Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,060 +A 9.4% intergenic (+108/‑134) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0602.→A 9.4% 44.0 ‑2.0 ‑0.7128intergenic (+108/‑134)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/8):  ref base (66/50):  total (70/58)
Fisher's exact test strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.12e-01

Only 100 of 138 total aligned reads displayed.

CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493960‑2494152
                                                                                                      |                                                                                                   
cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                      <  1:2374334/101‑1 (MQ=255)
cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                      <  2:1030138/101‑1 (MQ=255)
 gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                                   <  1:3097965/101‑1 (MQ=255)
   tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                                <  1:3568460/101‑2 (MQ=255)
   tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                                <  1:2144125/101‑2 (MQ=255)
   tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                                <  2:2495794/101‑2 (MQ=255)
    gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                               <  1:1081080/101‑3 (MQ=255)
     cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCCGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..G.aagcc                                                                                             >  1:2946572/1‑97 (MQ=18)
     cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                              <  1:2808866/101‑4 (MQ=255)
     cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagc                                                                                              >  2:3866505/1‑98 (MQ=18)
      ccccG.TCCTCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                            <  1:1331605/101‑6 (MQ=18)
      ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                            <  1:1897539/101‑6 (MQ=255)
        cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                           <  1:3438156/97‑7 (MQ=255)
        cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                           >  1:3570867/5‑95 (MQ=255)
        cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                           <  1:769318/97‑7 (MQ=255)
        cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                           <  2:1226321/97‑7 (MQ=255)
          cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                         <  1:573662/99‑9 (MQ=255)
          cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                         >  1:3401873/3‑93 (MQ=255)
           gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagagg                                                                                        >  1:1827773/2‑92 (MQ=21)
            tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                                                       <  1:1724067/101‑11 (MQ=255)
            tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagaggg                                                                                       >  2:3007661/1‑91 (MQ=18)
                 aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                                                                >  2:2302328/1‑101 (MQ=18)
                 aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                                                                >  1:4364755/1‑101 (MQ=18)
                  aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCag                                                                               <  1:2028967/101‑2 (MQ=18)
                  aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCag                                                                               <  2:4028881/101‑2 (MQ=18)
                   gCGGACTCCCCCCGGGATTTTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagc                                                                              <  2:4145191/101‑3 (MQ=18)
                     ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                                                            >  1:815323/1‑97 (MQ=17)
                        cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cc                                                                      >  2:688702/1‑101 (MQ=17)
                         tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCG.cc.ccc                                                                     <  2:4142574/101‑1 (MQ=17)
                          ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccc                                                                    >  1:2365263/1‑100 (MQ=17)
                          ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccc                                                                    <  2:1952332/101‑2 (MQ=17)
                           cccccGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccct                                                                   <  1:19700/101‑3 (MQ=17)
                            ccccGGGATATGTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccctg                                                                  <  2:1322038/101‑4 (MQ=17)
                               cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGACC.CCCTGccc                                                                >  2:1214419/1‑101 (MQ=17)
                               cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATACCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGccc                                                                >  1:2563268/1‑101 (MQ=17)
                                gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGcccc                                                               >  1:2784255/1‑101 (MQ=0)
                                gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGcccc                                                               >  2:1325244/1‑101 (MQ=18)
                                gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGcccc                                                               >  1:576951/1‑101 (MQ=17)
                                gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGcccc                                                               <  1:3208022/101‑1 (MQ=17)
                                 ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcg                                                              >  2:1447132/1‑101 (MQ=0)
                                 ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.GGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcg                                                              >  1:4009471/1‑101 (MQ=17)
                                 ggATANNTGCAGAAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcg                                                              <  1:1594892/101‑1 (MQ=17)
                                  gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                             >  2:1683206/1‑100 (MQ=2)
                                   gtatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                            <  2:1429237/100‑3 (MQ=2)
                                   atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                            <  2:2819530/101‑3 (MQ=2)
                                   atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                            <  1:1155047/101‑3 (MQ=2)
                                    tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcggcg                                                           >  2:3118786/1‑101 (MQ=18)
                                    tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCACcggcg                                                           >  2:341801/1‑101 (MQ=17)
                                     atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                                                          <  2:337533/101‑1 (MQ=2)
                                     atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                                                          >  1:947985/1‑101 (MQ=17)
                                     atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GACAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                                                          >  1:477110/1‑101 (MQ=18)
                                       ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                                                        >  2:91130/1‑99 (MQ=2)
                                       ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                                                        <  2:3706895/101‑1 (MQ=17)
                                        tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.CCGCCCTGCCCCGGCGGTcc                                                       >  2:1376656/1‑101 (MQ=18)
                                         gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCg                                                      >  2:3410585/1‑101 (MQ=18)
                                          cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGTGC.AGCGCCC.CCCTGCCCCGGCGGTCCGt                                                     >  1:1490453/1‑101 (MQ=18)
                                          cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCCGCGGGCCgg                                                     >  2:3847046/1‑100 (MQ=14)
                                           agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTg                                                    >  1:3046297/1‑101 (MQ=17)
                                            gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                                                   >  1:1966347/1‑101 (MQ=17)
                                             caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                  >  2:503747/1‑101 (MQ=17)
                                              aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                                                 <  2:1110206/101‑1 (MQ=17)
                                              aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                                                 >  2:2922315/1‑101 (MQ=18)
                                                gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAA.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcggc                                               <  1:2171140/101‑1 (MQ=18)
                                                  tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc                                             <  2:225445/101‑1 (MQ=17)
                                                  tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc                                             >  2:4269411/1‑101 (MQ=17)
                                                  tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.CCTCCCTGCCCCGGCGGTCCGTGGCGgggc                                             >  1:414463/1‑101 (MQ=17)
                                                     aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  1:1249351/101‑1 (MQ=17)
                                                      aCCGGATGCCTTTCATCTTGCCCGAAATATCCTGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         >  2:3138641/1‑101 (MQ=18)
                                                      aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  1:2335769/101‑1 (MQ=17)
                                                       ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTgc                                        >  1:3157711/1‑100 (MQ=17)
                                                        cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGAGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       >  2:3217971/1‑101 (MQ=18)
                                                        cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.ACG.CCAG.AGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       >  2:2470170/1‑101 (MQ=18)
                                                        cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGAc                                       >  1:2467268/1‑101 (MQ=17)
                                                        cGGATGCCTTTCATCTTGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  1:1762593/101‑1 (MQ=17)
                                                         ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGaag                                      >  2:590309/1‑99 (MQ=17)
                                                            tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCACCGC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                   <  1:2541303/101‑1 (MQ=18)
                                                              ccTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                 <  2:2365113/101‑1 (MQ=17)
                                                              ccTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.GGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                 <  1:4447110/101‑1 (MQ=17)
                                                                 ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              >  2:109218/1‑101 (MQ=18)
                                                                  tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             >  2:940965/1‑101 (MQ=18)
                                                                   cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGGGCCGCCTTGAGGGGCGGGGgcg                            >  2:1158134/1‑101 (MQ=17)
                                                                   cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  2:1887942/1‑101 (MQ=18)
                                                                    aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggggg                           >  1:3265848/1‑98 (MQ=18)
                                                                    aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                           >  1:4316494/1‑101 (MQ=18)
                                                                    aTCTTGCCCGAAATATCCCGGGGGGTGAA.TAGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                           <  1:621085/101‑1 (MQ=18)
                                                                      cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAGGGGCGGGGGGGCgg                         >  1:2783674/1‑101 (MQ=17)
                                                                         gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggggatcggaa                      >  2:3740502/1‑93 (MQ=17)
                                                                           ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:3502492/101‑1 (MQ=18)
                                                                           ccGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    >  2:2103695/1‑101 (MQ=18)
                                                                            ggAAGTATCCGGGGGGGTGAA.TGGC..GAA.G.CCGG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                   <  1:1523195/100‑1 (MQ=21)
                                                                                   tcggggggggTGAA.TGGC..G.AGG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:3249841/97‑1 (MQ=25)
                                                                                     ccGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          >  2:1775408/1‑101 (MQ=21)
                                                                                     ccGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  2:3162691/101‑1 (MQ=21)
                                                                                      cGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAACGAAAttc         >  2:683660/1‑100 (MQ=21)
                                                                                        gggggTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  2:1844584/1‑101 (MQ=25)
                                                                                        gggggTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  2:762742/1‑101 (MQ=25)
                                                                                           ggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  1:3944784/101‑1 (MQ=25)
                                                                                            gTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg   <  1:744363/101‑1 (MQ=25)
                                                                                             tGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa  <  2:2936647/101‑1 (MQ=25)
                                                                                             tGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa  >  1:4331794/1‑101 (MQ=25)
                                                                                                      |                                                                                                   
CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493960‑2494152

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG