1320992/101‑2 (MQ=255)
     cccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTtg.ccct                                                                                        <  2:2295143/101‑3 (MQ=255)
        ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTtg.ccctgcc                                                                                     >  1:3837583/1‑96 (MQ=255)
        ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTtg.ccctgcc                                                                                     >  1:1203476/1‑96 (MQ=255)
          aTTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTtg.ccccgccgg                                                                                   >  1:3986618/1‑94 (MQ=255)
          aTTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCgg                                                                                    >  1:1525392/1‑101 (MQ=255)
            ttCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGc                                                                                  >  2:4236492/1‑101 (MQ=38)
             tCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCa                                                                                 >  1:92196/1‑101 (MQ=38)
              cGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCaa                                                                                >  1:4111990/1‑101 (MQ=38)
               gAATAGTCCATGCTGGCTCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                               <  1:1991859/101‑1 (MQ=38)
               gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                               >  1:2608988/1‑101 (MQ=38)
               gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                               >  1:4275376/1‑101 (MQ=38)
                  tAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCa                                                                            <  1:221315/101‑1 (MQ=38)
                  tAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCa                                                                            >  1:1358784/1‑101 (MQ=38)
                     tCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAccc                                                                         >  2:2792661/1‑101 (MQ=37)
                     tCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAccc                                                                         <  1:1901820/101‑1 (MQ=37)
                       cATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAccccc                                                                       <  2:2470276/101‑1 (MQ=37)
                          gCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCggg                                                                    <  2:1768851/101‑1 (MQ=37)
                          gCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCgga                                                                    >  1:2009802/1‑100 (MQ=37)
                           cTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGa                                                                   <  1:2113209/101‑1 (MQ=37)
                            tGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGat                                                                  <  2:2708037/101‑1 (MQ=37)
                            tGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGat                                                                  >  2:4372033/1‑101 (MQ=37)
                             ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGgta                                                                 >  1:782767/1‑101 (MQ=37)
                             ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                                 <  1:638353/101‑1 (MQ=37)
                             ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                                 <  1:4434019/101‑1 (MQ=37)
                             ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                                 >  2:4159787/1‑101 (MQ=37)
                             ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                                 <  2:2598767/101‑1 (MQ=37)
                              gATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGatat                                                                >  2:2108079/1‑101 (MQ=37)
                              gATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGatat                                                                <  1:4388551/101‑1 (MQ=37)
                                tctcCTTTGCCTTCGATCTGCGGCAACCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTc                                                              <  1:1761684/101‑1 (MQ=25)
                                 ctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCa                                                             >  2:571405/1‑101 (MQ=37)
                                  tcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCTGGCAAGTCACCCCCGGGATATTCAt                                                            <  2:3806148/101‑1 (MQ=37)
                                   ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATg                                                           >  2:1127763/1‑101 (MQ=37)
                                   ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATg                                                           >  2:2181708/1‑101 (MQ=37)
                                   ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATg                                                           <  2:734126/101‑1 (MQ=37)
                                    cTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATgg                                                          >  2:3044381/1‑101 (MQ=37)
                                      ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                                        <  1:2459897/101‑1 (MQ=37)
                                      ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                                        >  2:1672803/1‑101 (MQ=37)
                                        gCCTTCGATCTGCGGCTAGCCCGGCCGCAAGGCAAGCGAAACG.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAg                                                      <  1:2977344/101‑1 (MQ=25)
                                        gCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAg                                                      <  1:2340645/101‑1 (MQ=37)
                                        gCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAg                                                      <  2:222763/101‑1 (MQ=37)
                                        gCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAg                                                      <  1:2700726/101‑1 (MQ=37)
                                         ccTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGa                                                     <  2:3986362/101‑1 (MQ=37)
                                          cTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGAt                                                    <  2:3476287/101‑1 (MQ=37)
                                          cTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGAt                                                    <  1:302674/101‑1 (MQ=37)
                                           ttCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATg                                                   <  1:1242401/101‑1 (MQ=37)
                                           ttCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATg                                                   <  2:603900/101‑1 (MQ=37)
                                              gATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAag                                                >  2:1402817/1‑101 (MQ=37)
                                               aTCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagg                                               >  1:89161/1‑101 (MQ=37)
                                                tCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagga                                              <  2:328344/101‑1 (MQ=37)
                                                 cTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCCCCCCCGGGATATTCATGGCAAGATGAaggag                                             >  1:961538/1‑101 (MQ=25)
                                                 cTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAaggag                                             >  1:4060134/1‑101 (MQ=25)
                                                 cTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAaggag                                             <  2:1235260/101‑1 (MQ=37)
                                                  tGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAaggagg                                            <  2:4386653/101‑1 (MQ=37)
                                                   gCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGc                                           <  1:1131592/101‑1 (MQ=37)
                                                      gCAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgtg                                        >  1:3320423/1‑101 (MQ=25)
                                                       cAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgtgt                                       <  1:571473/101‑1 (MQ=37)
                                                       cAAGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgtgt                                       <  2:3174382/101‑1 (MQ=37)
                                                         aGCCCGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGttt                                     <  2:4111730/101‑1 (MQ=37)
                                                            ccGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCAt                                  <  2:2251770/101‑1 (MQ=25)
                                                            ccGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAAGCgaagcgacg                                  <  2:1920198/101‑9 (MQ=21)
                                                             cGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATc                                 <  1:3764444/101‑1 (MQ=25)
                                                             cGGCCGCAAGGCAAGCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGAAACCCCCGGGAACTTCATGGCAAGAAAAAAGAGGCGTGTTTCGTc                                 >  1:3946201/1‑101 (MQ=19)
                                                                  gcaaggcaagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGcc                            >  2:2501563/1‑101 (MQ=21)
                                                                     aggcaagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCCCCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATa                         >  1:2529542/1‑101 (MQ=21)
                                                                     aggcaagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATa                         <  1:1015632/101‑1 (MQ=21)
                                                                     aggcaagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATAGCAAGATGAAGGAGGCGTGTATCATCTTGCCATa                         >  1:2197012/1‑101 (MQ=21)
                                                                       gcaagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaaa                       <  1:2418778/101‑1 (MQ=25)
                                                                       gcaagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaaa                       <  2:2634246/101‑1 (MQ=25)
                                                                       gcaagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaaa                       <  1:3400028/101‑1 (MQ=25)
                                                                        caagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAat                      >  1:2473337/1‑101 (MQ=21)
                                                                        caagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAat                      <  2:1810673/101‑1 (MQ=21)
                                                                        caagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAat                      >  2:2740744/1‑101 (MQ=21)
                                                                        caagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAat                      <  1:4160048/101‑1 (MQ=21)
                                                                        caagCGAAA.AGGGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAat                      <  1:1063324/101‑1 (MQ=25)
                                                                           cgaaaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGCTTCATCTTGCCATAAATATcc                  >  1:2892222/4‑101 (MQ=25)
                                                                            gaaaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATTTGAAGATGAAGGAGGCGTGTTTCATTTTTCCATAAatatttc                 >  2:3890535/3‑98 (MQ=18)
                                                                            gaaaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATccc                 <  1:1704079/99‑1 (MQ=21)
                                                                            gaaaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCAAAAATATccc                 >  2:885260/3‑101 (MQ=18)
                                                                             aaaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCg                <  1:3630482/100‑1 (MQ=21)
                                                                             aaaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCg                >  2:1996943/2‑101 (MQ=21)
                                                                              aaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgg               <  1:39121/101‑1 (MQ=21)
                                                                              aaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgg               <  1:400426/101‑1 (MQ=21)
                                                                              aaa.g.GGGGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgg               >  1:2716616/1‑101 (MQ=21)
                                                                              aaa.a.GGGGGGCCGCCGCCCTCTTGCCCTGCCGGGCAAGTCGCCCCCGGGATAGTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCg                <  2:503746/101‑1 (MQ=21)
                                                                                 aagggggCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCggg              <  2:782685/98‑1 (MQ=21)
                                                                                  agggggCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgggg             >  2:3224213/3‑101 (MQ=21)
                                                                                    ggggCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgggggg           <  1:2801909/101‑1 (MQ=255)
                                                                                     gggCGCTGCCCC.CTCTTGCCCGGCCGGGCAAGTCGCCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGa          <  2:1159822/101‑1 (MQ=21)
                                                                                      ggCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAg         >  1:3142830/1‑101 (MQ=255)
                                                                                       gcccTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAGt        <  1:3471425/98‑1 (MQ=21)
                                                                                        cgcTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAGTc       <  1:461485/101‑1 (MQ=255)
                                                                                          cTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAGTCCg     >  2:1842670/1‑101 (MQ=255)
                                                                                          cTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAGTCCg     >  2:2373963/1‑101 (MQ=255)
                                                                                            tgcccCCTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAGTCCGc    <  1:2847772/99‑1 (MQ=255)
                                                                                                                                                                                                   
GCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAA.A.GGGGCGCTGCCCCTCTCTTG.CCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAGTCCGCAG  >  NC_014034/2486347‑2486538

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG